MymaA.00656.a

Ribonucleotide-transport ATP-binding protein ABC transporter, Mkl

CENTER ID: MymaA.00656.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00656.a.A1.GE30056 Full length( MymaA.00656.a ) 1 328
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1061
RefSeq: YP_001849305.1
UniProt: B2HSJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGVAIEVNGL TKSFGSSRIW EDVTLTIPAG EVSVLLGPSG TGKSVFLKSL IGLLRPERGS ILIDGTDIIE CSAKELYEIR TLFGVLFQDG ALFGSMNLYD NTAFPLREHT KKKESEIRDI VMEKLAMVGL TGDEKKFPGE ISGGMRKRAG LARALVLDPQ IILCDEPDSG LDPVRTAYLS QLIMDINAQI DATILIVTHN INIARTVPDN MGMLFRKHLV MFGPREVLLT SDEPVVRQFL NGRRIGPIGM SEEKDEATMA EEQAMIDAGQ HAGGTEDIEG VPPQIQATPG MPERKAVARR QARVREMLHT LPKDAQAAIL EDLEGTHR
NT Sequence
ATGGGCGTCG CTATCGAGGT CAACGGACTG ACAAAGTCCT TCGGATCCTC GAGGATTTGG GAAGACGTCA CGCTGACGAT CCCGGCCGGG GAGGTCAGCG TGTTGCTTGG TCCCTCCGGT ACTGGCAAGT CGGTGTTCCT GAAGTCCCTC ATCGGTCTGC TGCGCCCAGA GCGCGGCTCG ATCCTCATCG ATGGCACCGA CATCATCGAA TGTTCGGCCA AGGAGCTCTA CGAGATCCGG ACGTTGTTCG GTGTGCTGTT CCAGGACGGT GCGCTGTTCG GGTCGATGAA CCTCTATGAC AACACCGCCT TCCCGCTGCG TGAGCACACC AAGAAGAAGG AAAGCGAAAT CCGTGACATC GTCATGGAGA AGCTGGCCAT GGTCGGTCTC ACCGGTGATG AGAAGAAGTT CCCCGGTGAG ATCTCCGGTG GTATGCGCAA GCGCGCCGGC CTGGCCAGGG CGCTGGTTCT CGACCCGCAG ATCATTCTCT GCGACGAGCC CGACTCGGGT CTGGACCCCG TCCGTACCGC CTATCTGAGC CAGTTGATCA TGGACATCAA CGCCCAGATC GATGCCACCA TCCTGATCGT GACGCACAAC ATCAACATCG CGCGCACCGT GCCGGACAAC ATGGGCATGT TGTTCCGTAA GCACCTGGTC ATGTTCGGCC CCCGCGAGGT GCTGCTCACC AGCGATGAGC CCGTGGTTCG GCAGTTCCTC AACGGCCGCC GCATCGGCCC GATCGGCATG TCCGAGGAAA AGGACGAGGC CACCATGGCC GAAGAGCAGG CCATGATCGA CGCCGGACAG CACGCCGGCG GCACCGAGGA CATCGAGGGT GTGCCACCGC AGATCCAAGC GACTCCGGGC ATGCCGGAAC GTAAGGCCGT CGCCCGCCGC CAGGCACGGG TTCGCGAGAT GCTGCACACG CTGCCCAAGG ACGCCCAGGC GGCAATCCTC GAGGACCTCG AGGGCACCCA CCGC
Details for MymaA.00656.a.A1.GE30056
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 106
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGVAIEVNG LTKSFGSSRI WEDVTLTIPA GEVSVLLGPS GTGKSVFLKS LIGLLRPERG SILIDGTDII ECSAKELYEI RTLFGVLFQD GALFGSMNLY DNTAFPLREH TKKKESEIRD IVMEKLAMVG LTGDEKKFPG EISGGMRKRA GLARALVLDP QIILCDEPDS GLDPVRTAYL SQLIMDINAQ IDATILIVTH NINIARTVPD NMGMLFRKHL VMFGPREVLL TSDEPVVRQF LNGRRIGPIG MSXEKDXATM AEEQAMIDAG QHAGGTEDIE GVPPXIXATP XXXERKXSXX RQARXRXMLX XXPAQXAILX XSXXXLXXXX XXXXXXXXXX XXXXXXLXXX X
Validated NT Sequence
ttgtttanct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgggcgtc gctatcgagg tcaacggact gacaaagtcc ttcggatcct cgaggatttg ggaagacgtc acgctgacga tcccggccgg ggaggtcagc gtgttgcttg gtccctccgg tactggcaag tcggtgttcc tgaagtccct catcggtctg ctgcgcccag agcgcggctc gatcctcatc gatggcaccg acatcatcga atgttcggcc aaggagctct acgagatccg gacgttgttc ggtgtgctgt tccaggacgg tgcgctgttc gggtcgatga acctctatga caacaccgcc ttcccgctgc gtgagcacac caagaagaag gaaagcgaaa tccgtgacat cgtcatggag aagctggcca tggtcggtct caccggtgat gagaagaagt tccccggtga gatctccggt ggtatgcgca agcgcgccgg cctggccagg gcgctggttc tcgacccgca gatcattctc tgcgacgagc ccgactcggg tctggacccc gtccgtaccg cctatctgag ccagttgatc atggacatca acgcccagat cgatgccacc atcctgatcg tgacgcacaa catcaacatc gcgcgcaccg tgccggacaa catgggcatg ttgttccgta agcacctggt catgttcggc ccccgcgagg tgctgctcac cagcgatgag cccgtggttc ggcagttcct caacggccgc cgcatcggcc cgatcggcat gtccgangaa aaggacnagg ccaccatggc cgaagagcag gccatgatcg acgccggaca gcacgccggc ggcaccgagg acatcgaggg tgtgccaccg canatccnag cgactccngn nntgcnggaa cgtaagnnnt cgncccnccg ccaggcacgg gnncgcnnga tgctnnacnn ncnncccgcc cagnnngcaa tcctcnngnn ntcnangnnn cnnctantaa nnnncangan nannnngnnn nnngnnnnnn nngnnnnnnn ctnnnnannc nnaaanctnn nnnngnngnc nc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGVAIEVNG LTKSFGSSRI WEDVTLTIPA GEVSVLLGPS GTGKSVFLKS LIGLLRPERG SILIDGTDII ECSAKELYEI RTLFGVLFQD GALFGSMNLY DNTAFPLREH TKKKESEIRD IVMEKLAMVG LTGDEKKFPG EISGGMRKRA GLARALVLDP QIILCDEPDS GLDPVRTAYL SQLIMDINAQ IDATILIVTH NINIARTVPD NMGMLFRKHL VMFGPREVLL TSDEPVVRQF LNGRRIGPIG MSEEKDEATM AEEQAMIDAG QHAGGTEDIE GVPPQIQATP GMPERKAVAR RQARVREMLH TLPKDAQAAI LEDLEGTH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgtcgct atcgaggtca acggactgac aaagtccttc ggatcctcga ggatttggga agacgtcacg ctgacgatcc cggccgggga ggtcagcgtg ttgcttggtc cctccggtac tggcaagtcg gtgttcctga agtccctcat cggtctgctg cgcccagagc gcggctcgat cctcatcgat ggcaccgaca tcatcgaatg ttcggccaag gagctctacg agatccggac gttgttcggt gtgctgttcc aggacggtgc gctgttcggg tcgatgaacc tctatgacaa caccgccttc ccgctgcgtg agcacaccaa gaagaaggaa agcgaaatcc gtgacatcgt catggagaag ctggccatgg tcggtctcac cggtgatgag aagaagttcc ccggtgagat ctccggtggt atgcgcaagc gcgccggcct ggccagggcg ctggttctcg acccgcagat cattctctgc gacgagcccg actcgggtct ggaccccgtc cgtaccgcct atctgagcca gttgatcatg gacatcaacg cccagatcga tgccaccatc ctgatcgtga cgcacaacat caacatcgcg cgcaccgtgc cggacaacat gggcatgttg ttccgtaagc acctggtcat gttcggcccc cgcgaggtgc tgctcaccag cgatgagccc gtggttcggc agttcctcaa cggccgccgc atcggcccga tcggcatgtc cgaggaaaag gacgaggcca ccatggccga agagcaggcc atgatcgacg ccggacagca cgccggcggc accgaggaca tcgagggtgt gccaccgcag atccaagcga ctccgggcat gccggaacgt aaggccgtcg cccgccgcca ggcacgggtt cgcgagatgc tgcacacgct gcccaaggac gcccaggcgg caatcctcga ggacctcgag ggcacccaca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga 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