MymaA.00654.b

Acetyl-CoA acetyltransferase FadA5

CENTER ID: MymaA.00654.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00654.b.A1.GE30054 Full length( MymaA.00654.b ) 1 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5392
RefSeq: YP_001853292.1
UniProt: B2HI37

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGNPVIVEAT RSPIGKRNGW LSGLHATELL GAVQKALVHK AGLSSGLQPG DVEQVIGGCV TQFGEQSNNI SRVAWLTAGL PDHVGATTVD CQCGSGQQAN HLIAGLIAIG AIDIGIACGI EAMSRVGLGA NAGPDRSAIR AKSWDIDMPN QFEAAERIAK RRGITRQDID AFGLESQLRA KRAWDEHRFD REISPIEAPV LDEQKQPTSE LHMVSRDQGL RDTTMAGLSE LKPVMEGGIH TAGTSSQISD GAAAVLWMDE EKARALGLRP RARIVSQALV GAEPYYHLDG PVQSTAKVLE KAGMKMGDID IVEINEAFAS VVLSWARVHE PDMAKVNVNG GAIALGHPVG CTGSRLITTA LHELERTDQS TALITMCAGG ALSTGTILER I
NT Sequence
ATGGGTAACC CGGTAATCGT AGAGGCGACG CGCAGTCCAA TCGGCAAGCG CAACGGATGG CTGTCAGGCC TGCACGCGAC CGAGTTGTTG GGGGCGGTTC AGAAGGCATT GGTGCACAAG GCCGGGCTCT CGTCGGGTCT ACAACCCGGA GATGTCGAAC AGGTCATCGG CGGTTGCGTG ACGCAGTTCG GGGAGCAGTC CAACAACATC AGCCGAGTGG CGTGGCTGAC CGCCGGCCTT CCCGATCATG TCGGCGCCAC CACTGTCGAC TGCCAGTGCG GCAGCGGCCA GCAAGCCAAC CATTTGATCG CCGGCCTGAT CGCGATCGGG GCCATCGACA TCGGCATTGC CTGCGGGATC GAGGCGATGA GCCGGGTGGG TCTGGGCGCC AACGCCGGGC CGGACCGCTC CGCAATCCGG GCCAAGTCCT GGGACATCGA CATGCCGAAC CAGTTCGAGG CCGCCGAGCG CATCGCCAAG CGCCGCGGGA TCACCCGCCA AGACATCGAC GCGTTCGGAC TCGAATCCCA GTTGCGCGCC AAGCGCGCCT GGGACGAACA CCGCTTTGAT CGCGAAATCT CACCGATCGA GGCCCCAGTG CTCGATGAGC AGAAGCAGCC CACCAGCGAG CTGCACATGG TCTCTCGGGA CCAGGGTCTG CGCGACACCA CCATGGCGGG CTTGAGTGAG CTCAAGCCGG TAATGGAGGG CGGCATTCAC ACCGCGGGCA CGTCATCGCA GATTTCTGAC GGCGCGGCCG CCGTGTTGTG GATGGACGAG GAAAAGGCCA GGGCACTCGG TTTGCGCCCA CGCGCCCGCA TCGTCAGCCA GGCGCTCGTG GGCGCCGAGC CGTACTACCA CCTGGACGGG CCGGTGCAGT CGACGGCAAA GGTGCTCGAG AAGGCCGGGA TGAAGATGGG CGACATCGAC ATCGTGGAGA TCAACGAGGC TTTCGCATCG GTGGTGCTGT CCTGGGCGCG GGTTCACGAA CCCGACATGG CCAAGGTCAA CGTCAACGGC GGCGCGATCG CCCTGGGCCA CCCGGTGGGA TGCACCGGCA GCCGGTTGAT CACCACCGCA CTGCACGAGC TGGAACGCAC CGACCAGAGC ACCGCGCTGA TCACCATGTG CGCGGGCGGA GCCCTGTCCA CCGGCACCAT TCTCGAGCGA ATC
Details for MymaA.00654.b.A1.GE30054
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 71
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGNPVIVEA TRSPIGKRNG WLSGLHATEL LGAVQKALVH KAGLSSGLQP GDVEQVIGGC VTQFGEQSNN ISRVAWLTAG LPDHVGATTV DCQCGSGQQA NHLIAGLIAI GAIDIGIACG IEAMSRVGLG ANAGPDRSAI RAKSWDIDMP NQFEAAERIA KRRGITRQDI DAFGLESQLR AKRAWDEHRF DREISPIEAP VLDEQKQPTS ELHMVSRDQG LRDTTMAGLS ELKPVMEGGI HTAXRHRRFL TARPPCCGWT XKSQGTXFAP TRPHRQPGAR GRRXVLPPGX XGAVDGKXAR EXXXXXGXXX XXXXXXLXXR XXXXGRGXXX XXXXXXXXGX IXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
tttgtttanc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggtaa cccggtaatc gtagaggcga cgcgcagtcc aatcggcaag cgcaacggat ggctgtcagg cctgcacgcg accgagttgt tgggggcggt tcagaaggca ttggtgcaca aggccgggct ctcgtcgggt ctacaacccg gagatgtcga acaggtcatc ggcggttgcg tgacgcagtt cggggagcag tccaacaaca tcagccgagt ggcgtggctg accgccggcc ttcccgatca tgtcggcgcc accactgtcg actgccagtg cggcagcggc cagcaagcca accatttgat cgccggcctg atcgcgatcg gggccatcga catcggcatt gcctgcggga tcgaggcgat gagccgggtg ggtctgggcg ccaacgccgg gccggaccgc tccgcaatcc gggccaagtc ctgggacatc gacatgccga accagttcga ggccgccgag cgcatcgcca agcgccgcgg gatcacccgc caagacatcg acgcgttcgg actcgaatcc cagttgcgcg ccaagcgcgc ctgggacgaa caccgctttg atcgcgaaat ctcaccgatc gaggccccag tgctcgatga gcagaagcag cccaccagcg agctgcacat ggtctctcgg gaccagggtc tgcgcgacac caccatggcg ggcttgagtg agctcaagcc ggtaatggag ggcggcattc acaccgcnnn acgtcatcgc agatttctga cggcgcggcc gccgtgttgt ggatggacga ngaaaagcca gggcactcng tttgcgccca cgcgcccgca tcgtcagccn ggngctcgtg ggcgccgann ngtactacca cctggnnggg nnggtgcagt cgacggnaaa nnngctcgag aangcngggn tgannngggn nacntcnncn tcntggngat nannangctt tnnnatcggn ggngctntcn nggncgcggg nnnncnnncn annnnnnnnn nnannncaan gnnggnnnna tcnnnngnnn nnncgnnnnn ncnnnnnnnn nnnntnnccn nnnnnnnnnn nnnnncnnnn nnnnnngnnn nnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGNPVIVEA TRSPIGKRNG WLSGLHATEL LGAVQKALVH KAGLSSGLQP GDVEQVIGGC VTQFGEQSNN ISRVAWLTAG LPDHVGATTV DCQCGSGQQA NHLIAGLIAI GAIDIGIACG IEAMSRVGLG ANAGPDRSAI RAKSWDIDMP NQFEAAERIA KRRGITRQDI DAFGLESQLR AKRAWDEHRF DREISPIEAP VLDEQKQPTS ELHMVSRDQG LRDTTMAGLS ELKPVMEGGI HTAGTSSQIS DGAAAVLWMD EEKARALGLR PRARIVSQAL VGAEPYYHLD GPVQSTAKVL EKAGMKMGDI DIVEINEAFA SVVLSWARVH EPDMAKVNVN GGAIALGHPV GCTGSRLITT ALHELERTDQ STALITMCAG GALSTGTILE R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtaacccg gtaatcgtag aggcgacgcg cagtccaatc ggcaagcgca acggatggct gtcaggcctg cacgcgaccg agttgttggg ggcggttcag aaggcattgg tgcacaaggc cgggctctcg tcgggtctac aacccggaga tgtcgaacag gtcatcggcg gttgcgtgac gcagttcggg gagcagtcca acaacatcag ccgagtggcg tggctgaccg ccggccttcc cgatcatgtc ggcgccacca ctgtcgactg ccagtgcggc agcggccagc aagccaacca tttgatcgcc ggcctgatcg cgatcggggc catcgacatc ggcattgcct gcgggatcga ggcgatgagc cgggtgggtc tgggcgccaa cgccgggccg gaccgctccg caatccgggc caagtcctgg gacatcgaca tgccgaacca gttcgaggcc gccgagcgca tcgccaagcg ccgcgggatc acccgccaag acatcgacgc gttcggactc gaatcccagt tgcgcgccaa gcgcgcctgg gacgaacacc gctttgatcg cgaaatctca ccgatcgagg ccccagtgct cgatgagcag aagcagccca ccagcgagct gcacatggtc tctcgggacc agggtctgcg cgacaccacc atggcgggct tgagtgagct caagccggta atggagggcg gcattcacac cgcgggcacg tcatcgcaga tttctgacgg cgcggccgcc gtgttgtgga tggacgagga aaaggccagg gcactcggtt tgcgcccacg cgcccgcatc gtcagccagg cgctcgtggg cgccgagccg tactaccacc tggacgggcc ggtgcagtcg acggcaaagg tgctcgagaa ggccgggatg aagatgggcg acatcgacat cgtggagatc aacgaggctt tcgcatcggt ggtgctgtcc tgggcgcggg ttcacgaacc cgacatggcc aaggtcaacg tcaacggcgg cgcgatcgcc ctgggccacc cggtgggatg caccggcagc cggttgatca ccaccgcact gcacgagctg gaacgcaccg accagagcac cgcgctgatc accatgtgcg cgggcggagc cctgtccacc ggcaccattc tcgagcgaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa