MytuD.12746.a

putative beta-lactamase

CENTER ID:	MytuD.12746.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.12746.a.A1.GE27897	Full length( MytuD.12746.a )		1	250

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_132377
PATRIC ID:	fig\|83332.12.peg.2137
RefSeq:	NP_216429.1
TubercuList:	Rv1913
MTB Network Portal:	Rv1913
UniProt:	O07720

Orthologues for Rv1913

Species	% Identity	% Coverage	Clones
paratuberculosis	75	94	0
avium	73	94	1
marinum	70	94	1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHFDWERLTD SVHRCRLPFC DVTVGLVRGR TGILLVDTGT TLGEATAIAA DVKQIAGCQV THVVLTHKHF DHVLGSSVFD QAEVFCAPEV VEYLRSATDR LREDALSYGA DTAEVDRAIA ALKPPQHGIY DAAVDLGDRT VTITHPGSGH TTADLVVVAP ATGHADGPTV VFTGDLVEES ADPDIDADSD LAAWPATLDR VLAIGGPDAS YVPGHGKVVD AQFVRRQRAW LRTRASRQPR ETPATLPCKR

NT Sequence

GTGCACTTCG ACTGGGAGCG CCTGACCGAC AGCGTGCATC GCTGCCGGCT GCCGTTCTGT GACGTCACCG TTGGGCTGGT CCGGGGCCGC ACCGGAATAC TGCTCGTCGA CACCGGGACC ACCCTCGGCG AAGCAACAGC AATCGCGGCC GACGTCAAGC AGATCGCTGG TTGCCAGGTA ACGCATGTTG TGTTGACACA CAAGCATTTC GACCATGTGC TGGGTTCCTC GGTGTTCGAC CAAGCGGAGG TGTTCTGCGC TCCCGAGGTC GTCGAATACC TACGGTCGGC TACCGACCGG CTCCGCGAAG ATGCCCTGAG CTACGGCGCG GACACAGCTG AGGTTGACCG CGCGATCGCG GCCCTGAAAC CACCTCAGCA CGGGATCTAC GATGCAGCCG TCGATCTCGG GGACCGCACC GTCACCATCA CTCACCCCGG CAGCGGCCAC ACCACAGCAG ATCTCGTCGT GGTGGCGCCG GCCACCGGCC ATGCAGACGG CCCAACGGTG GTCTTCACGG GTGATCTTGT CGAGGAGTCA GCCGATCCTG ATATCGACGC CGATTCCGAC CTGGCGGCCT GGCCGGCAAC GCTTGATCGG GTACTTGCGA TCGGCGGCCC TGACGCCAGC TACGTCCCGG GGCACGGGAA GGTCGTCGAT GCGCAGTTTG TCCGTCGCCA GCGCGCCTGG TTGCGAACAC GTGCGAGCCG CCAGCCTCGT GAAACGCCAG CTACTTTGCC GTGCAAGCGG

Details for MytuD.12746.a.A1.GE27897

HARVESTED ON:	11/3/2009
SEQUENCED ON:	1/13/2010
EXPECTED MW:	29kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVHFDWERL TDSVHRCRLP FCDVTVGLVR GRTGILLVDT GTTLGEATAI AADVKQIAGC QVTHVVLTHK HFDHVLGSSV FDQAEVFCAP EVVEYLRSAT DRLREDALSY GADTAEVDRA IAALKPPQHG IYDAAVDLGD RTVTITHPGS GHTTADLVVV APATGHADGP TVVFTGDLVE ESADPDIDAD SDLAAWPATL DRVLAIGGPD ASYVPGHGKV VDAQFVRRQR AWLRTRASRQ PRETPATLPC

Validated NT Sequence

tgcacggcaa agtagctggc gtttcacgag gctggcggct cgcacgtgtt cgcaaccagg cgcgctggcg acggacaaac tgcgcatcga cgaccttccc gtgccccggg acgtagctgg cgtcagggcc gccgatcgca agtacccgat caagcgttgc cggccaggcc gccaggtcgg aatcggcgtc gatatcagga tcggctgact cctcgacaag atcacccgtg aagaccaccg ttgggccgtc tgcatggccg gtggccggcg ccaccacgac gagatctgct gtggtgtggc cgctgccggg gtgagtgatg gtgacggtgc ggtccccgag atcgacggct gcatcgtaga tcccgtgctg aggtggtttc agggccgcga tcgcgcggtc aacctcagct gtgtccgcgc cgtagctcag ggcatcttcg cggagccggt cggtagccga ccgtaggtat tcgacgacct cgggagcgca gaacacctcc gcttggtcga acaccgagga acccagcaca tggtcgaaat gcttgtgtgt caacacaaca tgcgttacct ggcaaccagc gatctgcttg acgtcggccg cgattgctgt tgcttcgccg agggtggtcc cggtgtcgac gagcagtatt ccggtgcggc cccggaccag cccaacggtg acgtcacaga acggcagccg gcagcgatgc acgctgtcgg tcaggcgctc ccagtcgaag tgcaccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggtat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcacttc gactgggagc gcctgaccga cagcgtgcat cgctgccggc tgccgttctg tgacgtcacc gttgggctgg tccggggccg caccggaata ctgctcgtcg acaccgggac caccctcggc gaagcaacag caatcgcggc cgacgtcaag cagatcgctg gttgccaggt aacgcatgtt gtgttgacac acaagcattt cgaccatgtg ctgggttcct cggtgttcga ccaagcggag gtgttctgcg ctcccgaggt cgtcgaatac ctacggtcgg ctaccgaccg gctccgcgaa gatgccctga gctacggcgc ggacacagct gaggttgacc gcgcgatcgc ggccctgaaa ccacctcagc acgggatcta cgatgcagcc gtcgatctcg gggaccgcac cgtcaccatc actcaccccg gcagcggcca caccacagca gatctcgtcg tggtggcgcc ggccaccggc catgcagacg gcccaacggt ggtcttcacg ggtgatcttg tcgaggagtc agccgatcct gatatcgacg ccgattccga cctggcggcc tggccggcaa cgcttgatcg ggtacttgcg atcggcggcc ctgacgccag ctacgtcccg gggcacggga aggtcgtcga tgcgcagttt gtccgtcgcc agcgcgcctg gttgcgaaca cgtgcgagcc gccagcctcg tgaaacgcca gctactttgc cgtgcaagcg gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa