MytuD.01381.a
Enoyl-CoA hydratase
| CENTER ID: | MytuD.01381.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01381.a.A1.GE25966 | Full length( MytuD.01381.a ) | 1 | 243 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01381.a.A1.PS00175b | Full length( MytuD.01381.a ) | 1 | 243 |
Structures
3HE2
DEPOSITED: 5/7/2009
DETERMINATION: XRay
CLONE:
PROTEIN: MytuD.01381.a.A1.PS00175
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_221959 |
| PATRIC ID: | fig|83332.12.peg.1008 |
| RefSeq: | NP_215420.1 |
| TubercuList: | Rv0905 |
| MTB Network Portal: | Rv0905 |
| UniProt: | P9WNP1 |
Orthologues for Rv0905
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIGITQAEAV LTIELQRPER RNALNSQLVE ELTQAIRKAG DGSARAIVLT GQGTAFCAGA DLSGDAFAAD YPDRLIELHK AMDASPMPVV GAINGPAIGA GLQLAMQCDL RVVAPDAFFQ FPTSKYGLAL DNWSIRRLSS LVGHGRARAM LLSAEKLTAE IALHTGMANR IGTLADAQAW AAEIARLAPL AIQHAKRVLN DDGAIEEAWP AHKELFDKAW GSQDVIEAQV ARMEKRPPKF QGA
NT Sequence
ATGATCGGTA TCACCCAGGC AGAAGCCGTG CTGACCATTG AGCTGCAACG CCCGGAGCGC CGCAACGCCT TAAATTCCCA GCTGGTCGAG GAGCTTACGC AGGCCATCCG GAAAGCCGGG GATGGATCGG CTCGGGCGAT CGTGCTGACC GGCCAAGGCA CCGCGTTCTG CGCTGGCGCG GACCTGAGCG GAGACGCATT CGCCGCCGAT TATCCCGACC GGCTCATCGA GCTGCACAAG GCGATGGACG CCTCCCCGAT GCCAGTGGTC GGCGCGATCA ACGGTCCCGC CATCGGCGCC GGCTTGCAGC TTGCCATGCA ATGCGACCTG CGGGTTGTCG CGCCCGATGC CTTCTTCCAG TTTCCGACGT CGAAATACGG TCTGGCCCTG GATAACTGGA GCATCCGCCG GCTGTCGTCG TTGGTTGGGC ACGGACGTGC CCGCGCGATG CTGCTCAGCG CGGAAAAGCT GACCGCCGAG ATCGCACTGC ACACCGGAAT GGCGAATCGC ATTGGCACTT TGGCCGACGC CCAGGCCTGG GCCGCCGAGA TCGCCAGGCT GGCACCACTG GCTATCCAGC ACGCCAAGCG GGTGCTCAAC GACGACGGCG CTATCGAGGA AGCGTGGCCG GCCCATAAGG AACTCTTCGA CAAAGCCTGG GGCAGCCAGG ATGTCATCGA AGCGCAGGTT GCCCGGATGG AAAAGCGGCC GCCGAAGTTC CAAGGGGCT
Details for MytuD.01381.a.A1.GE25966
| HARVESTED ON: | 1/9/2009 |
| SEQUENCED ON: | 7/28/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MGTLEAQTQG PGSMIGITQA EAVLTIELQR PERRNALNSQ LVEELTQAIR KAGDGSARAI VLTGQGTAFC AGADLSGDAF AADYPDRLIE LHKAMDASPM PVVGAINGPA IGAGLQLAMQ CDLRVVAPDA FFQFPTSKYG LALDNWSIRR LSSLVGHGRA RAMLLSAEKL TAEIALHTGM ANRIGTLADA QAWAAEIARL APLAIQHAKR VLNDDGAIEE AWPAHKELFD KAWGSQDVIE AQVARMEKRP PKFQGA
Validated NT Sequence
atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgatcggta tcacccaggc agaagccgtg ctgaccattg agctgcaacg cccggagcgc cgcaacgcct taaattccca gctggtcgag gagcttacgc aggccatccg gaaagccggg gatggatcgg ctcgggcgat cgtgctgacc ggccaaggca ccgcgttctg cgctggcgcg gacctgagcg gagacgcatt cgccgccgat tatcccgacc ggctcatcga gctgcacaag gcgatggacg cctccccgat gccagtggtc ggcgcgatca acggtcccgc catcggcgcc ggcttgcagc ttgccatgca atgcgacctg cgggttgtcg cgcccgatgc cttcttccag tttccgacgt cgaaatacgg tctggccctg gataactgga gcatccgccg gctgtcgtcg ttggttgggc acggacgtgc ccgcgcgatg ctgctcagcg cggaaaagct gaccgccgag atcgcactgc acaccggaat ggcgaatcgc attggcactt tggccgacgc ccaggcctgg gccgccgaga tcgccaggct ggcaccactg gctatccagc acgccaagcg ggtgctcaac gacgacggcg ctatcgagga agcgtggccg gcccataagg aactcttcga caaagcctgg ggcagccagg atgtcatcga agcgcaggtt gcccggatgg aaaagcggcc gccgaagttc caaggggctt aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIGITQAEA VLTIELQRPE RRNALNSQLV EELTQAIRKA GDGSARAIVL TGQGTAFCAG ADLSGDAFAA DYPDRLIELH KAMDASPMPV VGAINGPAIG AGLQLAMQCD LRVVAPDAFF QFPTSKYGLA LDNWSIRRLS SLVGHGRARA MLLSAEKLTA EIALHTGMAN RIGTLADAQA WAAEIARLAP LAIQHAKRVL NDDGAIEEAW PAHKELFDKA WGSQDVIEAQ VARMEKRPPK FQGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcggtatc acccaggcag aagccgtgct gaccattgag ctgcaacgcc cggagcgccg caacgcctta aattcccagc tggtcgagga gcttacgcag gccatccgga aagccgggga tggatcggct cgggcgatcg tgctgaccgg ccaaggcacc gcgttctgcg ctggcgcgga cctgagcgga gacgcattcg ccgccgatta tcccgaccgg ctcatcgagc tgcacaaggc gatggacgcc tccccgatgc cagtggtcgg cgcgatcaac ggtcccgcca tcggcgccgg cttgcagctt gccatgcaat gcgacctgcg ggttgtcgcg cccgatgcct tcttccagtt tccgacgtcg aaatacggtc tggccctgga taactggagc atccgccggc tgtcgtcgtt ggttgggcac ggacgtgccc gcgcgatgct gctcagcgcg gaaaagctga ccgccgagat cgcactgcac accggaatgg cgaatcgcat tggcactttg gccgacgccc aggcctgggc cgccgagatc gccaggctgg caccactggc tatccagcac gccaagcggg tgctcaacga cgacggcgct atcgaggaag cgtggccggc ccataaggaa ctcttcgaca aagcctgggg cagccaggat gtcatcgaag cgcaggttgc ccggatggaa aagcggccgc cgaagttcca aggggctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MytuD.01381.a.A1.PS00175b
| PURIFICATION DATe: | 3/18/2009 |
| CONCENTRATION: | 32.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMIGITQA EAVLTIELQR PERRNALNSQ LVEELTQAIR KAGDGSARAI VLTGQGTAFC AGADLSGDAF AADYPDRLIE LHKAMDASPM PVVGAINGPA IGAGLQLAMQ CDLRVVAPDA FFQFPTSKYG LALDNWSIRR LSSLVGHGRA RAMLLSAEKL TAEIALHTGM ANRIGTLADA QAWAAEIARL APLAIQHAKR VLNDDGAIEE AWPAHKELFD KAWGSQDVIE AQVARMEKRP PKFQGA
Validated NT Sequence
atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgatcggta tcacccaggc agaagccgtg ctgaccattg agctgcaacg cccggagcgc cgcaacgcct taaattccca gctggtcgag gagcttacgc aggccatccg gaaagccggg gatggatcgg ctcgggcgat cgtgctgacc ggccaaggca ccgcgttctg cgctggcgcg gacctgagcg gagacgcatt cgccgccgat tatcccgacc ggctcatcga gctgcacaag gcgatggacg cctccccgat gccagtggtc ggcgcgatca acggtcccgc catcggcgcc ggcttgcagc ttgccatgca atgcgacctg cgggttgtcg cgcccgatgc cttcttccag tttccgacgt cgaaatacgg tctggccctg gataactgga gcatccgccg gctgtcgtcg ttggttgggc acggacgtgc ccgcgcgatg ctgctcagcg cggaaaagct gaccgccgag atcgcactgc acaccggaat ggcgaatcgc attggcactt tggccgacgc ccaggcctgg gccgccgaga tcgccaggct ggcaccactg gctatccagc acgccaagcg ggtgctcaac gacgacggcg ctatcgagga agcgtggccg gcccataagg aactcttcga caaagcctgg ggcagccagg atgtcatcga agcgcaggtt gcccggatgg aaaagcggcc gccgaagttc caaggggctt aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIGITQAEA VLTIELQRPE RRNALNSQLV EELTQAIRKA GDGSARAIVL TGQGTAFCAG ADLSGDAFAA DYPDRLIELH KAMDASPMPV VGAINGPAIG AGLQLAMQCD LRVVAPDAFF QFPTSKYGLA LDNWSIRRLS SLVGHGRARA MLLSAEKLTA EIALHTGMAN RIGTLADAQA WAAEIARLAP LAIQHAKRVL NDDGAIEEAW PAHKELFDKA WGSQDVIEAQ VARMEKRPPK FQGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCGGTATC ACCCAGGCAG AAGCCGTGCT GACCATTGAG CTGCAACGCC CGGAGCGCCG CAACGCCTTA AATTCCCAGC TGGTCGAGGA GCTTACGCAG GCCATCCGGA AAGCCGGGGA TGGATCGGCT CGGGCGATCG TGCTGACCGG CCAAGGCACC GCGTTCTGCG CTGGCGCGGA CCTGAGCGGA GACGCATTCG CCGCCGATTA TCCCGACCGG CTCATCGAGC TGCACAAGGC GATGGACGCC TCCCCGATGC CAGTGGTCGG CGCGATCAAC GGTCCCGCCA TCGGCGCCGG CTTGCAGCTT GCCATGCAAT GCGACCTGCG GGTTGTCGCG CCCGATGCCT TCTTCCAGTT TCCGACGTCG AAATACGGTC TGGCCCTGGA TAACTGGAGC ATCCGCCGGC TGTCGTCGTT GGTTGGGCAC GGACGTGCCC GCGCGATGCT GCTCAGCGCG GAAAAGCTGA CCGCCGAGAT CGCACTGCAC ACCGGAATGG CGAATCGCAT TGGCACTTTG GCCGACGCCC AGGCCTGGGC CGCCGAGATC GCCAGGCTGG CACCACTGGC TATCCAGCAC GCCAAGCGGG TGCTCAACGA CGACGGCGCT ATCGAGGAAG CGTGGCCGGC CCATAAGGAA CTCTTCGACA AAGCCTGGGG CAGCCAGGAT GTCATCGAAG CGCAGGTTGC CCGGATGGAA AAGCGGCCGC CGAAGTTCCA AGGGGCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA