MytuD.00654.a

acetyl-CoA acetyltransferase

CENTER ID: MytuD.00654.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00654.a.A1.GE30667 Full length( MytuD.00654.a ) 1 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3960
RefSeq: NP_218063.1
TubercuList: Rv3546
MTB Network Portal: Rv3546
UniProt: I6XHI4

Orthologues for Rv3546

Species % Identity % Coverage Clones Proteins Structures
marinum 90 100 1 0 0
ulcerans 90 100 1 0 0
avium 86 100 0 0 0
paratuberculosis 86 100 0 0 0
smegmatis 83 100 0 1 0
abscessus 76 100 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGYPVIVEAT RSPIGKRNGW LSGLHATELL GAVQKAVVDK AGIQSGLHAG DVEQVIGGCV TQFGEQSNNI SRVAWLTAGL PEHVGATTVD CQCGSGQQAN HLIAGLIAAG AIDVGIACGI EAMSRVGLGA NAGPDRSLIR AQSWDIDLPN QFEAAERIAK RRGITREDVD VFGLESQRRA QRAWAEGRFD REISPIQAPV LDEQNQPTGE RRLVFRDQGL RETTMAGLGE LKPVLEGGIH TAGTSSQISD GAAAVLWMDE AVARAHGLTP RARIVAQALV GAEPYYHLDG PVQSTAKVLE KAGMKIGDID IVEINEAFAS VVLSWARVHE PDMDRVNVNG GAIALGHPVG CTGSRLITTA LHELERTDQS LALITMCAGG ALSTGTIIER I
NT Sequence
ATGGGTTACC CGGTCATCGT TGAAGCCACC CGCAGCCCCA TCGGCAAACG CAACGGATGG CTGTCGGGGC TGCATGCCAC CGAGTTGTTG GGCGCGGTGC AAAAGGCGGT GGTCGACAAG GCCGGCATCC AGTCCGGCCT TCACGCCGGT GACGTCGAAC AGGTCATCGG CGGTTGCGTG ACCCAGTTCG GGGAGCAATC CAACAACATC AGCCGGGTGG CCTGGCTGAC GGCCGGTTTG CCCGAACACG TCGGCGCCAC CACCGTCGAC TGCCAGTGCG GCAGCGGCCA GCAGGCCAAC CATCTGATTG CCGGGTTGAT CGCGGCCGGT GCCATCGATG TCGGCATCGC CTGCGGCATC GAGGCGATGA GCCGGGTCGG GCTGGGCGCC AACGCCGGGC CGGACCGCTC GCTGATCCGC GCGCAGTCAT GGGATATCGA CCTGCCGAAC CAGTTCGAGG CCGCCGAGCG GATCGCCAAG CGGCGCGGCA TCACCCGCGA GGACGTGGAT GTCTTCGGGC TCGAGTCGCA GCGACGCGCG CAGCGGGCCT GGGCGGAGGG CCGCTTTGAC CGCGAGATCT CGCCGATCCA GGCGCCGGTG CTCGACGAGC AGAATCAGCC CACCGGCGAG CGGCGCCTGG TCTTTCGCGA CCAGGGCCTG CGCGAGACCA CGATGGCGGG GCTAGGCGAG CTGAAACCGG TGCTCGAGGG CGGCATCCAC ACCGCGGGCA CGTCGTCGCA GATCTCCGAC GGCGCGGCAG CCGTGTTGTG GATGGACGAA GCCGTGGCAC GTGCGCACGG CCTGACCCCG CGGGCCCGGA TCGTCGCCCA GGCACTCGTC GGCGCCGAGC CCTACTACCA CCTGGACGGC CCGGTGCAGT CCACCGCGAA GGTGCTGGAG AAGGCCGGCA TGAAGATCGG CGACATCGAC ATCGTCGAGA TCAACGAGGC GTTCGCGTCC GTGGTGCTGT CCTGGGCGCG GGTGCACGAG CCCGACATGG ACCGGGTCAA CGTCAACGGC GGGGCGATCG CGCTGGGGCA TCCGGTGGGC TGCACCGGCA GCCGGCTGAT CACCACCGCC CTGCACGAGC TCGAGCGCAC CGACCAGAGC CTCGCGCTGA TCACCATGTG CGCCGGCGGG GCCCTGTCCA CCGGCACCAT CATCGAGCGG ATT
Details for MytuD.00654.a.A1.GE30667
HARVESTED ON: 10/27/2010
SEQUENCED ON: 11/3/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 94
Validated AA Sequence
MGYPVIVEAT RSPIGKRNGW LSGLHATELL GAVQKAVVDK AGIQSGLHAG DVEQVIGGCV XXFGEQSNNI SRVAWLTAGL PEHVGATTVD CQCGSGQQAN HLIAGLIAAG AIDVGIACGI EAMSRVGLGA NAGPDRSLIR AQSWDIDLPN QFEAAERIAK RRXITREDVD VFXXESXRRA XRAWXXGRFX XXISXIXAPX LDEQNXPTGE RRLVFRDQGL RETTMAGLGE LKPVLEGGIH TAGTSSQXSD GAAAVXXMDE AVARAHGLTP RARIVAQALV GAEPYYHLDG PVQSTAKVLE KAGMKIGDID IVEINEAFAS VVLSWARVHE PDMDRVNVNG GAIALGHPVG CTGSRLITTA LHELERTDQS LALIXMCAGG ALSTGTIIER I
Validated NT Sequence
ggtaccctgg aagctcagac ccagggtcct ggttcgatgg gttacccggt catcgttgaa gccacccgca gccccatcgg caaacgcaac ggatggctgt cggggctgca tgccaccgag ttgttgggcg cggtgcaaaa ggcggtggtc gacaaggccg gcatccagtc cggccttcac gccggtgacg tcgaacaggt catcggcggt tgcgtgncnn nnttcgggga gcaatccaac aacatcagcc gggtggcctg gctgacggcc ggtttgcccg aacacgtcgg cgccaccacc gtcgactgcc agtgcggcag cggccagcag gccaaccatc tgattgccgg gttgatcgcg gccggtgcca tcgatgtcgg catcgcctgc ggcatcgagg cgatgagccg ggtcgggctg ggcgccaacg ccgggccgga ccgctcgctg atccgcgcgc agtcatggga tatcgacctg ccgaaccagt tcgaggccgc cgagcggatc gccaagcggc gcgncatcac ccgcgaggac gtggatgtct tcnnnntnga gtcgcancga cgcgcgnanc gggcctggnc gganggccgc tttgannncn agatctcgnc natccnngcg ccngngctcg acgagcagaa tcancccacc ggcgagcggc gcctggtctt tcgcgaccag ggcctgcgcg agaccacgat ggcggggcta ggcgagctga aaccggtgct cgagggcggc atccacaccg cgggcacgtc gtcgcagatn tccgacggcg cggcagccgt gtnnnggatg gacgaagccg tggcacgtgc gcacggcctg accccgcggg cccggatcgt cgcccaggca ctcgtcggcg ccgagcccta ctaccacctg gacggcccgg tgcagtccac cgcgaaggtg ctggagaagg ccggcatgaa gatcggcgac atcgacatcg tcgagatcaa cgaggcgttc gcgtccgtgg tgctgtcctg ggcgcgggtg cacgagcccg acatggaccg ggtcaacgtc aacggcgggg cgatcgcgct ggggcatccg gtgggctgca ccggcagccg gctgatcacc accgccctgc acgagctcga gcgcaccgac cagagcctcg cgctgatcnc catgtgcgcc ggcggggccc tgtccaccgg caccatcatc gagcggattt aatna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGYPVIVEA TRSPIGKRNG WLSGLHATEL LGAVQKAVVD KAGIQSGLHA GDVEQVIGGC VTQFGEQSNN ISRVAWLTAG LPEHVGATTV DCQCGSGQQA NHLIAGLIAA GAIDVGIACG IEAMSRVGLG ANAGPDRSLI RAQSWDIDLP NQFEAAERIA KRRGITREDV DVFGLESQRR AQRAWAEGRF DREISPIQAP VLDEQNQPTG ERRLVFRDQG LRETTMAGLG ELKPVLEGGI HTAGTSSQIS DGAAAVLWMD EAVARAHGLT PRARIVAQAL VGAEPYYHLD GPVQSTAKVL EKAGMKIGDI DIVEINEAFA SVVLSWARVH EPDMDRVNVN GGAIALGHPV GCTGSRLITT ALHELERTDQ SLALITMCAG GALSTGTIIE RI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggttacccg gtcatcgttg aagccacccg cagccccatc ggcaaacgca acggatggct gtcggggctg catgccaccg agttgttggg cgcggtgcaa aaggcggtgg tcgacaaggc cggcatccag tccggccttc acgccggtga cgtcgaacag gtcatcggcg gttgcgtgac ccagttcggg gagcaatcca acaacatcag ccgggtggcc tggctgacgg ccggtttgcc cgaacacgtc ggcgccacca ccgtcgactg ccagtgcggc agcggccagc aggccaacca tctgattgcc gggttgatcg cggccggtgc catcgatgtc ggcatcgcct gcggcatcga ggcgatgagc cgggtcgggc tgggcgccaa cgccgggccg gaccgctcgc tgatccgcgc gcagtcatgg gatatcgacc tgccgaacca gttcgaggcc gccgagcgga tcgccaagcg gcgcggcatc acccgcgagg acgtggatgt cttcgggctc gagtcgcagc gacgcgcgca gcgggcctgg gcggagggcc gctttgaccg cgagatctcg ccgatccagg cgccggtgct cgacgagcag aatcagccca ccggcgagcg gcgcctggtc tttcgcgacc agggcctgcg cgagaccacg atggcggggc taggcgagct gaaaccggtg ctcgagggcg gcatccacac cgcgggcacg tcgtcgcaga tctccgacgg cgcggcagcc gtgttgtgga tggacgaagc cgtggcacgt gcgcacggcc tgaccccgcg ggcccggatc gtcgcccagg cactcgtcgg cgccgagccc tactaccacc tggacggccc ggtgcagtcc accgcgaagg tgctggagaa ggccggcatg aagatcggcg acatcgacat cgtcgagatc aacgaggcgt tcgcgtccgt ggtgctgtcc tgggcgcggg tgcacgagcc cgacatggac cgggtcaacg tcaacggcgg ggcgatcgcg ctggggcatc cggtgggctg caccggcagc cggctgatca ccaccgccct gcacgagctc gagcgcaccg accagagcct cgcgctgatc accatgtgcg ccggcggggc cctgtccacc ggcaccatca tcgagcggat taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa