MyulA.10611.d

Zinc-dependent alcohol dehydrogenase

CENTER ID: MyulA.10611.d
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.10611.d.A1.GE29967 Full length( MyulA.10611.d ) 1 347
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.5518
RefSeq: YP_908165.1
UniProt: A0PWH5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAATMRAERF YADSKTVVLE DVPIPEPGPG EVLVKVAYCG ICHSDLSLIN GTFPTQAPVV TQGHEASGTI VKLGPEVDGW SEGDRVVVAA GRPCQSCPNC RRGDTVNCLR IQLMAFAYDG AWAEYTVAQA AGLTRVPDNV SLDQAAILAD AVSTPFGAVV RTGKVAVGES VGVWGVGGVG THIVQLARLV GAVPVIALDI NPVVLDRALE LGADYAFDSR DDQLHDKLAE ITGGRMLDVA FDAVGLKATF EQALGSLTAG GRLVGVGTSA ESPTVGPTSM FGLTHKQVLG HLGYQNVDIE TLAKLVSLGR LDLSRSISEI VPLEDLSLGI EKLERQDGNP IRILVQP
NT Sequence
ATGGCGGCCA CGATGCGAGC CGAGCGCTTC TACGCCGATT CCAAAACAGT TGTACTCGAA GATGTTCCGA TACCGGAGCC GGGGCCGGGC GAGGTCCTGG TCAAAGTGGC CTACTGCGGG ATCTGCCACT CCGATCTGAG TCTGATCAAC GGCACCTTCC CCACCCAGGC GCCGGTGGTG ACCCAGGGGC ACGAGGCGTC GGGCACCATA GTCAAACTCG GCCCCGAGGT TGACGGCTGG TCCGAGGGCG ACCGCGTCGT GGTGGCCGCC GGCCGGCCGT GCCAGAGCTG CCCGAACTGC CGACGCGGTG ACACGGTGAA TTGCCTGCGG ATCCAGCTGA TGGCGTTCGC CTACGACGGG GCCTGGGCGG AGTACACCGT GGCACAGGCG GCCGGTCTGA CCCGGGTGCC CGACAATGTT TCGCTCGACC AAGCCGCGAT TCTGGCCGAT GCGGTGTCCA CACCGTTCGG TGCGGTGGTG CGGACCGGCA AAGTGGCCGT CGGGGAATCG GTCGGGGTGT GGGGCGTGGG TGGCGTCGGA ACCCACATCG TGCAACTGGC CCGTTTGGTC GGCGCGGTGC CGGTGATCGC CTTGGACATC AATCCCGTGG TGCTCGACCG GGCGCTGGAA CTGGGCGCCG ACTACGCCTT CGACTCCCGC GACGACCAAC TGCACGACAA GCTCGCCGAG ATCACCGGCG GCCGCATGCT CGACGTGGCG TTCGACGCGG TCGGTCTCAA GGCCACCTTC GAGCAGGCGC TCGGATCGCT GACCGCCGGA GGCCGGCTGG TCGGTGTTGG CACGAGTGCC GAATCACCGA CCGTGGGACC CACCTCGATG TTCGGACTGA CCCACAAGCA GGTACTCGGT CACCTCGGCT ACCAGAACGT CGATATCGAA ACCCTGGCGA AGCTGGTTTC ACTTGGGCGC CTGGATCTTT CGCGCTCCAT CAGCGAGATC GTCCCGCTTG AAGACCTGTC GTTGGGTATC GAGAAGCTGG AACGCCAGGA CGGCAACCCG ATCCGCATCC TGGTCCAGCC C
Details for MyulA.10611.d.A1.GE29967
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MAATMRAERF YADSKTVVLE DVPIPEPGPG EVLVKVAYCG ICHSDLSLIN GTFPTQAPVV TQGHEASGTI VKLGPEVDGW SEGDRVVVAA GRPCQSCPNC RRGDTVNCLR IQLMAFAYDG AWAEYTVAQA AGLTRVPDNV SLDQAAILAD AVSTPFGAVV RTGKVAVGES VGVWGVGGVG THIVQLARLV GAVPVIALDI NPVVLDRALE LGADYAFDSR DDQLHDKLAE ITGGRMLDVA FDAVGLKATF EQALGSLTAG GRLVGVGTSA ESPTVGPTSM FGLTHKQVLG HLGYQNVDIE TLAKLVSLGR LDLSRSISEI VPLEDLSLGI EKLERQDGNP IRILVQ
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatggcggc cacgatgcga gccgagcgct tctacgccga ttccaaaaca gttgtactcg aagatgttcc gataccggag ccggggccgg gcgaggtcct ggtcaaagtg gcctactgcg ggatctgcca ctccgatctg agtctgatca acggcacctt ccccacccag gcgccggtgg tgacccaggg gcacgaggcg tcgggcacca tagtcaaact cggccccgag gttgacggct ggtccgaggg cgaccgcgtc gtggtggccg ccggccggcc gtgccagagc tgcccgaact gccgacgcgg tgacacggtg aattgcctgc ggatccagct gatggcgttc gcctacgacg gggcctgggc ggagtacacc gtggcacagg cggccggtct gacccgggtg cccgacaatg tttcgctcga ccaagccgcg attctggccg atgcggtgtc cacaccgttc ggtgcggtgg tgcggaccgg caaagtggcc gtcggggaat cggtcggggt gtggggcgtg ggtggcgtcg gaacccacat cgtgcaactg gcccgtttgg tcggcgcggt gccggtgatc gccttggaca tcaatcccgt ggtgctcgac cgggcgctgg aactgggcgc cgactacgcc ttcgactccc gcgacgacca actgcacgac aagctcgccg agatcaccgg cggccgcatg ctcgacgtgg cgttcgacgc ggtcggtctc aaggccacct tcgagcaggc gctcggatcg ctgaccgccg gaggccggct ggtcggtgtt ggcacgagtg ccgaatcacc gaccgtggga cccacctcga tgttcggact gacccacaag caggtactcg gtcacctcgg ctaccagaac gtcgatatcg aaaccctggc gaagctggtt tcacttgggc gcctggatct ttcgcgctcc atcagcgaga tcgtcccgct tgaagacctg tcgttgggta tcgagaagct ggaacgccag gacggcaacc cgatccgcat cctggtccag t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAATMRAER FYADSKTVVL EDVPIPEPGP GEVLVKVAYC GICHSDLSLI NGTFPTQAPV VTQGHEASGT IVKLGPEVDG WSEGDRVVVA AGRPCQSCPN CRRGDTVNCL RIQLMAFAYD GAWAEYTVAQ AAGLTRVPDN VSLDQAAILA DAVSTPFGAV VRTGKVAVGE SVGVWGVGGV GTHIVQLARL VGAVPVIALD INPVVLDRAL ELGADYAFDS RDDQLHDKLA EITGGRMLDV AFDAVGLKAT FEQALGSLTA GGRLVGVGTS AESPTVGPTS MFGLTHKQVL GHLGYQNVDI ETLAKLVSLG RLDLSRSISE IVPLEDLSLG IEKLERQDGN PIRILVQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggccacg atgcgagccg agcgcttcta cgccgattcc aaaacagttg tactcgaaga tgttccgata ccggagccgg ggccgggcga ggtcctggtc aaagtggcct actgcgggat ctgccactcc gatctgagtc tgatcaacgg caccttcccc acccaggcgc cggtggtgac ccaggggcac gaggcgtcgg gcaccatagt caaactcggc cccgaggttg acggctggtc cgagggcgac cgcgtcgtgg tggccgccgg ccggccgtgc cagagctgcc cgaactgccg acgcggtgac acggtgaatt gcctgcggat ccagctgatg gcgttcgcct acgacggggc ctgggcggag tacaccgtgg cacaggcggc cggtctgacc cgggtgcccg acaatgtttc gctcgaccaa gccgcgattc tggccgatgc ggtgtccaca ccgttcggtg cggtggtgcg gaccggcaaa gtggccgtcg gggaatcggt cggggtgtgg ggcgtgggtg gcgtcggaac ccacatcgtg caactggccc gtttggtcgg cgcggtgccg gtgatcgcct tggacatcaa tcccgtggtg ctcgaccggg cgctggaact gggcgccgac tacgccttcg actcccgcga cgaccaactg cacgacaagc tcgccgagat caccggcggc cgcatgctcg acgtggcgtt cgacgcggtc ggtctcaagg ccaccttcga gcaggcgctc ggatcgctga ccgccggagg ccggctggtc ggtgttggca cgagtgccga atcaccgacc gtgggaccca cctcgatgtt cggactgacc cacaagcagg tactcggtca cctcggctac cagaacgtcg atatcgaaac cctggcgaag ctggtttcac ttgggcgcct ggatctttcg cgctccatca gcgagatcgt cccgcttgaa gacctgtcgt tgggtatcga gaagctggaa cgccaggacg gcaacccgat ccgcatcctg gtccagaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa