MyabA.01244.a
Probable 6-phosphogluconolactonase
| CENTER ID: | MyabA.01244.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01244.a.A1.GE30487 | Full length( MyabA.01244.a ) | 1 | 247 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01244.a.A1.PS00890 | Full length( MyabA.01244.a ) | 1 | 247 |
Structures
3TX2
DEPOSITED: 9/22/2011
DETERMINATION: XRay
CLONE: MyabA.01244.a.A1.GE30487
PROTEIN: MyabA.01244.a.A1.PS00890
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127501 |
| PATRIC ID: | fig|36809.5.peg.2809 |
| RefSeq: | YP_001703496.1 |
| UniProt: | B1MC71 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSETIIEKYA DTDALVTAAG DRLASAITGA LAERGKAMIV LTGGGTGIAL LKHLRDVASG LDWTNVHVFW GDDRYVPKTD PERNAWQAWE ALLEHVNFPL RNMHAMPNSE SEYGTDLDAA ALAYEQLLAA NAEPGQDCPA FDVHLLGMGG EGHINSLFPH TDAVKETQRL VVAVPDSPKP PPQRITLTLP AIQRSREVWL VVSGEAKADA VAAAVGGADP VDVPAAGAKG IERTVWLLDE AAASQLG
NT Sequence
ATGAGTGAGA CGATCATCGA AAAGTACGCG GACACGGATG CTTTGGTGAC CGCGGCGGGC GATCGGCTGG CATCGGCGAT CACCGGCGCC CTCGCCGAAC GGGGCAAGGC GATGATCGTA CTGACCGGCG GCGGCACCGG CATAGCGCTA CTCAAGCACC TACGCGATGT CGCCAGCGGT CTCGACTGGA CCAACGTCCA CGTGTTTTGG GGCGATGACC GGTACGTCCC CAAGACCGAT CCGGAACGCA ACGCCTGGCA AGCCTGGGAG GCACTGCTGG AGCACGTGAA CTTCCCACTG CGCAACATGC ATGCCATGCC CAACAGCGAA AGCGAGTACG GCACCGATCT TGACGCCGCC GCACTGGCAT ACGAGCAGCT GTTGGCGGCC AATGCCGAGC CCGGCCAGGA TTGCCCGGCA TTTGATGTCC ATCTGCTGGG CATGGGCGGC GAAGGTCATA TCAATTCATT GTTCCCGCAT ACCGATGCCG TCAAGGAGAC GCAGCGGCTC GTCGTGGCGG TGCCCGACTC TCCCAAGCCT CCGCCACAAC GGATCACCTT GACCCTGCCG GCAATTCAGC GCTCACGCGA AGTGTGGCTG GTGGTTTCCG GCGAGGCGAA GGCCGATGCA GTGGCTGCGG CCGTCGGCGG TGCCGACCCT GTCGATGTGC CCGCGGCCGG CGCCAAGGGC ATCGAGCGCA CGGTGTGGCT GCTCGATGAG GCCGCGGCAA GCCAACTGGG C
Details for MyabA.01244.a.A1.GE30487
| HARVESTED ON: | 9/21/2010 |
| SEQUENCED ON: | 9/28/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSETIIEKY ADTDALVTAA GDRLASAITG ALAERGKAMI VLTGGGTGIA LLKHLRDVAS GLDWTNVHVF WGDDRYVPKT DPERNAWQAW EALLEHVNFP LRNMHAMPNS ESEYGTDLDA AALAYEQLLA ANAEPGQDCP AFDVHLLGMG GEGHINSLFP HTDAVKETQR LVVAVPDSPK PPPQRITLTL PAIQRSREVW LVVSGEAKAD AVAAAVGGAD PVDVPAAGAK GIERTVWLLD EAAASQLG
Validated NT Sequence
ttattagccc agttggcttg ccgcggcctc atcgagcagc cacaccgtgc gctcgatgcc cttggcgccg gccgcgggca catcgacagg gtcggcaccg ccgacggccg cagccactgc atcggccttc gcctcgccgg aaaccaccag ccacacttcg cgtgagcgct gaattgccgg cagggtcaag gtgatccgtt gtggcggagg cttgggagag tcgggcaccg ccacgacgag ccgctgcgtc tccttgacgg catcggtatg cgggaacaat gaattgatat gaccttcgcc gcccatgccc agcagatgga catcaaatgc cgggcaatcc tggccgggct cggcattggc cgccaacagc tgctcgtatg ccagtgcggc ggcgtcaaga tcggtgccgt actcgctttc gctgttgggc atggcatgca tgttgcgcag tgggaagttc acgtgctcca gcagtgcctc ccaggcttgc caggcgttgc gttccggatc ggtcttgggg acgtaccggt catcgcccca aaacacgtgg acgttggtcc agtcgagacc gctggcgaca tcgcgtaggt gcttgagtag cgctatgccg gtgccgccgc cggtcagtac gatcatcgcc ttgccccgtt cggcgagggc gccggtgatc gccgatgcca gccgatcgcc cgccgcggtc accaaagcat ccgtgtccgc gtacttttcg atgatcgtct cactcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat ggtat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSETIIEKY ADTDALVTAA GDRLASAITG ALAERGKAMI VLTGGGTGIA LLKHLRDVAS GLDWTNVHVF WGDDRYVPKT DPERNAWQAW EALLEHVNFP LRNMHAMPNS ESEYGTDLDA AALAYEQLLA ANAEPGQDCP AFDVHLLGMG GEGHINSLFP HTDAVKETQR LVVAVPDSPK PPPQRITLTL PAIQRSREVW LVVSGEAKAD AVAAAVGGAD PVDVPAAGAK GIERTVWLLD EAAASQLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgagacg atcatcgaaa agtacgcgga cacggatgct ttggtgaccg cggcgggcga tcggctggca tcggcgatca ccggcgccct cgccgaacgg ggcaaggcga tgatcgtact gaccggcggc ggcaccggca tagcgctact caagcaccta cgcgatgtcg ccagcggtct cgactggacc aacgtccacg tgttttgggg cgatgaccgg tacgtcccca agaccgatcc ggaacgcaac gcctggcaag cctgggaggc actgctggag cacgtgaact tcccactgcg caacatgcat gccatgccca acagcgaaag cgagtacggc accgatcttg acgccgccgc actggcatac gagcagctgt tggcggccaa tgccgagccc ggccaggatt gcccggcatt tgatgtccat ctgctgggca tgggcggcga aggtcatatc aattcattgt tcccgcatac cgatgccgtc aaggagacgc agcggctcgt cgtggcggtg cccgactctc ccaagcctcc gccacaacgg atcaccttga ccctgccggc aattcagcgc tcacgcgaag tgtggctggt ggtttccggc gaggcgaagg ccgatgcagt ggctgcggcc gtcggcggtg ccgaccctgt cgatgtgccc gcggccggcg ccaagggcat cgagcgcacg gtgtggctgc tcgatgaggc cgcggcaagc caactgggct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyabA.01244.a.A1.PS00890
| PURIFICATION DATe: | 12/7/2010 |
| CONCENTRATION: | 51.9mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSETIIEKY ADTDALVTAA GDRLASAITG ALAERGKAMI VLTGGGTGIA LLKHLRDVAS GLDWTNVHVF WGDDRYVPKT DPERNAWQAW EALLEHVNFP LRNMHAMPNS ESEYGTDLDA AALAYEQLLA ANAEPGQDCP AFDVHLLGMG GEGHINSLFP HTDAVKETQR LVVAVPDSPK PPPQRITLTL PAIQRSREVW LVVSGEAKAD AVAAAVGGAD PVDVPAAGAK GIERTVWLLD EAAASQLG
Validated NT Sequence
ttattagccc agttggcttg ccgcggcctc atcgagcagc cacaccgtgc gctcgatgcc cttggcgccg gccgcgggca catcgacagg gtcggcaccg ccgacggccg cagccactgc atcggccttc gcctcgccgg aaaccaccag ccacacttcg cgtgagcgct gaattgccgg cagggtcaag gtgatccgtt gtggcggagg cttgggagag tcgggcaccg ccacgacgag ccgctgcgtc tccttgacgg catcggtatg cgggaacaat gaattgatat gaccttcgcc gcccatgccc agcagatgga catcaaatgc cgggcaatcc tggccgggct cggcattggc cgccaacagc tgctcgtatg ccagtgcggc ggcgtcaaga tcggtgccgt actcgctttc gctgttgggc atggcatgca tgttgcgcag tgggaagttc acgtgctcca gcagtgcctc ccaggcttgc caggcgttgc gttccggatc ggtcttgggg acgtaccggt catcgcccca aaacacgtgg acgttggtcc agtcgagacc gctggcgaca tcgcgtaggt gcttgagtag cgctatgccg gtgccgccgc cggtcagtac gatcatcgcc ttgccccgtt cggcgagggc gccggtgatc gccgatgcca gccgatcgcc cgccgcggtc accaaagcat ccgtgtccgc gtacttttcg atgatcgtct cactcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat ggtat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSETIIEKY ADTDALVTAA GDRLASAITG ALAERGKAMI VLTGGGTGIA LLKHLRDVAS GLDWTNVHVF WGDDRYVPKT DPERNAWQAW EALLEHVNFP LRNMHAMPNS ESEYGTDLDA AALAYEQLLA ANAEPGQDCP AFDVHLLGMG GEGHINSLFP HTDAVKETQR LVVAVPDSPK PPPQRITLTL PAIQRSREVW LVVSGEAKAD AVAAAVGGAD PVDVPAAGAK GIERTVWLLD EAAASQLG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGAGACG ATCATCGAAA AGTACGCGGA CACGGATGCT TTGGTGACCG CGGCGGGCGA TCGGCTGGCA TCGGCGATCA CCGGCGCCCT CGCCGAACGG GGCAAGGCGA TGATCGTACT GACCGGCGGC GGCACCGGCA TAGCGCTACT CAAGCACCTA CGCGATGTCG CCAGCGGTCT CGACTGGACC AACGTCCACG TGTTTTGGGG CGATGACCGG TACGTCCCCA AGACCGATCC GGAACGCAAC GCCTGGCAAG CCTGGGAGGC ACTGCTGGAG CACGTGAACT TCCCACTGCG CAACATGCAT GCCATGCCCA ACAGCGAAAG CGAGTACGGC ACCGATCTTG ACGCCGCCGC ACTGGCATAC GAGCAGCTGT TGGCGGCCAA TGCCGAGCCC GGCCAGGATT GCCCGGCATT TGATGTCCAT CTGCTGGGCA TGGGCGGCGA AGGTCATATC AATTCATTGT TCCCGCATAC CGATGCCGTC AAGGAGACGC AGCGGCTCGT CGTGGCGGTG CCCGACTCTC CCAAGCCTCC GCCACAACGG ATCACCTTGA CCCTGCCGGC AATTCAGCGC TCACGCGAAG TGTGGCTGGT GGTTTCCGGC GAGGCGAAGG CCGATGCAGT GGCTGCGGCC GTCGGCGGTG CCGACCCTGT CGATGTGCCC GCGGCCGGCG CCAAGGGCAT CGAGCGCACG GTGTGGCTGC TCGATGAGGC CGCGGCAAGC CAACTGGGCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA