MysmA.00358.i
Enoyl-CoA hydratase
| CENTER ID: | MysmA.00358.i |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00358.i.A1.GE28620 | Full length( MysmA.00358.i ) | 1 | 312 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00358.i.A1.PS00563 | Full length( MysmA.00358.i ) | 1 | 312 |
Structures
3NJB
DEPOSITED: 6/17/2010
DETERMINATION: XRay
CLONE: MysmA.00358.i.A1.GE28620
PROTEIN: MysmA.00358.i.A1.PS00563
3NJD
DEPOSITED: 6/17/2010
DETERMINATION: XRay
CLONE: MysmA.00358.i.A1.GE28620
PROTEIN: MysmA.00358.i.A1.PS00563
Publications by SSGCID
SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Christensen JS, Edwards TE, Gardberg AS, Myler PJ, Robinson JI, Staker BL, Stewart LJ
J. Struct. Funct. Genomics - 2011
volume 12, issue 2, pages 83-95
PMID: 21359836; PMCID: PMC3123459
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_211119 |
| PATRIC ID: | fig|246196.19.peg.1371 |
| RefSeq: | YP_885774.1 |
| UniProt: | A0QS86 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTHAIRPVDF DNLKTMTYEV TDRVARITFN RPEKGNAIVA DTPLELSALV ERADLDPDVH VILVSGRGEG FCAGFDLSAY AEGSSSAGGG SPYEGTVLSG KTQALNHLPD EPWDPMVDYQ MMSRFVRGFA SLMHCDKPTV VKIHGYCVAG GTDIALHADQ VIAAADAKIG YPPMRVWGVP AAGLWAHRLG DQRAKRLLFT GDCITGAQAA EWGLAVEAPD PADLDARTER LVERIAAMPV NQLIMAKLAC NTALLNQGVA TSQMVSTVFD GIARHTPEGH AFVATAREHG FREAVRRRDE PMGDHGRRAS DV
NT Sequence
ATGACGCACG CGATCCGGCC GGTCGACTTC GACAACCTCA AGACCATGAC CTACGAGGTC ACGGACCGGG TCGCGCGCAT CACGTTCAAC CGGCCCGAGA AGGGCAACGC GATCGTCGCG GACACGCCCC TGGAGTTGTC GGCGCTCGTC GAACGGGCCG ACCTTGACCC GGACGTCCAC GTCATCCTGG TCTCCGGGCG CGGTGAGGGG TTCTGCGCCG GATTCGACCT GAGCGCGTAC GCGGAAGGCT CGTCGTCTGC CGGTGGCGGC AGCCCGTACG AGGGCACCGT GCTGTCGGGA AAGACACAGG CGCTCAACCA CCTTCCGGAT GAGCCGTGGG ATCCGATGGT CGACTATCAG ATGATGAGCC GATTCGTTCG CGGCTTCGCG AGCCTGATGC ACTGTGACAA GCCCACCGTG GTGAAGATCC ACGGCTACTG CGTGGCCGGC GGCACCGACA TCGCGCTGCA CGCCGATCAG GTGATCGCCG CGGCCGACGC CAAGATCGGC TACCCGCCGA TGCGGGTGTG GGGCGTACCG GCCGCAGGGT TGTGGGCGCA CCGGCTGGGG GACCAGCGTG CGAAACGCCT GCTGTTCACC GGGGATTGCA TCACCGGGGC GCAGGCCGCC GAGTGGGGAC TGGCGGTCGA GGCGCCCGAC CCGGCCGATC TCGACGCGCG CACCGAGCGC CTCGTCGAAC GCATCGCGGC CATGCCCGTC AACCAATTGA TCATGGCCAA GCTCGCATGC AACACGGCGC TGCTCAACCA GGGCGTGGCG ACCAGCCAGA TGGTCAGCAC CGTCTTCGAC GGCATCGCCC GGCACACGCC GGAAGGACAT GCGTTCGTCG CGACGGCCCG CGAGCACGGG TTCCGCGAGG CCGTGCGTCG TCGCGACGAG CCGATGGGTG ACCACGGACG CAGGGCTTCG GACGTG
Details for MysmA.00358.i.A1.GE28620
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTHAIRPVD FDNLKTMTYE VTDRVARITF NRPEKGNAIV ADTPLELSAL VERADLDPDV HVILVSGRGE GFCAGFDLSA YAEGSSSAGG GSPYEGTVLS GKTQALNHLP DEPWDPMVDY QMMSRFVRGF ASLMHCDKPT VVKIHGYCVA GGTDIALHAD QVIAAADAKI GYPPMRVWGV PAAGLWAHRL GDQRAKRLLF TGDCITGAQA AEWGLAVEAP DPADLDARTE RLVERIAAMP VNQLIMAKLA CNTALLNQGV ATSQMVSTVF DGXARHTPEG XAFVATAREH GXREAVRR
Validated NT Sequence
ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgacg cacgcgatcc ggccggtcga cttcgacaac ctcaagacca tgacctacga ggtcacggac cgggtcgcgc gcatcacgtt caaccggccc gagaagggca acgcgatcgt cgcggacacg cccctggagt tgtcggcgct cgtcgaacgg gccgaccttg acccggacgt ccacgtcatc ctggtctccg ggcgcggtga ggggttctgc gccggattcg acctgagcgc gtacgcggaa ggctcgtcgt ctgccggtgg cggcagcccg tacgagggca ccgtgctgtc gggaaagaca caggcgctca accaccttcc ggatgagccg tgggatccga tggtcgacta tcagatgatg agccgattcg ttcgcggctt cgcgagcctg atgcactgtg acaagcccac cgtggtgaag atccacggct actgcgtggc cggcggcacc gacatcgcgc tgcacgccga tcaggtgatc gccgcggccg acgccaagat cggctacccg ccgatgcggg tgtggggcgt accggccgca gggttgtggg cgcaccggct gggggaccag cgtgcgaaac gcctgctgtt caccggggat tgcatcaccg gggcgcaggc cgccgagtgg ggactggcgg tcgaggcgcc cgacccggcc gatctcgacg cgcgcaccga gcgcctcgtc gaacgcatcg cggccatgcc cgtcaaccaa ttgatcatgg ccaagctcgc atgcaacacg gcgctgctca accagggcgt ggcgaccagc cagatggtca gcaccgtctt cgacggcntc gcccggcaca cgccggaagg acntgcgttc gtcgcgacgg cccgcgagca cggnntccgc gaggccgtgc gtcgnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTHAIRPVD FDNLKTMTYE VTDRVARITF NRPEKGNAIV ADTPLELSAL VERADLDPDV HVILVSGRGE GFCAGFDLSA YAEGSSSAGG GSPYEGTVLS GKTQALNHLP DEPWDPMVDY QMMSRFVRGF ASLMHCDKPT VVKIHGYCVA GGTDIALHAD QVIAAADAKI GYPPMRVWGV PAAGLWAHRL GDQRAKRLLF TGDCITGAQA AEWGLAVEAP DPADLDARTE RLVERIAAMP VNQLIMAKLA CNTALLNQGV ATSQMVSTVF DGIARHTPEG HAFVATAREH GFREAVRRRD EPMGDHGRRA SDV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcacgcg atccggccgg tcgacttcga caacctcaag accatgacct acgaggtcac ggaccgggtc gcgcgcatca cgttcaaccg gcccgagaag ggcaacgcga tcgtcgcgga cacgcccctg gagttgtcgg cgctcgtcga acgggccgac cttgacccgg acgtccacgt catcctggtc tccgggcgcg gtgaggggtt ctgcgccgga ttcgacctga gcgcgtacgc ggaaggctcg tcgtctgccg gtggcggcag cccgtacgag ggcaccgtgc tgtcgggaaa gacacaggcg ctcaaccacc ttccggatga gccgtgggat ccgatggtcg actatcagat gatgagccga ttcgttcgcg gcttcgcgag cctgatgcac tgtgacaagc ccaccgtggt gaagatccac ggctactgcg tggccggcgg caccgacatc gcgctgcacg ccgatcaggt gatcgccgcg gccgacgcca agatcggcta cccgccgatg cgggtgtggg gcgtaccggc cgcagggttg tgggcgcacc ggctggggga ccagcgtgcg aaacgcctgc tgttcaccgg ggattgcatc accggggcgc aggccgccga gtggggactg gcggtcgagg cgcccgaccc ggccgatctc gacgcgcgca ccgagcgcct cgtcgaacgc atcgcggcca tgcccgtcaa ccaattgatc atggccaagc tcgcatgcaa cacggcgctg ctcaaccagg gcgtggcgac cagccagatg gtcagcaccg tcttcgacgg catcgcccgg cacacgccgg aaggacatgc gttcgtcgcg acggcccgcg agcacgggtt ccgcgaggcc gtgcgtcgtc gcgacgagcc gatgggtgac cacggacgca gggcttcgga cgtgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MysmA.00358.i.A1.PS00563
| PURIFICATION DATe: | 4/13/2010 |
| CONCENTRATION: | 31.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTHAIRPVD FDNLKTMTYE VTDRVARITF NRPEKGNAIV ADTPLELSAL VERADLDPDV HVILVSGRGE GFCAGFDLSA YAEGSSSAGG GSPYEGTVLS GKTQALNHLP DEPWDPMVDY QMMSRFVRGF ASLMHCDKPT VVKIHGYCVA GGTDIALHAD QVIAAADAKI GYPPMRVWGV PAAGLWAHRL GDQRAKRLLF TGDCITGAQA AEWGLAVEAP DPADLDARTE RLVERIAAMP VNQLIMAKLA CNTALLNQGV ATSQMVSTVF DGXARHTPEG XAFVATAREH GXREAVRR
Validated NT Sequence
ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgacg cacgcgatcc ggccggtcga cttcgacaac ctcaagacca tgacctacga ggtcacggac cgggtcgcgc gcatcacgtt caaccggccc gagaagggca acgcgatcgt cgcggacacg cccctggagt tgtcggcgct cgtcgaacgg gccgaccttg acccggacgt ccacgtcatc ctggtctccg ggcgcggtga ggggttctgc gccggattcg acctgagcgc gtacgcggaa ggctcgtcgt ctgccggtgg cggcagcccg tacgagggca ccgtgctgtc gggaaagaca caggcgctca accaccttcc ggatgagccg tgggatccga tggtcgacta tcagatgatg agccgattcg ttcgcggctt cgcgagcctg atgcactgtg acaagcccac cgtggtgaag atccacggct actgcgtggc cggcggcacc gacatcgcgc tgcacgccga tcaggtgatc gccgcggccg acgccaagat cggctacccg ccgatgcggg tgtggggcgt accggccgca gggttgtggg cgcaccggct gggggaccag cgtgcgaaac gcctgctgtt caccggggat tgcatcaccg gggcgcaggc cgccgagtgg ggactggcgg tcgaggcgcc cgacccggcc gatctcgacg cgcgcaccga gcgcctcgtc gaacgcatcg cggccatgcc cgtcaaccaa ttgatcatgg ccaagctcgc atgcaacacg gcgctgctca accagggcgt ggcgaccagc cagatggtca gcaccgtctt cgacggcntc gcccggcaca cgccggaagg acntgcgttc gtcgcgacgg cccgcgagca cggnntccgc gaggccgtgc gtcgnc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTHAIRPVD FDNLKTMTYE VTDRVARITF NRPEKGNAIV ADTPLELSAL VERADLDPDV HVILVSGRGE GFCAGFDLSA YAEGSSSAGG GSPYEGTVLS GKTQALNHLP DEPWDPMVDY QMMSRFVRGF ASLMHCDKPT VVKIHGYCVA GGTDIALHAD QVIAAADAKI GYPPMRVWGV PAAGLWAHRL GDQRAKRLLF TGDCITGAQA AEWGLAVEAP DPADLDARTE RLVERIAAMP VNQLIMAKLA CNTALLNQGV ATSQMVSTVF DGIARHTPEG HAFVATAREH GFREAVRRRD EPMGDHGRRA SDV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGCACGCG ATCCGGCCGG TCGACTTCGA CAACCTCAAG ACCATGACCT ACGAGGTCAC GGACCGGGTC GCGCGCATCA CGTTCAACCG GCCCGAGAAG GGCAACGCGA TCGTCGCGGA CACGCCCCTG GAGTTGTCGG CGCTCGTCGA ACGGGCCGAC CTTGACCCGG ACGTCCACGT CATCCTGGTC TCCGGGCGCG GTGAGGGGTT CTGCGCCGGA TTCGACCTGA GCGCGTACGC GGAAGGCTCG TCGTCTGCCG GTGGCGGCAG CCCGTACGAG GGCACCGTGC TGTCGGGAAA GACACAGGCG CTCAACCACC TTCCGGATGA GCCGTGGGAT CCGATGGTCG ACTATCAGAT GATGAGCCGA TTCGTTCGCG GCTTCGCGAG CCTGATGCAC TGTGACAAGC CCACCGTGGT GAAGATCCAC GGCTACTGCG TGGCCGGCGG CACCGACATC GCGCTGCACG CCGATCAGGT GATCGCCGCG GCCGACGCCA AGATCGGCTA CCCGCCGATG CGGGTGTGGG GCGTACCGGC CGCAGGGTTG TGGGCGCACC GGCTGGGGGA CCAGCGTGCG AAACGCCTGC TGTTCACCGG GGATTGCATC ACCGGGGCGC AGGCCGCCGA GTGGGGACTG GCGGTCGAGG CGCCCGACCC GGCCGATCTC GACGCGCGCA CCGAGCGCCT CGTCGAACGC ATCGCGGCCA TGCCCGTCAA CCAATTGATC ATGGCCAAGC TCGCATGCAA CACGGCGCTG CTCAACCAGG GCGTGGCGAC CAGCCAGATG GTCAGCACCG TCTTCGACGG CATCGCCCGG CACACGCCGG AAGGACATGC GTTCGTCGCG ACGGCCCGCG AGCACGGGTT CCGCGAGGCC GTGCGTCGTC GCGACGAGCC GATGGGTGAC CACGGACGCA GGGCTTCGGA CGTGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA