MyabA.00448.a
Probable nicotinate-nucleotide adenylyltransferase
| CENTER ID: | MyabA.00448.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00448.a.A1.GE30305 | Full length( MyabA.00448.a ) | 1 | 211 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00448.a.A1.PS00938 | Full length( MyabA.00448.a ) | 1 | 211 |
Structures
4YMI
DEPOSITED: 3/6/2015
DETERMINATION: XRay
CLONE: MyabA.00448.a.A1.GE30305
PROTEIN: MyabA.00448.a.A1.PS00938
5DB4
DEPOSITED: 8/20/2015
DETERMINATION: XRay
CLONE: MyabA.00448.a.A1.GE30305
PROTEIN: MyabA.00448.a.A1.PS00938
5DEO
DEPOSITED: 8/25/2015
DETERMINATION: XRay
CLONE: MyabA.00448.a.A1.GE30305
PROTEIN: MyabA.00448.a.A1.PS00938
5VIR
DEPOSITED: 4/17/2017
DETERMINATION: XRay
CLONE: MyabA.00448.a.A1.GE30305
PROTEIN: MyabA.00448.a.A1.PS00938
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131883 |
| PATRIC ID: | fig|36809.5.peg.1661 |
| RefSeq: | YP_001702360.1 |
| UniProt: | B1MMZ4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQSERRRLGV MGGTFDPIHN GHLVAASEVA DRFALDEVIF VPTGQPWQKQ GRKVSPAEHR YLMTVIATAS NPRFTVSRAD IDRGGATYTV DTLTDLRTAH PDADLYFITG ADALASILSW ENWEQLFTLA KFIGVSRPGY ELSSDHIAHA ELPPDGLSLV EVPALAISST DCRIRAGQAR PIWYLVPDGV VQYVAKHRLY SGNKGNQGGL A
NT Sequence
GTGCAATCTG AGCGACGCAG ACTTGGGGTG ATGGGTGGAA CCTTCGATCC CATCCACAAC GGTCACTTGG TTGCCGCCAG CGAGGTCGCC GATCGGTTCG CACTCGACGA GGTCATCTTC GTGCCGACGG GGCAGCCGTG GCAGAAGCAG GGACGCAAGG TCAGCCCCGC CGAGCACCGC TATCTGATGA CCGTGATCGC CACCGCGTCG AATCCGCGAT TCACCGTCAG CCGCGCCGAT ATAGATCGCG GCGGCGCGAC CTATACCGTG GACACCCTCA CCGATCTGCG GACCGCCCAC CCGGATGCCG ACCTGTACTT CATCACCGGG GCGGATGCAC TGGCATCGAT CTTGTCGTGG GAGAACTGGG AGCAGCTGTT CACGCTGGCC AAGTTCATCG GGGTCAGCCG ACCCGGGTAC GAACTGAGCT CCGATCACAT CGCCCATGCC GAACTCCCGC CCGACGGGTT GTCACTTGTC GAGGTGCCCG CGCTCGCCAT CTCGTCGACC GACTGCCGGA TTCGGGCGGG CCAGGCACGG CCGATCTGGT ACCTGGTGCC CGACGGTGTC GTGCAGTACG TCGCAAAACA CCGCCTTTAC AGCGGAAACA AAGGAAATCA AGGAGGCCTC GCA
Details for MyabA.00448.a.A1.GE30305
| HARVESTED ON: | 9/8/2010 |
| SEQUENCED ON: | 2/17/2015 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVQSERRRL GVMGGTFDPI HNGHLVAASE VADRFALDEV IFVPTGQPWQ KQGRKVSPAE HRYLMTVIAT ASNPRFTVSR ADIDRGGATY TVDTLTDLRT AHPDADLYFI TGADALASIL SWENWEQLFT LAKFIGVSRP GYELSSDHIA HAELPPDGLS LVEVPALAIS STDCRIRAGQ ARPIWYLVPD GVVQYVAKHR LYSGNKGNQG GLA
Validated NT Sequence
ttattatgcg aggcctcctt gatttccttt gtttccgctg taaaggcggt gttttgcgac gtactgcacg acaccgtcgg gcaccaggta ccagatcggc cgtgcctggc ccgcccgaat ccggcagtcg gtcgacgaga tggcgagcgc gggcacctcg acaagtgaca acccgtcggg cgggagttcg gcatgggcga tgtgatcgga gctcagttcg tacccgggtc ggctgacccc gatgaacttg gccagcgtga acagctgctc ccagttctcc cacgacaaga tcgatgccag tgcatccgcc ccggtgatga agtacaggtc ggcatccggg tgggcggtcc gcagatcggt gagggtgtcc acggtatagg tcgcgccgcc gcgatctata tcggcgcggc tgacggtgaa tcgcggattc gacgcggtgg cgatcacggt catcagatag cggtgctcgg cggggctgac cttgcgtccc tgcttctgcc acggctgccc cgtcggcacg aagatgacct cgtcgagtgc gaaccgatcg gcgacctcgc tggcggcaac caagtgaccg ttgtggatgg gatcgaaggt tccacccatc accccaagtc tgcgtcgctc agattgcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQSERRRL GVMGGTFDPI HNGHLVAASE VADRFALDEV IFVPTGQPWQ KQGRKVSPAE HRYLMTVIAT ASNPRFTVSR ADIDRGGATY TVDTLTDLRT AHPDADLYFI TGADALASIL SWENWEQLFT LAKFIGVSRP GYELSSDHIA HAELPPDGLS LVEVPALAIS STDCRIRAGQ ARPIWYLVPD GVVQYVAKHR LYSGNKGNQG GLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcaatct gagcgacgca gacttggggt gatgggtgga accttcgatc ccatccacaa cggtcacttg gttgccgcca gcgaggtcgc cgatcggttc gcactcgacg aggtcatctt cgtgccgacg gggcagccgt ggcagaagca gggacgcaag gtcagccccg ccgagcaccg ctatctgatg accgtgatcg ccaccgcgtc gaatccgcga ttcaccgtca gccgcgccga tatagatcgc ggcggcgcga cctataccgt ggacaccctc accgatctgc ggaccgccca cccggatgcc gacctgtact tcatcaccgg ggcggatgca ctggcatcga tcttgtcgtg ggagaactgg gagcagctgt tcacgctggc caagttcatc ggggtcagcc gacccgggta cgaactgagc tccgatcaca tcgcccatgc cgaactcccg cccgacgggt tgtcacttgt cgaggtgccc gcgctcgcca tctcgtcgac cgactgccgg attcgggcgg gccaggcacg gccgatctgg tacctggtgc ccgacggtgt cgtgcagtac gtcgcaaaac accgccttta cagcggaaac aaaggaaatc aaggaggcct cgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyabA.00448.a.A1.PS00938
| PURIFICATION DATe: | 1/13/2011 |
| CONCENTRATION: | 46.62mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVQSERRRL GVMGGTFDPI HNGHLVAASE VADRFALDEV IFVPTGQPWQ KQGRKVSPAE HRYLMTVIAT ASNPRFTVSR ADIDRGGATY TVDTLTDLRT AHPDADLYFI TGADALASIL SWENWEQLFT LAKFIGVSRP GYELSSDHIA HAELPPDGLS LVEVPALAIS STDCRIRAGQ ARPIWYLVPD GVVQYVAKHR LYSGNKGNQG GLA
Validated NT Sequence
ttattatgcg aggcctcctt gatttccttt gtttccgctg taaaggcggt gttttgcgac gtactgcacg acaccgtcgg gcaccaggta ccagatcggc cgtgcctggc ccgcccgaat ccggcagtcg gtcgacgaga tggcgagcgc gggcacctcg acaagtgaca acccgtcggg cgggagttcg gcatgggcga tgtgatcgga gctcagttcg tacccgggtc ggctgacccc gatgaacttg gccagcgtga acagctgctc ccagttctcc cacgacaaga tcgatgccag tgcatccgcc ccggtgatga agtacaggtc ggcatccggg tgggcggtcc gcagatcggt gagggtgtcc acggtatagg tcgcgccgcc gcgatctata tcggcgcggc tgacggtgaa tcgcggattc gacgcggtgg cgatcacggt catcagatag cggtgctcgg cggggctgac cttgcgtccc tgcttctgcc acggctgccc cgtcggcacg aagatgacct cgtcgagtgc gaaccgatcg gcgacctcgc tggcggcaac caagtgaccg ttgtggatgg gatcgaaggt tccacccatc accccaagtc tgcgtcgctc agattgcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQSERRRL GVMGGTFDPI HNGHLVAASE VADRFALDEV IFVPTGQPWQ KQGRKVSPAE HRYLMTVIAT ASNPRFTVSR ADIDRGGATY TVDTLTDLRT AHPDADLYFI TGADALASIL SWENWEQLFT LAKFIGVSRP GYELSSDHIA HAELPPDGLS LVEVPALAIS STDCRIRAGQ ARPIWYLVPD GVVQYVAKHR LYSGNKGNQG GLA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCAATCT GAGCGACGCA GACTTGGGGT GATGGGTGGA ACCTTCGATC CCATCCACAA CGGTCACTTG GTTGCCGCCA GCGAGGTCGC CGATCGGTTC GCACTCGACG AGGTCATCTT CGTGCCGACG GGGCAGCCGT GGCAGAAGCA GGGACGCAAG GTCAGCCCCG CCGAGCACCG CTATCTGATG ACCGTGATCG CCACCGCGTC GAATCCGCGA TTCACCGTCA GCCGCGCCGA TATAGATCGC GGCGGCGCGA CCTATACCGT GGACACCCTC ACCGATCTGC GGACCGCCCA CCCGGATGCC GACCTGTACT TCATCACCGG GGCGGATGCA CTGGCATCGA TCTTGTCGTG GGAGAACTGG GAGCAGCTGT TCACGCTGGC CAAGTTCATC GGGGTCAGCC GACCCGGGTA CGAACTGAGC TCCGATCACA TCGCCCATGC CGAACTCCCG CCCGACGGGT TGTCACTTGT CGAGGTGCCC GCGCTCGCCA TCTCGTCGAC CGACTGCCGG ATTCGGGCGG GCCAGGCACG GCCGATCTGG TACCTGGTGC CCGACGGTGT CGTGCAGTAC GTCGCAAAAC ACCGCCTTTA CAGCGGAAAC AAAGGAAATC AAGGAGGCCT CGCATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA