MythA.00999.a

Shikimate 5-dehydrogenase (EC 1.1.1.25) (Rv2552c ortholog)

CENTER ID: MythA.00999.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.00999.a.B1.GE37644 full length 1 255
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.1942
UniProt: G7CG49

Orthologues for Rv2552c

Species % Identity % Coverage Clones Proteins Structures
avium 84 97 0 0 0
marinum 83 98 0 0 0
paratuberculosis 82 100 1 0 0
ulcerans 82 98 0 0 0
leprae 81 99 0 0 0
smegmatis 74 97 0 0 0
fortuitum 72 97 1 0 1
thermoresistibile 71 95 1 0 0
abscessus 59 97 1 1 0
fortuitum 31 88 0 0 0
smegmatis 30 87 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLGSPIAHSR SPQLHLAAYR ALGLTGWTYE RIECDAEQLP VLVGGFGPEW VGVSVTMPGK FAALTFADQR TARAELVGSA NTLVRRDGGW LADNTDIDGV KGALGAVGEH VMVVGSGGTA PAAVVAVVES GARQVTVAAR NREKAAPLVE LAERLGASAR WQDLAGVTAA GVDAVVSTIP ADAAARYAAA LSATPVLVDA IYDPWPTPLA AAVAEAGGRV ISGLEMLLNQ AFTQVELFTG RPAPKEAMRA ALNQP
NT Sequence
gtgctgggat cgccgatcgc gcactcccgg tctccgcaac tgcatctggc cgcctaccgc gcgctcggcc tgaccggctg gacctacgaa cgcatcgaat gcgacgccga gcagctgccc gtgctcgtgg gcggtttcgg cccggaatgg gtcggggtgt cggtgacgat gcccggcaag ttcgccgccc tgacgttcgc cgatcagcgc accgcgcgcg ccgagctggt cggatcggcc aacaccctgg tgcgccggga cggcggctgg ctggccgaca acaccgacat cgacggtgtc aagggcgcgc tgggcgccgt cggtgagcat gtgatggtgg tcggttcggg cggcaccgct cccgcggcgg tggtcgcggt ggtcgagtcg ggcgcccggc aggtgaccgt cgccgcccgc aatcgtgaga aggccgcccc gttggtggaa ctggccgagc ggctgggcgc ctcggcccgt tggcaggacc tcgccggggt cacggccgcc ggggtcgacg cggtggtgag cacgattccc gccgatgcgg ccgcccggta cgcggcggcg ctgtcggcca cgccggtgct ggtggacgcg atctacgatc cgtggccgac gccgctggcc gcggccgtgg ccgaagccgg cggtcgggtg atcagcgggc tggagatgct gctgaatcag gcgttcaccc aggtcgagtt gttcaccggc cggcccgcgc ccaaagaagc gatgagggcg gcgctgaacc agccatag
Details for MythA.00999.a.B1.GE37644
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 27kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 93
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMV LGSPIAHSRS PQLHLAAYRA LGLTGWTYER IECDAEQLPV LVGGFGPEWV GVSVTMPGKF AALTFADQRT ARAELVGSAN TLVRRDGGWL ADNTDIDGVK GALGAVGEHV MVVGSGGTAP AAVVAVVESG ARQVTVAARN REKAAPLVEL AERLGASARW QDLAGVTAAG VDAVVSTIPA DAAARYAAAL SATPVLVDAI YDPWPTPLAA AVAEAGGRVI SGLEMLLNQA FTQGRVVHRP ARAQRSDEGG AEPAISE
Validated NT Sequence
gnncnctana tattttgttt actttaagaa ggagatatac catggctcac caccaccacc accatatggt gctgggatcg ccgatcgcgc actcccggtc tccgcaactg catctggccg cctaccgcgc gctcggcctg accggctgga cctacgaacg catcgaatgc gacgccgagc agctgcccgt gctcgtgggc ggtttcggcc cggaatgggt cggggtgtcg gtgacgatgc ccggcaagtt cgccgccctg acgttcgccg atcagcgcac cgcgcgcgcc gagctggtcg gatcggccaa caccctggtg cgccgggacg gcggctggct ggccgacaac accgacatcg acggtgtcaa gggcgcgctg ggcgccgtcg gtgagcatgt gatggtggtc ggttcgggcg gcaccgctcc cgcggcggtg gtcgcggtgg tcgagtcggg cgcccggcag gtgaccgtcg ccgcccgcaa tcgtgagaag gccgccccgt tggtggaact ggccgagcgg ctgggcgcct cggcccgttg gcaggacctc gccggggtca cggccgccgg ggtcgacgcg gtggtgagca cgattcccgc cgatgcggcc gcccggtacg cggcggcgct gtcggccacg ccggtgctgg tggacgcgat ctacgatccg tggccgacgc cgctggccgc ggccgtggcc gaagccggcg gtcgggtgat cagcgggctg gagatgctgc tgaatcaggc gttcacccag ggtcgagttg ttcaccggcc ggcccgcgcc caaagaagcg atgagggcgg cgctgaacca gccataagtg agtaagatag gatccggctg tnanancccc ccccccanan nnnnag
Expected Protein Sequence
MAHHHHHHML GSPIAHSRSP QLHLAAYRAL GLTGWTYERI ECDAEQLPVL VGGFGPEWVG VSVTMPGKFA ALTFADQRTA RAELVGSANT LVRRDGGWLA DNTDIDGVKG ALGAVGEHVM VVGSGGTAPA AVVAVVESGA RQVTVAARNR EKAAPLVELA ERLGASARWQ DLAGVTAAGV DAVVSTIPAD AAARYAAALS ATPVLVDAIY DPWPTPLAAA VAEAGGRVIS GLEMLLNQAF TQVELFTGRP APKEAMRAAL NQP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctgg gatcgccgat cgcgcactcc cggtctccgc aactgcatct ggccgcctac cgcgcgctcg gcctgaccgg ctggacctac gaacgcatcg aatgcgacgc cgagcagctg cccgtgctcg tgggcggttt cggcccggaa tgggtcgggg tgtcggtgac gatgcccggc aagttcgccg ccctgacgtt cgccgatcag cgcaccgcgc gcgccgagct ggtcggatcg gccaacaccc tggtgcgccg ggacggcggc tggctggccg acaacaccga catcgacggt gtcaagggcg cgctgggcgc cgtcggtgag catgtgatgg tggtcggttc gggcggcacc gctcccgcgg cggtggtcgc ggtggtcgag tcgggcgccc ggcaggtgac cgtcgccgcc cgcaatcgtg agaaggccgc cccgttggtg gaactggccg agcggctggg cgcctcggcc cgttggcagg acctcgccgg ggtcacggcc gccggggtcg acgcggtggt gagcacgatt cccgccgatg cggccgcccg gtacgcggcg gcgctgtcgg ccacgccggt gctggtggac gcgatctacg atccgtggcc gacgccgctg gccgcggccg tggccgaagc cggcggtcgg gtgatcagcg ggctggagat gctgctgaat caggcgttca cccaggtcga gttgttcacc ggccggcccg cgcccaaaga agcgatgagg gcggcgctga accagccatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat