MythA.18628.a

Virulence factor Mce family protein (Rv0174 ortholog)

CENTER ID: MythA.18628.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.18628.a.B2.GE37659 mature protein 25 516
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.1060
RefSeq: WP_003924544.1
UniProt: G7CDL3

Orthologues for Rv0174

Species % Identity % Coverage Clones Proteins Structures
paratuberculosis 77 100 0 0 0
avium 76 60 1 0 0
thermoresistibile 72 100 1 0 0
smegmatis 69 100 1 0 0
fortuitum 67 100 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLTRFIKMQL VIFTVLTVIA LVALGWYYLR VPSLIGIGQY TLHAELPRSG GLYATANVTY RGQTIGKVTA VEPTPRGARA TMSIGSEYKI PIDSTANVHS VSAIGEQYLD LVPAGTTDVY FSDGDTITES TVPSEVGPAL DAANEGLQAL PAEKIDSLLT ETAAAVGGLG PSLQRLVDST TAIARDFRDN LEPVGDIIDN SAPILDSQVD SGDAIARWSR NVNILAAQAA QQDAALRSGL QQAAPTADQL NAVFGGVRES LPQVLANLAV VSDMLKRYHK GLNQSLVILP QGAAIAQTTT APFPGEALLH LALGINIPPP CLTGFLPPSQ WRSPADTSPQ PLPEGTYCKI PKDTQGNVVR GGRNYPCVDV PGKRAATPRE CRSDEPYVPL GTNPWYGDPN QIRNCPAPGA RCDQPVDPGK VIPAESVNNG MNPLPADRLP GPVPPVSDPL TPPGPSGMVE CSGQQPNPCI YTPAPGTAIY NPASGEAVGP DGVKYSVSNS SNPGDDGWKE MLAPAG
NT Sequence
atgctgaccc gcttcatcaa gatgcagctg gtgatcttca ccgtcctcac ggtgatcgcg ctggtggcgc tggggtggta ctacctgcgg gtgccgtcgc tgatcggcat cgggcagtac accctgcacg ccgagctgcc gcgctccggc gggctgtacg cgacggccaa cgtcacctac cggggtcaga cgatcggcaa ggtgaccgcg gtggaaccga ccccgcgcgg ggcccgggcc accatgagca tcggcagcga gtacaagatc ccgatcgact cgacggccaa cgtgcactcg gtgtcggcca tcggtgagca gtacctggat ctggtgcccg ccggcaccac ggacgtgtac ttctccgacg gtgacaccat caccgagagc acggtgccca gcgaggtcgg tccggcgctg gacgccgcga acgagggtct acaggcgctg cccgccgaga agatcgactc gttgctgacc gagacagccg ccgccgtggg tggtctgggc ccgtcgctgc aacggctggt cgactccacc accgcgatcg cccgggattt ccgggacaac ctggaaccgg tcggcgacat catcgacaac tcggcgccga tcctcgacag ccaggtcgac tccggtgacg cgatcgcgcg gtggtcgcgc aacgtcaaca tcctggccgc gcaggccgca caacaggacg cggcgctgcg cagcggtctg cagcaggccg cgccgaccgc cgatcagctg aacgcggtgt tcggcggggt gcgggagtcg ttgccgcagg tgctggcgaa cctcgcggtg gtcagcgaca tgctcaagcg ctaccacaag gggctgaacc agtcgctggt gatcctgccg cagggcgcgg cgatcgccca gaccacgacg gcgccgttcc cgggcgaggc gctgctgcac ctcgcgctgg gcatcaacat cccgccgccg tgtctgaccg ggttcctgcc gccgtcgcag tggcgctcgc cggccgacac ctcaccgcag ccgctgccgg agggtaccta ctgcaagatc ccgaaggaca cccagggcaa tgtggtgcgc ggtggccgca actatccgtg tgtggatgtg ccgggtaagc gggcggcgac gccgcgggag tgccgcagcg acgagccgta cgtgccgctg ggcaccaacc cctggtacgg ggatccgaac cagatccgca actgcccggc tcccggcgcg cgttgtgatc agcccgtcga tcccggcaag gtgatccctg cggagtcggt gaacaacggc atgaacccgt tgccggccga ccggctgccc ggtccggtcc cgccggtcag cgatccgctg accccgcctg ggccgtcggg catggtcgag tgcagtggac aacaacccaa cccctgcatc tacactcccg caccagggac ggccatctac aacccggcca gcggtgaagc ggtcggcccg gacggtgtga agtactccgt cagcaactcg agcaacccag gagacgacgg atggaaggag atgctggcac ccgccggctg a
Details for MythA.18628.a.B2.GE37659
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 52kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 85
Validated AA Sequence
MSIGSEYKIP IDSTANVHSV SAIGEQYLDL VPAGTTDVYF SDGDTITEST VPSEVGPALD AANEGLQALP AEKIDSLLTE TAAAVGGLGP SLQRLVDSTT XIARDFRDNL EPVGDIIDNS APILDSQVDS GDAIARWSRN VNILAAQAAQ QDAALRSGLQ QAAPTADQLN AVFGGVRESL PQVLANLAVV SDMLKRYHKG LNQSLVILPQ GAAIAQTTTA PFPGEALLHL ALGINIPPPC LTGFLPPSQW RSPADTSPQP LPEGTYCKIP KDTQGNVVRG GRNYPCVDVP GKRAATPREC RSDEPYVPLG TNPWYGDPNQ IRNCPAPGAR CDQPVDPGKV IPAESVNNGM NPLPADRLPG PVPPVSDPLT PPGPSGMVEC SGQQPNPCIY TPAPGTAIYN PASGEAVGPD GVKYSVSNSS NPGDDG
Validated NT Sequence
catccgtcgt ctcctgggtt gctcgagttg ctgacggagt acttcacacc gtccgggccg accgcttcac cgctggccgg gttgtagatg gccgtccctg gtgcgggagt gtagatgcag gggttgggtt gttgtccact gcactcgacc atgcccgacg gcccaggcgg ggtcagcgga tcgctgaccg gcgggaccgg accgggcagc cggtcggccg gcaacgggtt catgccgttg ttcaccgact ccgcagggat caccttgccg ggatcgacgg gctgatcaca acgcgcgccg ggagccgggc agttgcggat ctggttcgga tccccgtacc aggggttggt gcccagcggc acgtacggct cgtcgctgcg gcactcccgc ggcgtcgccg cccgcttacc cggcacatcc acacacggat agttgcggcc accgcgcacc acattgccct gggtgtcctt cgggatcttg cagtaggtac cctccggcag cggctgcggt gaggtgtcgg ccggcgagcg ccactgcgac ggcggcagga acccggtcag acacggcggc gggatgttga tgcccagcgc gaggtgcagc agcgcctcgc ccgggaacgg cgccgtcgtg gtctgggcga tcgccgcgcc ctgcggcagg atcaccagcg actggttcag ccccttgtgg tagcgcttga gcatgtcgct gaccaccgcg aggttcgcca gcacctgcgg caacgactcc cgcaccccgc cgaacaccgc gttcagctga tcggcggtcg gcgcggcctg ctgcagaccg ctgcgcagcg ccgcgtcctg ttgtgcggcc tgcgcggcca ggatgttgac gttgcgcgac caccgcgcga tcgcgtcacc ggagtcgacc tggctgtcga ggatcggcgc cgagttgtcg atgatgtcgc cgaccggttc caggttgtcc cggaaatccc gggcgatcgn ggtggtggag tcgaccagcc gttgcagnga cggncccaga ccacccacgg cggcggctgt ctcggtcagc aacgagtcga tcttctcggc gggcagcgcc tgtagaccct cgttcgcggc gtccagcgcc ggaccgacct cgctgggcac cgtgctctcg gtgatggtgt caccgtcgga gaagtacacg tccgtggtgc cggcgggcac cagatccagg tactgctcac cgatggccga caccgagtgc acgttggccg tcgagtcgat cgggatcttg tactcgctgc cgatgctcat ggtggcccgg gccccgcgcg gggtcggttc caccgcggtc accttgccga tcgtctgacc ccggtaggtg acgttggccg tcgcgtacag cccgccggag cgcggcagct cggcgtgcag ggtgtactgc ccgatgccga tcagcgacgg cacccgcagg tagtacc
Expected Protein Sequence
MAHHHHHHGW YYLRVPSLIG IGQYTLHAEL PRSGGLYATA NVTYRGQTIG KVTAVEPTPR GARATMSIGS EYKIPIDSTA NVHSVSAIGE QYLDLVPAGT TDVYFSDGDT ITESTVPSEV GPALDAANEG LQALPAEKID SLLTETAAAV GGLGPSLQRL VDSTTAIARD FRDNLEPVGD IIDNSAPILD SQVDSGDAIA RWSRNVNILA AQAAQQDAAL RSGLQQAAPT ADQLNAVFGG VRESLPQVLA NLAVVSDMLK RYHKGLNQSL VILPQGAAIA QTTTAPFPGE ALLHLALGIN IPPPCLTGFL PPSQWRSPAD TSPQPLPEGT YCKIPKDTQG NVVRGGRNYP CVDVPGKRAA TPRECRSDEP YVPLGTNPWY GDPNQIRNCP APGARCDQPV DPGKVIPAES VNNGMNPLPA DRLPGPVPPV SDPLTPPGPS GMVECSGQQP NPCIYTPAPG TAIYNPASGE AVGPDGVKYS VSNSSNPGDD GWKEMLAPAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggtggt actacctgcg ggtgccgtcg ctgatcggca tcgggcagta caccctgcac gccgagctgc cgcgctccgg cgggctgtac gcgacggcca acgtcaccta ccggggtcag acgatcggca aggtgaccgc ggtggaaccg accccgcgcg gggcccgggc caccatgagc atcggcagcg agtacaagat cccgatcgac tcgacggcca acgtgcactc ggtgtcggcc atcggtgagc agtacctgga tctggtgccc gccggcacca cggacgtgta cttctccgac ggtgacacca tcaccgagag cacggtgccc agcgaggtcg gtccggcgct ggacgccgcg aacgagggtc tacaggcgct gcccgccgag aagatcgact cgttgctgac cgagacagcc gccgccgtgg gtggtctggg cccgtcgctg caacggctgg tcgactccac caccgcgatc gcccgggatt tccgggacaa cctggaaccg gtcggcgaca tcatcgacaa ctcggcgccg atcctcgaca gccaggtcga ctccggtgac gcgatcgcgc ggtggtcgcg caacgtcaac atcctggccg cgcaggccgc acaacaggac gcggcgctgc gcagcggtct gcagcaggcc gcgccgaccg ccgatcagct gaacgcggtg ttcggcgggg tgcgggagtc gttgccgcag gtgctggcga acctcgcggt ggtcagcgac atgctcaagc gctaccacaa ggggctgaac cagtcgctgg tgatcctgcc gcagggcgcg gcgatcgccc agaccacgac ggcgccgttc ccgggcgagg cgctgctgca cctcgcgctg ggcatcaaca tcccgccgcc gtgtctgacc gggttcctgc cgccgtcgca gtggcgctcg ccggccgaca cctcaccgca gccgctgccg gagggtacct actgcaagat cccgaaggac acccagggca atgtggtgcg cggtggccgc aactatccgt gtgtggatgt gccgggtaag cgggcggcga cgccgcggga gtgccgcagc gacgagccgt acgtgccgct gggcaccaac ccctggtacg gggatccgaa ccagatccgc aactgcccgg ctcccggcgc gcgttgtgat cagcccgtcg atcccggcaa ggtgatccct gcggagtcgg tgaacaacgg catgaacccg ttgccggccg accggctgcc cggtccggtc ccgccggtca gcgatccgct gaccccgcct gggccgtcgg gcatggtcga gtgcagtgga caacaaccca acccctgcat ctacactccc gcaccaggga cggccatcta caacccggcc agcggtgaag cggtcggccc ggacggtgtg aagtactccg tcagcaactc gagcaaccca ggagacgacg gatggaagga gatgctggca cccgccggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat