MytuD.10611.a

Probable zinc-dependent alcohol dehydrogenase AdhE2

CENTER ID: MytuD.10611.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.10611.a.MB1.GE30479 Full length( MytuD.10611.a ) 1 361
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2518
RefSeq: NP_216775.1
TubercuList: Rv2259
MTB Network Portal: Rv2259
UniProt: O53533

Orthologues for Rv2259

Species % Identity % Coverage Clones Proteins Structures
ulcerans 90 100 0 0 0
leprae 89 100 0 0 0
paratuberculosis 89 100 0 0 0
smegmatis 84 100 0 0 0
abscessus 83 96 1 0 0
marinum 32 97 1 0 0
ulcerans 32 97 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQTVRGVIA RQKGEPVELV NIVVPDPGPG EAVVDVTACG VCHTDLTYRE GGINDEYPFL LGHEAAGIIE AVGPGVTAVE PGDFVILNWR AVCGQCRACK RGRPRYCFDT FNAEQKMTLT DGTELTAALG IGAFADKTLV HSGQCTKVDP AADPAVAGLL GCGVMAGLGA AINTGGVTRD DTVAVIGCGG VGDAAIAGAA LVGAKRIIAV DTDDTKLDWA RTFGATHTVN AREVDVVQAI GGLTDGFGAD VVIDAVGRPE TYQQAFYARD LAGTVVLVGV PTPDMRLDMP LVDFFSHGGA LKSSWYGDCL PESDFPTLID LYLQGRLPLQ RFVSERIGLE DVEEAFHKMH GGKVLRSVVM L
NT Sequence
ATGAGTCAGA CGGTGCGCGG TGTGATCGCA CGACAAAAGG GCGAACCCGT TGAGCTGGTG AACATTGTCG TCCCGGATCC CGGACCCGGC GAGGCCGTGG TCGACGTCAC CGCCTGCGGG GTATGCCATA CCGACCTGAC CTACCGCGAG GGCGGCATCA ACGACGAATA CCCTTTTCTG CTCGGACACG AGGCCGCGGG CATCATCGAG GCCGTCGGGC CGGGTGTAAC CGCAGTCGAG CCCGGCGACT TCGTGATCCT GAACTGGCGT GCCGTGTGCG GCCAGTGCCG GGCCTGCAAA CGCGGACGGC CCCGCTACTG CTTCGACACC TTTAACGCCG AACAGAAGAT GACGCTGACC GACGGCACCG AGCTCACTGC GGCGTTGGGC ATCGGGGCCT TTGCCGATAA GACGCTGGTG CACTCTGGCC AGTGCACGAA GGTCGATCCG GCTGCCGATC CCGCGGTGGC CGGCCTGCTG GGTTGCGGGG TCATGGCCGG CCTGGGCGCC GCGATCAACA CCGGCGGGGT AACCCGCGAC GACACCGTCG CGGTGATCGG CTGCGGCGGC GTTGGCGATG CCGCGATCGC CGGTGCCGCG CTGGTCGGCG CCAAACGGAT CATCGCGGTC GACACCGATG ACACGAAGCT TGACTGGGCC CGCACCTTCG GCGCCACCCA CACCGTCAAC GCCCGCGAAG TCGACGTCGT CCAGGCCATC GGCGGCCTCA CGGATGGATT CGGCGCGGAC GTGGTGATCG ACGCCGTCGG CCGACCGGAA ACCTACCAGC AGGCCTTCTA CGCCCGCGAT CTCGCCGGAA CCGTTGTGCT GGTGGGTGTT CCGACGCCCG ACATGCGCCT GGACATGCCG CTGGTCGACT TCTTCTCTCA CGGCGGTGCG CTGAAGTCGT CGTGGTACGG CGATTGCCTG CCCGAAAGCG ACTTCCCCAC GCTGATCGAC CTTTACCTGC AGGGCCGGCT GCCGCTGCAG CGGTTCGTTT CCGAACGCAT CGGGCTCGAA GACGTCGAGG AGGCGTTCCA CAAGATGCAT GGCGGCAAGG TATTGCGTTC GGTGGTGATG TTG
Details for MytuD.10611.a.MB1.GE30479
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 82kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 49
Validated AA Sequence
MSQTVRGVIA RQKGEPVELX XXXVPDPGPG EAVVDVTACG VCHTDLTYRE GGINDEYPFL LGHEAAGIIE AVGPGVTAVE PGDFVILNWR AVCGQCRACK RGRPRYCFDT FNAEQKMTLT DGTELTAALG IGAFADXTLV HSGQCTKVDP AADPAXXXXL GCGVMAGLGA AINTGGVTRD DTVAVIGCGG VGDAAIAGAA LVGAXRIIAV DTDDTKLDWA RTFGATHTVX AREVDVVQAI GGLTDGFGAX VVIDAVGRXE TYQQAFYARD LAGTXVLXGV PXXTCXWTXX XXXSXXXXXX LKSSXXXXXX XKXXXXXDXX XXAGXXRCXG XXXNXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
gnnnctcaga nccagggtcc tggttcgatg agtcagacgg tgcgcggtgt gatcgcacga caaaagggcg aacccgttga gctgnngnnn nnnnncgtcc cggatcccgg acccggcgag gccgtggtcg acgtcaccgc ctgcggggta tgccataccg acctgaccta ccgcgagggc ggcatcaacg acgaataccc ttttctgctc ggacacgagg ccgcgggcat catcgaggcc gtcgggccgg gtgtaaccgc agtcgagccc ggcgacttcg tgatcctgaa ctggcgtgcc gtgtgcggcc agtgccgggc ctgcaaacgc ggacggcccc gctactgctt cgacaccttt aacgccgaac agaagatgac gctgaccgac ggcaccgagc tcactgcggc gttgggcatc ggggcctttg ccgatnagac gctggtgcac tctggccagt gcacgaaggt cgatccggct gccgatcccg cgnngncnnn nnngctgggt tgcggggtca tggccggcct gggcgccgcg atcaacaccg gcggggtaac ccgcgacgac accgtcgcgg tgatcggctg cggcggcgtt ggcgatgccg cgatcgccgg tgccgcgctg gtcggcgccn aacggatcat cgcggtcgac accgatgaca cgaagcttga ctgggcccgc accttcggcg ccacccacac cgtcnacgcc cgcgaagtcg acgtcgtcca ggccatcggc ggcctcacgg atggnttcgg cgcnnnngtg gtgatcgacg ccgtcggccg ancggaaacc taccagcagg ccttctacgc ccgcgatctc gccggaaccg ntgtgctggn gggtgttccg anncnnacat gcncctggac atgnngntnn nnnanttcnt nnnntcangn cngtncgctg aagtcntcgt gnnnnnnnnn nnnctgnnna aagcnnnnnn nnnnnnntga tngnncnttn nctgcnggnn nnntgcgntg cagnggnnnn ntnncnaatc nnnnnnnaan nngncnnnnn nnnntcnnnn nnnnnnannn nnnnnngnnn nnnnnnnnnn nnnngnnnnn nnnnnnntnn nnnannnnnn ncnnncannn nnnnnnnnnn nnnnnnnngn nncnnnnnnn nnnnnnnnnn nnana
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MSQTVRGVIA RQKGEPVELV NIVVPDPGPG EAVVDVTACG VCHTDLTYRE GGINDEYPFL LGHEAAGIIE AVGPGVTAVE PGDFVILNWR AVCGQCRACK RGRPRYCFDT FNAEQKMTLT DGTELTAALG IGAFADKTLV HSGQCTKVDP AADPAVAGLL GCGVMAGLGA AINTGGVTRD DTVAVIGCGG VGDAAIAGAA LVGAKRIIAV DTDDTKLDWA RTFGATHTVN AREVDVVQAI GGLTDGFGAD VVIDAVGRPE TYQQAFYARD LAGTVVLVGV PTPDMRLDMP LVDFFSHGGA LKSSWYGDCL PESDFPTLID LYLQGRLPLQ RFVSERIGLE DVEEAFHKMH GGKVLRSVVM L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgag tcagacggtg cgcggtgtga tcgcacgaca aaagggcgaa cccgttgagc tggtgaacat tgtcgtcccg gatcccggac ccggcgaggc cgtggtcgac gtcaccgcct gcggggtatg ccataccgac ctgacctacc gcgagggcgg catcaacgac gaataccctt ttctgctcgg acacgaggcc gcgggcatca tcgaggccgt cgggccgggt gtaaccgcag tcgagcccgg cgacttcgtg atcctgaact ggcgtgccgt gtgcggccag tgccgggcct gcaaacgcgg acggccccgc tactgcttcg acacctttaa cgccgaacag aagatgacgc tgaccgacgg caccgagctc actgcggcgt tgggcatcgg ggcctttgcc gataagacgc tggtgcactc tggccagtgc acgaaggtcg atccggctgc cgatcccgcg gtggccggcc tgctgggttg cggggtcatg gccggcctgg gcgccgcgat caacaccggc ggggtaaccc gcgacgacac cgtcgcggtg atcggctgcg gcggcgttgg cgatgccgcg atcgccggtg ccgcgctggt cggcgccaaa cggatcatcg cggtcgacac cgatgacacg aagcttgact gggcccgcac cttcggcgcc acccacaccg tcaacgcccg cgaagtcgac gtcgtccagg ccatcggcgg cctcacggat ggattcggcg cggacgtggt gatcgacgcc gtcggccgac cggaaaccta ccagcaggcc ttctacgccc gcgatctcgc cggaaccgtt gtgctggtgg gtgttccgac gcccgacatg cgcctggaca tgccgctggt cgacttcttc tctcacggcg gtgcgctgaa gtcgtcgtgg tacggcgatt gcctgcccga aagcgacttc cccacgctga tcgaccttta cctgcagggc cggctgccgc tgcagcggtt cgtttccgaa cgcatcgggc tcgaagacgt cgaggaggcg ttccacaaga tgcatggcgg caaggtattg cgttcggtgg tgatgttgta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a