MytuD.00656.a

POSSIBLE RIBONUCLEOTIDE-TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER MKL

CENTER ID: MytuD.00656.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00656.a.B1.GE27166 Full length( MytuD.00656.a ) 1 359
MytuD.00656.a.B1.GE27587 Full length( MytuD.00656.a ) 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.730
RefSeq: NP_215169.1
TubercuList: Rv0655
MTB Network Portal: Rv0655
UniProt: P9WQL5

Orthologues for Rv0655

Species % Identity % Coverage Clones Proteins Structures
marinum 96 91 1 0 0
ulcerans 96 91 0 0 0
leprae 93 94 1 0 0
paratuberculosis 92 93 0 0 0
avium 89 93 0 0 0
smegmatis 88 90 0 0 0
abscessus 85 93 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRYSDSYHTT GRWQPRASTE GFPMGVSIEV NGLTKSFGSS RIWEDVTLTI PAGEVSVLLG PSGTGKSVFL KSLIGLLRPE RGSIIIDGTD IIECSAKELY EIRTLFGVLF QDGALFGSMN LYDNTAFPLR EHTKKKESEI RDIVMEKLAL VGLGGDEKKF PGEISGGMRK RAGLARALVL DPQIILCDEP DSGLDPVRTA YLSQLIMDIN AQIDATILIV THNINIARTV PDNMGMLFRK HLVMFGPREV LLTSDEPVVR QFLNGRRIGP IGMSEEKDEA TMAEEQALLD AGHHAGGVEE IEGVPPQISA TPGMPERKAV ARRQARVREM LHTLPKKAQA AILDDLEGTH KYAVHEIGQ
NT Sequence
GTGCGATACA GTGACTCATA CCACACAACG GGCCGGTGGC AGCCACGAGC GTCGACAGAA GGGTTTCCCA TGGGCGTCAG CATCGAGGTC AACGGACTAA CGAAGTCCTT CGGGTCCTCG AGGATCTGGG AAGATGTCAC GCTAACGATC CCCGCCGGGG AGGTCAGCGT GCTGCTGGGC CCATCGGGTA CCGGCAAATC GGTGTTTCTG AAATCTCTGA TCGGCCTCCT GCGGCCGGAG CGCGGCTCGA TCATCATCGA CGGCACCGAC ATCATCGAAT GCTCGGCCAA GGAGCTTTAC GAGATCCGCA CATTGTTCGG CGTGCTGTTT CAGGACGGTG CCCTGTTCGG GTCGATGAAC CTCTACGACA ACACCGCGTT CCCCCTGCGT GAGCACACCA AGAAAAAGGA AAGCGAGATC CGTGACATCG TCATGGAGAA GCTGGCCCTA GTCGGCCTGG GTGGGGACGA GAAGAAGTTC CCCGGCGAGA TCTCCGGCGG GATGCGTAAG CGTGCCGGCC TAGCGCGTGC CCTGGTCCTT GACCCGCAGA TCATTCTCTG CGACGAGCCC GACTCGGGTC TGGACCCGGT TCGTACCGCC TACCTGAGCC AGCTGATCAT GGACATCAAC GCCCAGATCG ACGCCACCAT CCTGATCGTG ACGCACAACA TCAACATCGC CCGCACCGTG CCGGACAACA TGGGCATGTT GTTCCGCAAG CATTTGGTGA TGTTCGGGCC GCGGGAGGTG CTACTCACCA GCGACGAGCC GGTGGTGCGG CAGTTCCTCA ACGGCCGGCG CATCGGCCCG ATCGGCATGT CCGAGGAGAA GGACGAGGCC ACCATGGCCG AAGAGCAGGC CCTGCTCGAT GCCGGCCACC ACGCGGGCGG TGTCGAGGAA ATCGAGGGCG TGCCGCCGCA GATCAGCGCG ACACCGGGCA TGCCGGAGCG CAAAGCGGTC GCCCGGCGTC AGGCTCGGGT TCGCGAGATG TTGCACACGC TGCCCAAAAA GGCCCAGGCG GCGATCCTCG ACGATCTCGA GGGCACGCAC AAGTACGCGG TGCACGAAAT CGGCCAG
Details for MytuD.00656.a.B1.GE27166
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 40kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV RYSDSYHTTG RWQPRASTEG FPMGVSIEVN GLTKSFGSSR IWEDVTLTIP AGEVSVLLGP SGTGKSVFLK SLIGLLRPER GSIIIDGTDI IECSAKELYE IRTLFGVLFQ DGALFGSMNL YDNTAFPLRE HTKKKESEIR DIVMEKLALV GLGGDEKKFP GEISGGMRKR AGLARALVLD PQIILCDEPD SGLDPVRTAY LSQLIMDINA QIDATILIVT HNINIARTVP DNMGMLFRKH LVMFGPREVL LTSDEPVVRQ FLNGRRIGPI GMSEEKDEAT MAEEQALLDA GHHAGGVEEI EGVPPQISAT PGMPERKAVA RRQARVREML HTLPKKAQAA ILDDLEGTHK YAVHEIGQ
Validated NT Sequence
cttactggcc gatttcgtgc accgcgtact tgtgcgtgcc ctcgagatcg tcgaggatcg ccgcctgggc ctttttgggc agcgtgtgca acatctcgcg aacccgagcc tgacgccggg cgaccgcttt gcgctccggc atgcccggtg tcgcgctgat ctgcggcggc acgccctcga tttcctcgac accgcccgcg tggtggccgg catcgagcag ggcctgctct tcggccatgg tggcctcgtc cttctcctcg gacatgccga tcgggccgat gcgccggccg ttgaggaact gccgcaccac cggctcgtcg ctggtgagta gcacctcccg cggcccgaac atcaccaaat gcttgcggaa caacatgccc atgttgtccg gcacggtgcg ggcgatgttg atgttgtgcg tcacgatcag gatggtggcg tcgatctggg cgttgatgtc catgatcagc tggctcaggt aggcggtacg aaccgggtcc agacccgagt cgggctcgtc gcagagaatg atctgcgggt caaggaccag ggcacgcgct aggccggcac gcttacgcat cccgccggag atctcgccgg ggaacttctt ctcgtcccca cccaggccga ctagggccag cttctccatg acgatgtcac ggatctcgct ttcctttttc ttggtgtgct cacgcagggg gaacgcggtg ttgtcgtaga ggttcatcga cccgaacagg gcaccgtcct gaaacagcac gccgaacaat gtgcggatct cgtaaagctc cttggccgag cattcgatga tgtcggtgcc gtcgatgatg atcgagccgc gctccggccg caggaggccg atcagagatt tcagaaacac cgatttgccg gtacccgatg ggcccagcag cacgctgacc tccccggcgg ggatcgttag cgtgacatct tcccagatcc tcgaggaccc gaaggacttc gttagtccgt tgacctcgat gctgacgccc atgggaaacc cttctgtcga cgctcgtggc tgccaccggc ccgttgtgtg gtatgagtca ctgtatcgca ccatatggtg gtggtggtgg tgagccatgg tatnnctcct t
Expected Protein Sequence
MAHHHHHHMV RYSDSYHTTG RWQPRASTEG FPMGVSIEVN GLTKSFGSSR IWEDVTLTIP AGEVSVLLGP SGTGKSVFLK SLIGLLRPER GSIIIDGTDI IECSAKELYE IRTLFGVLFQ DGALFGSMNL YDNTAFPLRE HTKKKESEIR DIVMEKLALV GLGGDEKKFP GEISGGMRKR AGLARALVLD PQIILCDEPD SGLDPVRTAY LSQLIMDINA QIDATILIVT HNINIARTVP DNMGMLFRKH LVMFGPREVL LTSDEPVVRQ FLNGRRIGPI GMSEEKDEAT MAEEQALLDA GHHAGGVEEI EGVPPQISAT PGMPERKAVA RRQARVREML HTLPKKAQAA ILDDLEGTHK YAVHEIGQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc gatacagtga ctcataccac acaacgggcc ggtggcagcc acgagcgtcg acagaagggt ttcccatggg cgtcagcatc gaggtcaacg gactaacgaa gtccttcggg tcctcgagga tctgggaaga tgtcacgcta acgatccccg ccggggaggt cagcgtgctg ctgggcccat cgggtaccgg caaatcggtg tttctgaaat ctctgatcgg cctcctgcgg ccggagcgcg gctcgatcat catcgacggc accgacatca tcgaatgctc ggccaaggag ctttacgaga tccgcacatt gttcggcgtg ctgtttcagg acggtgccct gttcgggtcg atgaacctct acgacaacac cgcgttcccc ctgcgtgagc acaccaagaa aaaggaaagc gagatccgtg acatcgtcat ggagaagctg gccctagtcg gcctgggtgg ggacgagaag aagttccccg gcgagatctc cggcgggatg cgtaagcgtg ccggcctagc gcgtgccctg gtccttgacc cgcagatcat tctctgcgac gagcccgact cgggtctgga cccggttcgt accgcctacc tgagccagct gatcatggac atcaacgccc agatcgacgc caccatcctg atcgtgacgc acaacatcaa catcgcccgc accgtgccgg acaacatggg catgttgttc cgcaagcatt tggtgatgtt cgggccgcgg gaggtgctac tcaccagcga cgagccggtg gtgcggcagt tcctcaacgg ccggcgcatc ggcccgatcg gcatgtccga ggagaaggac gaggccacca tggccgaaga gcaggccctg ctcgatgccg gccaccacgc gggcggtgtc gaggaaatcg agggcgtgcc gccgcagatc agcgcgacac cgggcatgcc ggagcgcaaa gcggtcgccc ggcgtcaggc tcgggttcgc gagatgttgc acacgctgcc caaaaaggcc caggcggcga tcctcgacga tctcgagggc acgcacaagt acgcggtgca cgaaatcggc cagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MytuD.00656.a.B1.GE27587
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 40kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV RYSDSYHTTG RWQPRASTEG FPMGVSIEVN GLTKSFGSSR IWEDVTLTIP AGEVSVLLGP SGTGKSVFLK SLIGLLRPER GSIIIDGTDI IECSAKELYE IRTLFGVLFQ DGALFGSMNL YDNTAFPLRE HTKKKESEIR DIVMEKLALV GLGGDEKKFP GEISGGMRKR AGLARALVLD PQIILCDEPD SGLDPVRTAY LSQLIMDINA QIDATILIVT HNINIARTVP DNMGMLFRKH LVMFGPREVL LTSDEPVVRQ FLNGRRIGPI GMSEEKDEAT MAEEQALLDA GHHAGGVEEI EGVPPQISAT PGMPERKAVA RRQARVREML HTLPKKAQAA ILDDLEGTHK YAVHEIGQ
Validated NT Sequence
cttactggcc gatttcgtgc accgcgtact tgtgcgtgcc ctcgagatcg tcgaggatcg ccgcctgggc ctttttgggc agcgtgtgca acatctcgcg aacccgagcc tgacgccggg cgaccgcttt gcgctccggc atgcccggtg tcgcgctgat ctgcggcggc acgccctcga tttcctcgac accgcccgcg tggtggccgg catcgagcag ggcctgctct tcggccatgg tggcctcgtc cttctcctcg gacatgccga tcgggccgat gcgccggccg ttgaggaact gccgcaccac cggctcgtcg ctggtgagta gcacctcccg cggcccgaac atcaccaaat gcttgcggaa caacatgccc atgttgtccg gcacggtgcg ggcgatgttg atgttgtgcg tcacgatcag gatggtggcg tcgatctggg cgttgatgtc catgatcagc tggctcaggt aggcggtacg aaccgggtcc agacccgagt cgggctcgtc gcagagaatg atctgcgggt caaggaccag ggcacgcgct aggccggcac gcttacgcat cccgccggag atctcgccgg ggaacttctt ctcgtcccca cccaggccga ctagggccag cttctccatg acgatgtcac ggatctcgct ttcctttttc ttggtgtgct cacgcagggg gaacgcggtg ttgtcgtaga ggttcatcga cccgaacagg gcaccgtcct gaaacagcac gccgaacaat gtgcggatct cgtaaagctc cttggccgag cattcgatga tgtcggtgcc gtcgatgatg atcgagccgc gctccggccg caggaggccg atcagagatt tcagaaacac cgatttgccg gtacccgatg ggcccagcag cacgctgacc tccccggcgg ggatcgttag cgtgacatct tcccagatcc tcgaggaccc gaaggacttc gttagtccgt tgacctcgat gctgacgccc atgggaaacc cttctgtcga cgctcgtggc tgccaccggc ccgttgtgtg gtatgagtca ctgtatcgca ccatatggtg gtggtggtgg tgagccatgg tatnnctcct t
Expected Protein Sequence
MAHHHHHHMV RYSDSYHTTG RWQPRASTEG FPMGVSIEVN GLTKSFGSSR IWEDVTLTIP AGEVSVLLGP SGTGKSVFLK SLIGLLRPER GSIIIDGTDI IECSAKELYE IRTLFGVLFQ DGALFGSMNL YDNTAFPLRE HTKKKESEIR DIVMEKLALV GLGGDEKKFP GEISGGMRKR AGLARALVLD PQIILCDEPD SGLDPVRTAY LSQLIMDINA QIDATILIVT HNINIARTVP DNMGMLFRKH LVMFGPREVL LTSDEPVVRQ FLNGRRIGPI GMSEEKDEAT MAEEQALLDA GHHAGGVEEI EGVPPQISAT PGMPERKAVA RRQARVREML HTLPKKAQAA ILDDLEGTHK YAVHEIGQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc gatacagtga ctcataccac acaacgggcc ggtggcagcc acgagcgtcg acagaagggt ttcccatggg cgtcagcatc gaggtcaacg gactaacgaa gtccttcggg tcctcgagga tctgggaaga tgtcacgcta acgatccccg ccggggaggt cagcgtgctg ctgggcccat cgggtaccgg caaatcggtg tttctgaaat ctctgatcgg cctcctgcgg ccggagcgcg gctcgatcat catcgacggc accgacatca tcgaatgctc ggccaaggag ctttacgaga tccgcacatt gttcggcgtg ctgtttcagg acggtgccct gttcgggtcg atgaacctct acgacaacac cgcgttcccc ctgcgtgagc acaccaagaa aaaggaaagc gagatccgtg acatcgtcat ggagaagctg gccctagtcg gcctgggtgg ggacgagaag aagttccccg gcgagatctc cggcgggatg cgtaagcgtg ccggcctagc gcgtgccctg gtccttgacc cgcagatcat tctctgcgac gagcccgact cgggtctgga cccggttcgt accgcctacc tgagccagct gatcatggac atcaacgccc agatcgacgc caccatcctg atcgtgacgc acaacatcaa catcgcccgc accgtgccgg acaacatggg catgttgttc cgcaagcatt tggtgatgtt cgggccgcgg gaggtgctac tcaccagcga cgagccggtg gtgcggcagt tcctcaacgg ccggcgcatc ggcccgatcg gcatgtccga ggagaaggac gaggccacca tggccgaaga gcaggccctg ctcgatgccg gccaccacgc gggcggtgtc gaggaaatcg agggcgtgcc gccgcagatc agcgcgacac cgggcatgcc ggagcgcaaa gcggtcgccc ggcgtcaggc tcgggttcgc gagatgttgc acacgctgcc caaaaaggcc caggcggcga tcctcgacga tctcgagggc acgcacaagt acgcggtgca cgaaatcggc cagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat