MytuD.00010.a
3-ketoacyl-(acyl-carrier-protein) reductase
| CENTER ID: | MytuD.00010.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00010.a.A1.GE25247 | Full length( MytuD.00010.a ) | 1 | 454 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3LLS
DEPOSITED: 1/29/2010
DETERMINATION: XRay
CLONE: MytuD.00010.a.A1.GE25247
PROTEIN: MytuD.00010.a.A1.PW25285
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_187141 |
| PATRIC ID: | fig|83332.12.peg.272 |
| RefSeq: | NP_214756.1 |
| TubercuList: | Rv0242c |
| MTB Network Portal: | Rv0242c |
| UniProt: | I6Y778 |
Orthologues for Rv0242c
| Species | % Identity | % Coverage | Clones | Proteins | Structures |
|---|---|---|---|---|---|
| avium | 90 | 100 | 0 | 0 | 0 |
| paratuberculosis | 90 | 100 | 1 | 0 | 0 |
| marinum | 88 | 100 | 0 | 0 | 0 |
| ulcerans | 87 | 100 | 1 | 0 | 0 |
| leprae | 85 | 100 | 0 | 0 | 0 |
| smegmatis | 84 | 99 | 1 | 0 | 2 |
| thermoresistibile | 81 | 52 | 0 | 0 | 0 |
| abscessus | 78 | 100 | 0 | 0 | 0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPKRSSDLF SQVVNSGPGS FLARQLGVPQ PETLRRYRAG EPPLTGSLLI GGAGRVVEPL RAALEKDYDL VGNNLGGRWA DSFGGLVFDA TGITEPAGLK GLHEFFTPVL RNLGRCGRVV VVGGTPEAAA STNERIAQRA LEGFTRSLGK ELRRGATTAL VYLSPDAKPA ATGLESTMRF LLSAKSAYVD GQVFSVGADD STPPADWEKP LDGKVAIVTG AARGIGATIA EVFARDGAHV VAIDVESAAE NLAETASKVG GTALWLDVTA DDAVDKISEH LRDHHGGKAD ILVNNAGITR DKLLANMDDA RWDAVLAVNL LAPLRLTEGL VGNGSIGEGG RVIGLSSIAG IAGNRGQTNY ATTKAGMIGI TQALAPGLAA KGITINAVAP GFIETQMTAA IPLATREVGR RLNSLLQGGQ PVDVAEAIAY FASPASNAVT GNVIRVCGQA MIGA
NT Sequence
GTGGCTCCCA AGCGTTCGTC CGATCTGTTC TCGCAGGTTG TCAACTCCGG TCCAGGATCG TTTTTGGCCA GGCAATTGGG TGTTCCGCAA CCCGAGACGC TTCGCCGCTA TCGAGCAGGC GAACCGCCAC TGACCGGGTC GCTGCTGATC GGCGGGGCCG GCAGGGTCGT CGAACCACTG CGCGCGGCAC TCGAAAAGGA CTACGACCTG GTAGGAAACA ACCTGGGCGG GCGCTGGGCG GACTCGTTCG GCGGGCTGGT CTTCGACGCC ACCGGCATCA CAGAGCCGGC CGGGCTCAAG GGGCTGCACG AGTTCTTTAC TCCGGTACTG CGCAATCTCG GCCGTTGCGG GCGCGTGGTG GTTGTTGGCG GCACACCCGA AGCGGCCGCC AGCACGAACG AGCGGATCGC GCAGCGCGCG CTGGAGGGCT TCACCCGCTC GCTGGGCAAG GAACTGCGCC GCGGTGCGAC AACGGCGTTG GTCTACCTGT CGCCGGACGC CAAGCCCGCA GCTACCGGCC TCGAGTCGAC CATGCGATTC CTGCTGTCGG CCAAGTCGGC CTACGTCGAC GGCCAAGTCT TCTCTGTCGG GGCGGACGAT TCCACACCGC CGGCCGACTG GGAGAAGCCG CTGGACGGCA AGGTCGCCAT TGTGACCGGC GCGGCCCGCG GCATCGGCGC AACCATCGCC GAGGTGTTCG CTCGCGACGG CGCCCACGTG GTCGCCATCG ATGTGGAGTC CGCCGCCGAG AACCTGGCCG AAACCGCCAG CAAGGTGGGC GGCACCGCGC TGTGGCTCGA CGTCACCGCC GACGATGCCG TCGACAAGAT CAGCGAGCAC CTGCGCGACC ACCACGGTGG CAAGGCCGAC ATCCTGGTCA ACAACGCCGG CATCACCCGT GACAAGCTGC TGGCCAACAT GGACGATGCG CGTTGGGACG CCGTCCTGGC TGTCAATCTG CTTGCCCCGT TGCGGCTCAC CGAGGGTCTG GTGGGCAACG GCAGCATCGG AGAGGGTGGT CGGGTGATCG GGCTGTCGTC GATCGCCGGC ATCGCCGGCA ACCGCGGCCA GACCAACTAC GCCACCACCA AGGCCGGGAT GATCGGCATC ACCCAGGCGC TGGCACCCGG CCTGGCCGCG AAAGGCATCA CGATCAACGC CGTGGCACCG GGATTCATCG AAACCCAGAT GACGGCCGCC ATCCCGCTGG CCACCCGTGA GGTAGGCCGC CGGCTGAACT CGCTGCTGCA GGGCGGGCAG CCCGTCGACG TCGCCGAGGC CATCGCCTAC TTCGCCAGCC CGGCGTCAAA CGCGGTGACC GGCAACGTCA TTCGTGTCTG CGGCCAGGCC ATGATCGGCG CG
Details for MytuD.00010.a.A1.GE25247
| HARVESTED ON: | 5/6/2008 |
| SEQUENCED ON: | 6/16/2009 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 65 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAPKRSSD LFSQVVNSGP GSFLARQLGV PQPETLRRYR AGEPPLTGSL LIGGAGRVVE PLRAALEKDY DLVGNNLGGR WADSFGGLVF DATGITEPAG LKGLHEFFTP VLRNLGRCGR VVVVGGTPEA AASTNERIAQ RALEGFTRSL GKELRRGATT ALVYLSPDAK PAATGLESTM RFLLSAKSAY VDGQVFSVGA DDSTPPADWE KPLDGKVAIV TGAARGIGAT IAXVFARDGA HVVAIDVXSA AXXLAETASK VGGTALWLDV TADDAVXXXS EXXRDHHGGK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg ctcccaagcg ttcgtccgat ctgttctcgc aggttgtcaa ctccggtcca ggatcgtttt tggccaggca attgggtgtt ccgcaacccg agacgcttcg ccgctatcga gcaggcgaac cgccactgac cgggtcgctg ctgatcggcg gggccggcag ggtcgtcgaa ccactgcgcg cggcactcga aaaggactac gacctggtag gaaacaacct gggcgggcgc tgggcggact cgttcggcgg gctggtcttc gacgccaccg gcatcacaga gccggccggg ctcaaggggc tgcacgagtt ctttactccg gtactgcgca atctcggccg ttgcgggcgc gtggtggttg ttggcggcac acccgaagcg gccgccagca cgaacgagcg gatcgcgcag cgcgcgctgg agggcttcac ccgctcgctg ggcaaggaac tgcgccgcgg tgcgacaacg gcgttggtct acctgtcgcc ggacgccaag cccgcagcta ccggcctcga gtcgaccatg cgattcctgc tgtcggccaa gtcggcctac gtcgacggcc aagtcttctc tgtcggggcg gacgattcca caccgccggc cgactgggag aagccgctgg acggcaaggt cgccattgtg accggcgcgg cccgcggcat cggcgcaacc atcgccgang tgttcgctcg cgacggcgcc cacgtggtcg ccatcgatgt gnagtccgcc gccganaanc tggccgaaac cgccagcaag gtgggcggna ccgcgctgtg gctcgacgtc accgccgacg atgccgtcna nannntcagc gagcanntgc gcgaccacca cggtggcaag g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAPKRSSD LFSQVVNSGP GSFLARQLGV PQPETLRRYR AGEPPLTGSL LIGGAGRVVE PLRAALEKDY DLVGNNLGGR WADSFGGLVF DATGITEPAG LKGLHEFFTP VLRNLGRCGR VVVVGGTPEA AASTNERIAQ RALEGFTRSL GKELRRGATT ALVYLSPDAK PAATGLESTM RFLLSAKSAY VDGQVFSVGA DDSTPPADWE KPLDGKVAIV TGAARGIGAT IAEVFARDGA HVVAIDVESA AENLAETASK VGGTALWLDV TADDAVDKIS EHLRDHHGGK ADILVNNAGI TRDKLLANMD DARWDAVLAV NLLAPLRLTE GLVGNGSIGE GGRVIGLSSI AGIAGNRGQT NYATTKAGMI GITQALAPGL AAKGITINAV APGFIETQMT AAIPLATREV GRRLNSLLQG GQPVDVAEAI AYFASPASNA VTGNVIRVCG QAMIGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggctccc aagcgttcgt ccgatctgtt ctcgcaggtt gtcaactccg gtccaggatc gtttttggcc aggcaattgg gtgttccgca acccgagacg cttcgccgct atcgagcagg cgaaccgcca ctgaccgggt cgctgctgat cggcggggcc ggcagggtcg tcgaaccact gcgcgcggca ctcgaaaagg actacgacct ggtaggaaac aacctgggcg ggcgctgggc ggactcgttc ggcgggctgg tcttcgacgc caccggcatc acagagccgg ccgggctcaa ggggctgcac gagttcttta ctccggtact gcgcaatctc ggccgttgcg ggcgcgtggt ggttgttggc ggcacacccg aagcggccgc cagcacgaac gagcggatcg cgcagcgcgc gctggagggc ttcacccgct cgctgggcaa ggaactgcgc cgcggtgcga caacggcgtt ggtctacctg tcgccggacg ccaagcccgc agctaccggc ctcgagtcga ccatgcgatt cctgctgtcg gccaagtcgg cctacgtcga cggccaagtc ttctctgtcg gggcggacga ttccacaccg ccggccgact gggagaagcc gctggacggc aaggtcgcca ttgtgaccgg cgcggcccgc ggcatcggcg caaccatcgc cgaggtgttc gctcgcgacg gcgcccacgt ggtcgccatc gatgtggagt ccgccgccga gaacctggcc gaaaccgcca gcaaggtggg cggcaccgcg ctgtggctcg acgtcaccgc cgacgatgcc gtcgacaaga tcagcgagca cctgcgcgac caccacggtg gcaaggccga catcctggtc aacaacgccg gcatcacccg tgacaagctg ctggccaaca tggacgatgc gcgttgggac gccgtcctgg ctgtcaatct gcttgccccg ttgcggctca ccgagggtct ggtgggcaac ggcagcatcg gagagggtgg tcgggtgatc gggctgtcgt cgatcgccgg catcgccggc aaccgcggcc agaccaacta cgccaccacc aaggccggga tgatcggcat cacccaggcg ctggcacccg gcctggccgc gaaaggcatc acgatcaacg ccgtggcacc gggattcatc gaaacccaga tgacggccgc catcccgctg gccacccgtg aggtaggccg ccggctgaac tcgctgctgc agggcgggca gcccgtcgac gtcgccgagg ccatcgccta cttcgccagc ccggcgtcaa acgcggtgac cggcaacgtc attcgtgtct gcggccaggc catgatcggc gcgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa