SSGCID: Target Page

MysmA.01381.a

Enoyl-CoA hydratase

CENTER ID:	MysmA.01381.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01381.a.A1.GE28926	Full length( MysmA.01381.a )		1	243

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5495
RefSeq:	YP_889873.1
UniProt:	A0R3Y5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIGVTRDGNV LTLELQRPER RNALNCELVD SLREAVENAA SEDIRAIVLT GQGSVFSAGA DLSGDAFAAE LPDKATALNL AIDKAPVPVI GAINGPAIGA GVILAMICDL RVVVPEAYFQ FPVAKYGLAL DNWSIRRLTS LVGYGRARGM LLAAEKLDAE TALQTGMANR IGTLADAQKW AAEIAGFAPL ALQHAKRVLN DDGAYEEPWP AHKELFDRAW KSPDVIEAQV ARIEKRPPRF TGA

NT Sequence

ATGATTGGTG TCACCCGAGA CGGCAATGTC CTGACCCTGG AGCTCCAACG TCCCGAGCGG CGCAACGCGC TGAACTGCGA ACTCGTCGAC AGCCTGCGCG AGGCGGTCGA GAACGCCGCG TCCGAGGACA TCCGCGCGAT CGTGCTCACC GGCCAGGGGT CGGTGTTCAG CGCGGGCGCC GACCTGTCCG GCGACGCGTT CGCCGCCGAA CTGCCCGACA AGGCGACTGC GCTGAACCTC GCGATCGACA AGGCCCCGGT GCCGGTGATC GGCGCGATCA ACGGGCCCGC GATCGGTGCG GGCGTGATCC TCGCGATGAT CTGTGATCTG CGGGTCGTGG TGCCGGAGGC GTACTTCCAG TTCCCGGTCG CGAAATACGG CCTGGCGCTG GACAACTGGA GCATCCGCCG GTTGACGTCG CTGGTGGGCT ACGGCCGCGC GCGGGGCATG CTGTTGGCCG CGGAGAAGCT CGACGCCGAG ACCGCGCTGC AGACGGGCAT GGCCAACCGC ATCGGGACCC TGGCCGACGC GCAGAAGTGG GCGGCCGAGA TCGCGGGGTT CGCACCGCTG GCGCTGCAGC ACGCCAAGCG TGTCCTCAAC GACGACGGCG CCTACGAAGA GCCGTGGCCC GCGCACAAGG AGCTGTTCGA CCGGGCCTGG AAGTCGCCTG ACGTGATCGA GGCCCAGGTG GCCCGCATCG AGAAGCGTCC GCCCAGGTTC ACCGGGGCC

Details for MysmA.01381.a.A1.GE28926

HARVESTED ON:	3/30/2010
SEQUENCED ON:	4/20/2010
EXPECTED MW:	28kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIGVTRDGN VLTLELQRPE RRNALNCELV DSLREAVENA ASEDIRAIVL TGQGSVFSAG ADLSGDAFAA ELPDKATALN LAIDKAPVPV IGAINGPAIG AGVILAMICD LRVVVPEAYF QFPVAKYGLA LDNWSIRRLT SLVGYGRARG MLLAAEKLDA ETALQTGMAN RIGTLADAQK WAAEIAGFAP LALQHAKRVL NDDGAYEEPW PAHKELFDRA WKSPDVIEAQ VARIEKRPPR FTGA

Validated NT Sequence

ttattaggcc ccggtgaacc tgggcggacg cttctcgatg cgggccacct gggcctcgat cacgtcaggc gacttccagg cccggtcgaa cagctccttg tgcgcgggcc acggctcttc gtaggcgccg tcgtcgttga ggacacgctt ggcgtgctgc agcgccagcg gtgcgaaccc cgcgatctcg gccgcccact tctgcgcgtc ggccagggtc ccgatgcggt tggccatgcc cgtctgcagc gcggtctcgg cgtcgagctt ctccgcggcc aacagcatgc cccgcgcgcg gccgtagccc accagcgacg tcaaccggcg gatgctccag ttgtccagcg ccaggccgta tttcgcgacc gggaactgga agtacgcctc cggcaccacg acccgcagat cacagatcat cgcgaggatc acgcccgcac cgatcgcggg cccgttgatc gcgccgatca ccggcaccgg ggccttgtcg atcgcgaggt tcagcgcagt cgccttgtcg ggcagttcgg cggcgaacgc gtcgccggac aggtcggcgc ccgcgctgaa caccgacccc tggccggtga gcacgatcgc gcggatgtcc tcggacgcgg cgttctcgac cgcctcgcgc aggctgtcga cgagttcgca gttcagcgcg ttgcgccgct cgggacgttg gagctccagg gtcaggacat tgccgtctcg ggtgacacca atcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattggtgtc acccgagacg gcaatgtcct gaccctggag ctccaacgtc ccgagcggcg caacgcgctg aactgcgaac tcgtcgacag cctgcgcgag gcggtcgaga acgccgcgtc cgaggacatc cgcgcgatcg tgctcaccgg ccaggggtcg gtgttcagcg cgggcgccga cctgtccggc gacgcgttcg ccgccgaact gcccgacaag gcgactgcgc tgaacctcgc gatcgacaag gccccggtgc cggtgatcgg cgcgatcaac gggcccgcga tcggtgcggg cgtgatcctc gcgatgatct gtgatctgcg ggtcgtggtg ccggaggcgt acttccagtt cccggtcgcg aaatacggcc tggcgctgga caactggagc atccgccggt tgacgtcgct ggtgggctac ggccgcgcgc ggggcatgct gttggccgcg gagaagctcg acgccgagac cgcgctgcag acgggcatgg ccaaccgcat cgggaccctg gccgacgcgc agaagtgggc ggccgagatc gcggggttcg caccgctggc gctgcagcac gccaagcgtg tcctcaacga cgacggcgcc tacgaagagc cgtggcccgc gcacaaggag ctgttcgacc gggcctggaa gtcgcctgac gtgatcgagg cccaggtggc ccgcatcgag aagcgtccgc ccaggttcac cggggccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa