MyfoA.00999.b
Shikimate 5-dehydrogenase (EC 1.1.1.25) (Rv2552c ortholog)
| CENTER ID: | MyfoA.00999.b |
| ORGANISM: | Mycobacterium fortuitum ATCC 6841 / DSM 46621 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyfoA.00999.b.B1.GE37816 | full length | 1 | 266 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4XIJ
DEPOSITED: 1/7/2015
DETERMINATION: XRay
CLONE: MyfoA.00999.b.B1.GE37816
PROTEIN: MyfoA.00999.b.B1.PS02069
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1214102.3.peg.5969 |
| RefSeq: | WP_003880746.1 |
| UniProt: | K0V1M6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDSRKAAVL GSPITHSRSP QLHLAAYRAL GLPSWTYERI ECTAEQLPGL VSALGPEWVG LSVTMPGKFA ALEFADQRTD RAQLVGSANT LVRMPTGGWR ADNTDVDGVT GALGTAGDSA LVIGSGGTAP AAVVALAELG VQRITIVARD EGKASRLVDL ARRCGAQGGW CDIGGAALAD AVASADAAVS TIPADAAAVY ADALARVPRV LDAIYDPWPT PLAQAVEAAG GEAINGLQML LNQAFAQVEQ FTGMPAPKEA MRAALG
NT Sequence
gtgcccgata gccgcaaagc ggccgtactc ggctcgccga tcacccactc gcgctcgcct cagctgcacc tggccgccta ccgggcgctc gggctgccgt cgtggaccta cgagcgcatc gagtgcacgg ccgaacaact gcccggcctc gtcagtgcac tgggcccgga atgggtcgga ttgtcggtca cgatgcccgg caagttcgcc gccctggaat tcgccgatca acgcaccgac cgtgcccagt tggtgggctc ggccaacacc ctggtccgga tgccgaccgg aggctggcgc gccgacaaca ccgacgtcga cggggtgacc ggagcactcg gcacggccgg cgactcggcg ctggtgatcg ggtccggcgg caccgccccg gcggccgtgg tggcactggc cgaactcggc gtgcagcgga tcaccatcgt ggcgcgcgac gagggcaagg catctcggtt ggtggacctg gcccgccggt gcggggcgca gggcggctgg tgcgacatcg gtggcgctgc gctggccgac gcggtggctt ccgcagatgc cgccgtgagc acgatcccgg ccgacgcggc cgccgtttac gcggacgcgc tggctcgggt gccgcgtgta ctggacgcca tctacgaccc gtggcccacg ccactggccc aggctgtcga ggccgcgggc ggcgaggcga tcaacgggct gcagatgttg ctgaaccagg cctttgccca ggtcgagcag ttcaccggga tgcccgcccc caaagaggcg atgcgcgccg ccctgggctg a
Details for MyfoA.00999.b.B1.GE37816
| HARVESTED ON: | 3/6/2014 |
| SEQUENCED ON: | 3/13/2014 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV PDSRKAAVLG SPITHSRSPQ LHLAAYRALG LPSWTYERIE CTAEQLPGLV SALGPEWVGL SVTMPGKFAA LEFADQRTDR AQLVGSANTL VRMPTGGWRA DNTDVDGVTG ALGTAGDSAL VIGSGGTAPA AVVALAELGV QRITIVARDE GKASRLVDLA RRCGAQGGWC DIGGAALADA VASADAAVST IPADAAAVYA DALARVPRVL DAIYDPWPTP LAQAVEAAGG EAINGLQMLL NQAFAQVEQF TGMPAPKEAM RAALG
Validated NT Sequence
atcttactca cttagcccag ggcggcgcgc atcgcctctt tgggggcggg catcccggtg aactgctcga cctgggcaaa ggcctggttc agcaacatct gcagcccgtt gatcgcctcg ccgcccgcgg cctcgacagc ctgggccagt ggcgtgggcc acgggtcgta gatggcgtcc agtacacgcg gcacccgagc cagcgcgtcc gcgtaaacgg cggccgcgtc ggccgggatc gtgctcacgg cggcatctgc ggaagccacc gcgtcggcca gcgcagcgcc accgatgtcg caccagccgc cctgcgcccc gcaccggcgg gccaggtcca ccaaccgaga tgccttgccc tcgtcgcgcg ccacgatggt gatccgctgc acgccgagtt cggccagtgc caccacggcc gccggggcgg tgccgccgga cccgatcacc agcgccgagt cgccggccgt gccgagtgct ccggtcaccc cgtcgacgtc ggtgttgtcg gcgcgccagc ctccggtcgg catccggacc agggtgttgg ccgagcccac caactgggca cggtcggtgc gttgatcggc gaattccagg gcggcgaact tgccgggcat cgtgaccgac aatccgaccc attccgggcc cagtgcactg acgaggccgg gcagttgttc ggccgtgcac tcgatgcgct cgtaggtcca cgacggcagc ccgagcgccc ggtaggcggc caggtgcagc tgaggcgagc gcgagtgggt gatcggcgag ccgagtacgg ccgctttgcg gctatcgggc accatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMP DSRKAAVLGS PITHSRSPQL HLAAYRALGL PSWTYERIEC TAEQLPGLVS ALGPEWVGLS VTMPGKFAAL EFADQRTDRA QLVGSANTLV RMPTGGWRAD NTDVDGVTGA LGTAGDSALV IGSGGTAPAA VVALAELGVQ RITIVARDEG KASRLVDLAR RCGAQGGWCD IGGAALADAV ASADAAVSTI PADAAAVYAD ALARVPRVLD AIYDPWPTPL AQAVEAAGGE AINGLQMLLN QAFAQVEQFT GMPAPKEAMR AALG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcccg atagccgcaa agcggccgta ctcggctcgc cgatcaccca ctcgcgctcg cctcagctgc acctggccgc ctaccgggcg ctcgggctgc cgtcgtggac ctacgagcgc atcgagtgca cggccgaaca actgcccggc ctcgtcagtg cactgggccc ggaatgggtc ggattgtcgg tcacgatgcc cggcaagttc gccgccctgg aattcgccga tcaacgcacc gaccgtgccc agttggtggg ctcggccaac accctggtcc ggatgccgac cggaggctgg cgcgccgaca acaccgacgt cgacggggtg accggagcac tcggcacggc cggcgactcg gcgctggtga tcgggtccgg cggcaccgcc ccggcggccg tggtggcact ggccgaactc ggcgtgcagc ggatcaccat cgtggcgcgc gacgagggca aggcatctcg gttggtggac ctggcccgcc ggtgcggggc gcagggcggc tggtgcgaca tcggtggcgc tgcgctggcc gacgcggtgg cttccgcaga tgccgccgtg agcacgatcc cggccgacgc ggccgccgtt tacgcggacg cgctggctcg ggtgccgcgt gtactggacg ccatctacga cccgtggccc acgccactgg cccaggctgt cgaggccgcg ggcggcgagg cgatcaacgg gctgcagatg ttgctgaacc aggcctttgc ccaggtcgag cagttcaccg ggatgcccgc ccccaaagag gcgatgcgcg ccgccctggg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat