MytuD.00448.a

nicotinic acid mononucleotide adenyltransferase

CENTER ID: MytuD.00448.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00448.a.B1.GE27162 Full length( MytuD.00448.a ) 1 211
MytuD.00448.a.B1.GE27583 Full length( MytuD.00448.a ) 1 211
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_131883
PATRIC ID: fig|83332.12.peg.2711
RefSeq: NP_216937.1
TubercuList: Rv2421c
MTB Network Portal: Rv2421c
UniProt: P9WJJ5
Orthologues for Rv2421c
Species % Identity % Coverage Clones Proteins Structures
avium 82 99 0 0 0
paratuberculosis 82 99 0 0 0
leprae 80 99 0 0 0
marinum 80 99 1 0 0
abscessus 75 91 1 1 4
smegmatis 74 94 1 0 0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGTFDPIHY GHLVAASEVA DLFDLDEVVF VPSGQPWQKG RQVSAAEHRY LMTVIATASN PRFSVSRVDI DRGGPTYTKD TLADLHALHP DSELYFTTGA DALASIMSWQ GWEELFELAR FVGVSRPGYE LRNEHITSLL GQLAKDALTL VEIPALAISS TDCRQRAEQS RPLWYLMPDG VVQYVSKCRL YCGACDAGAR STTSLAAGNG L
NT Sequence
ATGGGTGGGA CGTTCGACCC CATCCACTAC GGCCACCTGG TTGCCGCCAG CGAGGTGGCC GACCTGTTCG ATCTCGACGA AGTGGTATTC GTGCCCAGCG GGCAACCCTG GCAAAAGGGT CGACAGGTCT CCGCCGCCGA GCACCGCTAC CTAATGACGG TGATCGCCAC CGCCTCCAAT CCCCGATTCT CTGTGAGCCG GGTCGACATC GACCGCGGCG GACCCACCTA CACCAAGGAC ACGCTGGCCG ATCTGCATGC TTTGCACCCG GACTCTGAGC TGTACTTCAC CACCGGCGCC GATGCGCTAG CTTCCATAAT GTCCTGGCAG GGCTGGGAGG AGCTGTTCGA ATTGGCGCGG TTCGTGGGGG TCAGCCGGCC CGGCTACGAG TTGCGCAACG AACACATCAC TAGCCTGCTG GGTCAGCTGG CCAAGGATGC GTTGACTCTG GTCGAGATCC CGGCGCTGGC CATTTCGTCG ACCGACTGCC GTCAGCGCGC CGAGCAGTCC CGGCCGCTGT GGTACCTGAT GCCCGACGGC GTCGTGCAGT ATGTCTCCAA GTGCCGGCTC TACTGTGGCG CCTGCGACGC GGGCGCGCGT TCAACGACCA GCCTGGCCGC TGGGAATGGC CTA
Details for MytuD.00448.a.B1.GE27162
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 24kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM GGTFDPIHYG HLVAASEVAD LFDLDEVVFV PSGQPWQKGR QVSAAEHRYL MTVIATASNP RFSVSRVDID RGGPTYTKDT LADLHALHPD SELYFTTGAD ALASIMSWQG WEELFELARF VGVSRPGYEL RNEHITSLLG QLAKDALTLV EIPALAISST DCRQRAEQSR PLWYLMPDGV VQYVSKCRLY CGACDAGARS TTSLAAGNGL
Validated NT Sequence
atggctcacc accaccacca ccaccatatg ggtgggacgt tcgaccccat ccactacggc cacctggttg ccgccagcga ggtggccgac ctgttcgatc tcgacgaagt ggtattcgtg cccagcgggc aaccctggca aaagggtcga caggtctccg ccgccgagca ccgctaccta atgacggtga tcgccaccgc ctccaatccc cgattctctg tgagccgggt cgacatcgac cgcggcggac ccacctacac caaggacacg ctggccgatc tgcatgcttt gcacccggac tctgagctgt acttcaccac cggcgccgat gcgctagctt ccataatgtc ctggcagggc tgggaggagc tgttcgaatt ggcgcggttc gtgggggtca gccggcccgg ctacgagttg cgcaacgaac acatcactag cctgctgggt cagctggcca aggatgcgtt gactctggtc gagatcccgg cgctggccat ttcgtcgacc gactgccgtc agcgcgccga gcagtcccgg ccgctgtggt acctgatgcc cgacggcgtc gtgcagtatg tctccaagtg ccggctctac tgtggcgcct gcgacgcggg cgcgcgttca acgaccagcc tggccgctgg gaatggccta taagtgagta agat
Expected Protein Sequence
MAHHHHHHMG GTFDPIHYGH LVAASEVADL FDLDEVVFVP SGQPWQKGRQ VSAAEHRYLM TVIATASNPR FSVSRVDIDR GGPTYTKDTL ADLHALHPDS ELYFTTGADA LASIMSWQGW EELFELARFV GVSRPGYELR NEHITSLLGQ LAKDALTLVE IPALAISSTD CRQRAEQSRP LWYLMPDGVV QYVSKCRLYC GACDAGARST TSLAAGNGL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtg ggacgttcga ccccatccac tacggccacc tggttgccgc cagcgaggtg gccgacctgt tcgatctcga cgaagtggta ttcgtgccca gcgggcaacc ctggcaaaag ggtcgacagg tctccgccgc cgagcaccgc tacctaatga cggtgatcgc caccgcctcc aatccccgat tctctgtgag ccgggtcgac atcgaccgcg gcggacccac ctacaccaag gacacgctgg ccgatctgca tgctttgcac ccggactctg agctgtactt caccaccggc gccgatgcgc tagcttccat aatgtcctgg cagggctggg aggagctgtt cgaattggcg cggttcgtgg gggtcagccg gcccggctac gagttgcgca acgaacacat cactagcctg ctgggtcagc tggccaagga tgcgttgact ctggtcgaga tcccggcgct ggccatttcg tcgaccgact gccgtcagcg cgccgagcag tcccggccgc tgtggtacct gatgcccgac ggcgtcgtgc agtatgtctc caagtgccgg ctctactgtg gcgcctgcga cgcgggcgcg cgttcaacga ccagcctggc cgctgggaat ggcctatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for MytuD.00448.a.B1.GE27583
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 24kDa
OBSERVED MW: 24kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM GGTFDPIHYG HLVAASEVAD LFDLDEVVFV PSGQPWQKGR QVSAAEHRYL MTVIATASNP RFSVSRVDID RGGPTYTKDT LADLHALHPD SELYFTTGAD ALASIMSWQG WEELFELARF VGVSRPGYEL RNEHITSLLG QLAKDALTLV EIPALAISST DCRQRAEQSR PLWYLMPDGV VQYVSKCRLY CGACDAGARS TTSLAAGNGL
Validated NT Sequence
atggctcacc accaccacca ccaccatatg ggtgggacgt tcgaccccat ccactacggc cacctggttg ccgccagcga ggtggccgac ctgttcgatc tcgacgaagt ggtattcgtg cccagcgggc aaccctggca aaagggtcga caggtctccg ccgccgagca ccgctaccta atgacggtga tcgccaccgc ctccaatccc cgattctctg tgagccgggt cgacatcgac cgcggcggac ccacctacac caaggacacg ctggccgatc tgcatgcttt gcacccggac tctgagctgt acttcaccac cggcgccgat gcgctagctt ccataatgtc ctggcagggc tgggaggagc tgttcgaatt ggcgcggttc gtgggggtca gccggcccgg ctacgagttg cgcaacgaac acatcactag cctgctgggt cagctggcca aggatgcgtt gactctggtc gagatcccgg cgctggccat ttcgtcgacc gactgccgtc agcgcgccga gcagtcccgg ccgctgtggt acctgatgcc cgacggcgtc gtgcagtatg tctccaagtg ccggctctac tgtggcgcct gcgacgcggg cgcgcgttca acgaccagcc tggccgctgg gaatggccta taagtgagta agat
Expected Protein Sequence
MAHHHHHHMG GTFDPIHYGH LVAASEVADL FDLDEVVFVP SGQPWQKGRQ VSAAEHRYLM TVIATASNPR FSVSRVDIDR GGPTYTKDTL ADLHALHPDS ELYFTTGADA LASIMSWQGW EELFELARFV GVSRPGYELR NEHITSLLGQ LAKDALTLVE IPALAISSTD CRQRAEQSRP LWYLMPDGVV QYVSKCRLYC GACDAGARST TSLAAGNGL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtg ggacgttcga ccccatccac tacggccacc tggttgccgc cagcgaggtg gccgacctgt tcgatctcga cgaagtggta ttcgtgccca gcgggcaacc ctggcaaaag ggtcgacagg tctccgccgc cgagcaccgc tacctaatga cggtgatcgc caccgcctcc aatccccgat tctctgtgag ccgggtcgac atcgaccgcg gcggacccac ctacaccaag gacacgctgg ccgatctgca tgctttgcac ccggactctg agctgtactt caccaccggc gccgatgcgc tagcttccat aatgtcctgg cagggctggg aggagctgtt cgaattggcg cggttcgtgg gggtcagccg gcccggctac gagttgcgca acgaacacat cactagcctg ctgggtcagc tggccaagga tgcgttgact ctggtcgaga tcccggcgct ggccatttcg tcgaccgact gccgtcagcg cgccgagcag tcccggccgc tgtggtacct gatgcccgac ggcgtcgtgc agtatgtctc caagtgccgg ctctactgtg gcgcctgcga cgcgggcgcg cgttcaacga ccagcctggc cgctgggaat ggcctatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t