MyulA.18528.a

Bifunctional penicillin-binding protein 1A/1B PonA1 (Rv0050 ortholog)

CENTER ID: MyulA.18528.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.18528.a.B2.GE37678 mature protein 15 680
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.162
RefSeq: YP_904312.1
UniProt: A0PKH2

Orthologues for Rv0050

Species % Identity % Coverage Clones Proteins Structures
marinum 92 98 1 1 0
ulcerans 91 98 1 0 0
fortuitum 79 96 0 0 0
thermoresistibile 78 97 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVILLPIVTF TVAYFIVDVP RPGDIRTNQV STILASDGSE IAKIVPPEGN RVDVNLSQVP EHVRAAVIAA EDRGFYSNPG FSFSGFARAI KNNLFGGDLQ GGSTITQQYV KNALVGSAQH GWSGLMRKAK ELVIATKMSG EWSKDDVLQA YLNIIYFGRG AYGISAASKA YFDKPVEQLT VSEGALLAAL IRRPSTLDPA VDPDGALARW NWVLDGMVDT KALSAKDRAE QAFPRTVPPD QARAANQTTG PNGLIERQVT KELLELFNID EQTLNTQGLQ VTTTIDAQAQ QAAEKAVAKY LDGQDPEMRA AVVSIDPHNG AVRAYYGGDN ANGFDFAQAG LQTGSSFKVF ALVAALEQGI GLGYDVDSSP LTVDGIKITN VEGESCGTCN IAQALKMSLN TPYYRLMLKL KGGPEAVADA AHQAGIATSF PGVPHTLSED GKGGPPNNGI VLGQYQTRVI DMASAYATLA ASGIYHRPHF VQKVVDADGR VLFDASTEDN TGDQRIPKAV ADNVTAAMEP IAGYSRGHNL AGGRPSASKT GTVQLGDTSA NRDAWMVGYT PSLSTAVWVG TVKGDEPLVT ASGAPIYGSG LPSDIWKATM DGALKGTEVE SFPKPTEIGG YAGVPAPPPP PPEAPPPAET VIQPTVEIVP GITIPVGPPT TITLAPPPPP GAPPADNPPP
NT Sequence
gtggtcatcc tgctgcccat cgtcaccttc accgtggcct acttcatcgt cgatgtgcca aggccgggcg acatccgcac caatcaggtg tccacgatcc tggctagcga cggctccgag atcgccaaga tcgttccccc cgaaggcaat cgggtcgacg tcaacctcag tcaggtgccc gagcatgtcc gcgccgcggt catcgccgcc gaagaccgcg gcttctactc caacccgggg ttctccttca gcggcttcgc gcgagcgata aagaacaact tgttcggcgg tgacctgcaa ggcggctcca cgatcaccca gcaatacgtc aagaacgcgt tggtcggctc ggcgcaacac gggtggagcg gcctgatgcg caaggccaag gaactcgtca tcgccaccaa gatgtcgggg gagtggtcca aagacgatgt cctgcaggcc tatctcaaca tcatctactt cggccgcggc gcatacggga tttcggcagc gtccaaggcc tacttcgaca agccggtcga gcagctcacc gtgtcggagg gagccctgct ggcggcgttg attcggcggc cctccacgct ggacccggcg gtggatcccg atggggcgct ggcgcgctgg aactgggtgc tcgacggcat ggtggatacc aaggcgttgt ccgccaaaga ccgggccgag caggcatttc ccaggaccgt gccgccggat caagcccgcg cggccaacca gaccaccggg cccaacgggc tgatcgaacg ccaggtcacc aaagagttgt tggagctgtt caacatcgat gaacagaccc tgaacacgca ggggctgcag gtcacgacca cgatcgatgc ccaagcgcag caggcggccg aaaaggcggt agcgaaatac ctcgacgggc aggatcccga gatgcgggcc gcggtggtct ccatcgaccc gcacaacggg gccgtgcgcg cctattacgg cggcgacaac gccaacgggt tcgacttcgc ccaggccggg ctgcagacgg gatcctcgtt caaggtattc gccctggtgg ccgcccttga acagggaatt ggcctgggct atgacgtcga cagttcgcca ctgacggtgg acggcatcaa gatcaccaat gtcgaaggcg agagctgcgg tacctgcaac atcgcgcagg cgctcaagat gtcgctgaac accccctact accggctgat gctcaaactg aagggcggcc cggaggccgt cgccgacgcc gcgcaccagg ccggcatcgc caccagcttc ccgggcgtgc cccacacgct gtccgaggac ggcaagggcg gaccgcccaa caacgggatt gtgctgggcc agtatcagac ccgggtgatc gacatggctt cggcctacgc cacgctggcc gcatccggga tctatcaccg gccacatttc gtgcagaaag tcgtcgacgc cgacggccgg gttctcttcg acgcctccac cgaagacaac accggtgacc agcgcatccc caaggcggta gccgacaacg tcaccgcggc aatggaaccc attgccggat attcgcgtgg tcacaacctg gccggcggcc ggccgtcggc ctccaagacc ggcacggtgc agttgggcga caccagcgcc aaccgagacg cctggatggt cggttacacc ccgtcactgt cgacggccgt gtgggtggga acggtcaagg gtgacgagcc gttggtgacc gcctcgggcg caccgattta cggctcgggc ctgccgtcgg acatctggaa agccaccatg gacggtgcat tgaagggcac cgaggtcgag agcttcccca agcccaccga gatcggcggc tacgccggcg tgccggcacc gcctccacct ccaccggagg cgccgccgcc cgcggagacc gtcatccagc ccacggtcga gatagtgccc gggatcacga ttcccgtcgg gccgccaacc accatcactc ttgctccccc tccgccacca ggggcgccac ccgccgacaa tccgccaccg tga
Details for MyulA.18528.a.B2.GE37678
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/10/2014
EXPECTED MW: 71kDa
OBSERVED MW: 77kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 43
Validated AA Sequence
MAHHHHHHMF IVDVPRPGDI RTNQVSTILA SDGSEIAKIV PPEGNRVDVN LSQVPEHVRA AVIAAEDRGF YSNPGFSFSG FARAIKNNLF GGDLQGGSTI TQQYVKNALV GSAQHGWSGL MRKAKELVIA TKMSGEWSKD DVLQAYLNII YFGRGAYGIS AASKAYFDKP VEQLTVSEGA LLAALIRRPS TLDPAVDPDG ALARWNWVLD GMVDTKALSA KDRAEQAFPR TVPPDQARAA NQTTGPNGLI ERQVTKELLE LFNIDEQTLN TXGLQVTTTI DAQAQXXPKX X
Validated NT Sequence
atggctcacc accaccacca ccatatgttc atcgtcgatg tgccaaggcc gggcgacatc cgcaccaatc aggtgtccac gatcctggct agcgacggct ccgagatcgc caagatcgtt ccccccgaag gcaatcgggt cgacgtcaac ctcagtcagg tgcccgagca tgtccgcgcc gcggtcatcg ccgccgaaga ccgcggcttc tactccaacc cggggttctc cttcagcggc ttcgcgcgag cgataaagaa caacttgttc ggcggtgacc tgcaaggcgg ctccacgatc acccagcaat acgtcaagaa cgcgttggtc ggctcggcgc aacacgggtg gagcggcctg atgcgcaagg ccaaggaact cgtcatcgcc accaagatgt cgggggagtg gtccaaagac gatgtcctgc aggcctatct caacatcatc tacttcggcc gcggcgcata cgggatttcg gcagcgtcca aggcctactt cgacaagccg gtcgagcagc tcaccgtgtc ggagggagcc ctgctggcgg cgttgattcg gcggccctcc acgctggacc cggcggtgga tcccgatggg gcgctggcgc gctggaactg ggtgctcgac ggcatggtgg ataccaaggc gttgtccgcc aaagaccggg ccgagcaggc atttcccagg accgtgccgc cggatcaagc ccgcgcggcc aaccagacca ccgggcccaa cgggctgatc gaacgccagg tcaccaaaga gttgttggag ctgttcaaca tcgatgaaca gaccctgaac acgcangggc tgcaggtcac gaccacgatc gatgcccaag cgcagcnnng gccnaaaagn nggtagcgaa atacctcgac gggcagnatc cnnagangng ggccgcggtg gtctccatcg accnncacaa cggggccgtg cgcgcctatn nggnggcnan ncgccaacgg gttcnacttc nccnagncng gntgcnnang gnancntcnt tcnaggnnnt nnncctggnn ncnncccntn a
Expected Protein Sequence
MAHHHHHHFI VDVPRPGDIR TNQVSTILAS DGSEIAKIVP PEGNRVDVNL SQVPEHVRAA VIAAEDRGFY SNPGFSFSGF ARAIKNNLFG GDLQGGSTIT QQYVKNALVG SAQHGWSGLM RKAKELVIAT KMSGEWSKDD VLQAYLNIIY FGRGAYGISA ASKAYFDKPV EQLTVSEGAL LAALIRRPST LDPAVDPDGA LARWNWVLDG MVDTKALSAK DRAEQAFPRT VPPDQARAAN QTTGPNGLIE RQVTKELLEL FNIDEQTLNT QGLQVTTTID AQAQQAAEKA VAKYLDGQDP EMRAAVVSID PHNGAVRAYY GGDNANGFDF AQAGLQTGSS FKVFALVAAL EQGIGLGYDV DSSPLTVDGI KITNVEGESC GTCNIAQALK MSLNTPYYRL MLKLKGGPEA VADAAHQAGI ATSFPGVPHT LSEDGKGGPP NNGIVLGQYQ TRVIDMASAY ATLAASGIYH RPHFVQKVVD ADGRVLFDAS TEDNTGDQRI PKAVADNVTA AMEPIAGYSR GHNLAGGRPS ASKTGTVQLG DTSANRDAWM VGYTPSLSTA VWVGTVKGDE PLVTASGAPI YGSGLPSDIW KATMDGALKG TEVESFPKPT EIGGYAGVPA PPPPPPEAPP PAETVIQPTV EIVPGITIPV GPPTTITLAP PPPPGAPPAD NPPP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttcatcg tcgatgtgcc aaggccgggc gacatccgca ccaatcaggt gtccacgatc ctggctagcg acggctccga gatcgccaag atcgttcccc ccgaaggcaa tcgggtcgac gtcaacctca gtcaggtgcc cgagcatgtc cgcgccgcgg tcatcgccgc cgaagaccgc ggcttctact ccaacccggg gttctccttc agcggcttcg cgcgagcgat aaagaacaac ttgttcggcg gtgacctgca aggcggctcc acgatcaccc agcaatacgt caagaacgcg ttggtcggct cggcgcaaca cgggtggagc ggcctgatgc gcaaggccaa ggaactcgtc atcgccacca agatgtcggg ggagtggtcc aaagacgatg tcctgcaggc ctatctcaac atcatctact tcggccgcgg cgcatacggg atttcggcag cgtccaaggc ctacttcgac aagccggtcg agcagctcac cgtgtcggag ggagccctgc tggcggcgtt gattcggcgg ccctccacgc tggacccggc ggtggatccc gatggggcgc tggcgcgctg gaactgggtg ctcgacggca tggtggatac caaggcgttg tccgccaaag accgggccga gcaggcattt cccaggaccg tgccgccgga tcaagcccgc gcggccaacc agaccaccgg gcccaacggg ctgatcgaac gccaggtcac caaagagttg ttggagctgt tcaacatcga tgaacagacc ctgaacacgc aggggctgca ggtcacgacc acgatcgatg cccaagcgca gcaggcggcc gaaaaggcgg tagcgaaata cctcgacggg caggatcccg agatgcgggc cgcggtggtc tccatcgacc cgcacaacgg ggccgtgcgc gcctattacg gcggcgacaa cgccaacggg ttcgacttcg cccaggccgg gctgcagacg ggatcctcgt tcaaggtatt cgccctggtg gccgcccttg aacagggaat tggcctgggc tatgacgtcg acagttcgcc actgacggtg gacggcatca agatcaccaa tgtcgaaggc gagagctgcg gtacctgcaa catcgcgcag gcgctcaaga tgtcgctgaa caccccctac taccggctga tgctcaaact gaagggcggc ccggaggccg tcgccgacgc cgcgcaccag gccggcatcg ccaccagctt cccgggcgtg ccccacacgc tgtccgagga cggcaagggc ggaccgccca acaacgggat tgtgctgggc cagtatcaga cccgggtgat cgacatggct tcggcctacg ccacgctggc cgcatccggg atctatcacc ggccacattt cgtgcagaaa gtcgtcgacg ccgacggccg ggttctcttc gacgcctcca ccgaagacaa caccggtgac cagcgcatcc ccaaggcggt agccgacaac gtcaccgcgg caatggaacc cattgccgga tattcgcgtg gtcacaacct ggccggcggc cggccgtcgg cctccaagac cggcacggtg cagttgggcg acaccagcgc caaccgagac gcctggatgg tcggttacac cccgtcactg tcgacggccg tgtgggtggg aacggtcaag ggtgacgagc cgttggtgac cgcctcgggc gcaccgattt acggctcggg cctgccgtcg gacatctgga aagccaccat ggacggtgca ttgaagggca ccgaggtcga gagcttcccc aagcccaccg agatcggcgg ctacgccggc gtgccggcac cgcctccacc tccaccggag gcgccgccgc ccgcggagac cgtcatccag cccacggtcg agatagtgcc cgggatcacg attcccgtcg ggccgccaac caccatcact cttgctcccc ctccgccacc aggggcgcca cccgccgaca atccgccacc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat