MytuD.01244.a
6-phosphogluconolactonase
| CENTER ID: | MytuD.01244.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01244.a.A1.GE25953 | Full length( MytuD.01244.a ) | 1 | 247 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.01244.a.A1.PW25959 | Full length( MytuD.01244.a ) | 1 | 247 |
Structures
3ICO
DEPOSITED: 7/17/2009
DETERMINATION: XRay
CLONE: MytuD.01244.a.A1.GE25953
PROTEIN: MytuD.01244.a.A1.PW25959
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127501 |
| PATRIC ID: | fig|83332.12.peg.1614 |
| RefSeq: | NP_215961.1 |
| TubercuList: | Rv1445c |
| MTB Network Portal: | Rv1445c |
| UniProt: | P9WQP5 |
Orthologues for Rv1445c
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSSIEIFPD SDILVAAAGK RLVGAIGAAV AARGQALIVL TGGGNGIALL RYLSAQAQQI EWSKVHLFWG DERYVPEDDD ERNLKQARRA LLNHVDIPSN QVHPMAASDG DFGGDLDAAA LAYEQVLAAS AAPGDPAPNF DVHLLGMGPE GHINSLFPHS PAVLESTRMV VAVDDSPKPP PRRITLTLPA IQRSREVWLL VSGPGKADAV AAAIGGADPV SVPAAGAVGR QNTLWLLDRD AAAKLPS
NT Sequence
GTGAGCAGTA GCATCGAGAT CTTCCCCGAT AGCGACATTC TGGTCGCGGC CGCGGGTAAG CGCTTGGTTG GCGCCATCGG GGCCGCGGTG GCGGCCAGAG GGCAGGCGCT GATCGTGCTG ACCGGGGGCG GCAACGGGAT TGCGCTTCTG CGCTACCTCA GCGCCCAAGC ACAGCAGATT GAATGGTCCA AGGTGCACCT GTTCTGGGGC GACGAACGCT ACGTTCCCGA AGACGATGAC GAGCGCAATC TCAAGCAGGC CCGGCGGGCG TTGCTCAATC ACGTCGACAT TCCATCGAAC CAGGTGCACC CGATGGCCGC CAGTGATGGT GACTTCGGCG GCGATCTGGA CGCCGCGGCC CTGGCCTACG AACAGGTGCT GGCCGCCAGT GCCGCACCAG GTGACCCGGC GCCGAATTTC GACGTCCACC TGCTGGGCAT GGGGCCCGAG GGCCACATTA ACTCGCTGTT CCCGCACAGC CCCGCCGTCC TCGAGAGCAC CCGCATGGTG GTGGCGGTCG ACGACTCGCC GAAACCGCCG CCACGCCGAA TCACCTTGAC CCTGCCGGCG ATTCAGCGTT CCCGCGAGGT GTGGCTGCTG GTTTCCGGGC CGGGTAAGGC CGACGCCGTG GCCGCGGCCA TCGGCGGCGC CGATCCGGTT TCGGTACCGG CGGCCGGGGC CGTCGGGCGT CAGAACACGC TCTGGCTGCT GGACCGCGAC GCCGCCGCCA AGCTTCCGAG T
Details for MytuD.01244.a.A1.GE25953
| HARVESTED ON: | 1/9/2009 |
| SEQUENCED ON: | 7/28/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSSIEIF PDSDILVAAA GKRLVGAIGA AVAARGQALI VLTGGGNGIA LLRYLSAQAQ QIEWSKVHLF WGDERYVPED DDERNLKQAR RALLNHVDIP SNQVHPMAAS DGDFGGDLDA AALAYEQVLA ASAAPGDPAP NFDVHLLGMG PEGHINSLFP HSPAVLESTR MVVAVDDSPK PPPRRITLTL PAIQRSREVW LLVSGPGKAD AVAAAIGGAD PVSVPAAGAV GRQNTLWLLD RDAAAKLPS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcagtagcat cgagatcttc cccgatagcg acattctggt cgcggccgcg ggtaagcgct tggttggcgc catcggggcc gcggtggcgg ccagagggca ggcgctgatc gtgctgaccg ggggcggcaa cgggattgcg cttctgcgct acctcagcgc ccaagcacag cagattgaat ggtccaaggt gcacctgttc tggggcgacg aacgctacgt tcccgaagac gatgacgagc gcaatctcaa gcaggcccgg cgggcgttgc tcaatcacgt cgacattcca tcgaaccagg tgcacccgat ggccgccagt gatggtgact tcggcggcga tctggacgcc gcggccctgg cctacgaaca ggtgctggcc gccagtgccg caccaggtga cccggcgccg aatttcgacg tccacctgct gggcatgggg cccgagggcc acattaactc gctgttcccg cacagccccg ccgtcctcga gagcacccgc atggtggtgg cggtcgacga ctcgccgaaa ccgccgccac gccgaatcac cttgaccctg ccggcgattc agcgttcccg cgaggtgtgg ctgctggttt ccgggccggg taaggccgac gccgtggccg cggccatcgg cggcgccgat ccggtttcgg taccggcggc cggggccgtc gggcgtcaga acacgctctg gctgctggac cgcgacgccg ccgccaagct tccgagttaa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSSIEIF PDSDILVAAA GKRLVGAIGA AVAARGQALI VLTGGGNGIA LLRYLSAQAQ QIEWSKVHLF WGDERYVPED DDERNLKQAR RALLNHVDIP SNQVHPMAAS DGDFGGDLDA AALAYEQVLA ASAAPGDPAP NFDVHLLGMG PEGHINSLFP HSPAVLESTR MVVAVDDSPK PPPRRITLTL PAIQRSREVW LLVSGPGKAD AVAAAIGGAD PVSVPAAGAV GRQNTLWLLD RDAAAKLPS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcagt agcatcgaga tcttccccga tagcgacatt ctggtcgcgg ccgcgggtaa gcgcttggtt ggcgccatcg gggccgcggt ggcggccaga gggcaggcgc tgatcgtgct gaccgggggc ggcaacggga ttgcgcttct gcgctacctc agcgcccaag cacagcagat tgaatggtcc aaggtgcacc tgttctgggg cgacgaacgc tacgttcccg aagacgatga cgagcgcaat ctcaagcagg cccggcgggc gttgctcaat cacgtcgaca ttccatcgaa ccaggtgcac ccgatggccg ccagtgatgg tgacttcggc ggcgatctgg acgccgcggc cctggcctac gaacaggtgc tggccgccag tgccgcacca ggtgacccgg cgccgaattt cgacgtccac ctgctgggca tggggcccga gggccacatt aactcgctgt tcccgcacag ccccgccgtc ctcgagagca cccgcatggt ggtggcggtc gacgactcgc cgaaaccgcc gccacgccga atcaccttga ccctgccggc gattcagcgt tcccgcgagg tgtggctgct ggtttccggg ccgggtaagg ccgacgccgt ggccgcggcc atcggcggcg ccgatccggt ttcggtaccg gcggccgggg ccgtcgggcg tcagaacacg ctctggctgc tggaccgcga cgccgccgcc aagcttccga gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MytuD.01244.a.A1.PW25959
| PURIFICATION DATe: | 3/31/2009 |
| CONCENTRATION: | 26.92mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSSIEIF PDSDILVAAA GKRLVGAIGA AVAARGQALI VLTGGGNGIA LLRYLSAQAQ QIEWSKVHLF WGDERYVPED DDERNLKQAR RALLNHVDIP SNQVHPMAAS DGDFGGDLDA AALAYEQVLA ASAAPGDPAP NFDVHLLGMG PEGHINSLFP HSPAVLESTR MVVAVDDSPK PPPRRITLTL PAIQRSREVW LLVSGPGKAD AVAAAIGGAD PVSVPAAGAV GRQNTLWLLD RDAAAKLPS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcagtagcat cgagatcttc cccgatagcg acattctggt cgcggccgcg ggtaagcgct tggttggcgc catcggggcc gcggtggcgg ccagagggca ggcgctgatc gtgctgaccg ggggcggcaa cgggattgcg cttctgcgct acctcagcgc ccaagcacag cagattgaat ggtccaaggt gcacctgttc tggggcgacg aacgctacgt tcccgaagac gatgacgagc gcaatctcaa gcaggcccgg cgggcgttgc tcaatcacgt cgacattcca tcgaaccagg tgcacccgat ggccgccagt gatggtgact tcggcggcga tctggacgcc gcggccctgg cctacgaaca ggtgctggcc gccagtgccg caccaggtga cccggcgccg aatttcgacg tccacctgct gggcatgggg cccgagggcc acattaactc gctgttcccg cacagccccg ccgtcctcga gagcacccgc atggtggtgg cggtcgacga ctcgccgaaa ccgccgccac gccgaatcac cttgaccctg ccggcgattc agcgttcccg cgaggtgtgg ctgctggttt ccgggccggg taaggccgac gccgtggccg cggccatcgg cggcgccgat ccggtttcgg taccggcggc cggggccgtc gggcgtcaga acacgctctg gctgctggac cgcgacgccg ccgccaagct tccgagttaa taa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSSIEIF PDSDILVAAA GKRLVGAIGA AVAARGQALI VLTGGGNGIA LLRYLSAQAQ QIEWSKVHLF WGDERYVPED DDERNLKQAR RALLNHVDIP SNQVHPMAAS DGDFGGDLDA AALAYEQVLA ASAAPGDPAP NFDVHLLGMG PEGHINSLFP HSPAVLESTR MVVAVDDSPK PPPRRITLTL PAIQRSREVW LLVSGPGKAD AVAAAIGGAD PVSVPAAGAV GRQNTLWLLD RDAAAKLPS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCAGT AGCATCGAGA TCTTCCCCGA TAGCGACATT CTGGTCGCGG CCGCGGGTAA GCGCTTGGTT GGCGCCATCG GGGCCGCGGT GGCGGCCAGA GGGCAGGCGC TGATCGTGCT GACCGGGGGC GGCAACGGGA TTGCGCTTCT GCGCTACCTC AGCGCCCAAG CACAGCAGAT TGAATGGTCC AAGGTGCACC TGTTCTGGGG CGACGAACGC TACGTTCCCG AAGACGATGA CGAGCGCAAT CTCAAGCAGG CCCGGCGGGC GTTGCTCAAT CACGTCGACA TTCCATCGAA CCAGGTGCAC CCGATGGCCG CCAGTGATGG TGACTTCGGC GGCGATCTGG ACGCCGCGGC CCTGGCCTAC GAACAGGTGC TGGCCGCCAG TGCCGCACCA GGTGACCCGG CGCCGAATTT CGACGTCCAC CTGCTGGGCA TGGGGCCCGA GGGCCACATT AACTCGCTGT TCCCGCACAG CCCCGCCGTC CTCGAGAGCA CCCGCATGGT GGTGGCGGTC GACGACTCGC CGAAACCGCC GCCACGCCGA ATCACCTTGA CCCTGCCGGC GATTCAGCGT TCCCGCGAGG TGTGGCTGCT GGTTTCCGGG CCGGGTAAGG CCGACGCCGT GGCCGCGGCC ATCGGCGGCG CCGATCCGGT TTCGGTACCG GCGGCCGGGG CCGTCGGGCG TCAGAACACG CTCTGGCTGC TGGACCGCGA CGCCGCCGCC AAGCTTCCGA GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA