MyavA.00358.e

Enoyl-CoA hydratase/isomerase family protein

CENTER ID: MyavA.00358.e
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00358.e.A1.GE28522 Full length( MyavA.00358.e ) 1 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1547
RefSeq: YP_880819.1
UniProt: A0A0H2ZVN0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTASSTSDDR VLFDVDAEHR IATITLNNAK QRNSYDAAMR EAIARCLDRV AEDDDLTVVL LRGAEGVFST GADMNNAYGW YGTSRNADES TRRRPSQRRR LTVDRKSFGF YHNFMGFPKV TVGEIAGYAL GGGFEMALMT DISVIARDTK IGMPATRFLG PALGSLHMFF HRLGPVLARR LLLTGDIIDA GQVEHLGIFT DTCDPAAVTA RARYWAEKAA KMPADGVVIA KEAFRLVEQS QAYQGEEVAS YLFHAFGTNL QFAPGEFNFV KTRAEHGAKE AFRLRDEHFH VPEPEA
NT Sequence
ATGACCGCCT CGAGCACCTC CGACGATCGC GTGCTCTTCG ACGTCGACGC CGAGCACCGC ATCGCGACGA TCACGCTGAA CAATGCCAAG CAGCGCAACT CCTACGACGC CGCCATGCGC GAGGCCATCG CCCGCTGCCT GGATCGCGTC GCCGAGGACG ACGACCTCAC CGTCGTGCTG CTGCGCGGGG CCGAGGGGGT CTTTTCCACC GGGGCCGACA TGAACAACGC CTACGGCTGG TACGGCACGT CACGCAACGC GGACGAGTCC ACCCGGCGCC GCCCCAGCCA GCGTCGGCGA CTCACGGTGG ACCGCAAGTC TTTTGGCTTC TACCACAATT TCATGGGCTT CCCGAAGGTG ACGGTCGGCG AGATCGCCGG CTACGCGCTC GGCGGCGGCT TCGAGATGGC GCTGATGACC GACATCTCGG TGATCGCGCG GGACACCAAG ATCGGCATGC CGGCCACCCG CTTCCTGGGC CCCGCGCTCG GCAGCCTGCA CATGTTCTTC CACCGGCTGG GGCCGGTGCT GGCTCGGCGC CTACTGCTGA CCGGCGACAT CATCGACGCC GGGCAGGTCG AACACCTCGG AATCTTCACC GACACCTGCG ATCCCGCTGC GGTGACGGCC CGGGCGCGCT ACTGGGCCGA GAAGGCGGCG AAGATGCCCG CCGACGGGGT GGTGATCGCC AAGGAGGCCT TCCGGCTGGT CGAGCAGAGC CAGGCGTATC AGGGCGAGGA GGTCGCCAGC TATCTGTTCC ATGCCTTCGG CACCAACCTG CAATTCGCCC CGGGGGAGTT CAACTTCGTC AAGACCCGCG CCGAGCACGG CGCCAAGGAA GCGTTCCGGC TTCGCGACGA GCACTTCCAC GTGCCGGAAC CCGAGGCG
Details for MyavA.00358.e.A1.GE28522
HARVESTED ON: 2/9/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTASSTSDD RVLFDVDAEH RIATITLNNA KQRNSYDAAM REAIARCLDR VAEDDDLTVV LLRGAEGVFS TGADMNNAYG WYGTSRNADE STRRRPSQRR RLTVDRKSFG FYHNFMGFPK VTVGEIAGYA LGGGFEMALM TDISVIARDT KIGMPATRFL GPALGSLHMF FHRLGPVLAR RLLLTGDIID AGQVEHLGIF TDTCDPAAVT ARARYWAEKA AKMPADGVVI AKEAFRLVEQ SQAYQGEEVA SYLFHAFGTN LQFAPGEFNF VKTRAEHGAK EAFRLRDEHF HVPEP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg cctcgagcac ctccgacgat cgcgtgctct tcgacgtcga cgccgagcac cgcatcgcga cgatcacgct gaacaatgcc aagcagcgca actcctacga cgccgccatg cgcgaggcca tcgcccgctg cctggatcgc gtcgccgagg acgacgacct caccgtcgtg ctgctgcgcg gggccgaggg ggtcttttcc accggggccg acatgaacaa cgcctacggc tggtacggca cgtcacgcaa cgcggacgag tccacccggc gccgccccag ccagcgtcgg cgactcacgg tggaccgcaa gtcttttggc ttctaccaca atttcatggg cttcccgaag gtgacggtcg gcgagatcgc cggctacgcg ctcggcggcg gcttcgagat ggcgctgatg accgacatct cggtgatcgc gcgggacacc aagatcggca tgccggccac ccgcttcctg ggccccgcgc tcggcagcct gcacatgttc ttccaccggc tggggccggt gctggctcgg cgcctactgc tgaccggcga catcatcgac gccgggcagg tcgaacacct cggaatcttc accgacacct gcgatcccgc tgcggtgacg gcccgggcgc gctactgggc cgagaaggcg gcgaagatgc ccgccgacgg ggtggtgatc gccaaggagg ccttccggct ggtcgagcag agccaggcgt atcagggcga ggaggtcgcc agctatctgt tccatgcctt cggcaccaac ctgcaattcg ccccggggga gttcaacttc gtcaagaccc gcgccgagca cggcgccaag gaagcgttcc ggcttcgcga cgagcacttc cacgtgccgg aacccg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTASSTSDD RVLFDVDAEH RIATITLNNA KQRNSYDAAM REAIARCLDR VAEDDDLTVV LLRGAEGVFS TGADMNNAYG WYGTSRNADE STRRRPSQRR RLTVDRKSFG FYHNFMGFPK VTVGEIAGYA LGGGFEMALM TDISVIARDT KIGMPATRFL GPALGSLHMF FHRLGPVLAR RLLLTGDIID AGQVEHLGIF TDTCDPAAVT ARARYWAEKA AKMPADGVVI AKEAFRLVEQ SQAYQGEEVA SYLFHAFGTN LQFAPGEFNF VKTRAEHGAK EAFRLRDEHF HVPEPEA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgcctcg agcacctccg acgatcgcgt gctcttcgac gtcgacgccg agcaccgcat cgcgacgatc acgctgaaca atgccaagca gcgcaactcc tacgacgccg ccatgcgcga ggccatcgcc cgctgcctgg atcgcgtcgc cgaggacgac gacctcaccg tcgtgctgct gcgcggggcc gagggggtct tttccaccgg ggccgacatg aacaacgcct acggctggta cggcacgtca cgcaacgcgg acgagtccac ccggcgccgc cccagccagc gtcggcgact cacggtggac cgcaagtctt ttggcttcta ccacaatttc atgggcttcc cgaaggtgac ggtcggcgag atcgccggct acgcgctcgg cggcggcttc gagatggcgc tgatgaccga catctcggtg atcgcgcggg acaccaagat cggcatgccg gccacccgct tcctgggccc cgcgctcggc agcctgcaca tgttcttcca ccggctgggg ccggtgctgg ctcggcgcct actgctgacc ggcgacatca tcgacgccgg gcaggtcgaa cacctcggaa tcttcaccga cacctgcgat cccgctgcgg tgacggcccg ggcgcgctac tgggccgaga aggcggcgaa gatgcccgcc gacggggtgg tgatcgccaa ggaggccttc cggctggtcg agcagagcca ggcgtatcag ggcgaggagg tcgccagcta tctgttccat gccttcggca ccaacctgca attcgccccg ggggagttca acttcgtcaa gacccgcgcc gagcacggcg ccaaggaagc gttccggctt cgcgacgagc acttccacgt gccggaaccc gaggcgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa