SSGCID: Target Page

MypaA.01464.a

Putative uncharacterized protein

CENTER ID:	MypaA.01464.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01464.a.A1.GE29606	Full length( MypaA.01464.a )		1	326

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01464.a.A1.PS00765	Full length( MypaA.01464.a )		1	326

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.3901
RefSeq:	NP_962602.1
UniProt:	Q73TQ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSELNTARGP IDTAALGVTL MHEHVFIMTT EIAQNYPEAW GDEEQRVADA IDRLNELKAR GVDTIVDLTV IGLGRYIPRI ARVAAATELN IVVATGLYTY NDVPFCFHYL GPGAELGGPE IMTDMFVRDI EQGIADTGIK AGILKCATDA PGVTPGVERV LRAVAQAHKR TGVPISTHTH AGLRRGLDQQ RIFEEEGVDL SRVVIGHSGD STDVGYLEEL IAAGSYLGMD RFGIDAISPF EDRVNIVAQM CERGHADKMV LSHDANCYFD ALPEELVPQV MPNWHYLHIH NDVIPALKQR GVTDEQLHTM LVDNPRRIFE RQGPYG

NT Sequence

GTGTCAGAAC TCAATACCGC TCGCGGGCCC ATCGACACCG CTGCGCTGGG TGTCACCCTG ATGCACGAAC ACGTGTTCAT CATGACCACC GAGATCGCGC AGAACTACCC CGAAGCGTGG GGCGACGAAG AGCAGCGGGT GGCCGACGCC ATCGACCGGC TCAACGAGCT CAAGGCGCGC GGCGTGGACA CCATCGTCGA CCTCACCGTC ATCGGCCTGG GCCGCTACAT TCCGCGCATC GCCCGGGTCG CGGCGGCGAC CGAGCTGAAT ATCGTTGTGG CAACTGGGCT TTACACCTAC AATGATGTGC CCTTCTGTTT CCACTACCTG GGCCCCGGCG CGGAGCTGGG CGGCCCGGAG ATCATGACCG ACATGTTCGT CCGCGACATC GAGCAGGGCA TCGCCGACAC CGGCATCAAG GCCGGCATCC TCAAGTGCGC CACCGACGCA CCCGGCGTCA CCCCGGGCGT CGAGCGGGTG CTGCGGGCGG TGGCGCAGGC GCACAAGCGC ACCGGGGTGC CGATCTCCAC GCACACCCAC GCCGGGCTGC GCCGCGGCCT CGACCAGCAG CGGATCTTCG AGGAGGAGGG CGTCGACCTG AGCCGGGTGG TGATCGGGCA TTCCGGCGAC AGCACCGACG TCGGCTACCT CGAAGAGCTC ATCGCCGCCG GCTCGTATCT GGGCATGGAC CGGTTCGGCA TCGACGCGAT CTCGCCGTTC GAGGACCGGG TGAACATCGT CGCGCAGATG TGCGAGCGCG GCCACGCCGA CAAGATGGTG CTCTCGCACG ACGCCAACTG TTATTTCGAC GCACTTCCCG AGGAGTTGGT GCCCCAGGTC ATGCCGAACT GGCATTACCT GCACATCCAC AACGACGTGA TCCCCGCCCT CAAGCAGCGC GGCGTCACCG ACGAGCAGCT GCACACCATG CTCGTCGACA ACCCGCGGCG CATCTTCGAG CGCCAGGGCC CCTATGGG

Details for MypaA.01464.a.A1.GE29606

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MVSELNTARG PIDTAALGVT LMHEHVFIMT TEIAQNYPEA WGDEEQRVAD AIDRLNELKA RGVDTIVDLT VIGLGRYIPR IARVAAATEL NIVVATGLYT YNDVPFCFHY LGPGAELGGP EIMTDMFVRD IEQGIADTGI KAGILKCATD APGVTPGVER VLRAVAQAHK RTGVPISTHT HAGLRRGLDQ QRIFEEEGVD LSRVVIGHSG DSTDVGYLEE LIAAGSYLGM DRFGIDAISP FEDRVNIVAQ MCERGHADKM VLSHDANCYF DALPEELVPQ VMPNWHYLHI HNDVIPALKQ RGVTDEQLHT MLVDNPRRIF ERQGP

Validated NT Sequence

aggggccctg gcgctcgaag atgcgccgcg ggttgtcgac gagcatggtg tgcagctgct cgtcggtgac gccgcgctgc ttgagggcgg ggatcacgtc gttgtggatg tgcaggtaat gccagttcgg catgacctgg ggcaccaact cctcgggaag tgcgtcgaaa taacagttgg cgtcgtgcga gagcaccatc ttgtcggcgt ggccgcgctc gcacatctgc gcgacgatgt tcacccggtc ctcgaacggc gagatcgcgt cgatgccgaa ccggtccatg cccagatacg agccggcggc gatgagctct tcgaggtagc cgacgtcggt gctgtcgccg gaatgcccga tcaccacccg gctcaggtcg acgccctcct cctcgaagat ccgctgctgg tcgaggccgc ggcgcagccc ggcgtgggtg tgcgtggaga tcggcacccc ggtgcgcttg tgcgcctgcg ccaccgcccg cagcacccgc tcgacgcccg gggtgacgcc gggtgcgtcg gtggcgcact tgaggatgcc ggccttgatg ccggtgtcgg cgatgccctg ctcgatgtcg cggacgaaca tgtcggtcat gatctccggg ccgcccagct ccgcgccggg gcccaggtag tggaaacaga agggcacatc attgtaggtg taaagcccag ttgccacaac gatattcagc tcggtcgccg ccgcgacccg ggcgatgcgc ggaatgtagc ggcccaggcc gatgacggtg aggtcgacga tggtgtccac gccgcgcgcc ttgagctcgt tgagccggtc gatggcgtcg gccacccgct gctcttcgtc gccccacgct tcggggtagt tctgcgcgat ctcggtggtc atgatgaaca cgtgttcgtg catcagggtg acacccagcg cagcggtgtc gatgggcccg cgagcggtat tgagttctga caccatcgaa ccaggaccct gggtctgagc ttccagggta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSELNTAR GPIDTAALGV TLMHEHVFIM TTEIAQNYPE AWGDEEQRVA DAIDRLNELK ARGVDTIVDL TVIGLGRYIP RIARVAAATE LNIVVATGLY TYNDVPFCFH YLGPGAELGG PEIMTDMFVR DIEQGIADTG IKAGILKCAT DAPGVTPGVE RVLRAVAQAH KRTGVPISTH THAGLRRGLD QQRIFEEEGV DLSRVVIGHS GDSTDVGYLE ELIAAGSYLG MDRFGIDAIS PFEDRVNIVA QMCERGHADK MVLSHDANCY FDALPEELVP QVMPNWHYLH IHNDVIPALK QRGVTDEQLH TMLVDNPRRI FERQGPYG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcagaa ctcaataccg ctcgcgggcc catcgacacc gctgcgctgg gtgtcaccct gatgcacgaa cacgtgttca tcatgaccac cgagatcgcg cagaactacc ccgaagcgtg gggcgacgaa gagcagcggg tggccgacgc catcgaccgg ctcaacgagc tcaaggcgcg cggcgtggac accatcgtcg acctcaccgt catcggcctg ggccgctaca ttccgcgcat cgcccgggtc gcggcggcga ccgagctgaa tatcgttgtg gcaactgggc tttacaccta caatgatgtg cccttctgtt tccactacct gggccccggc gcggagctgg gcggcccgga gatcatgacc gacatgttcg tccgcgacat cgagcagggc atcgccgaca ccggcatcaa ggccggcatc ctcaagtgcg ccaccgacgc acccggcgtc accccgggcg tcgagcgggt gctgcgggcg gtggcgcagg cgcacaagcg caccggggtg ccgatctcca cgcacaccca cgccgggctg cgccgcggcc tcgaccagca gcggatcttc gaggaggagg gcgtcgacct gagccgggtg gtgatcgggc attccggcga cagcaccgac gtcggctacc tcgaagagct catcgccgcc ggctcgtatc tgggcatgga ccggttcggc atcgacgcga tctcgccgtt cgaggaccgg gtgaacatcg tcgcgcagat gtgcgagcgc ggccacgccg acaagatggt gctctcgcac gacgccaact gttatttcga cgcacttccc gaggagttgg tgccccaggt catgccgaac tggcattacc tgcacatcca caacgacgtg atccccgccc tcaagcagcg cggcgtcacc gacgagcagc tgcacaccat gctcgtcgac aacccgcggc gcatcttcga gcgccagggc ccctatggga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MypaA.01464.a.A1.PS00765

PURIFICATION DATe:	9/8/2010
CONCENTRATION:	42.1mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCAGAA CTCAATACCG CTCGCGGGCC CATCGACACC GCTGCGCTGG GTGTCACCCT GATGCACGAA CACGTGTTCA TCATGACCAC CGAGATCGCG CAGAACTACC CCGAAGCGTG GGGCGACGAA GAGCAGCGGG TGGCCGACGC CATCGACCGG CTCAACGAGC TCAAGGCGCG CGGCGTGGAC ACCATCGTCG ACCTCACCGT CATCGGCCTG GGCCGCTACA TTCCGCGCAT CGCCCGGGTC GCGGCGGCGA CCGAGCTGAA TATCGTTGTG GCAACTGGGC TTTACACCTA CAATGATGTG CCCTTCTGTT TCCACTACCT GGGCCCCGGC GCGGAGCTGG GCGGCCCGGA GATCATGACC GACATGTTCG TCCGCGACAT CGAGCAGGGC ATCGCCGACA CCGGCATCAA GGCCGGCATC CTCAAGTGCG CCACCGACGC ACCCGGCGTC ACCCCGGGCG TCGAGCGGGT GCTGCGGGCG GTGGCGCAGG CGCACAAGCG CACCGGGGTG CCGATCTCCA CGCACACCCA CGCCGGGCTG CGCCGCGGCC TCGACCAGCA GCGGATCTTC GAGGAGGAGG GCGTCGACCT GAGCCGGGTG GTGATCGGGC ATTCCGGCGA CAGCACCGAC GTCGGCTACC TCGAAGAGCT CATCGCCGCC GGCTCGTATC TGGGCATGGA CCGGTTCGGC ATCGACGCGA TCTCGCCGTT CGAGGACCGG GTGAACATCG TCGCGCAGAT GTGCGAGCGC GGCCACGCCG ACAAGATGGT GCTCTCGCAC GACGCCAACT GTTATTTCGA CGCACTTCCC GAGGAGTTGG TGCCCCAGGT CATGCCGAAC TGGCATTACC TGCACATCCA CAACGACGTG ATCCCCGCCC TCAAGCAGCG CGGCGTCACC GACGAGCAGC TGCACACCAT GCTCGTCGAC AACCCGCGGC GCATCTTCGA GCGCCAGGGC CCCTATGGGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A