MysmA.00654.c

Acetyl-CoA acetyltransferase

CENTER ID: MysmA.00654.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00654.c.A1.PS00605 Full length( MysmA.00654.c ) 1 387

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5832
RefSeq: YP_890222.1
UniProt: A0R4Y4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGNPVIVEAT RSPIGKRNGW LSGLHATELL GAVQKALVEK AGIDPGSVEQ IIGGCVTQYG EQSNNITRVG WLTAGLPEHV GATTIDCQCG SAQQANHLVA GLIATGAIDI GIACGIEAMS RVGLGANAGP DRSLIRASSW DIDMPNQFEA AERIAQRRGI TRADVDALGL ASQRKAKQAW DEGRFDREIS PISAPVLDEN KQPTSEWAPV TRDQGLRDTT PEALASLKPV LDGGIHTAGT SSQISDGAAA VLWMDEDKAK ALGLKPRARI VAQANVGAET YYHLDGPVQS TARVLEKAGM KLGDIDLVEI NEAFASVVLS WAQVHNADMD KVNVNGGAIA LGHPVGSTGA RLITTALHEL ERTGKGTALI TMCAGGALST GTIIERI
NT Sequence
ATGGGTAATC CTGTCATCGT CGAAGCCACC CGTAGCCCGA TCGGAAAACG CAACGGGTGG TTGTCCGGTC TGCACGCCAC GGAACTGCTG GGTGCGGTGC AGAAGGCGCT GGTGGAGAAG GCTGGCATCG ACCCCGGATC CGTCGAGCAG ATCATCGGTG GCTGCGTCAC CCAGTACGGC GAGCAGTCCA ACAACATCAC CCGCGTGGGG TGGCTGACCG CGGGATTGCC CGAGCACGTC GGCGCCACCA CCATCGACTG CCAGTGCGGC AGCGCGCAGC AGGCCAACCA TCTGGTGGCC GGCCTGATCG CGACGGGTGC GATCGACATC GGCATCGCGT GCGGTATCGA GGCCATGAGC CGCGTGGGCC TGGGCGCCAA CGCGGGCCCG GACCGTTCGC TGATCCGCGC GTCGTCGTGG GACATCGACA TGCCCAACCA GTTCGAGGCG GCCGAACGGA TCGCCCAGCG CCGCGGCATC ACGCGTGCCG ACGTCGACGC CCTCGGCCTG GCGTCGCAGC GCAAGGCCAA GCAGGCCTGG GACGAAGGCC GGTTCGACCG CGAGATCTCC CCCATCTCGG CCCCGGTGCT CGACGAGAAC AAGCAGCCCA CGTCGGAATG GGCTCCCGTC ACGCGCGACC AGGGTCTGCG TGACACCACA CCCGAAGCGC TGGCGTCGCT CAAGCCCGTG CTGGACGGCG GCATCCACAC CGCGGGAACG TCGTCGCAGA TCTCCGACGG CGCGGCCGCG GTGCTGTGGA TGGACGAGGA CAAGGCCAAG GCGCTCGGCC TCAAGCCGCG GGCGCGCATC GTCGCGCAGG CCAACGTCGG CGCCGAGACC TACTACCACC TCGACGGCCC GGTGCAGTCC ACCGCGCGGG TGCTGGAGAA GGCGGGCATG AAGCTCGGCG ACATCGACCT CGTCGAGATC AACGAGGCCT TCGCGTCGGT CGTGCTGTCG TGGGCACAGG TGCACAATGC CGACATGGAC AAGGTGAACG TCAACGGCGG CGCGATCGCG CTGGGCCACC CGGTCGGATC GACCGGCGCC CGCCTGATCA CCACGGCACT GCACGAACTG GAGCGCACCG GCAAGGGCAC CGCGCTCATC ACGATGTGCG CCGGCGGGGC GCTGTCCACG GGCACCATCA TCGAGCGCAT C
Details for MysmA.00654.c.A1.PS00605
PURIFICATION DATe: 5/14/2010
CONCENTRATION: 32.68mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 82
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGNPVIVEA TRSPIGKRNG WLSGLHATEL LGAVQKALVE KAGIDPGSVE QIIGGCVTQY GEQSNNITRV GWLTAGLPEH VGATTIDCQC GSAQQANHLV AGLIATGAID IGIACGIEAM SRVGLGANAG PDRSLIRASS WDIDMPNQFE AAERIAQRRG ITRADVDALG LASQRKAKQA WDEGRFDREI SPISAPVLDE NKQPTSEWAP VTRDQGLRDT TPEALASLKP VLDGGIHTAG TSSQISDGAA AVLWMDEDKA KALGLKPRAR IVAQANVGAX TYYHLXGPXQ STARVLXXXX XXXXXXXXXX XXVXXXXGXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
attttgttta nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgggt aatcctgtca tcgtcgaagc cacccgtagc ccgatcggaa aacgcaacgg gtggttgtcc ggtctgcacg ccacggaact gctgggtgcg gtgcagaagg cgctggtgga gaaggctggc atcgaccccg gatccgtcga gcagatcatc ggtggctgcg tcacccagta cggcgagcag tccaacaaca tcacccgcgt ggggtggctg accgcgggat tgcccgagca cgtcggcgcc accaccatcg actgccagtg cggcagcgcg cagcaggcca accatctggt ggccggcctg atcgcgacgg gtgcgatcga catcggcatc gcgtgcggta tcgaggccat gagccgcgtg ggcctgggcg ccaacgcggg cccggaccgt tcgctgatcc gcgcgtcgtc gtgggacatc gacatgccca accagttcga ggcggccgaa cggatcgccc agcgccgcgg catcacgcgt gccgacgtcg acgccctcgg cctggcgtcg cagcgcaagg ccaagcaggc ctgggacgaa ggccggttcg accgcgagat ctcccccatc tcggccccgg tgctcgacga gaacaagcag cccacgtcgg aatgggctcc cgtcacgcgc gaccagggtc tgcgtgacac cacacccgaa gcgctggcgt cgctcaagcc cgtgctggac ggcggcatcc acaccgcggg aacgtcgtcg cagatctccg acggcgcggc cgcggtgctg tggatggacg aggacaaggc caaggcgctc ggcctcaagc cgcgggcgcg catcgtcgcg caggccaacg tcggcgccga nacctactac cacctcgang gcccngngca gtccaccgcg cgggtgctng annggnatnn nntcngcnan nncnncncnn cnanatnann nnnnnnngtn cnnnnnntcn nnggnnnnnn nnnnnatncn nnnnnnnnan gnnnangtnn nnnnnnnnnt nnnnnnnnnn nccnnnngnn nnnnnnnnnn nnctnnnnnn nnnnnnnnnn nnngnnnnnn cnnnnnnnnn gnnnnnnnnn nnnnng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGNPVIVEA TRSPIGKRNG WLSGLHATEL LGAVQKALVE KAGIDPGSVE QIIGGCVTQY GEQSNNITRV GWLTAGLPEH VGATTIDCQC GSAQQANHLV AGLIATGAID IGIACGIEAM SRVGLGANAG PDRSLIRASS WDIDMPNQFE AAERIAQRRG ITRADVDALG LASQRKAKQA WDEGRFDREI SPISAPVLDE NKQPTSEWAP VTRDQGLRDT TPEALASLKP VLDGGIHTAG TSSQISDGAA AVLWMDEDKA KALGLKPRAR IVAQANVGAE TYYHLDGPVQ STARVLEKAG MKLGDIDLVE INEAFASVVL SWAQVHNADM DKVNVNGGAI ALGHPVGSTG ARLITTALHE LERTGKGTAL ITMCAGGALS TGTIIERI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTAATCCT GTCATCGTCG AAGCCACCCG TAGCCCGATC GGAAAACGCA ACGGGTGGTT GTCCGGTCTG CACGCCACGG AACTGCTGGG TGCGGTGCAG AAGGCGCTGG TGGAGAAGGC TGGCATCGAC CCCGGATCCG TCGAGCAGAT CATCGGTGGC TGCGTCACCC AGTACGGCGA GCAGTCCAAC AACATCACCC GCGTGGGGTG GCTGACCGCG GGATTGCCCG AGCACGTCGG CGCCACCACC ATCGACTGCC AGTGCGGCAG CGCGCAGCAG GCCAACCATC TGGTGGCCGG CCTGATCGCG ACGGGTGCGA TCGACATCGG CATCGCGTGC GGTATCGAGG CCATGAGCCG CGTGGGCCTG GGCGCCAACG CGGGCCCGGA CCGTTCGCTG ATCCGCGCGT CGTCGTGGGA CATCGACATG CCCAACCAGT TCGAGGCGGC CGAACGGATC GCCCAGCGCC GCGGCATCAC GCGTGCCGAC GTCGACGCCC TCGGCCTGGC GTCGCAGCGC AAGGCCAAGC AGGCCTGGGA CGAAGGCCGG TTCGACCGCG AGATCTCCCC CATCTCGGCC CCGGTGCTCG ACGAGAACAA GCAGCCCACG TCGGAATGGG CTCCCGTCAC GCGCGACCAG GGTCTGCGTG ACACCACACC CGAAGCGCTG GCGTCGCTCA AGCCCGTGCT GGACGGCGGC ATCCACACCG CGGGAACGTC GTCGCAGATC TCCGACGGCG CGGCCGCGGT GCTGTGGATG GACGAGGACA AGGCCAAGGC GCTCGGCCTC AAGCCGCGGG CGCGCATCGT CGCGCAGGCC AACGTCGGCG CCGAGACCTA CTACCACCTC GACGGCCCGG TGCAGTCCAC CGCGCGGGTG CTGGAGAAGG CGGGCATGAA GCTCGGCGAC ATCGACCTCG TCGAGATCAA CGAGGCCTTC GCGTCGGTCG TGCTGTCGTG GGCACAGGTG CACAATGCCG ACATGGACAA GGTGAACGTC AACGGCGGCG CGATCGCGCT GGGCCACCCG GTCGGATCGA CCGGCGCCCG CCTGATCACC ACGGCACTGC ACGAACTGGA GCGCACCGGC AAGGGCACCG CGCTCATCAC GATGTGCGCC GGCGGGGCGC TGTCCACGGG CACCATCATC GAGCGCATCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A