MysmA.01156.a

O-acetylhomoserine/O-acetylserine sulfhydrylase

CENTER ID: MysmA.01156.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01156.a.A1.GE28883 Full length( MysmA.01156.a ) 1 431
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01156.a.A1.PW28788 Full length( MysmA.01156.a ) 1 431

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.235
RefSeq: YP_884654.1
UniProt: A0QP16

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDRTFGLAT RAIHAGQRPD AATGARVTPI YANASFVFND TDDAANLFAL QKYGNIYSRI GNPTVAAFEE CIASLENGLG AVAMASGLSA QFAVFAALAG AGDHIVSAAS LYGGTVTQLD VSLRRFGIDT TFVGGTDPAD YAAAVTDRTR LIYAEVISNP SGEIADLAGL AEVARDAGVP LIVDSTVATP ALCRPIEHGA DIVVHSATKF IGGHGTTLGG VAVDAGRFDW GAGKFPQMTE PVASYGGLSW WGNFGEFGFL TKLRSEQLRD LGATLAPQSA FQLLQGVQTL PQRMAAHIAN ARAVAEWLEA DPRVTYVRWA GLPSHPHHDR ARHYLPDGPG AVFAFGVKGG RAAGEKFINS VQLASHLANI GDVRTLVVHP ASTTHRQLTD EQLTAGGVGP ELIRISVGIE DVEDIIWDLD QALTQAEKAD D
NT Sequence
GTGACTGACC GAACCTTCGG CCTGGCCACC CGCGCCATAC ACGCCGGACA ACGTCCGGAT GCTGCCACCG GTGCACGGGT GACGCCGATC TACGCCAACG CCAGCTTCGT CTTCAACGAC ACCGACGACG CGGCCAACCT GTTCGCACTG CAGAAGTACG GCAACATCTA CAGCCGGATC GGCAACCCCA CCGTCGCAGC CTTCGAAGAA TGCATCGCAA GCCTGGAGAA CGGGCTGGGT GCGGTGGCCA TGGCCAGCGG TCTGTCCGCG CAGTTCGCGG TGTTCGCCGC GCTCGCGGGC GCAGGCGACC ACATCGTGTC GGCCGCGAGC CTCTACGGCG GCACCGTCAC GCAGCTCGAC GTCTCGCTGC GCCGGTTCGG CATCGACACC ACGTTCGTGG GCGGCACCGA CCCGGCCGAC TACGCCGCAG CGGTCACCGA CCGCACCCGG CTGATCTACG CCGAGGTGAT CTCCAATCCG TCCGGTGAGA TCGCCGACCT CGCAGGTCTG GCCGAGGTCG CCAGGGACGC CGGGGTTCCG CTGATCGTCG ACAGCACGGT CGCCACGCCT GCCCTGTGCC GTCCGATCGA GCACGGCGCG GACATCGTCG TGCACTCGGC CACCAAGTTC ATCGGCGGGC ACGGCACCAC GCTCGGCGGC GTCGCGGTCG ACGCGGGCCG ATTCGACTGG GGCGCAGGCA AATTCCCGCA GATGACCGAG CCTGTCGCGT CCTACGGCGG GCTGAGCTGG TGGGGCAACT TCGGCGAGTT CGGGTTCCTG ACCAAGCTGC GCAGCGAGCA GCTGCGTGAT CTGGGTGCCA CGCTGGCCCC GCAGTCGGCC TTCCAGCTCC TACAGGGCGT GCAGACGCTA CCGCAGCGCA TGGCCGCCCA CATCGCCAAC GCGCGCGCGG TCGCCGAGTG GCTCGAGGCC GATCCGCGCG TGACGTACGT CCGCTGGGCC GGGCTGCCGT CGCATCCGCA TCACGACCGG GCCAGGCACT ACCTGCCCGA CGGCCCCGGC GCGGTGTTCG CGTTCGGCGT CAAGGGTGGC CGCGCCGCGG GCGAGAAGTT CATCAACTCC GTGCAACTCG CGAGCCACCT GGCCAACATC GGCGACGTGC GCACGCTCGT GGTGCACCCC GCCAGCACGA CACACCGCCA GCTCACCGAC GAGCAGCTCA CCGCGGGCGG CGTCGGCCCC GAGCTCATCC GCATCAGCGT GGGCATCGAG GACGTCGAGG ACATCATCTG GGATCTCGAC CAGGCACTGA CACAAGCGGA GAAAGCCGAT GAC
Details for MysmA.01156.a.A1.GE28883
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 42
Validated AA Sequence
MTEPVASYGG LSWWGNFGEF GFLTKLRSEQ LRDLGATLAP QSAFQLLQGV QTLPQRMAAH IANARAVAEW LEADPRVTYV RWAGLPSHPH HDRARHYLPD GPGAVFAFGV KGGRAAGEKF INSVQLASHL ANIGDVRTLV VHPASTTHRQ LTDEQLTAGG VGPELIRISV GIEDVEDIIW DLDQALTQAE KA
Validated NT Sequence
cggctttctc cgcttgtgtc agtgcctggt cgagatccca gatgatgtcc tcgacgtcct cgatgcccac gctgatgcgg atgagctcgg ggccgacgcc gcccgcggtg agctgctcgt cggtgagctg gcggtgtgtc gtgctggcgg ggtgcaccac gagcgtgcgc acgtcgccga tgttggccag gtggctcgcg agttgcacgg agttgatgaa cttctcgccc gcggcgcggc cacccttgac gccgaacgcg aacaccgcgc cggggccgtc gggcaggtag tgcctggccc ggtcgtgatg cggatgcgac ggcagcccgg cccagcggac gtacgtcacg cgcggatcgg cctcgagcca ctcggcgacc gcgcgcgcgt tggcgatgtg ggcggccatg cgctgcggta gcgtctgcac gccctgtagg agctggaagg ccgactgcgg ggccagcgtg gcacccagat cacgcagctg ctcgctgcgc agcttggtca ggaacccgaa ctcgccgaag ttgccccacc agctcagccc gccgtaggac gcgacaggct cggtcatctg cgggaatttg cctgcgcccc agtcgaatcg gcccgcgtcg accgcgacgc cgccgagcgt ggtgccgtgc ccgccgatga acttggtggc cgagtgcacg acgatgtccg cgccgtgctc gatcggacgg cacagggcan gcgtggcgac cgtgctgtcg acgatcagcg gaaccccggc gtccctggcg acctcggcca gacctgcgag gncggcgatc tcaccggacg gattgganat cacctcggcg tanatcagcc gggtgcggtc ggtgaccgct gcggcgta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDRTFGL ATRAIHAGQR PDAATGARVT PIYANASFVF NDTDDAANLF ALQKYGNIYS RIGNPTVAAF EECIASLENG LGAVAMASGL SAQFAVFAAL AGAGDHIVSA ASLYGGTVTQ LDVSLRRFGI DTTFVGGTDP ADYAAAVTDR TRLIYAEVIS NPSGEIADLA GLAEVARDAG VPLIVDSTVA TPALCRPIEH GADIVVHSAT KFIGGHGTTL GGVAVDAGRF DWGAGKFPQM TEPVASYGGL SWWGNFGEFG FLTKLRSEQL RDLGATLAPQ SAFQLLQGVQ TLPQRMAAHI ANARAVAEWL EADPRVTYVR WAGLPSHPHH DRARHYLPDG PGAVFAFGVK GGRAAGEKFI NSVQLASHLA NIGDVRTLVV HPASTTHRQL TDEQLTAGGV GPELIRISVG IEDVEDIIWD LDQALTQAEK ADD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactgac cgaaccttcg gcctggccac ccgcgccata cacgccggac aacgtccgga tgctgccacc ggtgcacggg tgacgccgat ctacgccaac gccagcttcg tcttcaacga caccgacgac gcggccaacc tgttcgcact gcagaagtac ggcaacatct acagccggat cggcaacccc accgtcgcag ccttcgaaga atgcatcgca agcctggaga acgggctggg tgcggtggcc atggccagcg gtctgtccgc gcagttcgcg gtgttcgccg cgctcgcggg cgcaggcgac cacatcgtgt cggccgcgag cctctacggc ggcaccgtca cgcagctcga cgtctcgctg cgccggttcg gcatcgacac cacgttcgtg ggcggcaccg acccggccga ctacgccgca gcggtcaccg accgcacccg gctgatctac gccgaggtga tctccaatcc gtccggtgag atcgccgacc tcgcaggtct ggccgaggtc gccagggacg ccggggttcc gctgatcgtc gacagcacgg tcgccacgcc tgccctgtgc cgtccgatcg agcacggcgc ggacatcgtc gtgcactcgg ccaccaagtt catcggcggg cacggcacca cgctcggcgg cgtcgcggtc gacgcgggcc gattcgactg gggcgcaggc aaattcccgc agatgaccga gcctgtcgcg tcctacggcg ggctgagctg gtggggcaac ttcggcgagt tcgggttcct gaccaagctg cgcagcgagc agctgcgtga tctgggtgcc acgctggccc cgcagtcggc cttccagctc ctacagggcg tgcagacgct accgcagcgc atggccgccc acatcgccaa cgcgcgcgcg gtcgccgagt ggctcgaggc cgatccgcgc gtgacgtacg tccgctgggc cgggctgccg tcgcatccgc atcacgaccg ggccaggcac tacctgcccg acggccccgg cgcggtgttc gcgttcggcg tcaagggtgg ccgcgccgcg ggcgagaagt tcatcaactc cgtgcaactc gcgagccacc tggccaacat cggcgacgtg cgcacgctcg tggtgcaccc cgccagcacg acacaccgcc agctcaccga cgagcagctc accgcgggcg gcgtcggccc cgagctcatc cgcatcagcg tgggcatcga ggacgtcgag gacatcatct gggatctcga ccaggcactg acacaagcgg agaaagccga tgacaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MysmA.01156.a.A1.PW28788
PURIFICATION DATe: 6/1/2010
CONCENTRATION: 24.38mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 42
Protocol Notes
notes unavailable
Validated AA Sequence
MTEPVASYGG LSWWGNFGEF GFLTKLRSEQ LRDLGATLAP QSAFQLLQGV QTLPQRMAAH IANARAVAEW LEADPRVTYV RWAGLPSHPH HDRARHYLPD GPGAVFAFGV KGGRAAGEKF INSVQLASHL ANIGDVRTLV VHPASTTHRQ LTDEQLTAGG VGPELIRISV GIEDVEDIIW DLDQALTQAE KA
Validated NT Sequence
cggctttctc cgcttgtgtc agtgcctggt cgagatccca gatgatgtcc tcgacgtcct cgatgcccac gctgatgcgg atgagctcgg ggccgacgcc gcccgcggtg agctgctcgt cggtgagctg gcggtgtgtc gtgctggcgg ggtgcaccac gagcgtgcgc acgtcgccga tgttggccag gtggctcgcg agttgcacgg agttgatgaa cttctcgccc gcggcgcggc cacccttgac gccgaacgcg aacaccgcgc cggggccgtc gggcaggtag tgcctggccc ggtcgtgatg cggatgcgac ggcagcccgg cccagcggac gtacgtcacg cgcggatcgg cctcgagcca ctcggcgacc gcgcgcgcgt tggcgatgtg ggcggccatg cgctgcggta gcgtctgcac gccctgtagg agctggaagg ccgactgcgg ggccagcgtg gcacccagat cacgcagctg ctcgctgcgc agcttggtca ggaacccgaa ctcgccgaag ttgccccacc agctcagccc gccgtaggac gcgacaggct cggtcatctg cgggaatttg cctgcgcccc agtcgaatcg gcccgcgtcg accgcgacgc cgccgagcgt ggtgccgtgc ccgccgatga acttggtggc cgagtgcacg acgatgtccg cgccgtgctc gatcggacgg cacagggcan gcgtggcgac cgtgctgtcg acgatcagcg gaaccccggc gtccctggcg acctcggcca gacctgcgag gncggcgatc tcaccggacg gattgganat cacctcggcg tanatcagcc gggtgcggtc ggtgaccgct gcggcgta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDRTFGL ATRAIHAGQR PDAATGARVT PIYANASFVF NDTDDAANLF ALQKYGNIYS RIGNPTVAAF EECIASLENG LGAVAMASGL SAQFAVFAAL AGAGDHIVSA ASLYGGTVTQ LDVSLRRFGI DTTFVGGTDP ADYAAAVTDR TRLIYAEVIS NPSGEIADLA GLAEVARDAG VPLIVDSTVA TPALCRPIEH GADIVVHSAT KFIGGHGTTL GGVAVDAGRF DWGAGKFPQM TEPVASYGGL SWWGNFGEFG FLTKLRSEQL RDLGATLAPQ SAFQLLQGVQ TLPQRMAAHI ANARAVAEWL EADPRVTYVR WAGLPSHPHH DRARHYLPDG PGAVFAFGVK GGRAAGEKFI NSVQLASHLA NIGDVRTLVV HPASTTHRQL TDEQLTAGGV GPELIRISVG IEDVEDIIWD LDQALTQAEK ADD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTGAC CGAACCTTCG GCCTGGCCAC CCGCGCCATA CACGCCGGAC AACGTCCGGA TGCTGCCACC GGTGCACGGG TGACGCCGAT CTACGCCAAC GCCAGCTTCG TCTTCAACGA CACCGACGAC GCGGCCAACC TGTTCGCACT GCAGAAGTAC GGCAACATCT ACAGCCGGAT CGGCAACCCC ACCGTCGCAG CCTTCGAAGA ATGCATCGCA AGCCTGGAGA ACGGGCTGGG TGCGGTGGCC ATGGCCAGCG GTCTGTCCGC GCAGTTCGCG GTGTTCGCCG CGCTCGCGGG CGCAGGCGAC CACATCGTGT CGGCCGCGAG CCTCTACGGC GGCACCGTCA CGCAGCTCGA CGTCTCGCTG CGCCGGTTCG GCATCGACAC CACGTTCGTG GGCGGCACCG ACCCGGCCGA CTACGCCGCA GCGGTCACCG ACCGCACCCG GCTGATCTAC GCCGAGGTGA TCTCCAATCC GTCCGGTGAG ATCGCCGACC TCGCAGGTCT GGCCGAGGTC GCCAGGGACG CCGGGGTTCC GCTGATCGTC GACAGCACGG TCGCCACGCC TGCCCTGTGC CGTCCGATCG AGCACGGCGC GGACATCGTC GTGCACTCGG CCACCAAGTT CATCGGCGGG CACGGCACCA CGCTCGGCGG CGTCGCGGTC GACGCGGGCC GATTCGACTG GGGCGCAGGC AAATTCCCGC AGATGACCGA GCCTGTCGCG TCCTACGGCG GGCTGAGCTG GTGGGGCAAC TTCGGCGAGT TCGGGTTCCT GACCAAGCTG CGCAGCGAGC AGCTGCGTGA TCTGGGTGCC ACGCTGGCCC CGCAGTCGGC CTTCCAGCTC CTACAGGGCG TGCAGACGCT ACCGCAGCGC ATGGCCGCCC ACATCGCCAA CGCGCGCGCG GTCGCCGAGT GGCTCGAGGC CGATCCGCGC GTGACGTACG TCCGCTGGGC CGGGCTGCCG TCGCATCCGC ATCACGACCG GGCCAGGCAC TACCTGCCCG ACGGCCCCGG CGCGGTGTTC GCGTTCGGCG TCAAGGGTGG CCGCGCCGCG GGCGAGAAGT TCATCAACTC CGTGCAACTC GCGAGCCACC TGGCCAACAT CGGCGACGTG CGCACGCTCG TGGTGCACCC CGCCAGCACG ACACACCGCC AGCTCACCGA CGAGCAGCTC ACCGCGGGCG GCGTCGGCCC CGAGCTCATC CGCATCAGCG TGGGCATCGA GGACGTCGAG GACATCATCT GGGATCTCGA CCAGGCACTG ACACAAGCGG AGAAAGCCGA TGACAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA