MythA.01365.a
Short chain dehydrogenase (A0QTM2 homolog)
| CENTER ID: | MythA.01365.a |
| ORGANISM: | Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409 |
| ASSOCIATED DISEASE: | Respiratory Infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.01365.a.A1.GE28786 | Full length( MythA.01365.a ) | 1 | 290 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.01365.a.A1.PW28688 | Full length( MythA.01365.a ) | 1 | 290 |
Structures
3SC4
DEPOSITED: 6/6/2011
DETERMINATION: XRay
CLONE: MythA.01365.a.A1.GE28786
PROTEIN: MythA.01365.a.A1.PW28688
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130649 |
| PATRIC ID: | fig|1078020.3.peg.4446 |
| RefSeq: | WP_003927957.1 |
| UniProt: | G7CNA1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLRGKTMFI SGGSRGIGPX XXXXXXXXXX XXXXXXXXXX XXXXXXXXPN CPAPSTRPPR RSRRPEVRRC PSSATSATAT RAPRXXXXXX XXXXQFGGID ICVNNASAIN LGSIEEVPLK RFDLMNGIQV RGTYAVSQSC IPHMKGRDNP HILTLSPPIR LEPKWLRPTP YMMAKYGMTL CALGIAEELR DAGIASNTLW PRTTVATAAV QNLLGGDEAM ARSRKPEVYA DAAYVVLNKP SSYTGNTLLC EDVLLESGVT DLSVYDCVPG SELGVDLWVD SPNPPGYTGP
NT Sequence
ATGTCGCTGC GCGGAAAAAC CATGTTCATC TCCGGTGGCA GCCGCGGGAT CGGCCCCNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCCAAAC TGCCCGGCAC CATCTACACG GCCGCCAAGG AGATCGAGGA GGCCGGAGGT CAGGCGTTGC CCATCGTCGG CGACATCCGC GACGGCGACG CGGGCGCCCC GGCNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCAGTTCGG CGGCATCGAC ATCTGCGTCA ACAACGCCTC GGCGATCAAC CTCGGCTCCA TCGAAGAGGT CCCGTTGAAG CGGTTCGACC TGATGAACGG GATCCAGGTG CGTGGCACGT ACGCGGTGTC GCAGTCCTGC ATCCCGCACA TGAAGGGCCG CGACAACCCG CACATCCTGA CGCTCTCACC ACCGATCCGG CTGGAACCCA AATGGTTGCG GCCGACGCCG TACATGATGG CCAAGTACGG GATGACATTG TGCGCGTTGG GAATCGCAGA GGAACTGCGG GACGCCGGAA TCGCCTCGAA CACGCTGTGG CCGCGGACCA CGGTGGCGAC GGCCGCGGTG CAGAACCTGC TCGGCGGCGA TGAGGCGATG GCCCGGTCGC GCAAACCGGA GGTGTACGCC GACGCCGCCT ATGTGGTGCT GAACAAACCG TCCAGCTACA CCGGCAACAC CCTGTTGTGT GAGGACGTCC TGCTGGAGTC CGGTGTCACC GACCTGTCGG TGTATGACTG CGTCCCGGGT TCCGAACTCG GGGTGGATCT GTGGGTGGAC AGCCCCAATC CACCGGGATA CACCGGCCCG
Details for MythA.01365.a.A1.GE28786
| HARVESTED ON: | 3/1/2010 |
| SEQUENCED ON: | 3/5/2010 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 79 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLRGKTMF ISGGSRGIGL AIAKRVAADG ANVALVAKSA EPHPKLPGTI YTAAKEIEEA GGQALPIVGD IRDGDAVAAA VAKTVEQFGG IDICVNNASA INLGSIEEVP LKRFDLMNGI QVRGTYAVSQ SCIPHMKGRD NPHILTLSPP IRLEPKWLRP TPYMMAKYGM TLCALGIAEE LRDAGIASNT LWPRTTVATA AVQNLLGGDE AMARSRKPEV YADAAYVVLN KPSSYTGNTL LCEDVLLESG VTDLSVYDCV PGSELGVDLW VDSPNPPGYT GP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcgc tgcgcggaaa aaccatgttc atctccggtg gcagccgcgg gatcggcctc gcgatcgcca agcgggtggc cgccgacggc gccaacgtcg cgctggtggc caagtccgcc gaaccgcatc ccaaactgcc cggcaccatc tacacggccg ccaaggagat cgaggaggcc ggaggtcagg cgttgcccat cgtcggcgac atccgcgacg gcgacgcggt cgccgcggcg gtggccaaga ccgtcgaaca gttcggcggc atcgacatct gcgtcaacaa cgcctcggcg atcaacctcg gctccatcga agaggtcccg ttgaagcggt tcgacctgat gaacgggatc caggtgcgtg gcacgtacgc ggtgtcgcag tcctgcatcc cgcacatgaa gggccgcgac aacccgcaca tcctgacgct ctcaccaccg atccggctgg aacccaaatg gttgcggccg acgccgtaca tgatggccaa gtacgggatg acattgtgcg cgttgggaat cgcagaggaa ctgcgggacg ccggaatcgc ctcgaacacg ctgtggccgc ggaccacggt ggcgacggcc gcggtgcaga acctgctcgg cggcgatgag gcgatggccc ggtcgcgcaa accggaggtg tacgccgacg ccgcctatgt ggtgctgaac aaaccgtcca gctacaccgg caacaccctg ttgtgtgagg acgtcctgct ggagtccggt gtcaccgacc tgtcggtgta tgactgcgtc ccgggttccg aactcggggt ggatctgtgg gtggacagcc ccaatccacc gggatacacc ggcccgtaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLRGKTMF ISGGSRGIGP XXXXXXXXXX XXXXXXXXXX XXXXXXXXXP NCPAPSTRPP RRSRRPEVRR CPSSATSATA TRAPRXXXXX XXXXXQFGGI DICVNNASAI NLGSIEEVPL KRFDLMNGIQ VRGTYAVSQS CIPHMKGRDN PHILTLSPPI RLEPKWLRPT PYMMAKYGMT LCALGIAEEL RDAGIASNTL WPRTTVATAA VQNLLGGDEA MARSRKPEVY ADAAYVVLNK PSSYTGNTLL CEDVLLESGV TDLSVYDCVP GSELGVDLWV DSPNPPGYTG P
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgctgcgc ggaaaaacca tgttcatctc cggtggcagc cgcgggatcg gccccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccaaactg cccggcacca tctacacggc cgccaaggag atcgaggagg ccggaggtca ggcgttgccc atcgtcggcg acatccgcga cggcgacgcg ggcgccccgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cagttcggcg gcatcgacat ctgcgtcaac aacgcctcgg cgatcaacct cggctccatc gaagaggtcc cgttgaagcg gttcgacctg atgaacggga tccaggtgcg tggcacgtac gcggtgtcgc agtcctgcat cccgcacatg aagggccgcg acaacccgca catcctgacg ctctcaccac cgatccggct ggaacccaaa tggttgcggc cgacgccgta catgatggcc aagtacggga tgacattgtg cgcgttggga atcgcagagg aactgcggga cgccggaatc gcctcgaaca cgctgtggcc gcggaccacg gtggcgacgg ccgcggtgca gaacctgctc ggcggcgatg aggcgatggc ccggtcgcgc aaaccggagg tgtacgccga cgccgcctat gtggtgctga acaaaccgtc cagctacacc ggcaacaccc tgttgtgtga ggacgtcctg ctggagtccg gtgtcaccga cctgtcggtg tatgactgcg tcccgggttc cgaactcggg gtggatctgt gggtggacag ccccaatcca ccgggataca ccggcccgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MythA.01365.a.A1.PW28688
| PURIFICATION DATe: | 6/28/2010 |
| CONCENTRATION: | 27.82mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 79 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLRGKTMF ISGGSRGIGL AIAKRVAADG ANVALVAKSA EPHPKLPGTI YTAAKEIEEA GGQALPIVGD IRDGDAVAAA VAKTVEQFGG IDICVNNASA INLGSIEEVP LKRFDLMNGI QVRGTYAVSQ SCIPHMKGRD NPHILTLSPP IRLEPKWLRP TPYMMAKYGM TLCALGIAEE LRDAGIASNT LWPRTTVATA AVQNLLGGDE AMARSRKPEV YADAAYVVLN KPSSYTGNTL LCEDVLLESG VTDLSVYDCV PGSELGVDLW VDSPNPPGYT GP
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcgc tgcgcggaaa aaccatgttc atctccggtg gcagccgcgg gatcggcctc gcgatcgcca agcgggtggc cgccgacggc gccaacgtcg cgctggtggc caagtccgcc gaaccgcatc ccaaactgcc cggcaccatc tacacggccg ccaaggagat cgaggaggcc ggaggtcagg cgttgcccat cgtcggcgac atccgcgacg gcgacgcggt cgccgcggcg gtggccaaga ccgtcgaaca gttcggcggc atcgacatct gcgtcaacaa cgcctcggcg atcaacctcg gctccatcga agaggtcccg ttgaagcggt tcgacctgat gaacgggatc caggtgcgtg gcacgtacgc ggtgtcgcag tcctgcatcc cgcacatgaa gggccgcgac aacccgcaca tcctgacgct ctcaccaccg atccggctgg aacccaaatg gttgcggccg acgccgtaca tgatggccaa gtacgggatg acattgtgcg cgttgggaat cgcagaggaa ctgcgggacg ccggaatcgc ctcgaacacg ctgtggccgc ggaccacggt ggcgacggcc gcggtgcaga acctgctcgg cggcgatgag gcgatggccc ggtcgcgcaa accggaggtg tacgccgacg ccgcctatgt ggtgctgaac aaaccgtcca gctacaccgg caacaccctg ttgtgtgagg acgtcctgct ggagtccggt gtcaccgacc tgtcggtgta tgactgcgtc ccgggttccg aactcggggt ggatctgtgg gtggacagcc ccaatccacc gggatacacc ggcccgtaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLRGKTMF ISGGSRGIGP XXXXXXXXXX XXXXXXXXXX XXXXXXXXXP NCPAPSTRPP RRSRRPEVRR CPSSATSATA TRAPRXXXXX XXXXXQFGGI DICVNNASAI NLGSIEEVPL KRFDLMNGIQ VRGTYAVSQS CIPHMKGRDN PHILTLSPPI RLEPKWLRPT PYMMAKYGMT LCALGIAEEL RDAGIASNTL WPRTTVATAA VQNLLGGDEA MARSRKPEVY ADAAYVVLNK PSSYTGNTLL CEDVLLESGV TDLSVYDCVP GSELGVDLWV DSPNPPGYTG P
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGCTGCGC GGAAAAACCA TGTTCATCTC CGGTGGCAGC CGCGGGATCG GCCCCNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCAAACTG CCCGGCACCA TCTACACGGC CGCCAAGGAG ATCGAGGAGG CCGGAGGTCA GGCGTTGCCC ATCGTCGGCG ACATCCGCGA CGGCGACGCG GGCGCCCCGG CNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CAGTTCGGCG GCATCGACAT CTGCGTCAAC AACGCCTCGG CGATCAACCT CGGCTCCATC GAAGAGGTCC CGTTGAAGCG GTTCGACCTG ATGAACGGGA TCCAGGTGCG TGGCACGTAC GCGGTGTCGC AGTCCTGCAT CCCGCACATG AAGGGCCGCG ACAACCCGCA CATCCTGACG CTCTCACCAC CGATCCGGCT GGAACCCAAA TGGTTGCGGC CGACGCCGTA CATGATGGCC AAGTACGGGA TGACATTGTG CGCGTTGGGA ATCGCAGAGG AACTGCGGGA CGCCGGAATC GCCTCGAACA CGCTGTGGCC GCGGACCACG GTGGCGACGG CCGCGGTGCA GAACCTGCTC GGCGGCGATG AGGCGATGGC CCGGTCGCGC AAACCGGAGG TGTACGCCGA CGCCGCCTAT GTGGTGCTGA ACAAACCGTC CAGCTACACC GGCAACACCC TGTTGTGTGA GGACGTCCTG CTGGAGTCCG GTGTCACCGA CCTGTCGGTG TATGACTGCG TCCCGGGTTC CGAACTCGGG GTGGATCTGT GGGTGGACAG CCCCAATCCA CCGGGATACA CCGGCCCGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA