MymaA.12746.a

putative beta-lactamase

CENTER ID:	MymaA.12746.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.12746.a.A1.GE30242	Full length( MymaA.12746.a )		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_132377
PATRIC ID:	fig\|216594.6.peg.3013
RefSeq:	YP_001851114.1
UniProt:	B2HDC2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNYDWEQLPG SVYRCRLPFC DVTVGLVIGR DDALLVDSGS TLREAAAIQA DIRQLADRRV AHIALTHKHF DHVLGSSAFV GAEIYCAPEV AEYLSSASHQ LREDALHHGA DAGEIDTAIA ALRPPRHPIL DADVHLGDRR VRITHLGRGH TSADLVVVAT PPAAQTGPVV VFTGDLVEES ADPAIDADSD VAAWPATLER LLAIGGPDAI YVPGHGSVVD ADFIRGQRDW LKQRAHPGLR

NT Sequence

GTGAACTACG ACTGGGAACA GCTGCCCGGC AGCGTCTATC GCTGTCGGCT GCCGTTCTGC GACGTCACAG TCGGTTTGGT GATCGGCCGC GACGACGCAC TGCTCGTCGA CTCCGGTTCA ACGCTGCGCG AAGCGGCCGC AATTCAGGCC GACATCCGGC AGCTCGCCGA CCGCCGTGTG GCACACATCG CGTTGACGCA CAAGCATTTC GACCACGTGC TGGGCTCCTC GGCTTTTGTC GGCGCCGAAA TATACTGCGC GCCCGAAGTC GCCGAATACC TCTCCTCGGC TAGCCACCAG CTGCGCGAGG ACGCGCTGCA CCACGGCGCC GATGCCGGGG AGATCGATAC CGCAATCGCA GCCTTGCGAC CGCCTCGGCA CCCGATCTTG GACGCCGACG TCCACCTCGG CGACCGCCGC GTGCGGATCA CCCATCTCGG CCGTGGCCAC ACCAGCGCAG ACCTGGTGGT CGTGGCGACC CCGCCGGCTG CGCAGACCGG ACCGGTCGTC GTCTTCACCG GTGACCTTGT CGAGGAGTCC GCCGACCCCG CCATCGACGC CGATTCTGAC GTCGCGGCAT GGCCCGCAAC ACTCGAGCGT CTACTGGCGA TCGGTGGACC CGACGCCATT TACGTCCCCG GTCACGGCAG CGTCGTTGAC GCCGACTTCA TCCGCGGGCA GCGAGATTGG CTGAAGCAGC GGGCGCACCC CGGCCTGCGC

Details for MymaA.12746.a.A1.GE30242

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	28kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMVNYDWE QLPGSVYRCR LPFCDVTVGL VIGRDDALLV DSGSTLREAA AIQADIRQLA DRRVAHIALT HKHFDHVLGS SAFVGAEIYC APEVAEYLSS ASHQLREDAL HHGADAGEID TAIAALRPPR HPILDADVHL GDRRVRITHL GRGHTSADLV VVATPPAAQT GPVVVFTGDL VEESADPAID ADSDVAAWPA TLERLLAIGG PDAIYVPGHG SVVDADFIRG QRDWLKQRAH PGLR

Validated NT Sequence

ttattagcgc aggccggggt gcgcccgctg cttcagccaa tctcgctgcc cgcggatgaa gtcggcgtca acgacgctgc cgtgaccggg gacgtaaatg gcgtcgggtc caccgatcgc cagtagacgc tcgagtgttg cgggccatgc cgcgacgtca gaatcggcgt cgatggcggg gtcggcggac tcctcgacaa ggtcaccggt gaagacgacg accggtccgg tctgcgcagc cggcggggtc gccacgacca ccaggtctgc gctggtgtgg ccacggccga gatgggtgat ccgcacgcgg cggtcgccga ggtggacgtc ggcgtccaag atcgggtgcc gaggcggtcg caaggctgcg attgcggtat cgatctcccc ggcatcggcg ccgtggtgca gcgcgtcctc gcgcagctgg tggctagccg aggagaggta ttcggcgact tcgggcgcgc agtatatttc ggcgccgaca aaagccgagg agcccagcac gtggtcgaaa tgcttgtgcg tcaacgcgat gtgtgccaca cggcggtcgg cgagctgccg gatgtcggcc tgaattgcgg ccgcttcgcg cagcgttgaa ccggagtcga cgagcagtgc gtcgtcgcgg ccgatcacca aaccgactgt gacgtcgcag aacggcagcc gacagcgata gacgctgccg ggcagctgtt cccagtcgta gttcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVNYDWEQL PGSVYRCRLP FCDVTVGLVI GRDDALLVDS GSTLREAAAI QADIRQLADR RVAHIALTHK HFDHVLGSSA FVGAEIYCAP EVAEYLSSAS HQLREDALHH GADAGEIDTA IAALRPPRHP ILDADVHLGD RRVRITHLGR GHTSADLVVV ATPPAAQTGP VVVFTGDLVE ESADPAIDAD SDVAAWPATL ERLLAIGGPD AIYVPGHGSV VDADFIRGQR DWLKQRAHPG LR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaactac gactgggaac agctgcccgg cagcgtctat cgctgtcggc tgccgttctg cgacgtcaca gtcggtttgg tgatcggccg cgacgacgca ctgctcgtcg actccggttc aacgctgcgc gaagcggccg caattcaggc cgacatccgg cagctcgccg accgccgtgt ggcacacatc gcgttgacgc acaagcattt cgaccacgtg ctgggctcct cggcttttgt cggcgccgaa atatactgcg cgcccgaagt cgccgaatac ctctcctcgg ctagccacca gctgcgcgag gacgcgctgc accacggcgc cgatgccggg gagatcgata ccgcaatcgc agccttgcga ccgcctcggc acccgatctt ggacgccgac gtccacctcg gcgaccgccg cgtgcggatc acccatctcg gccgtggcca caccagcgca gacctggtgg tcgtggcgac cccgccggct gcgcagaccg gaccggtcgt cgtcttcacc ggtgaccttg tcgaggagtc cgccgacccc gccatcgacg ccgattctga cgtcgcggca tggcccgcaa cactcgagcg tctactggcg atcggtggac ccgacgccat ttacgtcccc ggtcacggca gcgtcgttga cgccgacttc atccgcgggc agcgagattg gctgaagcag cgggcgcacc ccggcctgcg caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa