MyabA.01381.a

Probable enoyl-CoA hydratase/isomerase

CENTER ID: MyabA.01381.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01381.a.A1.GE30509 Full length( MyabA.01381.a ) 1 243
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01381.a.A1.PW30386 Full length( MyabA.01381.a ) 1 243

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.995
RefSeq: YP_001701705.1
UniProt: B1MJ95

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIGVTSDGPV TTIELQRPER RNAVTYELAL AFAEEVKKAA ETARAIVVTG QGTSFCAGAD LSSGAPDPDK FADAWQHSIK SVDAADIPVI AAVNGPAIGA GVMLAMVADL RVVSETARFQ FPVAKYGIAL DNWSIRRLTS LVGYGRARAM LLAAEPLDAQ TALQTGMANR IGELADAQAW AAELAGFAPL ALKHAKRVLN DDGAFEDQWP EHKELFDKAW GSQDIIEAQV ARIQKRPPNF QGA
NT Sequence
ATGATCGGTG TAACTAGCGA CGGTCCCGTC ACCACCATTG AGCTGCAGCG CCCGGAGCGG CGTAACGCGG TGACCTATGA GCTCGCGCTC GCCTTCGCCG AGGAGGTCAA GAAGGCCGCG GAAACCGCAC GTGCGATCGT CGTCACGGGG CAGGGCACGT CGTTCTGCGC CGGCGCCGAC CTGTCCAGCG GCGCTCCTGA TCCCGACAAA TTCGCCGATG CGTGGCAGCA CTCGATCAAG AGTGTCGATG CGGCCGACAT CCCGGTCATC GCCGCCGTCA ACGGCCCCGC GATCGGCGCG GGCGTCATGC TGGCAATGGT CGCCGATCTG CGGGTGGTCT CCGAGACCGC GCGTTTCCAG TTCCCCGTCG CCAAATATGG CATCGCCCTT GATAACTGGA GTATCCGTCG GCTGACCTCG CTGGTGGGCT ACGGGCGCGC GCGTGCCATG CTGCTGGCCG CCGAGCCGCT TGACGCGCAG ACGGCATTGC AGACCGGCAT GGCCAACCGC ATCGGCGAAC TCGCCGATGC GCAGGCCTGG GCGGCCGAGC TCGCCGGGTT CGCACCACTT GCTCTCAAGC ACGCCAAGCG CGTGCTCAAT GACGACGGCG CCTTCGAGGA CCAGTGGCCC GAGCACAAGG AACTGTTCGA CAAGGCGTGG GGCAGCCAGG ACATCATCGA GGCCCAGGTC GCCCGAATTC AGAAGCGTCC CCCCAACTTC CAGGGAGCC
Details for MyabA.01381.a.A1.GE30509
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIGVTSDGP VTTIELQRPE RRNAVTYELA LAFAEEVKKA AETARAIVVT GQGTSFCAGA DLSSGAPDPD KFADAWQHSI KSVDAADIPV IAAVNGPAIG AGVMLAMVAD LRVVSETARF QFPVAKYGIA LDNWSIRRLT SLVGYGRARA MLLAAEPLDA QTALQTGMAN RIGELADAQA WAAELAGFAP LALKHAKRVL NDDGAFEDQW PEHKELFDKA WGSQDIIEAQ VARIQKRPPN FQGA
Validated NT Sequence
ttattaggct ccctggaagt tggggggacg cttctgaatt cgggcgacct gggcctcgat gatgtcctgg ctgccccacg ccttgtcgaa cagttccttg tgctcgggcc actggtcctc gaaggcgccg tcgtcattga gcacgcgctt ggcgtgcttg agagcaagtg gtgcgaaccc ggcgagctcg gccgcccagg cctgcgcatc ggcgagttcg ccgatgcggt tggccatgcc ggtctgcaat gccgtctgcg cgtcaagcgg ctcggcggcc agcagcatgg cacgcgcgcg cccgtagccc accagcgagg tcagccgacg gatactccag ttatcaaggg cgatgccata tttggcgacg gggaactgga aacgcgcggt ctcggagacc acccgcagat cggcgaccat tgccagcatg acgcccgcgc cgatcgcggg gccgttgacg gcggcgatga ccgggatgtc ggccgcatcg acactcttga tcgagtgctg ccacgcatcg gcgaatttgt cgggatcagg agcgccgctg gacaggtcgg cgccggcgca gaacgacgtg ccctgccccg tgacgacgat cgcacgtgcg gtttccgcgg ccttcttgac ctcctcggcg aaggcgagcg cgagctcata ggtcaccgcg ttacgccgct ccgggcgctg cagctcaatg gtggtgacgg gaccgtcgct agttacaccg atcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIGVTSDGP VTTIELQRPE RRNAVTYELA LAFAEEVKKA AETARAIVVT GQGTSFCAGA DLSSGAPDPD KFADAWQHSI KSVDAADIPV IAAVNGPAIG AGVMLAMVAD LRVVSETARF QFPVAKYGIA LDNWSIRRLT SLVGYGRARA MLLAAEPLDA QTALQTGMAN RIGELADAQA WAAELAGFAP LALKHAKRVL NDDGAFEDQW PEHKELFDKA WGSQDIIEAQ VARIQKRPPN FQGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcggtgta actagcgacg gtcccgtcac caccattgag ctgcagcgcc cggagcggcg taacgcggtg acctatgagc tcgcgctcgc cttcgccgag gaggtcaaga aggccgcgga aaccgcacgt gcgatcgtcg tcacggggca gggcacgtcg ttctgcgccg gcgccgacct gtccagcggc gctcctgatc ccgacaaatt cgccgatgcg tggcagcact cgatcaagag tgtcgatgcg gccgacatcc cggtcatcgc cgccgtcaac ggccccgcga tcggcgcggg cgtcatgctg gcaatggtcg ccgatctgcg ggtggtctcc gagaccgcgc gtttccagtt ccccgtcgcc aaatatggca tcgcccttga taactggagt atccgtcggc tgacctcgct ggtgggctac gggcgcgcgc gtgccatgct gctggccgcc gagccgcttg acgcgcagac ggcattgcag accggcatgg ccaaccgcat cggcgaactc gccgatgcgc aggcctgggc ggccgagctc gccgggttcg caccacttgc tctcaagcac gccaagcgcg tgctcaatga cgacggcgcc ttcgaggacc agtggcccga gcacaaggaa ctgttcgaca aggcgtgggg cagccaggac atcatcgagg cccaggtcgc ccgaattcag aagcgtcccc ccaacttcca gggagcctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyabA.01381.a.A1.PW30386
PURIFICATION DATe: 1/3/2011
CONCENTRATION: 24.92mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIGVTSDGP VTTIELQRPE RRNAVTYELA LAFAEEVKKA AETARAIVVT GQGTSFCAGA DLSSGAPDPD KFADAWQHSI KSVDAADIPV IAAVNGPAIG AGVMLAMVAD LRVVSETARF QFPVAKYGIA LDNWSIRRLT SLVGYGRARA MLLAAEPLDA QTALQTGMAN RIGELADAQA WAAELAGFAP LALKHAKRVL NDDGAFEDQW PEHKELFDKA WGSQDIIEAQ VARIQKRPPN FQGA
Validated NT Sequence
ttattaggct ccctggaagt tggggggacg cttctgaatt cgggcgacct gggcctcgat gatgtcctgg ctgccccacg ccttgtcgaa cagttccttg tgctcgggcc actggtcctc gaaggcgccg tcgtcattga gcacgcgctt ggcgtgcttg agagcaagtg gtgcgaaccc ggcgagctcg gccgcccagg cctgcgcatc ggcgagttcg ccgatgcggt tggccatgcc ggtctgcaat gccgtctgcg cgtcaagcgg ctcggcggcc agcagcatgg cacgcgcgcg cccgtagccc accagcgagg tcagccgacg gatactccag ttatcaaggg cgatgccata tttggcgacg gggaactgga aacgcgcggt ctcggagacc acccgcagat cggcgaccat tgccagcatg acgcccgcgc cgatcgcggg gccgttgacg gcggcgatga ccgggatgtc ggccgcatcg acactcttga tcgagtgctg ccacgcatcg gcgaatttgt cgggatcagg agcgccgctg gacaggtcgg cgccggcgca gaacgacgtg ccctgccccg tgacgacgat cgcacgtgcg gtttccgcgg ccttcttgac ctcctcggcg aaggcgagcg cgagctcata ggtcaccgcg ttacgccgct ccgggcgctg cagctcaatg gtggtgacgg gaccgtcgct agttacaccg atcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIGVTSDGP VTTIELQRPE RRNAVTYELA LAFAEEVKKA AETARAIVVT GQGTSFCAGA DLSSGAPDPD KFADAWQHSI KSVDAADIPV IAAVNGPAIG AGVMLAMVAD LRVVSETARF QFPVAKYGIA LDNWSIRRLT SLVGYGRARA MLLAAEPLDA QTALQTGMAN RIGELADAQA WAAELAGFAP LALKHAKRVL NDDGAFEDQW PEHKELFDKA WGSQDIIEAQ VARIQKRPPN FQGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCGGTGTA ACTAGCGACG GTCCCGTCAC CACCATTGAG CTGCAGCGCC CGGAGCGGCG TAACGCGGTG ACCTATGAGC TCGCGCTCGC CTTCGCCGAG GAGGTCAAGA AGGCCGCGGA AACCGCACGT GCGATCGTCG TCACGGGGCA GGGCACGTCG TTCTGCGCCG GCGCCGACCT GTCCAGCGGC GCTCCTGATC CCGACAAATT CGCCGATGCG TGGCAGCACT CGATCAAGAG TGTCGATGCG GCCGACATCC CGGTCATCGC CGCCGTCAAC GGCCCCGCGA TCGGCGCGGG CGTCATGCTG GCAATGGTCG CCGATCTGCG GGTGGTCTCC GAGACCGCGC GTTTCCAGTT CCCCGTCGCC AAATATGGCA TCGCCCTTGA TAACTGGAGT ATCCGTCGGC TGACCTCGCT GGTGGGCTAC GGGCGCGCGC GTGCCATGCT GCTGGCCGCC GAGCCGCTTG ACGCGCAGAC GGCATTGCAG ACCGGCATGG CCAACCGCAT CGGCGAACTC GCCGATGCGC AGGCCTGGGC GGCCGAGCTC GCCGGGTTCG CACCACTTGC TCTCAAGCAC GCCAAGCGCG TGCTCAATGA CGACGGCGCC TTCGAGGACC AGTGGCCCGA GCACAAGGAA CTGTTCGACA AGGCGTGGGG CAGCCAGGAC ATCATCGAGG CCCAGGTCGC CCGAATTCAG AAGCGTCCCC CCAACTTCCA GGGAGCCTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA