MymaA.01464.a

Phosphotriesterase Php

CENTER ID: MymaA.01464.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01464.a.A1.GE30182 Full length( MymaA.01464.a ) 1 326
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.532
RefSeq: YP_001848806.1
UniProt: B2HN12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSALNTARGP IDTADLGVTL MHEHVFIMTT EVAQNYPEAW GDEEKRVADA ITRLNELKSR GVDTIVDLTV IGLGRYIPRI ARIAAATDLN IVVATGLYTY NDVPYRFHYQ GPGGMLDGPE IMTDMFVRDI EQGIADTGVK AGILKCATDE PGITPGVERV LRAVAQTHKR TGVPISTHTH AGLRRGLEQQ RIFEEEGVDL SRVIIGHSGD STDVGYLEEL IAAGSYLGMD RFGIDLILPF EDRVSIVATM CERGHADKMV LSHDANCYFD ALPEELTAVA TPNWHYLHIH NDVIPALKER GVTDEQVHTM LVDNPRRIFE RQGGYQ
NT Sequence
GTGTCAGCGC TAAATACCGC TCGCGGACCC ATCGATACCG CTGATCTGGG CGTCACGCTC ATGCACGAGC ACGTGTTCAT CATGACCACC GAAGTCGCCC AGAACTACCC CGAGGCTTGG GGGGACGAAG AGAAGCGGGT GGCTGATGCG ATCACCCGGC TCAACGAATT GAAGTCGCGC GGTGTGGACA CCATCGTCGA CCTGACCGTG ATCGGCCTGG GCCGCTATAT CCCGCGCATC GCCCGGATCG CCGCCGCCAC CGACCTCAAC ATCGTCGTGG CGACGGGCCT CTACACCTAC AACGACGTCC CATATCGTTT CCACTATCAA GGCCCCGGTG GGATGCTCGA CGGCCCGGAG ATCATGACCG ACATGTTCGT CCGGGACATC GAACAGGGCA TCGCCGACAC CGGCGTCAAG GCCGGCATCC TCAAGTGCGC CACCGACGAG CCCGGCATCA CCCCCGGTGT CGAGCGGGTA TTACGTGCGG TGGCCCAGAC GCACAAGCGC ACCGGGGTGC CGATCTCCAC CCACACCCAC GCCGGGCTGC GACGCGGACT CGAGCAACAG CGCATCTTCG AAGAAGAAGG TGTCGACCTG AGCCGGGTGA TCATCGGGCA CTCCGGTGAC AGCACCGACG TCGGCTACCT CGAGGAGCTC ATCGCCGCTG GCTCTTACCT GGGGATGGAC CGCTTCGGCA TCGACCTGAT CCTGCCGTTC GAGGACCGAG TCAGCATCGT GGCGACGATG TGCGAGCGTG GCCACGCCGA CAAGATGGTG CTCTCCCACG ACGCCAACTG CTATTTCGAC GCGCTGCCCG AGGAGCTGAC AGCGGTGGCC ACGCCGAACT GGCACTACCT ACATATCCAC AACGACGTGA TCCCGGCGCT CAAAGAACGA GGGGTGACCG ACGAACAGGT GCACACCATG CTGGTGGACA ATCCGCGCCG GATCTTTGAA CGACAGGGCG GCTACCAG
Details for MymaA.01464.a.A1.GE30182
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MVSALNTARG PIDTADLGVT LMHEHVFIMT TEVAQNYPEA WGDEEKRVAD AITRLNELKS RGVDTIVDLT VIGLGRYIPR IARIAAATDL NIVVATGLYT YNDVPYRFHY QGPGGMLDGP EIMTDMFVRD IEQGIADTGV KAGILKCATD EPGITPGVER VLRAVAQTHK RTGVPISTHT HAGLRRGLEQ QRIFEEEGVD LSRVIIGHSG DSTDVGYLEE LIAAGSYLGM DRFGIDLILP FEDRVSIVAT MCERGHADKM VLSHDANCYF DALPEELTAV ATPNWHYLHI HNDVIPALKE RGVTDEQVHT MLVDNPRRIF ERQGG
Validated NT Sequence
agccgccctg tcgttcaaag atccggcgcg gattgtccac cagcatggtg tgcacctgtt cgtcggtcac ccctcgttct ttgagcgccg ggatcacgtc gttgtggata tgtaggtagt gccagttcgg cgtggccacc gctgtcagct cctcgggcag cgcgtcgaaa tagcagttgg cgtcgtggga gagcaccatc ttgtcggcgt ggccacgctc gcacatcgtc gccacgatgc tgactcggtc ctcgaacggc aggatcaggt cgatgccgaa gcggtccatc cccaggtaag agccagcggc gatgagctcc tcgaggtagc cgacgtcggt gctgtcaccg gagtgcccga tgatcacccg gctcaggtcg acaccttctt cttcgaagat gcgctgttgc tcgagtccgc gtcgcagccc ggcgtgggtg tgggtggaga tcggcacccc ggtgcgcttg tgcgtctggg ccaccgcacg taatacccgc tcgacaccgg gggtgatgcc gggctcgtcg gtggcgcact tgaggatgcc ggccttgacg ccggtgtcgg cgatgccctg ttcgatgtcc cggacgaaca tgtcggtcat gatctccggg ccgtcgagca tcccaccggg gccttgatag tggaaacgat atgggacgtc gttgtaggtg tagaggcccg tcgccacgac gatgttgagg tcggtggcgg cggcgatccg ggcgatgcgc gggatatagc ggcccaggcc gatcacggtc aggtcgacga tggtgtccac accgcgcgac ttcaattcgt tgagccgggt gatcgcatca gccacccgct tctcttcgtc cccccaagcc tcggggtagt tctgggcgac ttcggtggtc atgatgaaca cgtgctcgtg catgagcgtg acgcccagat cagcggtatc gatgggtccg cgagcggtat ttagcgctga caccatcgaa ccaggaccct gggtctgagc ttc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSALNTAR GPIDTADLGV TLMHEHVFIM TTEVAQNYPE AWGDEEKRVA DAITRLNELK SRGVDTIVDL TVIGLGRYIP RIARIAAATD LNIVVATGLY TYNDVPYRFH YQGPGGMLDG PEIMTDMFVR DIEQGIADTG VKAGILKCAT DEPGITPGVE RVLRAVAQTH KRTGVPISTH THAGLRRGLE QQRIFEEEGV DLSRVIIGHS GDSTDVGYLE ELIAAGSYLG MDRFGIDLIL PFEDRVSIVA TMCERGHADK MVLSHDANCY FDALPEELTA VATPNWHYLH IHNDVIPALK ERGVTDEQVH TMLVDNPRRI FERQGGYQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcagcg ctaaataccg ctcgcggacc catcgatacc gctgatctgg gcgtcacgct catgcacgag cacgtgttca tcatgaccac cgaagtcgcc cagaactacc ccgaggcttg gggggacgaa gagaagcggg tggctgatgc gatcacccgg ctcaacgaat tgaagtcgcg cggtgtggac accatcgtcg acctgaccgt gatcggcctg ggccgctata tcccgcgcat cgcccggatc gccgccgcca ccgacctcaa catcgtcgtg gcgacgggcc tctacaccta caacgacgtc ccatatcgtt tccactatca aggccccggt gggatgctcg acggcccgga gatcatgacc gacatgttcg tccgggacat cgaacagggc atcgccgaca ccggcgtcaa ggccggcatc ctcaagtgcg ccaccgacga gcccggcatc acccccggtg tcgagcgggt attacgtgcg gtggcccaga cgcacaagcg caccggggtg ccgatctcca cccacaccca cgccgggctg cgacgcggac tcgagcaaca gcgcatcttc gaagaagaag gtgtcgacct gagccgggtg atcatcgggc actccggtga cagcaccgac gtcggctacc tcgaggagct catcgccgct ggctcttacc tggggatgga ccgcttcggc atcgacctga tcctgccgtt cgaggaccga gtcagcatcg tggcgacgat gtgcgagcgt ggccacgccg acaagatggt gctctcccac gacgccaact gctatttcga cgcgctgccc gaggagctga cagcggtggc cacgccgaac tggcactacc tacatatcca caacgacgtg atcccggcgc tcaaagaacg aggggtgacc gacgaacagg tgcacaccat gctggtggac aatccgcgcc ggatctttga acgacagggc ggctaccaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a