MytuD.01464.a

PROBABLE PHOSPHOTRIESTERASE PHP (PARATHION HYDROLASE) (PTE) (ARYLDIALKYLPHOSPHATASE) (PARAOXONASE) (A-ESTERASE) (ARYLTRIPHOSPHATASE) (PARAOXON HYDROLASE)

CENTER ID: MytuD.01464.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01464.a.B1.GE27595 Full length( MytuD.01464.a ) 1 326
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.259
RefSeq: NP_214744.1
TubercuList: Rv0230c
MTB Network Portal: Rv0230c
UniProt: P9WHN9

Orthologues for Rv0230c

Species % Identity % Coverage Clones Proteins Structures
avium 93 94 1 0 0
paratuberculosis 92 100 1 1 0
marinum 89 100 1 0 0
ulcerans 89 100 1 1 0
smegmatis 79 100 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPELNTARGP IDTADLGVTL MHEHVFIMTT EIAQNYPEAW GDEDKRVAGA IARLGELKAR GVDTIVDLTV IGLGRYIPRI ARVAAATELN IVVATGLYTY NDVPFYFHYL GPGAQLDGPE IMTDMFVRDI EHGIADTGIK AGILKCATDE PGLTPGVERV LRAVAQAHKR TGAPISTHTH AGLRRGLDQQ RIFAEEGVDL SRVVIGHCGD STDVGYLEEL IAAGSYLGMD RFGVDVISPF QDRVNIVARM CERGHADKMV LSHDACCYFD ALPEELVPVA MPNWHYLHIH NDVIPALKQH GVTDEQLHTM LVDNPRRIFE RQGGYQ
NT Sequence
GTGCCAGAAC TAAATACCGC TCGCGGACCC ATCGACACCG CTGATCTCGG CGTCACGCTG ATGCACGAGC ACGTCTTCAT CATGACCACC GAGATTGCGC AGAACTACCC GGAAGCCTGG GGCGACGAGG ACAAGCGGGT GGCCGGCGCC ATCGCCCGGC TAGGCGAACT CAAGGCCCGC GGCGTGGACA CCATCGTCGA CCTCACGGTG ATCGGGCTGG GCCGATACAT CCCGCGCATC GCCCGGGTGG CCGCGGCCAC CGAGCTGAAC ATCGTCGTGG CCACCGGCTT GTACACCTAC AACGACGTCC CGTTCTACTT CCACTACCTC GGGCCGGGCG CACAGCTGGA CGGCCCGGAG ATCATGACCG ACATGTTCGT CCGCGACATC GAGCACGGCA TCGCCGACAC CGGCATCAAG GCGGGAATCC TCAAGTGCGC CACCGACGAA CCCGGCCTCA CCCCTGGTGT CGAGCGGGTG TTGCGCGCGG TCGCCCAAGC ACACAAACGC ACCGGGGCGC CGATCTCCAC CCACACCCAC GCCGGGCTGC GGCGCGGCCT TGACCAGCAA CGCATCTTCG CCGAGGAGGG GGTGGACCTG AGCCGGGTGG TTATCGGACA CTGCGGCGAC AGCACCGACG TCGGCTACCT GGAAGAGCTC ATCGCCGCCG GCTCCTACCT CGGGATGGAC CGGTTCGGCG TCGACGTGAT CTCACCGTTT CAGGACCGGG TGAACATCGT GGCCCGAATG TGCGAGCGCG GCCATGCCGA CAAGATGGTG CTATCACACG ACGCCTGCTG CTATTTCGAC GCGCTTCCCG AGGAGCTGGT GCCGGTGGCG ATGCCGAATT GGCATTACCT CCACATCCAC AACGACGTCA TCCCCGCACT GAAGCAGCAC GGCGTCACCG ACGAGCAGCT GCACACCATG CTCGTCGACA ACCCGCGCCG CATCTTCGAG CGGCAGGGCG GCTATCAG
Details for MytuD.01464.a.B1.GE27595
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV PELNTARGPI DTADLGVTLM HEHVFIMTTE IAQNYPEAWG DEDKRVAGAI ARLGELKARG VDTIVDLTVI GLGRYIPRIA RVAAATELNI VVATGLYTYN DVPFYFHYLG PGAQLDGPEI MTDMFVRDIE HGIADTGIKA GILKCATDEP GLTPGVERVL RAVAQAHKRT GAPISTHTHA GLRRGLDQQR IFAEEGVDLS RVVIGHCGDS TDVGYLEELI AAGSYLGMDR FGVDVISPFQ DRVNIVARMC ERGHADKMVL SHDACCYFDA LPEELVPVAM PNWHYLHIHN DVIPALKQHG VTDEQLHTML VDNPRRIFER QGGYQ
Validated NT Sequence
tttaagaagg aganatacca tggctcacca ccaccaccac catatggtgc cagaactaaa taccgctcgc ggacccatcg acaccgctga tctcggcgtc acgctgatgc acgagcacgt cttcatcatg accaccgaga ttgcgcagaa ctacccggaa gcctggggcg acgaggacaa gcgggtggcc ggcgccatcg cccggctagg cgaactcaag gcccgcggcg tggacaccat cgtcgacctc acggtgatcg ggctgggccg atacatcccg cgcatcgccc gggtggccgc ggccaccgag ctgaacatcg tcgtggccac cggcttgtac acctacaacg acgtcccgtt ctacttccac tacctcgggc cgggcgcaca gctggacggc ccggagatca tgaccgacat gttcgtccgc gacatcgagc acggcatcgc cgacaccggc atcaaggcgg gaatcctcaa gtgcgccacc gacgaacccg gcctcacccc tggtgtcgag cgggtgttgc gcgcggtcgc ccaagcacac aaacgcaccg gggcgccgat ctccacccac acccacgccg ggctgcggcg cggccttgac cagcaacgca tcttcgccga ggagggggtg gacctgagcc gggtggttat cggacactgc ggcgacagca ccgacgtcgg ctacctggaa gagctcatcg ccgccggctc ctacctcggg atggaccggt tcggcgtcga cgtgatctca ccgtttcagg accgggtgaa catcgtggcc cgaatgtgcg agcgcggcca tgccgacaag atggtgctat cacacgacgc ctgctgctat ttcgacgcgc ttcccgagga gctggtgccg gtggcgatgc cgaattggca ttacctccac atccacaacg acgtcatccc cgcactgaag cagcacggcg tcaccgacga gcagctgcac accatgctcg tcgacaaccc gcgccgcatc ttcgagcggc agggcggcta tcagtaagtg agta
Expected Protein Sequence
MAHHHHHHMV PELNTARGPI DTADLGVTLM HEHVFIMTTE IAQNYPEAWG DEDKRVAGAI ARLGELKARG VDTIVDLTVI GLGRYIPRIA RVAAATELNI VVATGLYTYN DVPFYFHYLG PGAQLDGPEI MTDMFVRDIE HGIADTGIKA GILKCATDEP GLTPGVERVL RAVAQAHKRT GAPISTHTHA GLRRGLDQQR IFAEEGVDLS RVVIGHCGDS TDVGYLEELI AAGSYLGMDR FGVDVISPFQ DRVNIVARMC ERGHADKMVL SHDACCYFDA LPEELVPVAM PNWHYLHIHN DVIPALKQHG VTDEQLHTML VDNPRRIFER QGGYQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc cagaactaaa taccgctcgc ggacccatcg acaccgctga tctcggcgtc acgctgatgc acgagcacgt cttcatcatg accaccgaga ttgcgcagaa ctacccggaa gcctggggcg acgaggacaa gcgggtggcc ggcgccatcg cccggctagg cgaactcaag gcccgcggcg tggacaccat cgtcgacctc acggtgatcg ggctgggccg atacatcccg cgcatcgccc gggtggccgc ggccaccgag ctgaacatcg tcgtggccac cggcttgtac acctacaacg acgtcccgtt ctacttccac tacctcgggc cgggcgcaca gctggacggc ccggagatca tgaccgacat gttcgtccgc gacatcgagc acggcatcgc cgacaccggc atcaaggcgg gaatcctcaa gtgcgccacc gacgaacccg gcctcacccc tggtgtcgag cgggtgttgc gcgcggtcgc ccaagcacac aaacgcaccg gggcgccgat ctccacccac acccacgccg ggctgcggcg cggccttgac cagcaacgca tcttcgccga ggagggggtg gacctgagcc gggtggttat cggacactgc ggcgacagca ccgacgtcgg ctacctggaa gagctcatcg ccgccggctc ctacctcggg atggaccggt tcggcgtcga cgtgatctca ccgtttcagg accgggtgaa catcgtggcc cgaatgtgcg agcgcggcca tgccgacaag atggtgctat cacacgacgc ctgctgctat ttcgacgcgc ttcccgagga gctggtgccg gtggcgatgc cgaattggca ttacctccac atccacaacg acgtcatccc cgcactgaag cagcacggcg tcaccgacga gcagctgcac accatgctcg tcgacaaccc gcgccgcatc ttcgagcggc agggcggcta tcagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat