MyabA.00386.b

Possible enoyl-CoA hydratase/isomerase

CENTER ID: MyabA.00386.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00386.b.A1.GE30296 Full length( MyabA.00386.b ) 1 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3930
RefSeq: YP_001704587.1
UniProt: B1MGI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVDTLKTMTY EVTDRVARIT FNRPEQGNAI IADTPLELAA LVERADLDPQ VHVILVSGRG DGFCGGFDLS AYAEANDEGS VRYSGTALDG QVQLRNHLPN INWDPMLDYQ MMSRFTRGFS SLLHANKPTV VKVHGYCVAG GTDIALHADQ LIVAADAKIG YPPTRVWGVP ATGLWAHKLG DQRAKRLLLT GDCLSGRQAY DWGLAVEAPE PDELDERTER LVERIAAMPL NQLMMIKLAC NSVLINQGIA NSAMIGTVFD GISRHTPEAH AFTADAIANG YRQAVRHRDE PFGDYGRKPT EL
NT Sequence
ATGGTGGACA CCTTGAAGAC GATGACCTAC GAGGTCACCG ATCGAGTCGC GCGCATCACC TTCAACCGGC CCGAGCAGGG CAACGCCATC ATCGCCGACA CTCCGCTGGA ACTGGCGGCA CTCGTTGAAC GTGCCGATCT GGATCCTCAG GTGCACGTGA TCCTGGTATC CGGCCGGGGG GACGGGTTCT GTGGCGGCTT CGACCTCAGT GCCTATGCCG AGGCCAATGA CGAAGGGTCG GTGCGGTATT CAGGTACCGC GCTGGACGGG CAGGTGCAGC TGCGCAACCA CCTGCCCAAC ATCAATTGGG ATCCGATGCT CGACTATCAG ATGATGAGCC GGTTCACGCG CGGCTTCTCC TCGCTATTGC ATGCCAACAA GCCCACCGTG GTGAAGGTGC ACGGCTATTG CGTGGCGGGC GGAACGGATA TCGCATTACA CGCCGACCAG TTGATCGTCG CCGCTGATGC CAAGATCGGA TACCCGCCTA CGCGGGTGTG GGGTGTGCCC GCCACCGGAC TATGGGCGCA CAAGCTCGGT GATCAGCGTG CGAAACGCCT TCTACTGACT GGTGATTGCC TGTCGGGCAG GCAAGCCTAC GACTGGGGTC TGGCGGTCGA GGCACCCGAG CCCGACGAGT TGGACGAGCG CACCGAGCGG CTGGTCGAGC GGATCGCCGC GATGCCGTTG AACCAGCTGA TGATGATCAA GCTGGCCTGC AACTCTGTAC TCATCAATCA GGGGATTGCC AACAGCGCCA TGATCGGCAC AGTTTTTGAC GGTATCTCCC GGCATACACC GGAGGCTCAC GCGTTCACGG CAGACGCCAT TGCCAACGGC TATCGGCAAG CGGTCCGGCA CCGGGATGAG CCGTTTGGCG ATTACGGGCG TAAGCCCACC GAACTG
Details for MyabA.00386.b.A1.GE30296
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 86
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDTLKTMT YEVTDRVARI TFNRPEQGNA IIADTPLELA ALVERADLDP QVHVILVSGR GDGFCGGFDL SAYAEANDEG SVRYSGTALD GQVQLRNHLP NINWDPMLDY QMMSRFTRGF SSLLHANKPT VVKVHGYCVA GGTDIALHAD QLIVAADAKI GYPPTRVWGV PATGLWAHKL GDQRAKRLLL TGDCLSGRQA YDWGLAVEAP EPDELDERTE RLVERIAAMP LNQLMMIKLA CNSVLINXGI ANSAMIGTVF
Validated NT Sequence
tannnnngnn nnntttaaga aggagatata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggt ggacaccttg aagacgatga cctacgaggt caccgatcga gtcgcgcgca tcaccttcaa ccggcccgag cagggcaacg ccatcatcgc cgacactccg ctggaactgg cggcactcgt tgaacgtgcc gatctggatc ctcaggtgca cgtgatcctg gtatccggcc ggggggacgg gttctgtggc ggcttcgacc tcagtgccta tgccgaggcc aatgacgaag ggtcggtgcg gtattcaggt accgcgctgg acgggcaggt gcagctgcgc aaccacctgc ccaacatcaa ttgggatccg atgctcgact atcagatgat gagccggttc acgcgcggct tctcctcgct attgcatgcc aacaagccca ccgtggtgaa ggtgcacggc tattgcgtgg cgggcggaac ggatatcgca ttacacgccg accagttgat cgtcgccgct gatgccaaga tcggataccc gcctacgcgg gtgtggggtg tgcccgccac cggactatgg gcgcacaagc tcggtgatca gcgtgcgaaa cgccttctac tgactggtga ttgcctgtcg ggcaggcaag cctacgactg gggtctggcg gtcgaggcac ccgagcccga cgagttggac gagcgcaccg agcggctggt cgagcggatc gccgcgatgc cgttgaacca gctgatgatg atcaagctgg cctgcaactc tgtactcatc aatcnnggga ttgccaacag cgccatgatc ggcacagttt tttgacggat ctcccggcat acaccggagg ctcacgcgtt cacggcagac gccattgcaa cggctatcgg caagcggtcc ggcnccggnt gagccgtttg gcgattacng gngtnagccc accgaactgn aataaacagc acgaacnagt tctgcagccn ncttctcnan nnncngctgc taacnanccc naannnaanc tnantnnctg nngcnnnnnn nnnnnnnnnn nnnnnnnnnn nnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDTLKTMT YEVTDRVARI TFNRPEQGNA IIADTPLELA ALVERADLDP QVHVILVSGR GDGFCGGFDL SAYAEANDEG SVRYSGTALD GQVQLRNHLP NINWDPMLDY QMMSRFTRGF SSLLHANKPT VVKVHGYCVA GGTDIALHAD QLIVAADAKI GYPPTRVWGV PATGLWAHKL GDQRAKRLLL TGDCLSGRQA YDWGLAVEAP EPDELDERTE RLVERIAAMP LNQLMMIKLA CNSVLINQGI ANSAMIGTVF DGISRHTPEA HAFTADAIAN GYRQAVRHRD EPFGDYGRKP TEL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggacacc ttgaagacga tgacctacga ggtcaccgat cgagtcgcgc gcatcacctt caaccggccc gagcagggca acgccatcat cgccgacact ccgctggaac tggcggcact cgttgaacgt gccgatctgg atcctcaggt gcacgtgatc ctggtatccg gccgggggga cgggttctgt ggcggcttcg acctcagtgc ctatgccgag gccaatgacg aagggtcggt gcggtattca ggtaccgcgc tggacgggca ggtgcagctg cgcaaccacc tgcccaacat caattgggat ccgatgctcg actatcagat gatgagccgg ttcacgcgcg gcttctcctc gctattgcat gccaacaagc ccaccgtggt gaaggtgcac ggctattgcg tggcgggcgg aacggatatc gcattacacg ccgaccagtt gatcgtcgcc gctgatgcca agatcggata cccgcctacg cgggtgtggg gtgtgcccgc caccggacta tgggcgcaca agctcggtga tcagcgtgcg aaacgccttc tactgactgg tgattgcctg tcgggcaggc aagcctacga ctggggtctg gcggtcgagg cacccgagcc cgacgagttg gacgagcgca ccgagcggct ggtcgagcgg atcgccgcga tgccgttgaa ccagctgatg atgatcaagc tggcctgcaa ctctgtactc atcaatcagg ggattgccaa cagcgccatg atcggcacag tttttgacgg tatctcccgg catacaccgg aggctcacgc gttcacggca gacgccattg ccaacggcta tcggcaagcg gtccggcacc gggatgagcc gtttggcgat tacgggcgta agcccaccga actgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa