SSGCID: Target Page

MyabA.10611.b

Probable zinc-dependent alcohol dehydrogenase AdhE2

CENTER ID:	MyabA.10611.b
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.10611.b.A1.GE30547	Full length( MyabA.10611.b )		1	346

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.1903
RefSeq:	YP_001702606.1
UniProt:	B1MNQ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MELVDIVIPD PGPGEVVVKV QACGVCHTDL TYREGGINDE FPFLLGHEAA GIVETVGEGV TTVEPGDFVI LNWRAVCGVC RACKRGRPQY CFDTFNAAQK MTLTDGTELT PALGIGAFIE KTLVHAGQCT KVDPTADPAV VGLLGCGVMA GLGAAVNTGN VGRDDTVAVI GCGGVGDAAI AGARLAGARK IIAIDTDDTK LAWAKDFGAT DTINARKVDD VVTAIQELTD EFGADVVIDA VGRPETWKQA FYARDLAGTV VLVGVPTPDM TLEMPLIDFF SRGGSLKSSW YGDCLPERDF PTLVSLYQQG RLPLDRFVSE RIGIESIEAA FEKMHHGEVL RSVVVL

NT Sequence

GTGGAGCTGG TGGACATCGT GATCCCTGAT CCGGGCCCCG GTGAGGTCGT GGTGAAGGTG CAGGCCTGCG GGGTATGCCA CACCGATCTG ACCTACCGCG AGGGCGGGAT CAACGACGAG TTCCCGTTCC TGCTCGGCCA TGAGGCGGCG GGGATTGTGG AGACCGTCGG CGAAGGCGTA ACCACCGTCG AGCCGGGCGA TTTCGTGATC CTGAACTGGC GCGCGGTGTG TGGGGTGTGC CGGGCCTGCA AGCGCGGGCG GCCGCAGTAC TGCTTCGACA CCTTCAACGC CGCTCAGAAG ATGACACTCA CCGACGGCAC CGAGCTGACC CCCGCCCTGG GCATCGGCGC ATTCATCGAG AAGACGCTGG TACATGCCGG GCAATGCACC AAGGTCGATC CGACCGCGGA TCCGGCCGTG GTGGGCCTGC TGGGCTGCGG CGTGATGGCC GGACTCGGCG CGGCGGTGAA CACCGGCAAT GTGGGCCGCG ACGACACCGT CGCGGTCATC GGCTGTGGTG GGGTCGGCGA TGCGGCGATC GCCGGGGCCC GGTTGGCGGG GGCGCGCAAG ATCATCGCCA TCGACACCGA TGACACCAAG CTGGCCTGGG CCAAGGACTT CGGGGCCACC GACACCATCA ATGCCCGCAA GGTCGACGAC GTGGTCACCG CGATCCAGGA ACTGACCGAC GAATTCGGCG CCGACGTGGT GATCGACGCG GTAGGCCGCC CCGAAACCTG GAAACAGGCC TTCTACGCAC GCGATCTGGC CGGCACGGTG GTGTTGGTCG GCGTCCCCAC ACCGGATATG ACGCTCGAGA TGCCCTTGAT CGACTTCTTC TCACGCGGCG GCTCGTTGAA GTCCTCCTGG TATGGCGACT GCCTGCCCGA GCGCGACTTC CCCACCCTGG TATCGCTCTA CCAACAGGGC CGACTGCCCC TGGACCGATT CGTCTCCGAG CGCATCGGCA TCGAAAGCAT CGAAGCCGCG TTCGAAAAGA TGCACCATGG CGAGGTCCTG CGCTCCGTAG TGGTGCTC

Details for MyabA.10611.b.A1.GE30547

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	104

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVELVDIVI PDPGPGEVVV KVQACGVCHT DLTYREGGIN DEFPFLLGHE AAGIVETVGE GVTTVEPGDF VILNWRAVCG VCRACKRGRP QYCFDTFNAA QKMTLTDGTE LTPALGIGAF IEKTLVHAGQ CTKVDPTADP AVVGLLGCGV MAGLGAAVNT GNVGRDDTVA VIGCGGVGDA AIAGARLAGA RKIIAIDTDD TKLAWAKDFG ATDTINARKV DDXVTAIQEL TDEFGAXVVI DAVGRPETWK QAFYARDLAX TVVLVGVPTP DMTLEMPLID FFSRGGSXXS SWYGDXLPXR DFPXPXXSLY XXXXLPXXXX RLXXHXXSXS XXXXXKXXXX XXXXAXXXXX XXXXXXXXXX XXXX

Validated NT Sequence

attttgtnnn ntttaagaag gagananacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg agctggtgga catcgtgatc cctgatccgg gccccggtga ggtcgtggtg aaggtgcagg cctgcggggt atgccacacc gatctgacct accgcgaggg cgggatcaac gacgagttcc cgttcctgct cggccatgag gcggcgggga ttgtggagac cgtcggcgaa ggcgtaacca ccgtcgagcc gggcgatttc gtgatcctga actggcgcgc ggtgtgtggg gtgtgccggg cctgcaagcg cgggcggccg cagtactgct tcgacacctt caacgccgct cagaagatga cactcaccga cggcaccgag ctgacccccg ccctgggcat cggcgcattc atcgagaaga cgctggtaca tgccgggcaa tgcaccaagg tcgatccgac cgcggatccg gccgtggtgg gcctgctggg ctgcggcgtg atggccggac tcggcgcggc ggtgaacacc ggcaatgtgg gccgcgacga caccgtcgcg gtcatcggct gtggtggggt cggcgatgcg gcgatcgccg gggcccggtt ggcgggggcg cgcaagatca tcgccatcga caccgatgac accaagctgg cctgggccaa ggacttcggg gccaccgaca ccatcaatgc ccgcaaggtc gacgacntgg tcaccgcgat ccaggaactg accgacgaat tcggcgccna cgtggtgatc gacgcggtag gccgccccga aacctggaaa caggccttct acgcacgcga tctggccngc acggtggtgt tggtcggcgt ccccacaccg gatatgacgc tcgagatgcc cttgatcgac ttcttctcac gcggcggctc gntgaantcc tcctggtatg gcgactgnct gcccgancgn gacttccccc nncccnggnt atcgctctac nnacnngnnn nactgccccn ngnnnngann cgnctncnnn cgcatcngnc atcnnaaagc nncnaannnn nnnnnaaaan nngcancann nnnnnnncnn nngcncnnnn nnnnannann nnnnannnan nnnnnnnngn cnnnnctnnn nannnncnnn nnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVELVDIVI PDPGPGEVVV KVQACGVCHT DLTYREGGIN DEFPFLLGHE AAGIVETVGE GVTTVEPGDF VILNWRAVCG VCRACKRGRP QYCFDTFNAA QKMTLTDGTE LTPALGIGAF IEKTLVHAGQ CTKVDPTADP AVVGLLGCGV MAGLGAAVNT GNVGRDDTVA VIGCGGVGDA AIAGARLAGA RKIIAIDTDD TKLAWAKDFG ATDTINARKV DDVVTAIQEL TDEFGADVVI DAVGRPETWK QAFYARDLAG TVVLVGVPTP DMTLEMPLID FFSRGGSLKS SWYGDCLPER DFPTLVSLYQ QGRLPLDRFV SERIGIESIE AAFEKMHHGE VLRSVVVL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggagctg gtggacatcg tgatccctga tccgggcccc ggtgaggtcg tggtgaaggt gcaggcctgc ggggtatgcc acaccgatct gacctaccgc gagggcggga tcaacgacga gttcccgttc ctgctcggcc atgaggcggc ggggattgtg gagaccgtcg gcgaaggcgt aaccaccgtc gagccgggcg atttcgtgat cctgaactgg cgcgcggtgt gtggggtgtg ccgggcctgc aagcgcgggc ggccgcagta ctgcttcgac accttcaacg ccgctcagaa gatgacactc accgacggca ccgagctgac ccccgccctg ggcatcggcg cattcatcga gaagacgctg gtacatgccg ggcaatgcac caaggtcgat ccgaccgcgg atccggccgt ggtgggcctg ctgggctgcg gcgtgatggc cggactcggc gcggcggtga acaccggcaa tgtgggccgc gacgacaccg tcgcggtcat cggctgtggt ggggtcggcg atgcggcgat cgccggggcc cggttggcgg gggcgcgcaa gatcatcgcc atcgacaccg atgacaccaa gctggcctgg gccaaggact tcggggccac cgacaccatc aatgcccgca aggtcgacga cgtggtcacc gcgatccagg aactgaccga cgaattcggc gccgacgtgg tgatcgacgc ggtaggccgc cccgaaacct ggaaacaggc cttctacgca cgcgatctgg ccggcacggt ggtgttggtc ggcgtcccca caccggatat gacgctcgag atgcccttga tcgacttctt ctcacgcggc ggctcgttga agtcctcctg gtatggcgac tgcctgcccg agcgcgactt ccccaccctg gtatcgctct accaacaggg ccgactgccc ctggaccgat tcgtctccga gcgcatcggc atcgaaagca tcgaagccgc gttcgaaaag atgcaccatg gcgaggtcct gcgctccgta gtggtgctct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa