MysmA.18628.a

MCE-family protein MCE1f (Rv0174 ortholog)

CENTER ID: MysmA.18628.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.18628.a.B2.GE37633 mature protein 26 518
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.136
RefSeq: YP_006564912.1
RefSeq: YP_884555.1
UniProt: A0QNR7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLTRFIKMQ LVIFLTLTLV ALVVLALFYL RLPTWAGLGM YKLNADLPNS GGLYATANVT YRGTTIGKVT SVEPSESGAR VEMNIYDRYK IPADATANVH SVSAVGEQFI DLTSDSGGGA YFQPGDTITK ATVPAEVGPA LDAAEKGLAV LPKEKIGTLL DEAATAFGGL GPSLQRLVDS TQAIAGDFRA NIDPVNDIIE NSGPIIDSQV NSGDAIQRWA ANLNTLAAQS AQNDEALRSG LQQAAPTADQ LNAVFSDVRE SLPQTLANLE IVIDMLKRYN KNVEQVLVAL PQGAAVAQTG TIFAPEGLLH FGLGINAPPP CLTGFLPASQ WRSPADTRTE PLPSGLYCKI PKDAPNAVRG ARNYPCADVP GKRAATPREC RSDEPYQPLG TNPWYGDPDQ IRNCPAPGAR CDQPVDPGRV IPAPSINNGL NPLPASQLPP PEVSSGPSSD PLTAPRGGTV TCSGQQPNPC IYTPAAGATA TYNPASGEVV GPGGVKYSVT NSNTPGDDGW KEMLAPAS
NT Sequence
atgctgctga ctcgcttcat caagatgcag ctcgtcatct tcctcacgct gacgctggtg gcgctggtgg tgctcgcgct gttctacctg cgcctgccga catgggccgg gctcggcatg tacaagctca atgccgacct gcccaactcg ggcggcctgt acgccaccgc caacgtcacg taccgcggca ccacgatcgg caaggtgacc tcggtggagc ccagcgagtc gggcgcgcgg gtcgagatga acatctacga ccggtacaag atcccggccg acgcgacggc caacgtgcac tcggtgtcgg ccgtcggtga gcagttcatc gacctcacct ccgactccgg cggcggtgcc tacttccaac ccggcgacac catcaccaag gcgacggtgc ccgccgaggt gggtcctgcg ctcgacgccg ccgagaaggg tctggcggtg ctgccgaagg agaagatcgg cacgctgctc gacgaggcgg ccacggcgtt cggtggcctc gggccgtcac tgcagcgcct cgtcgattcg acgcaggcca tcgccggtga cttccgggcc aacatcgacc cggtcaacga catcatcgag aactccgggc cgatcatcga cagccaggtg aattcgggcg acgcgatcca gcggtgggcg gccaacctca acacgctggc cgcgcagagc gcccagaacg acgaggcgct gcgcagcggc ctgcagcagg cggccccgac ggccgatcag ctcaacgcgg tgttcagcga cgtccgcgag tcgttgccac agacgctggc gaacctcgag atcgtcatcg acatgctcaa gcgctacaac aagaacgtcg agcaggtcct ggtggcgctg ccccagggcg ccgcggtcgc ccagaccggc acgatcttcg cgccagaggg tctgctgcac ttcggtctcg gcatcaacgc gccgccgccg tgcctgacgg gcttcctgcc cgcctcgcag tggcgctcgc cggccgatac gcgcaccgaa ccgctgccgt cgggcctgta ctgcaagatc ccaaaggacg cgcccaacgc ggtccgcggt gcccgtaact atccctgcgc cgacgtgccg ggcaagcggg ccgccacacc gcgggaatgc cgtagcgacg agccctacca gccgctgggc accaacccct ggtacggcga tccggaccag atccgcaact gcccggctcc cggggcccgc tgcgatcagc cggtcgatcc gggacgtgtg atcccggcgc cgtcgatcaa caacgggctc aacccgttgc ccgcgagcca gctgccgccg cccgaggtga gttcgggccc gagcagcgat ccgttgaccg caccgcgcgg tggcacggtg acgtgtagtg gacagcaacc caacccttgc atctacactc cggctgcagg cgcgaccgcg acctacaacc cggccagcgg tgaggtggtc ggcccaggcg gtgtgaagta ctccgtcacc aactcgaata ccccaggaga tgacggatgg aaggagatgc tggcgccagc cagctga
Details for MysmA.18628.a.B2.GE37633
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 52kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 78
Validated AA Sequence
MAHHHHHHMA LFYLRLPTWA GLGMYKLNAD LPNSGGLYAT ANVTYRGTTI GKVTSVEPSE SGARVEMNIY DRYKIPADAT ANVHSVSAVG EQFIDLTSDS GGGAYFQPGD TITKATVPAE VGPALDAAEK GLAVLPKEKI GTLLDEAATA FGGLGPSLQR LVDSTQAIAG DFRANIDPVN DIIENSGPII DSQVNSGDAI QRWAANLNTL AAQSAQNDEA LRSGLQQAAP TADQLNAVFS DVRESLPQTL ANLEIVIDML KRYNKNVEQV LVALPQGAAV AQTGTIFAPE GLLHFGLGIN APPPCLTGFL PASQWRSPAD TRTETAAVGP VLQIPKDAPX RSRARTIPAX TCRAAGRXHR XXRXXXPXXA XXXXXGXXXX XXXRXXXXRX ESGXX
Validated NT Sequence
tananntttt gtttaacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atggcgctgt tctacctgcg cctgccgaca tgggccgggc tcggcatgta caagctcaat gccgacctgc ccaactcggg cggcctgtac gccaccgcca acgtcacgta ccgcggcacc acgatcggca aggtgacctc ggtggagccc agcgagtcgg gcgcgcgggt cgagatgaac atctacgacc ggtacaagat cccggccgac gcgacggcca acgtgcactc ggtgtcggcc gtcggtgagc agttcatcga cctcacctcc gactccggcg gcggtgccta cttccaaccc ggcgacacca tcaccaaggc gacggtgccc gccgaggtgg gtcctgcgct cgacgccgcc gagaagggtc tggcggtgct gccgaaggag aagatcggca cgctgctcga cgaggcggcc acggcgttcg gtggcctcgg gccgtcactg cagcgcctcg tcgattcgac gcaggccatc gccggtgact tccgggccaa catcgacccg gtcaacgaca tcatcgagaa ctccgggccg atcatcgaca gccaggtgaa ttcgggcgac gcgatccagc ggtgggcggc caacctcaac acgctggccg cgcagagcgc ccagaacgac gaggcgctgc gcagcggcct gcagcaggcg gccccgacgg ccgatcagct caacgcggtg ttcagcgacg tccgcgagtc gttgccacag acgctggcga acctcgagat cgtcatcgac atgctcaagc gctacaacaa gaacgtcgag caggtcctgg tggcgctgcc ccagggcgcc gcggtcgccc agaccggcac gatcttcgcg ccagagggtc tgctgcactt cggtctcggc atcaacgcgc cgccgccgtg cctgacgggc ttcctgcccg cctcgcagtg gcgctcgccg gccgatacgc gcaccgaaac cgctgccgtc gggcctgtac tgcagatccc aaaggacgcg cccnngcggt cncgngcccg tactatccct gcgncgacgt gccgggcagc gggccgccna caccgcngga ngcgtancna cnanccctnc nncgctgnnn ncnnccnnnn cggnnatnnn ncnatnnnnc ngnnccgnnn nnncnatcan cggntcgaat ccggnannnt gtgatnnnc
Expected Protein Sequence
MAHHHHHHAL FYLRLPTWAG LGMYKLNADL PNSGGLYATA NVTYRGTTIG KVTSVEPSES GARVEMNIYD RYKIPADATA NVHSVSAVGE QFIDLTSDSG GGAYFQPGDT ITKATVPAEV GPALDAAEKG LAVLPKEKIG TLLDEAATAF GGLGPSLQRL VDSTQAIAGD FRANIDPVND IIENSGPIID SQVNSGDAIQ RWAANLNTLA AQSAQNDEAL RSGLQQAAPT ADQLNAVFSD VRESLPQTLA NLEIVIDMLK RYNKNVEQVL VALPQGAAVA QTGTIFAPEG LLHFGLGINA PPPCLTGFLP ASQWRSPADT RTEPLPSGLY CKIPKDAPNA VRGARNYPCA DVPGKRAATP RECRSDEPYQ PLGTNPWYGD PDQIRNCPAP GARCDQPVDP GRVIPAPSIN NGLNPLPASQ LPPPEVSSGP SSDPLTAPRG GTVTCSGQQP NPCIYTPAAG ATATYNPASG EVVGPGGVKY SVTNSNTPGD DGWKEMLAPA S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgctgt tctacctgcg cctgccgaca tgggccgggc tcggcatgta caagctcaat gccgacctgc ccaactcggg cggcctgtac gccaccgcca acgtcacgta ccgcggcacc acgatcggca aggtgacctc ggtggagccc agcgagtcgg gcgcgcgggt cgagatgaac atctacgacc ggtacaagat cccggccgac gcgacggcca acgtgcactc ggtgtcggcc gtcggtgagc agttcatcga cctcacctcc gactccggcg gcggtgccta cttccaaccc ggcgacacca tcaccaaggc gacggtgccc gccgaggtgg gtcctgcgct cgacgccgcc gagaagggtc tggcggtgct gccgaaggag aagatcggca cgctgctcga cgaggcggcc acggcgttcg gtggcctcgg gccgtcactg cagcgcctcg tcgattcgac gcaggccatc gccggtgact tccgggccaa catcgacccg gtcaacgaca tcatcgagaa ctccgggccg atcatcgaca gccaggtgaa ttcgggcgac gcgatccagc ggtgggcggc caacctcaac acgctggccg cgcagagcgc ccagaacgac gaggcgctgc gcagcggcct gcagcaggcg gccccgacgg ccgatcagct caacgcggtg ttcagcgacg tccgcgagtc gttgccacag acgctggcga acctcgagat cgtcatcgac atgctcaagc gctacaacaa gaacgtcgag caggtcctgg tggcgctgcc ccagggcgcc gcggtcgccc agaccggcac gatcttcgcg ccagagggtc tgctgcactt cggtctcggc atcaacgcgc cgccgccgtg cctgacgggc ttcctgcccg cctcgcagtg gcgctcgccg gccgatacgc gcaccgaacc gctgccgtcg ggcctgtact gcaagatccc aaaggacgcg cccaacgcgg tccgcggtgc ccgtaactat ccctgcgccg acgtgccggg caagcgggcc gccacaccgc gggaatgccg tagcgacgag ccctaccagc cgctgggcac caacccctgg tacggcgatc cggaccagat ccgcaactgc ccggctcccg gggcccgctg cgatcagccg gtcgatccgg gacgtgtgat cccggcgccg tcgatcaaca acgggctcaa cccgttgccc gcgagccagc tgccgccgcc cgaggtgagt tcgggcccga gcagcgatcc gttgaccgca ccgcgcggtg gcacggtgac gtgtagtgga cagcaaccca acccttgcat ctacactccg gctgcaggcg cgaccgcgac ctacaacccg gccagcggtg aggtggtcgg cccaggcggt gtgaagtact ccgtcaccaa ctcgaatacc ccaggagatg acggatggaa ggagatgctg gcgccagcca gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat