MypaA.00999.a

AroE (Rv2552c ortholog)

CENTER ID: MypaA.00999.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00999.a.B1.GE37474 full length 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.1137
RefSeq: NP_960014.1
UniProt: Q741L0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSTAPPASA GPRRAAVLGK PIGHSKSPQL HLAAYRALGL HDWTYERIEC DADQLPGVVG GFGPEWVGVS VTMPGKFAAL RFADERTEQA RRVGSANTLV RTATGWRADN TDIDGVAGAL GAASGWALVC GSGGTAPAAV AGLAQLGVAG ITVVARNPDK AARLVRLGDE LGVPTRFCAL DGAGLADEVA AAEVLVSTLP AEVAERYAGT LARVGVLLDA VYDPWPTPLA AAVSAAGGRV ISGMQMLLHQ AFAQVEQFTG LPAPREAMTC AAADAD
NT Sequence
gtgtcctcga cagcaccccc cgctagcgcg gggccccgca gggcggccgt gctgggcaag ccgatcggcc actcgaaatc cccgcagctg cacctggccg cctaccgtgc gctgggcctg cacgactgga cctacgagcg catcgaatgc gacgccgacc agctgcccgg cgtggtcggc gggttcggcc ccgagtgggt cggggtgtcg gtgaccatgc ccggcaaatt cgcggcgctg cgcttcgccg acgagcgcac cgagcaggcg cggcgggtgg gatcggccaa caccctggtg cgcaccgcga cgggctggcg ggccgacaac accgacatcg acggcgtcgc cggcgcgctg ggcgccgctt cgggatgggc gctggtctgt ggatcgggcg gcaccgcgcc ggcggccgtc gcgggcctgg cccagctggg cgtcgccggc atcaccgtgg tggcccgcaa ccccgacaag gcggcccggc tggtgcgcct cggcgacgag ctgggcgtgc cgacccggtt ctgcgccctg gacggcgctg ggctggccga cgaggtggcc gcggccgagg tgctggtcag caccctgccg gcggaggtgg ccgaacgcta tgccggcacc ttggcgcgcg tcggcgtgct gctcgacgcc gtctacgacc cctggcccac cccgctggcc gccgcggtga gcgccgccgg cggccgggtg atcagcggca tgcagatgct gctgcaccag gccttcgccc aggtggaaca gttcaccggg ctgcccgccc cgcgcgaagc gatgacttgc gcggcggccg acgcggatta g
Details for MypaA.00999.a.B1.GE37474
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 29kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 67
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMV SSTAPPASAG PRXXAVLGKP IGHSKSPXLH LAAYRALGLH XWTYXRIXXX AXQLPGVVGG FGPEWVGVSV TMPGKFAALX FXDERTXQAR RXGSANTLVR TATGWRADNT DIXGXXXALG AXSGWALVCG SGGTAPAAVA GLXXXXXXGX TVVAXNPXXA ARLVRLXXXX GVPTXFCXXX GXXLXXXVAA PXCWSXXCXX XWPNAXXXXX XXXXXXXXXY XXXPPXWXXR
Validated NT Sequence
attttntttn nctttaagaa ggagatatac catggctcac caccaccacc accatatggt gtcctcgaca gcaccccccg ctagcgcggg gccccgcang gnggccgtgc tgggcaagcc gatcggccac tcgaaatccc cgcanctgca cctggccgcc taccgtgcgc tgggcctgca cnactggacc tacnagcgca tcnaangcna cgccnaccag ctgcccggcg tggtcggcgg gttcggcccc gagtgggtcg gggtgtcggt gaccatgccc ggcaaattcg cggcgctgcn cttcnccgac gagcgcaccn agcaggcgcg gcgggnggga tcggccaaca ccctggtgcg caccgcgacn ggntggcggg ccgacaacac cgacatcnac ggcntcnccn gcgcgctggg cgccncttcn ggatgggcgc tggtctgtgg atcgggcggc accgcgccgg cggccgtcgc gggcctggnc canntggncn tcnccggcnt caccgtggtg gcccncaacc ccnacnaggc ggcccggctg gtgcgcctcg ncnacnannt gggngtgccn acccngttct gcgncntgga nggnnnnngg ctggnnnacn angtggccgc gccnanntgc tggtcagnan cntgccngcn gangtggccn aacgctatnn gcncnnnnnn ncnngnnnnn ntgntnntcn acnccntcta cnannncgnn ccacccnnnt ggncgncgcg gtgannnnnn nnagcngnnn ggtgatnnnn nnntgcnnat nnnnntgcnn catgncttnn nccnngtgga acanttcacn nngctnnnnc nnnnnncnaa nngatgactt gcgcgnnngc cgacncgntn nnntnagtnn nataggatnc nnntgctnan nnannncgan ngnanctnan ttngnnnntg nnnnnnnnnn nnannnannn nnaannnaac cncngggncn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa a
Expected Protein Sequence
MAHHHHHHMS STAPPASAGP RRAAVLGKPI GHSKSPQLHL AAYRALGLHD WTYERIECDA DQLPGVVGGF GPEWVGVSVT MPGKFAALRF ADERTEQARR VGSANTLVRT ATGWRADNTD IDGVAGALGA ASGWALVCGS GGTAPAAVAG LAQLGVAGIT VVARNPDKAA RLVRLGDELG VPTRFCALDG AGLADEVAAA EVLVSTLPAE VAERYAGTLA RVGVLLDAVY DPWPTPLAAA VSAAGGRVIS GMQMLLHQAF AQVEQFTGLP APREAMTCAA ADAD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcct cgacagcacc ccccgctagc gcggggcccc gcagggcggc cgtgctgggc aagccgatcg gccactcgaa atccccgcag ctgcacctgg ccgcctaccg tgcgctgggc ctgcacgact ggacctacga gcgcatcgaa tgcgacgccg accagctgcc cggcgtggtc ggcgggttcg gccccgagtg ggtcggggtg tcggtgacca tgcccggcaa attcgcggcg ctgcgcttcg ccgacgagcg caccgagcag gcgcggcggg tgggatcggc caacaccctg gtgcgcaccg cgacgggctg gcgggccgac aacaccgaca tcgacggcgt cgccggcgcg ctgggcgccg cttcgggatg ggcgctggtc tgtggatcgg gcggcaccgc gccggcggcc gtcgcgggcc tggcccagct gggcgtcgcc ggcatcaccg tggtggcccg caaccccgac aaggcggccc ggctggtgcg cctcggcgac gagctgggcg tgccgacccg gttctgcgcc ctggacggcg ctgggctggc cgacgaggtg gccgcggccg aggtgctggt cagcaccctg ccggcggagg tggccgaacg ctatgccggc accttggcgc gcgtcggcgt gctgctcgac gccgtctacg acccctggcc caccccgctg gccgccgcgg tgagcgccgc cggcggccgg gtgatcagcg gcatgcagat gctgctgcac caggccttcg cccaggtgga acagttcacc gggctgcccg ccccgcgcga agcgatgact tgcgcggcgg ccgacgcgga ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat