MypaA.00010.a

FabG4

CENTER ID: MypaA.00010.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00010.a.A1.GE29178 Full length( MypaA.00010.a ) 1 454
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.3930
RefSeq: NP_962626.1
UniProt: Q73TM6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPKVSSDLF SQIVNSGPGS FLAKQLGVPQ PETLRRYRPG DPPLAGSLLI GGEGRVVEPL RAALAKDYDL VGNNLGGRWA DRFGGLVFDA TGITTPEGLK GLYEFFTPLL RNLGHCARVV VVGTTPDAAA GPHERIAQRA LEGFTRSLGK ELRNGSTVAL VYLSPAAKPA ATGLESTMRF ILSAKSAYVD GQVFYVGEAD STPPADWERP LDGKVAIVTG AARGIGATIA EVFARDGARV VAIDVESAAE TLAETASRVG GTALWLDVTA PDAVDKITEH LREHHGGHAD ILVNNAGITR DKLLANMDDA RWDAVLAVNL LAPLRLTEGL VGNGSIGEGG RIVGLSSMAG IAGNRGQTNY ATTKAGMIGL TQALAPELYD KGITINAVAP GFIETQMTAA IPLATREVGR RMNSLLQGGQ PVDVAETIAY FASPASNAVT GNVIRVCGQA MLGA
NT Sequence
GTGGCTCCGA AGGTCTCGTC CGATCTGTTC TCGCAGATTG TCAATTCCGG TCCTGGATCG TTTCTCGCCA AGCAGCTCGG CGTCCCGCAA CCCGAGACGC TGCGCCGCTA CCGGCCCGGT GACCCGCCGC TGGCCGGGTC GCTGCTGATC GGCGGCGAGG GCCGCGTGGT CGAGCCGCTG CGGGCGGCGC TGGCCAAGGA CTACGACCTG GTCGGCAACA ACCTGGGCGG GCGCTGGGCC GACCGGTTCG GCGGGCTGGT CTTCGACGCC ACCGGGATCA CCACCCCGGA GGGCCTGAAG GGGCTGTACG AGTTCTTCAC CCCACTGCTG CGCAACCTGG GTCACTGCGC CCGCGTGGTG GTGGTCGGCA CCACGCCCGA CGCCGCCGCC GGCCCGCACG AGCGGATCGC CCAGCGCGCC CTGGAGGGCT TCACCCGGTC ATTGGGCAAG GAGCTGCGCA ACGGCTCGAC GGTGGCGCTG GTGTACCTGT CGCCGGCCGC CAAACCCGCC GCGACGGGCC TGGAGTCGAC CATGCGGTTC ATCCTGTCGG CCAAGTCCGC CTACGTCGAC GGCCAGGTCT TCTACGTCGG CGAGGCCGAC TCCACCCCCC CGGCGGACTG GGAACGGCCG CTGGACGGCA AGGTCGCCAT CGTGACCGGT GCGGCCCGCG GAATCGGCGC CACGATCGCC GAGGTGTTCG CCCGCGACGG CGCCCGCGTG GTCGCGATCG ACGTGGAATC GGCCGCCGAG ACGCTGGCCG AGACGGCCAG CCGGGTCGGC GGCACCGCGC TGTGGCTCGA CGTCACCGCC CCCGACGCCG TCGACAAGAT CACCGAGCAC CTGCGCGAGC ACCACGGCGG TCACGCCGAC ATCCTGGTCA ACAACGCCGG GATCACCCGC GACAAGCTGC TGGCCAACAT GGACGACGCG CGCTGGGACG CCGTGTTGGC CGTGAATCTG CTTGCCCCAC TTCGCCTTAC CGAAGGGCTG GTGGGCAACG GCAGCATCGG CGAAGGCGGC CGCATCGTCG GCCTTTCGTC GATGGCCGGC ATCGCGGGCA ACCGCGGCCA GACCAACTAC GCCACCACCA AGGCAGGCAT GATCGGCCTC ACCCAGGCGC TGGCGCCGGA GCTCTACGAC AAGGGCATCA CCATCAACGC CGTCGCGCCG GGATTCATCG AGACCCAGAT GACGGCCGCC ATCCCGCTGG CCACCCGCGA GGTGGGGCGC CGGATGAACT CGCTGCTGCA GGGCGGGCAG CCGGTGGACG TCGCCGAAAC CATCGCCTAC TTCGCCAGCC CGGCGTCGAA CGCGGTGACC GGCAACGTCA TCCGGGTCTG CGGCCAGGCG ATGCTGGGGG CA
Details for MypaA.00010.a.A1.GE29178
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAPKVSSD LFSQIVNSGP GSFLAKQLGV PQPETLRRYR PGDPPLAGSL LIGGEGRVVE PLRAALAKDY DLVGNNLGGR WADRFGGLVF DATGITTPEG LKGLYEFFTP LLRNLGHCAR VVVVGTTPDA AAGPHERIAQ RALEGFTRSL GKELRNGSTV ALVYLSPAAK PAATGLESTM RFILSAKSAY VDGQVFYVGE ADSTPPADWE RPLDGKVAIV TGAARGIGAT IAEVFARDGA RVVAIDVESA AETLAETASR VGGTALWLDV TAPDAVDKIT EHLREHHGGH ADILVNNAGI TRDKLLANMD DARWDAVLAV NLLAPLRLTE GLVGNGSIGE GGRIVGLSSM AGIAGNRGQT NYATTKAGMI GLTQALAPEL YDKGITINAV APGFIETQMT AAIPLATREV GRRMNSLLQG GQPVDVAETI AYFASPASNA VTGNVIRVCG QAMLGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggctccg aaggtctcgt ccgatctgtt ctcgcagatt gtcaattccg gtcctggatc gtttctcgcc aagcagctcg gcgtcccgca acccgagacg ctgcgccgct accggcccgg tgacccgccg ctggccgggt cgctgctgat cggcggcgag ggccgcgtgg tcgagccgct gcgggcggcg ctggccaagg actacgacct ggtcggcaac aacctgggcg ggcgctgggc cgaccggttc ggcgggctgg tcttcgacgc caccgggatc accaccccgg agggcctgaa ggggctgtac gagttcttca ccccactgct gcgcaacctg ggtcactgcg cccgcgtggt ggtggtcggc accacgcccg acgccgccgc cggcccgcac gagcggatcg cccagcgcgc cctggagggc ttcacccggt cattgggcaa ggagctgcgc aacggctcga cggtggcgct ggtgtacctg tcgccggccg ccaaacccgc cgcgacgggc ctggagtcga ccatgcggtt catcctgtcg gccaagtccg cctacgtcga cggccaggtc ttctacgtcg gcgaggccga ctccaccccc ccggcggact gggaacggcc gctggacggc aaggtcgcca tcgtgaccgg tgcggcccgc ggaatcggcg ccacgatcgc cgaggtgttc gcccgcgacg gcgcccgcgt ggtcgcgatc gacgtggaat cggccgccga gacgctggcc gagacggcca gccgggtcgg cggcaccgcg ctgtggctcg acgtcaccgc ccccgacgcc gtcgacaaga tcaccgagca cctgcgcgag caccacggcg gtcacgccga catcctggtc aacaacgccg ggatcacccg cgacaagctg ctggccaaca tggacgacgc gcgctgggac gccgtgttgg ccgtgaatct gcttgcccca cttcgcctta ccgaagggct ggtgggcaac ggcagcatcg gcgaaggcgg ccgcatcgtc ggcctttcgt cgatggccgg catcgcgggc aaccgcggcc agaccaacta cgccaccacc aaggcaggca tgatcggcct cacccaggcg ctggcgccgg agctctacga caagggcatc accatcaacg ccgtcgcgcc gggattcatc gagacccaga tgacggccgc catcccgctg gccacccgcg aggtggggcg ccggatgaac tcgctgctgc agggcgggca gccggtggac gtcgccgaaa ccatcgccta cttcgccagc ccggcgtcga acgcggtgac cggcaacgtc atccgggtct gcggccaggc gatgctgggg gcaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa