MysmA.01244.a
6-phosphogluconolactonase
| CENTER ID: | MysmA.01244.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01244.a.A1.GE28892 | Full length( MysmA.01244.a ) | 1 | 244 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01244.a.A1.PW28797 | Full length( MysmA.01244.a ) | 1 | 244 |
Structures
3OC6
DEPOSITED: 8/9/2010
DETERMINATION: XRay
CLONE: MysmA.01244.a.A1.GE28892
PROTEIN: MysmA.01244.a.A1.PW28797
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127501 |
| PATRIC ID: | fig|246196.19.peg.3060 |
| RefSeq: | YP_887414.1 |
| UniProt: | A0QWX6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDTVIERHA DTAALVAAAG DRLVDAISSA IGERGQATIV LTGGGTGIGL LKRVRERSGE IDWSKVHIYW GDERFVPQDD DERNDKQARE ALLDHIGIPP VNVHAMAASD GEFGDDLEAA AAGYAQLLSA NFDSSVPGFD VHLLGMGGEG HVNSLFPDTD AVRETERLVV GVSDSPKPPP RRITLTLPAV QNSREVWLVV SGEAKADAVA AAVGGADPVD IPAAGAVGRE RTVWLVDEAA AAKL
NT Sequence
ATGAGCGACA CCGTCATCGA ACGTCACGCG GACACTGCGG CGCTGGTCGC CGCGGCCGGC GACCGGTTGG TGGATGCCAT CTCGTCCGCG ATCGGCGAGC GTGGTCAGGC CACGATCGTC CTGACCGGCG GCGGCACCGG CATCGGCCTG CTCAAGCGGG TCCGTGAGCG CAGCGGCGAG ATCGACTGGT CGAAGGTCCA CATCTACTGG GGCGACGAAC GATTCGTGCC GCAGGACGAC GACGAGCGCA ACGACAAGCA GGCCCGTGAG GCCCTGCTGG ACCACATCGG CATCCCGCCG GTGAACGTGC ACGCGATGGC CGCGAGCGAC GGTGAGTTCG GTGACGATCT CGAGGCCGCC GCGGCCGGAT ATGCGCAGTT GCTCTCCGCC AACTTCGACT CCTCGGTGCC CGGGTTCGAT GTGCATCTGC TCGGCATGGG CGGCGAGGGG CACGTCAACT CGTTGTTCCC CGACACCGAT GCGGTCCGCG AGACCGAGCG CCTCGTCGTC GGCGTCTCCG ACTCCCCCAA GCCGCCGCCG CGGCGAATCA CGTTGACGTT GCCCGCGGTT CAGAACTCGC GCGAGGTGTG GCTCGTGGTG TCGGGTGAGG CCAAGGCCGA CGCCGTGGCC GCGGCGGTCG GCGGTGCCGA TCCCGTGGAC ATCCCGGCCG CGGGCGCCGT GGGCCGTGAG CGCACGGTGT GGCTGGTCGA CGAGGCCGCC GCCGCCAAGT TG
Details for MysmA.01244.a.A1.GE28892
| HARVESTED ON: | 3/23/2010 |
| SEQUENCED ON: | 4/13/2010 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTVIERH ADTAALVAAA GDRLVDAISS AIGERGQATI VLTGGGTGIG LLKRVRERSG EIDWSKVHIY WGDERFVPQD DDERNDKQAR EALLDHIGIP PVNVHAMAAS DGEFGDDLEA AAAGYAQLLS ANFDSSVPGF DVHLLGMGGE GHVNSLFPDT DAVRETERLV VGVSDSPKPP PRRITLTLPA VQNSREVWLV VSGEAKADAV AAAVGGADPV DIPAAGAVGR ERTVWLVDEA AAA
Validated NT Sequence
tggcggcggc ggcctcgtcg accagccaca ccgtgcgctc acggcccacg gcgcccgcgg ccgggatgtc cacgggatcg gcaccgccga ccgccgcggc cacggcgtcg gccttggcct cacccgacac cacgagccac acctcgcgcg agttctgaac cgcgggcaac gtcaacgtga ttcgccgcgg cggcggcttg ggggagtcgg agacgccgac gacgaggcgc tcggtctcgc ggaccgcatc ggtgtcgggg aacaacgagt tgacgtgccc ctcgccgccc atgccgagca gatgcacatc gaacccgggc accgaggagt cgaagttggc ggagagcaac tgcgcatatc cggccgcggc ggcctcgaga tcgtcaccga actcaccgtc gctcgcggcc atcgcgtgca cgttcaccgg cgggatgccg atgtggtcca gcagggcctc acgggcctgc ttgtcgttgc gctcgtcgtc gtcctgcggc acgaatcgtt cgtcgcccca gtagatgtgg accttcgacc agtcgatctc gccgctgcgc tcacggaccc gcttgagcag gccgatgccg gtgccgccgc cggtcaggac gatcgtggcc tgaccacgct cgccgatcgc ggacgagatg gcatccacca accggtcgcc ggccgcggcg accagcgccg cagtgtccgc gtgacgttcg atgacggtgt cgctcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTVIERH ADTAALVAAA GDRLVDAISS AIGERGQATI VLTGGGTGIG LLKRVRERSG EIDWSKVHIY WGDERFVPQD DDERNDKQAR EALLDHIGIP PVNVHAMAAS DGEFGDDLEA AAAGYAQLLS ANFDSSVPGF DVHLLGMGGE GHVNSLFPDT DAVRETERLV VGVSDSPKPP PRRITLTLPA VQNSREVWLV VSGEAKADAV AAAVGGADPV DIPAAGAVGR ERTVWLVDEA AAAKL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgacacc gtcatcgaac gtcacgcgga cactgcggcg ctggtcgccg cggccggcga ccggttggtg gatgccatct cgtccgcgat cggcgagcgt ggtcaggcca cgatcgtcct gaccggcggc ggcaccggca tcggcctgct caagcgggtc cgtgagcgca gcggcgagat cgactggtcg aaggtccaca tctactgggg cgacgaacga ttcgtgccgc aggacgacga cgagcgcaac gacaagcagg cccgtgaggc cctgctggac cacatcggca tcccgccggt gaacgtgcac gcgatggccg cgagcgacgg tgagttcggt gacgatctcg aggccgccgc ggccggatat gcgcagttgc tctccgccaa cttcgactcc tcggtgcccg ggttcgatgt gcatctgctc ggcatgggcg gcgaggggca cgtcaactcg ttgttccccg acaccgatgc ggtccgcgag accgagcgcc tcgtcgtcgg cgtctccgac tcccccaagc cgccgccgcg gcgaatcacg ttgacgttgc ccgcggttca gaactcgcgc gaggtgtggc tcgtggtgtc gggtgaggcc aaggccgacg ccgtggccgc ggcggtcggc ggtgccgatc ccgtggacat cccggccgcg ggcgccgtgg gccgtgagcg cacggtgtgg ctggtcgacg aggccgccgc cgccaagttg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MysmA.01244.a.A1.PW28797
| PURIFICATION DATe: | 6/1/2010 |
| CONCENTRATION: | 28.59mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTVIERH ADTAALVAAA GDRLVDAISS AIGERGQATI VLTGGGTGIG LLKRVRERSG EIDWSKVHIY WGDERFVPQD DDERNDKQAR EALLDHIGIP PVNVHAMAAS DGEFGDDLEA AAAGYAQLLS ANFDSSVPGF DVHLLGMGGE GHVNSLFPDT DAVRETERLV VGVSDSPKPP PRRITLTLPA VQNSREVWLV VSGEAKADAV AAAVGGADPV DIPAAGAVGR ERTVWLVDEA AAA
Validated NT Sequence
tggcggcggc ggcctcgtcg accagccaca ccgtgcgctc acggcccacg gcgcccgcgg ccgggatgtc cacgggatcg gcaccgccga ccgccgcggc cacggcgtcg gccttggcct cacccgacac cacgagccac acctcgcgcg agttctgaac cgcgggcaac gtcaacgtga ttcgccgcgg cggcggcttg ggggagtcgg agacgccgac gacgaggcgc tcggtctcgc ggaccgcatc ggtgtcgggg aacaacgagt tgacgtgccc ctcgccgccc atgccgagca gatgcacatc gaacccgggc accgaggagt cgaagttggc ggagagcaac tgcgcatatc cggccgcggc ggcctcgaga tcgtcaccga actcaccgtc gctcgcggcc atcgcgtgca cgttcaccgg cgggatgccg atgtggtcca gcagggcctc acgggcctgc ttgtcgttgc gctcgtcgtc gtcctgcggc acgaatcgtt cgtcgcccca gtagatgtgg accttcgacc agtcgatctc gccgctgcgc tcacggaccc gcttgagcag gccgatgccg gtgccgccgc cggtcaggac gatcgtggcc tgaccacgct cgccgatcgc ggacgagatg gcatccacca accggtcgcc ggccgcggcg accagcgccg cagtgtccgc gtgacgttcg atgacggtgt cgctcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTVIERH ADTAALVAAA GDRLVDAISS AIGERGQATI VLTGGGTGIG LLKRVRERSG EIDWSKVHIY WGDERFVPQD DDERNDKQAR EALLDHIGIP PVNVHAMAAS DGEFGDDLEA AAAGYAQLLS ANFDSSVPGF DVHLLGMGGE GHVNSLFPDT DAVRETERLV VGVSDSPKPP PRRITLTLPA VQNSREVWLV VSGEAKADAV AAAVGGADPV DIPAAGAVGR ERTVWLVDEA AAAKL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGACACC GTCATCGAAC GTCACGCGGA CACTGCGGCG CTGGTCGCCG CGGCCGGCGA CCGGTTGGTG GATGCCATCT CGTCCGCGAT CGGCGAGCGT GGTCAGGCCA CGATCGTCCT GACCGGCGGC GGCACCGGCA TCGGCCTGCT CAAGCGGGTC CGTGAGCGCA GCGGCGAGAT CGACTGGTCG AAGGTCCACA TCTACTGGGG CGACGAACGA TTCGTGCCGC AGGACGACGA CGAGCGCAAC GACAAGCAGG CCCGTGAGGC CCTGCTGGAC CACATCGGCA TCCCGCCGGT GAACGTGCAC GCGATGGCCG CGAGCGACGG TGAGTTCGGT GACGATCTCG AGGCCGCCGC GGCCGGATAT GCGCAGTTGC TCTCCGCCAA CTTCGACTCC TCGGTGCCCG GGTTCGATGT GCATCTGCTC GGCATGGGCG GCGAGGGGCA CGTCAACTCG TTGTTCCCCG ACACCGATGC GGTCCGCGAG ACCGAGCGCC TCGTCGTCGG CGTCTCCGAC TCCCCCAAGC CGCCGCCGCG GCGAATCACG TTGACGTTGC CCGCGGTTCA GAACTCGCGC GAGGTGTGGC TCGTGGTGTC GGGTGAGGCC AAGGCCGACG CCGTGGCCGC GGCGGTCGGC GGTGCCGATC CCGTGGACAT CCCGGCCGCG GGCGCCGTGG GCCGTGAGCG CACGGTGTGG CTGGTCGACG AGGCCGCCGC CGCCAAGTTG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA