SSGCID: Target Page

MymaA.18528.a

Bifunctional penicillin-binding protein 1A/1B PonA1 (Rv0050 ortholog)

CENTER ID:	MymaA.18528.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.18528.a.B2.GE37201	mature protein		23	690

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.18528.a.B2.PS01935	mature protein		23	690

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.101
RefSeq:	YP_001848395.1
UniProt:	B2HIB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRSAYLAAA VVILLPIVTF TMAYFIVDVP RPGDIRTNQV STILASDGSE IAKIVPPEGN RVDVNLSQVP EHVRAAVIAA EDRGFYSNPG FSFSGFARAI KNNLFGGDLQ GGSTITQQYV KNALVGSAQH GWSGLMRKAK ELVIATKMSG EWSKDDVLQA YLNIIYFGRG AYGISAASKA YFDKPVEQLT VSEGALLAAL IRRPSTLDPA VDPDGALARW NWVLDGMVDT KALSAKDRAE QVFPRTVPPD QARAANQTTG PNGLIERQVT KELLELFNID EQTLNTQGLQ VTTTIDAQAQ QAAEKAVAKY LDGQDPEMRA AVVSIDPHNG AVRAYYGGDN ANGFDFAQAG LQTGSSFKVF ALVAALEQGI GLGYDVDSSP LTVDGIKITN VEGESCGTCN IAQALKMSLN TSYYRLMLKL KGGPEAVADA AHQAGIATSF PGVPHTLSED GKGGPPNNGI VLGQYQTRVI DMASAYATLA ASGIYHRPHF VQKVVNADGR VLFDASTEDN TGDQRIPKAV ADNVTAAMEP IAGYSRGHNL AGGRPSASKT GTVQLGDTSA NRDAWMVGYT PSLSTAVWVG TVKGDEPLVT ASGAPIYGSG LPSDIWKATM DGALKGTEVE SFPKPTEIGG YAGVPAPPPP PPEAPPPSET VIQPTVEIAP GITIPVGPPT TITLAPPPPP GAPPADNPPP

NT Sequence

gtgcggcggt cggcctacct ggccgcggcg gtggtcatcc tgctgcccat cgtcaccttc accatggcct acttcatcgt cgatgtgcca aggccgggcg acatccgcac caatcaggtg tccacgatcc tggctagcga cggctccgag atcgccaaga tcgttccccc cgaaggcaat cgggtcgacg tgaacctcag tcaggtgccc gagcatgtcc gcgccgcggt catcgccgcc gaagaccgcg gtttctactc caacccgggg ttctccttca gcggcttcgc gcgagcgata aagaacaact tgttcggcgg tgacctgcaa ggcggctcca cgatcaccca gcaatacgtc aagaacgcgt tggtcggctc ggcgcaacac gggtggagcg gcctgatgcg caaggccaag gaactcgtca tcgccaccaa gatgtcgggg gagtggtcca aagacgatgt cctgcaggcc tatctcaaca tcatctactt cggccgcggc gcatacggga tttcggcagc gtccaaggcc tacttcgaca agccggtcga gcagctcacc gtgtcggagg gagccttgct ggcggcgttg attcggcggc cctccacgct ggacccggcg gtggatcccg atggggcgct ggcgcgctgg aactgggtgc tcgacggcat ggtggatacc aaggcgttgt ccgccaaaga ccgggccgag caggtatttc ccaggaccgt gccgccggat caagcccgcg cggccaacca gaccaccggg cccaacgggc tgatcgaacg ccaggtcacc aaagagttgt tggagctgtt caacatcgat gaacagaccc tgaacacgca ggggctgcaa gtcacgacca cgatcgatgc ccaagcgcag caggcggccg aaaaggcggt agcgaaatac cttgacgggc aggatcccga gatgcgggcc gcggtggtct ccatcgaccc gcacaacggg gccgtgcgcg cctattacgg cggcgacaac gccaacgggt tcgacttcgc ccaggccggg ctgcagacgg gatcctcgtt caaggtattc gccctggtgg ccgcccttga acagggaatt ggcctgggct atgacgtcga cagttcgcca ctgacggtgg acggcatcaa gatcaccaat gtcgaaggcg agagctgcgg tacctgcaac atcgcgcagg cgctcaagat gtcgctgaac acctcctact accggctgat gctcaaactg aagggcggcc cggaggccgt cgccgacgcc gcgcaccagg ccggcattgc caccagcttc ccgggcgtgc cccacacgct gtccgaggac ggcaagggtg gaccgcccaa caacgggatt gtgctgggcc agtatcagac ccgggtgatc gacatggctt cggcctacgc cacgctggcc gcatccggga tctatcaccg gccacacttc gtgcagaagg tcgtcaacgc cgacggccgg gttctcttcg acgcctccac cgaagacaac accggtgacc agcgcatccc caaggcggta gccgacaatg tcaccgcggc aatggaaccc attgccggat attcgcgggg tcacaacctg gccggcggcc ggccgtcggc ctccaagacc ggcacggtgc agttgggcga caccagcgcc aaccgagacg cctggatggt cggttacacc ccgtcactgt cgacggccgt gtgggtggga acggtcaagg gtgacgagcc gttggtgacc gcctcgggcg caccgattta cggctcgggc ctgccgtcgg acatctggaa agccaccatg gacggtgcat tgaagggcac cgaggtcgag agcttcccca agcccaccga gatcggcggc tacgccggcg tgccggcacc gcctccacct ccaccggagg cgccgccgcc ctcggagacc gtcatccagc ccacggtcga aatagcgccc gggatcacga ttcccgtcgg gccgccaacc accatcactc ttgctccccc tccgccacca ggggcgccac ccgccgacaa tccgccaccg tga

Details for MymaA.18528.a.B2.GE37201

HARVESTED ON:	8/13/2013
SEQUENCED ON:	8/15/2013
EXPECTED MW:	71kDa
OBSERVED MW:	71kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	40

Validated AA Sequence

MLKLKGGPEA VAXAAHQAGI ATSXPGVPHT LSEDGKGGPP NNGIVLGQYQ TRVIDMASAY ATLAASGIYH RPHFVQKVVN ADGRVLFDAS TEDNTGDQRI PKAVADNVTA AMEPIAGYSR GHNLAGGRPS ASKTGTVQLG DTSANRDAWM VGYTPSLSTA VWVGTVKGDE PLVTASGAPI YGSGLPSDIW KATMDGALKG TEVESFPKPT EIGGYAGVPA PPPPPPEAPP PSETVIQPTV EIAPGITIPV GPPTTITLAP PPPPGAPPAD NPPP

Validated NT Sequence

atcttactca cttacggtgg cggattgtcg gcgggtggcg cccctggtgg cggaggggga gcaagagtga tggtggttgg cggcccgacg ggaatcgtga tcccgggcgc tatttcgacc gtgggctgga tgacggtctc cgagggcggc ggcgcctccg gtggaggtgg aggcggtgcc ggcacgccgg cgtagccgcc gatctcggtg ggcttgggga agctctcgac ctcggtgccc ttcaatgcac cgtccatggt ggctttccag atgtccgacg gcaggcccga gccgtaaatc ggtgcgcccg aggcggtcac caacggctcg tcacccttga ccgttcccac ccacacggcc gtcgacagtg acggggtgta accgaccatc caggcgtctc ggttggcgct ggtgtcgccc aactgcaccg tgccggtctt ggaggccgac ggccggccgc cggccaggtt gtgaccccgc gaatatccgg caatgggttc cattgccgcg gtgacattgt cggctaccgc cttggggatg cgctggtcac cggtgttgtc ttcggtggag gcgtcgaaga gaacccggcc gtcggcgttg acgaccttct gcacgaagtg tggccggtga tagatcccgg atgcggccag cgtggcgtag gccgaagcca tgtcgatcac ccgggtctga tactggccca gcacaatccc gttgttgggc ggtccaccct tgccgtcctc ggacagcgtg tggggcacgc ccggnaagct ggtggcaatg ccggcctggt gcgcggcgnc ngcgacggcc tccgggccgc ccttcagttt gagcatcagc cggtagt

Expected Protein Sequence

MAHHHHHHAY FIVDVPRPGD IRTNQVSTIL ASDGSEIAKI VPPEGNRVDV NLSQVPEHVR AAVIAAEDRG FYSNPGFSFS GFARAIKNNL FGGDLQGGST ITQQYVKNAL VGSAQHGWSG LMRKAKELVI ATKMSGEWSK DDVLQAYLNI IYFGRGAYGI SAASKAYFDK PVEQLTVSEG ALLAALIRRP STLDPAVDPD GALARWNWVL DGMVDTKALS AKDRAEQVFP RTVPPDQARA ANQTTGPNGL IERQVTKELL ELFNIDEQTL NTQGLQVTTT IDAQAQQAAE KAVAKYLDGQ DPEMRAAVVS IDPHNGAVRA YYGGDNANGF DFAQAGLQTG SSFKVFALVA ALEQGIGLGY DVDSSPLTVD GIKITNVEGE SCGTCNIAQA LKMSLNTSYY RLMLKLKGGP EAVADAAHQA GIATSFPGVP HTLSEDGKGG PPNNGIVLGQ YQTRVIDMAS AYATLAASGI YHRPHFVQKV VNADGRVLFD ASTEDNTGDQ RIPKAVADNV TAAMEPIAGY SRGHNLAGGR PSASKTGTVQ LGDTSANRDA WMVGYTPSLS TAVWVGTVKG DEPLVTASGA PIYGSGLPSD IWKATMDGAL KGTEVESFPK PTEIGGYAGV PAPPPPPPEA PPPSETVIQP TVEIAPGITI PVGPPTTITL APPPPPGAPP ADNPPP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcctact tcatcgtcga tgtgccaagg ccgggcgaca tccgcaccaa tcaggtgtcc acgatcctgg ctagcgacgg ctccgagatc gccaagatcg ttccccccga aggcaatcgg gtcgacgtga acctcagtca ggtgcccgag catgtccgcg ccgcggtcat cgccgccgaa gaccgcggtt tctactccaa cccggggttc tccttcagcg gcttcgcgcg agcgataaag aacaacttgt tcggcggtga cctgcaaggc ggctccacga tcacccagca atacgtcaag aacgcgttgg tcggctcggc gcaacacggg tggagcggcc tgatgcgcaa ggccaaggaa ctcgtcatcg ccaccaagat gtcgggggag tggtccaaag acgatgtcct gcaggcctat ctcaacatca tctacttcgg ccgcggcgca tacgggattt cggcagcgtc caaggcctac ttcgacaagc cggtcgagca gctcaccgtg tcggagggag ccttgctggc ggcgttgatt cggcggccct ccacgctgga cccggcggtg gatcccgatg gggcgctggc gcgctggaac tgggtgctcg acggcatggt ggataccaag gcgttgtccg ccaaagaccg ggccgagcag gtatttccca ggaccgtgcc gccggatcaa gcccgcgcgg ccaaccagac caccgggccc aacgggctga tcgaacgcca ggtcaccaaa gagttgttgg agctgttcaa catcgatgaa cagaccctga acacgcaggg gctgcaagtc acgaccacga tcgatgccca agcgcagcag gcggccgaaa aggcggtagc gaaatacctt gacgggcagg atcccgagat gcgggccgcg gtggtctcca tcgacccgca caacggggcc gtgcgcgcct attacggcgg cgacaacgcc aacgggttcg acttcgccca ggccgggctg cagacgggat cctcgttcaa ggtattcgcc ctggtggccg cccttgaaca gggaattggc ctgggctatg acgtcgacag ttcgccactg acggtggacg gcatcaagat caccaatgtc gaaggcgaga gctgcggtac ctgcaacatc gcgcaggcgc tcaagatgtc gctgaacacc tcctactacc ggctgatgct caaactgaag ggcggcccgg aggccgtcgc cgacgccgcg caccaggccg gcattgccac cagcttcccg ggcgtgcccc acacgctgtc cgaggacggc aagggtggac cgcccaacaa cgggattgtg ctgggccagt atcagacccg ggtgatcgac atggcttcgg cctacgccac gctggccgca tccgggatct atcaccggcc acacttcgtg cagaaggtcg tcaacgccga cggccgggtt ctcttcgacg cctccaccga agacaacacc ggtgaccagc gcatccccaa ggcggtagcc gacaatgtca ccgcggcaat ggaacccatt gccggatatt cgcggggtca caacctggcc ggcggccggc cgtcggcctc caagaccggc acggtgcagt tgggcgacac cagcgccaac cgagacgcct ggatggtcgg ttacaccccg tcactgtcga cggccgtgtg ggtgggaacg gtcaagggtg acgagccgtt ggtgaccgcc tcgggcgcac cgatttacgg ctcgggcctg ccgtcggaca tctggaaagc caccatggac ggtgcattga agggcaccga ggtcgagagc ttccccaagc ccaccgagat cggcggctac gccggcgtgc cggcaccgcc tccacctcca ccggaggcgc cgccgccctc ggagaccgtc atccagccca cggtcgaaat agcgcccggg atcacgattc ccgtcgggcc gccaaccacc atcactcttg ctccccctcc gccaccaggg gcgccacccg ccgacaatcc gccaccgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for MymaA.18528.a.B2.PS01935

PURIFICATION DATe:	11/5/2013
CONCENTRATION:	14.8mg/ml
OBSERVED MW:	75kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	40

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCTACT TCATCGTCGA TGTGCCAAGG CCGGGCGACA TCCGCACCAA TCAGGTGTCC ACGATCCTGG CTAGCGACGG CTCCGAGATC GCCAAGATCG TTCCCCCCGA AGGCAATCGG GTCGACGTGA ACCTCAGTCA GGTGCCCGAG CATGTCCGCG CCGCGGTCAT CGCCGCCGAA GACCGCGGTT TCTACTCCAA CCCGGGGTTC TCCTTCAGCG GCTTCGCGCG AGCGATAAAG AACAACTTGT TCGGCGGTGA CCTGCAAGGC GGCTCCACGA TCACCCAGCA ATACGTCAAG AACGCGTTGG TCGGCTCGGC GCAACACGGG TGGAGCGGCC TGATGCGCAA GGCCAAGGAA CTCGTCATCG CCACCAAGAT GTCGGGGGAG TGGTCCAAAG ACGATGTCCT GCAGGCCTAT CTCAACATCA TCTACTTCGG CCGCGGCGCA TACGGGATTT CGGCAGCGTC CAAGGCCTAC TTCGACAAGC CGGTCGAGCA GCTCACCGTG TCGGAGGGAG CCTTGCTGGC GGCGTTGATT CGGCGGCCCT CCACGCTGGA CCCGGCGGTG GATCCCGATG GGGCGCTGGC GCGCTGGAAC TGGGTGCTCG ACGGCATGGT GGATACCAAG GCGTTGTCCG CCAAAGACCG GGCCGAGCAG GTATTTCCCA GGACCGTGCC GCCGGATCAA GCCCGCGCGG CCAACCAGAC CACCGGGCCC AACGGGCTGA TCGAACGCCA GGTCACCAAA GAGTTGTTGG AGCTGTTCAA CATCGATGAA CAGACCCTGA ACACGCAGGG GCTGCAAGTC ACGACCACGA TCGATGCCCA AGCGCAGCAG GCGGCCGAAA AGGCGGTAGC GAAATACCTT GACGGGCAGG ATCCCGAGAT GCGGGCCGCG GTGGTCTCCA TCGACCCGCA CAACGGGGCC GTGCGCGCCT ATTACGGCGG CGACAACGCC AACGGGTTCG ACTTCGCCCA GGCCGGGCTG CAGACGGGAT CCTCGTTCAA GGTATTCGCC CTGGTGGCCG CCCTTGAACA GGGAATTGGC CTGGGCTATG ACGTCGACAG TTCGCCACTG ACGGTGGACG GCATCAAGAT CACCAATGTC GAAGGCGAGA GCTGCGGTAC CTGCAACATC GCGCAGGCGC TCAAGATGTC GCTGAACACC TCCTACTACC GGCTGATGCT CAAACTGAAG GGCGGCCCGG AGGCCGTCGC CGACGCCGCG CACCAGGCCG GCATTGCCAC CAGCTTCCCG GGCGTGCCCC ACACGCTGTC CGAGGACGGC AAGGGTGGAC CGCCCAACAA CGGGATTGTG CTGGGCCAGT ATCAGACCCG GGTGATCGAC ATGGCTTCGG CCTACGCCAC GCTGGCCGCA TCCGGGATCT ATCACCGGCC ACACTTCGTG CAGAAGGTCG TCAACGCCGA CGGCCGGGTT CTCTTCGACG CCTCCACCGA AGACAACACC GGTGACCAGC GCATCCCCAA GGCGGTAGCC GACAATGTCA CCGCGGCAAT GGAACCCATT GCCGGATATT CGCGGGGTCA CAACCTGGCC GGCGGCCGGC CGTCGGCCTC CAAGACCGGC ACGGTGCAGT TGGGCGACAC CAGCGCCAAC CGAGACGCCT GGATGGTCGG TTACACCCCG TCACTGTCGA CGGCCGTGTG GGTGGGAACG GTCAAGGGTG ACGAGCCGTT GGTGACCGCC TCGGGCGCAC CGATTTACGG CTCGGGCCTG CCGTCGGACA TCTGGAAAGC CACCATGGAC GGTGCATTGA AGGGCACCGA GGTCGAGAGC TTCCCCAAGC CCACCGAGAT CGGCGGCTAC GCCGGCGTGC CGGCACCGCC TCCACCTCCA CCGGAGGCGC CGCCGCCCTC GGAGACCGTC ATCCAGCCCA CGGTCGAAAT AGCGCCCGGG ATCACGATTC CCGTCGGGCC GCCAACCACC ATCACTCTTG CTCCCCCTCC GCCACCAGGG GCGCCACCCG CCGACAATCC GCCACCGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT