MyavA.12746.a

Metallo-beta-lactamase

CENTER ID:	MyavA.12746.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.12746.a.A1.GE29170	Full length( MyavA.12746.a )		1	237

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_132377
PATRIC ID:	fig\|243243.7.peg.2716
RefSeq:	YP_881970.1
UniProt:	A0A0H2ZYD2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHLAWERLTG SVHRCRLPFC DVTVGLICGR SGTLLVDTGT TLTEAAAIDA DVHRLARRPV SHIALTHKHF DHVLGSSLFT GAEVYCAPEV VEYLGAATDQ IRDDALRHGA DAAEVDAAIA ALRPGQHGIR DARIDLGDRT VTITHLGAGH TASDLAVIVP AARDGEPTVV FTGDLVEESG DPAIDADSDV TAWPATLDRL LALGGPGARY VPGHGRVVDA AFVRRQRDWL RARAATR

NT Sequence

GTGCATCTCG CGTGGGAGCG GCTGACCGGT AGTGTGCATC GCTGCCGGCT CCCGTTCTGC GACGTCACCG TCGGGCTGAT CTGCGGCCGC AGCGGAACCC TCCTCGTCGA CACCGGAACC ACGCTGACCG AAGCGGCCGC GATCGACGCC GACGTCCACC GGCTCGCCCG ACGGCCGGTG AGCCATATTG CCTTGACGCA CAAGCACTTC GACCACGTGC TGGGTTCGTC GTTGTTCACC GGCGCCGAGG TCTACTGCGC GCCGGAGGTG GTCGAGTATC TCGGGGCGGC GACGGATCAA ATCCGCGACG ACGCGCTGCG TCATGGCGCC GACGCCGCCG AGGTGGATGC CGCCATCGCG GCCCTGCGGC CAGGGCAACA CGGGATCCGC GACGCCCGGA TCGACCTGGG GGACCGCACG GTGACGATCA CCCACCTGGG TGCCGGCCAT ACCGCGTCGG ACCTGGCGGT GATCGTGCCC GCGGCGCGCG ACGGCGAACC CACGGTGGTG TTCACCGGTG ATCTGGTCGA GGAATCCGGC GACCCCGCGA TCGACGCCGA CTCCGATGTG ACGGCCTGGC CGGCCACCCT GGACCGGCTG CTGGCGCTCG GCGGGCCCGG GGCCCGTTAC GTGCCCGGGC ACGGCAGGGT CGTCGACGCC GCGTTCGTCC GCCGTCAGCG CGACTGGTTG CGCGCCCGCG CGGCCACCCG G

Details for MyavA.12746.a.A1.GE29170

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	27kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVHLAWERL TGSVHRCRLP FCDVTVGLIC GRSGTLLVDT GTTLTEAAAI DADVHRLARR PVSHIALTHK HFDHVLGSSL FTGAEVYCAP EVVEYLGAAT DQIRDDALRH GADAAEVDAA IAALRPGQHG IRDARIDLGD RTVTITHLGA GHTASDLAVI VPAARDGEPT VVFTGDLVEE SGDPAIDADS DVTAWPATLD RLLALGGPGA RYVPGHGRVV DAAFVRRQRD WLRARAATR

Validated NT Sequence

ttattaccgg gtggccgcgc gggcgcgcaa ccagtcgcgc tgacggcgga cgaacgcggc gtcgacgacc ctgccgtgcc cgggcacgta acgggccccg ggcccgccga gcgccagcag ccggtccagg gtggccggcc aggccgtcac atcggagtcg gcgtcgatcg cggggtcgcc ggattcctcg accagatcac cggtgaacac caccgtgggt tcgccgtcgc gcgccgcggg cacgatcacc gccaggtccg acgcggtatg gccggcaccc aggtgggtga tcgtcaccgt gcggtccccc aggtcgatcc gggcgtcgcg gatcccgtgt tgccctggcc gcagggccgc gatggcggca tccacctcgg cggcgtcggc gccatgacgc agcgcgtcgt cgcggatttg atccgtcgcc gccccgagat actcgaccac ctccggcgcg cagtagacct cggcgccggt gaacaacgac gaacccagca cgtggtcgaa gtgcttgtgc gtcaaggcaa tatggctcac cggccgtcgg gcgagccggt ggacgtcggc gtcgatcgcg gccgcttcgg tcagcgtggt tccggtgtcg acgaggaggg ttccgctgcg gccgcagatc agcccgacgg tgacgtcgca gaacgggagc cggcagcgat gcacactacc ggtcagccgc tcccacgcga gatgcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcatctc gcgtgggagc ggctgaccgg tagtgtgcat cgctgccggc tcccgttctg cgacgtcacc gtcgggctga tctgcggccg cagcggaacc ctcctcgtcg acaccggaac cacgctgacc gaagcggccg cgatcgacgc cgacgtccac cggctcgccc gacggccggt gagccatatt gccttgacgc acaagcactt cgaccacgtg ctgggttcgt cgttgttcac cggcgccgag gtctactgcg cgccggaggt ggtcgagtat ctcggggcgg cgacggatca aatccgcgac gacgcgctgc gtcatggcgc cgacgccgcc gaggtggatg ccgccatcgc ggccctgcgg ccagggcaac acgggatccg cgacgcccgg atcgacctgg gggaccgcac ggtgacgatc acccacctgg gtgccggcca taccgcgtcg gacctggcgg tgatcgtgcc cgcggcgcgc gacggcgaac ccacggtggt gttcaccggt gatctggtcg aggaatccgg cgaccccgcg atcgacgccg actccgatgt gacggcctgg ccggccaccc tggaccggct gctggcgctc ggcgggcccg gggcccgtta cgtgcccggg cacggcaggg tcgtcgacgc cgcgttcgtc cgccgtcagc gcgactggtt gcgcgcccgc gcggccaccc ggaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa