MymaA.00448.a

Probable nicotinate-nucleotide adenylyltransferase

CENTER ID:	MymaA.00448.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00448.a.A1.GE34938	Full length( MymaA.00448.a )		1	215

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_131883
PATRIC ID:	fig\|216594.6.peg.4008
RefSeq:	YP_001852020.1
UniProt:	B2HME5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGGTFDPIHY GHLVAASEVA DRYELDEVVF VPSGQPWQKG RRVSPAEDRY LMTVIATASN PRFSVSRVDI DRGGPTYTKD TLRDLHALNP AAELYFITGA DALASIMSWQ GWEQMFESAR FVGVSRPGYE LRHDHVTAAL DGLAEDALSL VEIPALAISS TDCRRRAAHG RPLWYLMPDG VVQYVSKRRL YRTPPDSGGG ETLDTTTILP AGNNT

NT Sequence

ATGGGTGGGA CTTTCGATCC CATCCATTAC GGTCACCTGG TCGCCGCCAG TGAGGTAGCC GACCGCTACG AACTCGACGA GGTCGTGTTC GTGCCCAGCG GACAGCCGTG GCAGAAGGGA CGGCGTGTTT CGCCTGCCGA AGACCGCTAT CTGATGACGG TCATCGCGAC GGCTTCCAAC CCCCGCTTTT CGGTCAGCCG GGTCGACATC GACCGGGGCG GTCCGACCTA CACCAAGGAC ACGCTGCGGG ATCTGCATGC CCTCAACCCG GCTGCGGAGC TGTACTTCAT CACCGGCGCC GACGCCCTGG CCTCGATCAT GTCCTGGCAG GGCTGGGAGC AGATGTTTGA GTCGGCGCGG TTTGTCGGAG TGAGTCGGCC GGGCTACGAA CTGCGCCACG ACCATGTCAC CGCCGCGCTG GACGGGCTCG CCGAGGACGC CTTGTCCCTG GTGGAGATCC CGGCGCTGGC GATCTCATCG ACGGATTGCC GTCGGCGCGC CGCGCATGGT CGCCCCCTGT GGTACCTGAT GCCCGACGGC GTCGTGCAGT ATGTCTCAAA GCGCAGGCTC TACCGCACGC CACCGGACTC GGGGGGCGGG GAGACCCTGG ACACCACGAC CATTCTGCCC GCGGGGAACA ACACA

Details for MymaA.00448.a.A1.GE34938

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGGTFDPIH YGHLVAASEV ADRYELDEVV FVPSGQPWQK GRRVSPAEDR YLMTVIATAS NPRFSVSRVD IDRGGPTYTK DTLRDLHALN PAAELYFITG ADALASIMSW QGWEQMFESA RFVGVSRPGY ELRHDHVTAA LDGLAEDALS LVEIPALAIS STDCRRRAAH GRPLWYLMPD GVVQYVSKRR LYRTPPDSGG GETLDTTTIL PAG

Validated NT Sequence

tccccgcggg cagaatggtc gtggtgtcca gggtctcccc gccccccgag tccggtggcg tgcggtagag cctgcgcttt gagacatact gcacgacgcc gtcgggcatc aggtaccaca gggggcgacc atgcgcggcg cgccgacggc aatccgtcga tgagatcgcc agcgccggga tctccaccag ggacaaggcg tcctcggcga gcccgtccag cgcggcggtg acatggtcgt ggcgcagttc gtagcccggc cgactcactc cgacaaaccg cgccgactca aacatctgct cccagccctg ccaggacatg atcgaggcca gggcgtcggc gccggtgatg aagtacagct ccgcagccgg gttgagggca tgcagatccc gcagcgtgtc cttggtgtag gtcggaccgc cccggtcgat gtcgacccgg ctgaccgaaa agcgggggtt ggaagccgtc gcgatgaccg tcatcagata gcggtcttcg gcaggcgaaa cacgccgtcc cttctgccac ggctgtccgc tgggcacgaa cacgacctcg tcgagttcgt agcggtcggc tacctcactg gcggcgacca ggtgaccgta atggatggga tcgaaagtcc cacccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtgggact ttcgatccca tccattacgg tcacctggtc gccgccagtg aggtagccga ccgctacgaa ctcgacgagg tcgtgttcgt gcccagcgga cagccgtggc agaagggacg gcgtgtttcg cctgccgaag accgctatct gatgacggtc atcgcgacgg cttccaaccc ccgcttttcg gtcagccggg tcgacatcga ccggggcggt ccgacctaca ccaaggacac gctgcgggat ctgcatgccc tcaacccggc tgcggagctg tacttcatca ccggcgccga cgccctggcc tcgatcatgt cctggcaggg ctgggagcag atgtttgagt cggcgcggtt tgtcggagtg agtcggccgg gctacgaact gcgccacgac catgtcaccg ccgcgctgga cgggctcgcc gaggacgcct tgtccctggt ggagatcccg gcgctggcga tctcatcgac ggattgccgt cggcgcgccg cgcatggtcg ccccctgtgg tacctgatgc ccgacggcgt cgtgcagtat gtctcaaagc gcaggctcta ccgcacgcca ccggactcgg ggggcgggga gaccctggac accacgacca ttctgcccgc ggggaacaac aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa