MytuD.01156.a

O-acetylhomoserine sulfhydrylase MetC

CENTER ID: MytuD.01156.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01156.a.A1.PS00164b Full length( MytuD.01156.a ) 1 449

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3732
RefSeq: NP_217857.1
TubercuList: Rv3340
MTB Network Portal: Rv3340
UniProt: O53390

Orthologues for Rv3340

Species % Identity % Coverage Clones Proteins Structures
marinum 91 100 1 1 1
ulcerans 91 100 0 0 0
avium 89 100 1 1 0
paratuberculosis 89 100 1 1 0
smegmatis 84 98 0 2 0
thermoresistibile 82 100 0 0 0
abscessus 76 100 1 1 0
smegmatis 50 93 1 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSADSNSTDA DPTAHWSFET KQIHAGQHPD PTTNARALPI YATTSYTFDD TAHAAALFGL EIPGNIYTRI GNPTTDVVEQ RIAALEGGVA ALFLSSGQAA ETFAILNLAG AGDHIVSSPR LYGGTYNLFH YSLAKLGIEV SFVDDPDDLD TWQAAVRPNT KAFFAETISN PQIDLLDTPA VSEVAHRNGV PLIVDNTIAT PYLIQPLAQG ADIVVHSATK YLGGHGAAIA GVIVDGGNFD WTQGRFPGFT TPDPSYHGVV FAELGPPAFA LKARVQLLRD YGSAASPFNA FLVAQGLETL SLRIERHVAN AQRVAEFLAA RDDVLSVNYA GLPSSPWHER AKRLAPKGTG AVLSFELAGG IEAGKAFVNA LKLHSHVANI GDVRSLVIHP ASTTHAQLSP AEQLATGVSP GLVRLAVGIE GIDDILADLE LGFAAARRFS ADPQSVAAF
NT Sequence
ATGAGCGCCG ACAGCAATAG CACCGACGCC GATCCGACCG CGCATTGGTC GTTCGAAACC AAACAGATAC ACGCTGGTCA GCACCCTGAT CCGACCACCA ACGCCCGGGC TCTGCCGATC TATGCGACCA CGTCGTACAC CTTCGACGAC ACCGCGCACG CCGCCGCCCT GTTCGGACTG GAAATTCCGG GCAATATCTA CACCCGGATC GGCAACCCCA CCACCGACGT CGTCGAGCAG CGCATCGCCG CGCTCGAGGG CGGTGTGGCC GCGCTGTTCC TGTCGTCGGG GCAGGCCGCG GAGACGTTCG CCATCTTGAA CCTGGCCGGC GCGGGCGATC ACATCGTGTC CAGCCCGCGC CTGTACGGCG GCACCTACAA CCTGTTCCAC TATTCGCTGG CCAAGCTCGG CATCGAGGTC AGCTTCGTCG ACGATCCGGA CGATCTGGAC ACCTGGCAGG CGGCGGTACG GCCCAACACC AAGGCGTTCT TCGCCGAGAC CATCTCCAAC CCGCAGATCG ACCTGCTGGA CACCCCGGCG GTTTCCGAGG TCGCCCATCG CAACGGGGTG CCGTTGATCG TCGACAACAC CATCGCCACG CCATACCTGA TCCAACCGTT GGCCCAGGGC GCCGACATCG TCGTGCATTC GGCCACCAAG TACCTGGGCG GGCACGGTGC CGCCATCGCG GGTGTGATCG TCGACGGCGG CAACTTCGAT TGGACCCAGG GCCGCTTCCC CGGCTTCACC ACCCCCGACC CCAGCTACCA CGGCGTGGTG TTCGCCGAGC TGGGTCCACC GGCGTTTGCG CTCAAAGCTC GAGTGCAGCT GCTCCGTGAC TACGGCTCGG CGGCTTCGCC GTTCAACGCG TTCTTGGTGG CGCAGGGTCT GGAAACGCTG AGCCTGCGGA TCGAGCGGCA CGTCGCCAAC GCGCAGCGCG TCGCCGAGTT CCTGGCCGCC CGCGACGACG TGCTTTCGGT CAACTATGCG GGGCTGCCCT CCTCGCCCTG GCATGAGCGG GCCAAGAGGC TGGCGCCCAA GGGAACCGGG GCCGTGCTGT CCTTCGAGTT GGCCGGCGGC ATCGAGGCCG GCAAGGCATT CGTGAACGCG TTGAAGCTGC ACAGCCACGT CGCCAACATC GGTGACGTGC GCTCGCTGGT GATCCACCCG GCATCGACCA CTCATGCCCA GCTGAGCCCG GCCGAGCAGC TGGCGACCGG GGTCAGCCCG GGCCTGGTGC GTTTGGCTGT GGGCATCGAA GGTATCGACG ATATCCTGGC CGACCTGGAG CTTGGCTTTG CCGCGGCCCG CAGATTCAGC GCCGACCCGC AGTCCGTGGC GGCGTTC
Details for MytuD.01156.a.A1.PS00164b
PURIFICATION DATe: 2/17/2009
CONCENTRATION: 32.31mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 74
PERCENT COVERAGE: 74
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSADSNS TDADPTAHWS FETKQIHAGQ HPDPTTNARA LPIYATTSYT FDDTAHAAAL FGLEIPGNIY TRIGNPTTDV VEQRIAALEG GVAALFLSSG QAAXTFAILN LAGAGDHIVS SPRLYGGTYN LFHYSLAKLG IEVSFVDDPD DLDTWQAAVR PNTKAFFAET ISXPXIDLLD TPAVSEVAHR NGVXXXVDNT IATPYLXXPL XXGAXXVVHS XTXYLGGHGX AIAGVXVXGG XFXXXQGRXP GFXXPXPXYX GXXXXRAGST GXCXXSXXAX XXXXXXXGXX VQRVXGXAGS GNAXXXDRAA XXXRAARRRV PGRPRRRAFG QLCGAALLXX A
Validated NT Sequence
ttagaacgcc gccacggact gcgggtcggc gctgaatctg cgggccgcgg caaagccaag ctccaggtcg gccaggatat cgtcgatacc ttcgatgccc acagccaaac gcaccaggcc cgggctgacc ccggtcgcca gctgctcggc cgggctcagc tgggcatgag tggtcgatgc cgggtggatc accagcgagc gcacgtcacc gatgttggcg acgtggctgt gcagcttcaa cgcgttcacg aatgccttgc cggcctcgat gccgccggcc aactcgaagg acagcacggc cccggttccc ttgggcgcca gcctcttggc ccgctcatgc canggngagg agggcagccc cgcatagttg accgaaagca cgtcgtcgcg ggcggccagg aactcggcga cgcgctgcgc gttnnnnncn ngccgctcga tccnnnnnnt cagcgtttcc ngaccctgcg cnnccaanna cgcgttgaac gnnnaagccg nnnnnncntn nncncnnnnn nncngcnnnc nagcttnnnn cgcannnncc ggtggaccca gctcggnnna ncnccncncc nnngtagcng ggntcggggn tggngaancc gggnaagcgn ccctggnncn natngaagtn gccgccgtng acgntcacac ccgcgatggc gncnccgtgn ccgcccaggt anttggtgnc ngaatgcacg acganntngg cgcccnggnc caacggtngg nncaggtatg gcgtggcgat ggtgttgtcg acgancancg ncaccccgtt gcgatgggcg acctcggaaa ccgccggggt gtccagcagg tcgatntgcg ggtnggagat ggtctcggcg aagaacgcct tggtgttggg ccgtaccgcc gcctgccagg tgtccagatc gtccggatcg tcgacgaagc tgacctcgat gccgagcttg gccagcgaat agtggaacag gttgtaggtg ccgccgtaca ggcgcgggct ggacacgatg tgatcgcccg cgccggccag gttcaagatg gcgaacgtnt ccgcggcctg ccccgacgac aggaacagcg cggccacacc gccctcgagc gcggcgatgc gctgctcgac gacgtcggtg gtggggttgc cgatccgggt gtagatattg cccggaattt ccagtccgaa cagggcggcg gcgtgcgcgg tgtcgtcgaa ggtgtacgac gtggtcgcat agatcggcag agcccgggcg ttggtggtcg gatcagggtg ctgaccagcg tgtatctgtt tggtttcgaa cgaccaatgc gcggtcggat cggcgtcggt gctattgctg tcggcgctca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatnag cca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSADSNSTD ADPTAHWSFE TKQIHAGQHP DPTTNARALP IYATTSYTFD DTAHAAALFG LEIPGNIYTR IGNPTTDVVE QRIAALEGGV AALFLSSGQA AETFAILNLA GAGDHIVSSP RLYGGTYNLF HYSLAKLGIE VSFVDDPDDL DTWQAAVRPN TKAFFAETIS NPQIDLLDTP AVSEVAHRNG VPLIVDNTIA TPYLIQPLAQ GADIVVHSAT KYLGGHGAAI AGVIVDGGNF DWTQGRFPGF TTPDPSYHGV VFAELGPPAF ALKARVQLLR DYGSAASPFN AFLVAQGLET LSLRIERHVA NAQRVAEFLA ARDDVLSVNY AGLPSSPWHE RAKRLAPKGT GAVLSFELAG GIEAGKAFVN ALKLHSHVAN IGDVRSLVIH PASTTHAQLS PAEQLATGVS PGLVRLAVGI EGIDDILADL ELGFAAARRF SADPQSVAAF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGCCGAC AGCAATAGCA CCGACGCCGA TCCGACCGCG CATTGGTCGT TCGAAACCAA ACAGATACAC GCTGGTCAGC ACCCTGATCC GACCACCAAC GCCCGGGCTC TGCCGATCTA TGCGACCACG TCGTACACCT TCGACGACAC CGCGCACGCC GCCGCCCTGT TCGGACTGGA AATTCCGGGC AATATCTACA CCCGGATCGG CAACCCCACC ACCGACGTCG TCGAGCAGCG CATCGCCGCG CTCGAGGGCG GTGTGGCCGC GCTGTTCCTG TCGTCGGGGC AGGCCGCGGA GACGTTCGCC ATCTTGAACC TGGCCGGCGC GGGCGATCAC ATCGTGTCCA GCCCGCGCCT GTACGGCGGC ACCTACAACC TGTTCCACTA TTCGCTGGCC AAGCTCGGCA TCGAGGTCAG CTTCGTCGAC GATCCGGACG ATCTGGACAC CTGGCAGGCG GCGGTACGGC CCAACACCAA GGCGTTCTTC GCCGAGACCA TCTCCAACCC GCAGATCGAC CTGCTGGACA CCCCGGCGGT TTCCGAGGTC GCCCATCGCA ACGGGGTGCC GTTGATCGTC GACAACACCA TCGCCACGCC ATACCTGATC CAACCGTTGG CCCAGGGCGC CGACATCGTC GTGCATTCGG CCACCAAGTA CCTGGGCGGG CACGGTGCCG CCATCGCGGG TGTGATCGTC GACGGCGGCA ACTTCGATTG GACCCAGGGC CGCTTCCCCG GCTTCACCAC CCCCGACCCC AGCTACCACG GCGTGGTGTT CGCCGAGCTG GGTCCACCGG CGTTTGCGCT CAAAGCTCGA GTGCAGCTGC TCCGTGACTA CGGCTCGGCG GCTTCGCCGT TCAACGCGTT CTTGGTGGCG CAGGGTCTGG AAACGCTGAG CCTGCGGATC GAGCGGCACG TCGCCAACGC GCAGCGCGTC GCCGAGTTCC TGGCCGCCCG CGACGACGTG CTTTCGGTCA ACTATGCGGG GCTGCCCTCC TCGCCCTGGC ATGAGCGGGC CAAGAGGCTG GCGCCCAAGG GAACCGGGGC CGTGCTGTCC TTCGAGTTGG CCGGCGGCAT CGAGGCCGGC AAGGCATTCG TGAACGCGTT GAAGCTGCAC AGCCACGTCG CCAACATCGG TGACGTGCGC TCGCTGGTGA TCCACCCGGC ATCGACCACT CATGCCCAGC TGAGCCCGGC CGAGCAGCTG GCGACCGGGG TCAGCCCGGG CCTGGTGCGT TTGGCTGTGG GCATCGAAGG TATCGACGAT ATCCTGGCCG ACCTGGAGCT TGGCTTTGCC GCGGCCCGCA GATTCAGCGC CGACCCGCAG TCCGTGGCGG CGTTCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA