SSGCID: Target Page

MypaA.01156.a

MetC

CENTER ID:	MypaA.01156.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01156.a.A1.GE29556	Full length( MypaA.01156.a )		1	456

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01156.a.A1.PW29454	Full length( MypaA.01156.a )		1	456

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.3675
RefSeq:	NP_962391.1
UniProt:	Q73UB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSENTDHDT DPSAHWSFET KQVHAGQHPD SATNARALPI YQTTSYTFDD TTHAAALFGL EVPGNIYTRI GNPTTDVVEQ RIAALEGGVA ALFLSSGQAA ETFAILNLAS AGDHIVSSPR LYGGTYNLFH YSLAKLGIEV SFVEDPDDLD SWQAAVRPNT KAFFAETISN PQIDILDIPG VSGVAHANGV PLIVDNTIAT PYLIQPFAHG ADIVVHSATK YLGGHGSAIA GVIVDGGTFD WTQGRFPGFT TPDPSYHGVV FAELGPPAYA LKARVQLLRD LGSAAAPFNA FLVAQGIETL SLRMERHVAN AQRVAEFLAS HDGVLSVNYA GLPGSPWHER AKKLAPKGTG AVLSFELAGG VEAGKAFVNA LKLHSHVANI GDVRSLVIHP ASTTHAQLSP AEQLSTGVSP GLVRLAVGIE GIDDILADLE LGFAAAAKFG ANADTSGADP HAVAAF

NT Sequence

GTGAGCTCCG AGAACACCGA TCACGACACC GACCCCAGCG CGCACTGGTC GTTCGAGACC AAGCAGGTGC ACGCCGGCCA GCACCCCGAC TCGGCCACCA ACGCCCGGGC CCTGCCGATC TACCAGACCA CGTCGTACAC CTTCGACGAC ACCACCCACG CCGCCGCCCT GTTCGGGCTG GAGGTGCCGG GCAACATCTA CACCCGGATC GGCAACCCGA CCACCGACGT CGTCGAGCAG CGCATCGCCG CCCTGGAGGG CGGGGTGGCG GCGCTGTTTC TGAGCTCCGG TCAGGCCGCT GAGACTTTCG CCATATTGAA CCTGGCAAGT GCCGGAGATC ACATCGTCTC CAGCCCGCGG CTCTACGGCG GCACCTACAA CCTGTTCCAC TATTCGCTGG CCAAGCTCGG TATCGAGGTC AGCTTCGTGG AGGATCCCGA CGACCTGGAT TCCTGGCAGG CCGCGGTCCG GCCGAACACC AAGGCGTTCT TCGCCGAGAC CATCTCCAAC CCGCAGATCG ACATCCTGGA CATCCCTGGC GTCTCGGGCG TCGCCCACGC CAACGGCGTG CCGCTGATCG TCGACAACAC CATCGCCACG CCGTACCTGA TCCAGCCGTT CGCGCACGGC GCCGACATCG TGGTGCACTC GGCCACCAAG TACCTCGGCG GTCACGGCTC GGCGATCGCC GGCGTGATCG TCGACGGCGG CACCTTCGAC TGGACGCAGG GCCGGTTCCC GGGCTTCACC ACCCCCGACC CGAGCTACCA CGGCGTGGTG TTCGCGGAGC TGGGGCCGCC GGCGTACGCG CTCAAGGCCC GCGTGCAGTT GCTGCGCGAC CTCGGCTCGG CCGCGGCGCC GTTCAACGCG TTCCTGGTCG CCCAGGGCAT CGAGACGCTG AGCCTGCGCA TGGAGCGGCA CGTCGCCAAC GCGCAGCGGG TGGCCGAGTT CCTGGCCTCT CATGACGGCG TGCTGTCGGT GAACTACGCG GGACTGCCCG GCTCGCCGTG GCACGAGCGG GCAAAGAAGC TGGCGCCCAA GGGAACCGGG GCGGTGCTGT CGTTCGAGTT GGCCGGCGGG GTGGAGGCCG GCAAGGCGTT CGTGAACGCG CTGAAGCTGC ACAGCCACGT CGCCAACATC GGCGACGTCC GCTCGCTGGT CATCCACCCG GCCTCGACCA CGCACGCCCA ACTGTCCCCG GCCGAGCAGC TGAGCACCGG GGTCAGCCCG GGCCTGGTGC GGCTGGCGGT CGGCATCGAG GGAATCGACG ACATCCTGGC CGACCTGGAG CTCGGTTTCG CCGCGGCCGC CAAATTCGGT GCGAACGCGG ACACCTCCGG CGCCGACCCG CACGCCGTGG CGGCGTTC

Details for MypaA.01156.a.A1.GE29556

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	50kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	25

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSSENTDH DTDPSAHWSF ETKQVHAGQH PDSATNARAL PIYQTTSYTF DDTTHAAALF GLEVPGNIYT RIGNPTTDVV EQRIAALEGG VAALFLSSGQ AAEX

Validated NT Sequence

ttttgtttnn ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gctccgagaa caccgatcac gacaccgacc ccagcgcgca ctggtcgttc gagaccaagc aggtgcacgc cggccagcac cccgactcgg ccaccaacgc ccgggccctg ccgatctacc agaccacgtc gtacaccttc gacgacacca cccacgccgc cgccctgttc gggctggagg tgccgggcaa catctacacc cggatcggca acccgaccac cgacgtcgtc gagcagcgca tcgccgccct ggagggcggg gtggcggcgc tgtttctgag ctccggtcag gccgctgagc antaactagc aannnncctg gnnnngncga gatcacntcg ncnccagccc gngtctntnc ggcgnnncct ncnnccngnc nncnnntcgn nngnnnanct cgntatnnan nnctgnttnn ngnnnnntnc cnncnacctn nnnncnngnn nngnnnngnt tcnnnnnnaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSSENTDH DTDPSAHWSF ETKQVHAGQH PDSATNARAL PIYQTTSYTF DDTTHAAALF GLEVPGNIYT RIGNPTTDVV EQRIAALEGG VAALFLSSGQ AAETFAILNL ASAGDHIVSS PRLYGGTYNL FHYSLAKLGI EVSFVEDPDD LDSWQAAVRP NTKAFFAETI SNPQIDILDI PGVSGVAHAN GVPLIVDNTI ATPYLIQPFA HGADIVVHSA TKYLGGHGSA IAGVIVDGGT FDWTQGRFPG FTTPDPSYHG VVFAELGPPA YALKARVQLL RDLGSAAAPF NAFLVAQGIE TLSLRMERHV ANAQRVAEFL ASHDGVLSVN YAGLPGSPWH ERAKKLAPKG TGAVLSFELA GGVEAGKAFV NALKLHSHVA NIGDVRSLVI HPASTTHAQL SPAEQLSTGV SPGLVRLAVG IEGIDDILAD LELGFAAAAK FGANADTSGA DPHAVAAF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagctcc gagaacaccg atcacgacac cgaccccagc gcgcactggt cgttcgagac caagcaggtg cacgccggcc agcaccccga ctcggccacc aacgcccggg ccctgccgat ctaccagacc acgtcgtaca ccttcgacga caccacccac gccgccgccc tgttcgggct ggaggtgccg ggcaacatct acacccggat cggcaacccg accaccgacg tcgtcgagca gcgcatcgcc gccctggagg gcggggtggc ggcgctgttt ctgagctccg gtcaggccgc tgagactttc gccatattga acctggcaag tgccggagat cacatcgtct ccagcccgcg gctctacggc ggcacctaca acctgttcca ctattcgctg gccaagctcg gtatcgaggt cagcttcgtg gaggatcccg acgacctgga ttcctggcag gccgcggtcc ggccgaacac caaggcgttc ttcgccgaga ccatctccaa cccgcagatc gacatcctgg acatccctgg cgtctcgggc gtcgcccacg ccaacggcgt gccgctgatc gtcgacaaca ccatcgccac gccgtacctg atccagccgt tcgcgcacgg cgccgacatc gtggtgcact cggccaccaa gtacctcggc ggtcacggct cggcgatcgc cggcgtgatc gtcgacggcg gcaccttcga ctggacgcag ggccggttcc cgggcttcac cacccccgac ccgagctacc acggcgtggt gttcgcggag ctggggccgc cggcgtacgc gctcaaggcc cgcgtgcagt tgctgcgcga cctcggctcg gccgcggcgc cgttcaacgc gttcctggtc gcccagggca tcgagacgct gagcctgcgc atggagcggc acgtcgccaa cgcgcagcgg gtggccgagt tcctggcctc tcatgacggc gtgctgtcgg tgaactacgc gggactgccc ggctcgccgt ggcacgagcg ggcaaagaag ctggcgccca agggaaccgg ggcggtgctg tcgttcgagt tggccggcgg ggtggaggcc ggcaaggcgt tcgtgaacgc gctgaagctg cacagccacg tcgccaacat cggcgacgtc cgctcgctgg tcatccaccc ggcctcgacc acgcacgccc aactgtcccc ggccgagcag ctgagcaccg gggtcagccc gggcctggtg cggctggcgg tcggcatcga gggaatcgac gacatcctgg ccgacctgga gctcggtttc gccgcggccg ccaaattcgg tgcgaacgcg gacacctccg gcgccgaccc gcacgccgtg gcggcgttca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MypaA.01156.a.A1.PW29454

PURIFICATION DATe:	8/23/2010
CONCENTRATION:	21.95mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	15
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	25

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSSENTDH DTDPSAHWSF ETKQVHAGQH PDSATNARAL PIYQTTSYTF DDTTHAAALF GLEVPGNIYT RIGNPTTDVV EQRIAALEGG VAALFLSSGQ AAEX

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCTCC GAGAACACCG ATCACGACAC CGACCCCAGC GCGCACTGGT CGTTCGAGAC CAAGCAGGTG CACGCCGGCC AGCACCCCGA CTCGGCCACC AACGCCCGGG CCCTGCCGAT CTACCAGACC ACGTCGTACA CCTTCGACGA CACCACCCAC GCCGCCGCCC TGTTCGGGCT GGAGGTGCCG GGCAACATCT ACACCCGGAT CGGCAACCCG ACCACCGACG TCGTCGAGCA GCGCATCGCC GCCCTGGAGG GCGGGGTGGC GGCGCTGTTT CTGAGCTCCG GTCAGGCCGC TGAGACTTTC GCCATATTGA ACCTGGCAAG TGCCGGAGAT CACATCGTCT CCAGCCCGCG GCTCTACGGC GGCACCTACA ACCTGTTCCA CTATTCGCTG GCCAAGCTCG GTATCGAGGT CAGCTTCGTG GAGGATCCCG ACGACCTGGA TTCCTGGCAG GCCGCGGTCC GGCCGAACAC CAAGGCGTTC TTCGCCGAGA CCATCTCCAA CCCGCAGATC GACATCCTGG ACATCCCTGG CGTCTCGGGC GTCGCCCACG CCAACGGCGT GCCGCTGATC GTCGACAACA CCATCGCCAC GCCGTACCTG ATCCAGCCGT TCGCGCACGG CGCCGACATC GTGGTGCACT CGGCCACCAA GTACCTCGGC GGTCACGGCT CGGCGATCGC CGGCGTGATC GTCGACGGCG GCACCTTCGA CTGGACGCAG GGCCGGTTCC CGGGCTTCAC CACCCCCGAC CCGAGCTACC ACGGCGTGGT GTTCGCGGAG CTGGGGCCGC CGGCGTACGC GCTCAAGGCC CGCGTGCAGT TGCTGCGCGA CCTCGGCTCG GCCGCGGCGC CGTTCAACGC GTTCCTGGTC GCCCAGGGCA TCGAGACGCT GAGCCTGCGC ATGGAGCGGC ACGTCGCCAA CGCGCAGCGG GTGGCCGAGT TCCTGGCCTC TCATGACGGC GTGCTGTCGG TGAACTACGC GGGACTGCCC GGCTCGCCGT GGCACGAGCG GGCAAAGAAG CTGGCGCCCA AGGGAACCGG GGCGGTGCTG TCGTTCGAGT TGGCCGGCGG GGTGGAGGCC GGCAAGGCGT TCGTGAACGC GCTGAAGCTG CACAGCCACG TCGCCAACAT CGGCGACGTC CGCTCGCTGG TCATCCACCC GGCCTCGACC ACGCACGCCC AACTGTCCCC GGCCGAGCAG CTGAGCACCG GGGTCAGCCC GGGCCTGGTG CGGCTGGCGG TCGGCATCGA GGGAATCGAC GACATCCTGG CCGACCTGGA GCTCGGTTTC GCCGCGGCCG CCAAATTCGG TGCGAACGCG GACACCTCCG GCGCCGACCC GCACGCCGTG GCGGCGTTCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A