MyleA.00656.a

ABC transporter ATP-binding protein

CENTER ID: MyleA.00656.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00656.a.A1.GE29227 Full length( MyleA.00656.a ) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.3603
RefSeq: YP_002503904.1
UniProt: A0A0H3MRJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPMGVAIEVK GLTKSFGSSR IWEDVTLDIP AGEVSVLLGP SGTGKSVFLK SLIGLLRPER GSILIDGTDI IECSAKELYE IRTLFGVLFQ DGALFGSMNL YDNTAFPLRE HTKKKESEIR DIVMEKLQLV GLGGDEKKFP GEISGGMRKR AGLARALVLD PQIILCDEPD SGLDPVRTAY LSQLIMDINA QIDATILIVT HNVNIARTVP DNMGMLFRKH LVMFGPREVL LTSDEPVVRQ FLNGRRIGPI GMSEEKDEST MAEEAALLEA GHYAGGAEEV EGVPPQITVT PGMPKRKAVA RRQARVRAML PTLPKGAQAA ILDDLEGAHN YQAHEFGD
NT Sequence
ATGCCCATGG GCGTTGCGAT CGAAGTTAAG GGACTAACCA AGTCTTTTGG ATCTTCGAGG ATTTGGGAAG ACGTCACGCT TGACATTCCC GCAGGAGAGG TCAGTGTGTT ACTCGGGCCG TCGGGTACCG GCAAATCAGT GTTCTTGAAG TCCCTCATCG GTCTGTTGCG GCCGGAACGT GGCTCGATCC TAATTGATGG TACGGACATC ATCGAATGTT CAGCCAAGGA ACTCTACGAG ATCCGCACAC TGTTCGGCGT GCTATTCCAG GACGGCGCGC TGTTCGGCTC GATGAACCTC TACGACAACA CTGCGTTCCC ATTGCGTGAG CACACCAAGA AAAAGGAAAG CGAGATCCGT GACATCGTCA TGGAGAAGCT GCAGCTGGTC GGCCTTGGTG GGGACGAGAA GAAGTTCCCC GGCGAGATCT CCGGCGGTAT GCGCAAGCGT GCCGGTCTAG CGCGAGCCTT GGTTCTTGAC CCGCAGATCA TTCTGTGCGA CGAGCCCGAC TCGGGTCTGG ACCCGGTCCG TACCGCTTAC CTGAGTCAGC TGATCATGGA CATCAATGCC CAGATTGATG CGACCATCCT AATCGTGACG CACAACGTCA ACATCGCCCG CACTGTGCCC GACAACATGG GCATGTTGTT CCGCAAGCAT CTGGTAATGT TCGGACCCCG TGAGGTGCTG CTAACCAGCG ACGAGCCGGT TGTACGGCAA TTCCTCAACG GCCGTCGTAT CGGCCCGATT GGGATGTCCG AGGAGAAGGA CGAGTCCACC ATGGCTGAAG AGGCAGCTTT GCTCGAAGCA GGTCACTACG CGGGTGGCGC CGAGGAAGTC GAGGGTGTGC CGCCGCAGAT CACCGTGACA CCGGGGATGC CGAAGCGCAA AGCGGTCGCA CGCCGCCAGG CCCGGGTGCG GGCGATGTTG CCTACACTGC CCAAGGGTGC CCAGGCGGCG ATCCTCGATG ATCTCGAGGG CGCCCACAAT TACCAAGCGC ACGAATTCGG CGAC
Details for MyleA.00656.a.A1.GE29227
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 39kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MPMGVAIEVK GLTKSFGSSR IWEDVTLDIP AGEVSVLLGP SGTGKSVFLK SLIGLLRPER GSILIDGTDI IECSAKELYE IRTLFGVLFQ DGALFGSMNL YDNTAFPLRE HTKKKESEIR DIVMEKLQLV GLGGDEKKFP GEISGGMRKR AGLARALVLD PQIILCDEPD SGLDPVRTAY LSQLIMDINA QIDATILIVT HNVNIARTVP DNMGMLFRKH LVMFGPREVL LTSDEPVVRQ FLNGRRIGPI GMSEEKDEST MAEEAALLEA GHYAGGAEEV EGVPPQITVT PGMPKRKAVA RRQARVRAML PTLPKGAQAA ILDDLEGAHN YQAHEFG
Validated NT Sequence
cctggaagct cagacccagg gtcctggttc gatgcccatg ggcgttgcga tcgaagttaa gggactaacc aagtcttttg gatcttcgag gatttgggaa gacgtcacgc ttgacattcc cgcaggagag gtcagtgtgt tactcgggcc gtcgggtacc ggcaaatcag tgttcttgaa gtccctcatc ggtctgttgc ggccggaacg tggctcgatc ctaattgatg gtacggacat catcgaatgt tcagccaagg aactctacga gatccgcaca ctgttcggcg tgctattcca ggacggcgcg ctgttcggct cgatgaacct ctacgacaac actgcgttcc cattgcgtga gcacaccaag aaaaaggaaa gcgagatccg tgacatcgtc atggagaagc tgcagctggt cggccttggt ggggacgaga agaagttccc cggcgagatc tccggcggta tgcgcaagcg tgccggtcta gcgcgagcct tggttcttga cccgcagatc attctgtgcg acgagcccga ctcgggtctg gacccggtcc gtaccgctta cctgagtcag ctgatcatgg acatcaatgc ccagattgat gcgaccatcc taatcgtgac gcacaacgtc aacatcgccc gcactgtgcc cgacaacatg ggcatgttgt tccgcaagca tctggtaatg ttcggacccc gtgaggtgct gctaaccagc gacgagccgg ttgtacggca attcctcaac ggccgtcgta tcggcccgat tgggatgtcc gaggagaagg acgagtccac catggctgaa gaggcagctt tgctcgaagc aggtcactac gcgggtggcg ccgaggaagt cgagggtgtg ccgccgcaga tcaccgtgac accggggatg ccgaagcgca aagcggtcgc acgccgccag gcccgggtgc gggcgatgtt gcctacactg cccaagggtg cccaggcggc gatcctcgat gatctcgagg gcgcccacaa ttaccaagcg cacgaattcg gcgac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPMGVAIEV KGLTKSFGSS RIWEDVTLDI PAGEVSVLLG PSGTGKSVFL KSLIGLLRPE RGSILIDGTD IIECSAKELY EIRTLFGVLF QDGALFGSMN LYDNTAFPLR EHTKKKESEI RDIVMEKLQL VGLGGDEKKF PGEISGGMRK RAGLARALVL DPQIILCDEP DSGLDPVRTA YLSQLIMDIN AQIDATILIV THNVNIARTV PDNMGMLFRK HLVMFGPREV LLTSDEPVVR QFLNGRRIGP IGMSEEKDES TMAEEAALLE AGHYAGGAEE VEGVPPQITV TPGMPKRKAV ARRQARVRAM LPTLPKGAQA AILDDLEGAH NYQAHEFGD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccatgggc gttgcgatcg aagttaaggg actaaccaag tcttttggat cttcgaggat ttgggaagac gtcacgcttg acattcccgc aggagaggtc agtgtgttac tcgggccgtc gggtaccggc aaatcagtgt tcttgaagtc cctcatcggt ctgttgcggc cggaacgtgg ctcgatccta attgatggta cggacatcat cgaatgttca gccaaggaac tctacgagat ccgcacactg ttcggcgtgc tattccagga cggcgcgctg ttcggctcga tgaacctcta cgacaacact gcgttcccat tgcgtgagca caccaagaaa aaggaaagcg agatccgtga catcgtcatg gagaagctgc agctggtcgg ccttggtggg gacgagaaga agttccccgg cgagatctcc ggcggtatgc gcaagcgtgc cggtctagcg cgagccttgg ttcttgaccc gcagatcatt ctgtgcgacg agcccgactc gggtctggac ccggtccgta ccgcttacct gagtcagctg atcatggaca tcaatgccca gattgatgcg accatcctaa tcgtgacgca caacgtcaac atcgcccgca ctgtgcccga caacatgggc atgttgttcc gcaagcatct ggtaatgttc ggaccccgtg aggtgctgct aaccagcgac gagccggttg tacggcaatt cctcaacggc cgtcgtatcg gcccgattgg gatgtccgag gagaaggacg agtccaccat ggctgaagag gcagctttgc tcgaagcagg tcactacgcg ggtggcgccg aggaagtcga gggtgtgccg ccgcagatca ccgtgacacc ggggatgccg aagcgcaaag cggtcgcacg ccgccaggcc cgggtgcggg cgatgttgcc tacactgccc aagggtgccc aggcggcgat cctcgatgat ctcgagggcg cccacaatta ccaagcgcac gaattcggcg acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa