MyulA.00472.a

Iron-regulated short-chain dehydrogenase/reductase

CENTER ID: MyulA.00472.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00472.a.A1.GE29699 Full length( MyulA.00472.a ) 1 282
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00472.a.A1.PS00780a Full length( MyulA.00472.a ) 1 282

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.2986
RefSeq: YP_906360.1
UniProt: A0PRC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLNGKTMFI SGASRGIGLA IAKRAAQDGA NIALIAKTAE PHPKLPGTVY TAAKELEEAG GQALPIVGDV RDPDSVSAAV AKTVEQFGGI DICVNNASAI NLGSITEVPM KRFDLMNGIQ VRGTYAVSQA CIPHLKGREN PHILTLSPPV QLDKKWLKPT AYMMAKFGMT LCALGIAEEM RDEGIASNTL WPRTLVATAA VQNLLGGEEA MGRARKPDVY ADAAYVILNK NSREYTGNSA LCEDVLLESG VSDLSIYNCV PGAKLGVDLW VEEVNPPGYT QP
NT Sequence
ATGTCCCTTA ACGGCAAGAC CATGTTCATC TCCGGCGCCA GCCGCGGTAT CGGCCTGGCG ATCGCCAAGC GGGCCGCACA AGACGGTGCG AACATCGCAC TGATCGCCAA GACCGCCGAA CCCCACCCGA AGCTTCCGGG CACCGTATAC ACGGCAGCCA AAGAGCTCGA GGAAGCCGGC GGTCAGGCAC TGCCGATCGT CGGGGACGTC CGCGACCCGG ACTCCGTCAG CGCCGCCGTT GCCAAGACGG TGGAACAGTT CGGCGGCATC GACATCTGTG TCAACAATGC CTCCGCCATC AACCTCGGGT CCATCACCGA AGTGCCGATG AAGCGGTTCG ACCTGATGAA CGGCATCCAG GTGCGCGGCA CCTATGCGGT GTCGCAGGCG TGCATCCCGC ACCTGAAGGG CCGGGAAAAC CCGCACATCT TGACGCTTTC TCCGCCAGTG CAGCTGGACA AGAAGTGGCT GAAGCCGACC GCATACATGA TGGCCAAGTT CGGTATGACG CTGTGCGCGT TGGGAATCGC CGAGGAGATG CGCGACGAGG GCATCGCCTC GAACACGCTG TGGCCCCGCA CCCTGGTGGC AACCGCCGCG GTGCAGAACC TGCTCGGCGG CGAGGAGGCG ATGGGACGGG CCCGCAAACC CGATGTCTAT GCCGATGCGG CCTACGTCAT CCTCAACAAG AACTCCCGCG AGTACACCGG CAACTCGGCG TTGTGCGAGG ACGTGCTGCT CGAGTCGGGA GTGAGCGACC TTTCGATCTA CAACTGCGTG CCCGGGGCCA AGCTGGGCGT GGACCTGTGG GTGGAAGAGG TCAACCCGCC GGGGTACACG CAGCCC
Details for MyulA.00472.a.A1.GE29699
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLNGKTMF ISGASRGIGL AIAKRAAQDG ANIALIAKTA EPHPKLPGTV YTAAKELEEA GGQALPIVGD VRDPDSVSAA VAKTVEQFGG IDICVNNASA INLGSITEVP MKRFDLMNGI QVRGTYAVSQ ACIPHLKGRE NPHILTLSPP VQLDKKWLKP TAYMMAKFGM TLCALGIAEE MRDEGIASNT LWPRTLVATA AVQNLLGGEE AMGRARKPDV YADAAYVILN KNSREYTGNS ALCEDVLLES GVSDLSIYNC VPGAKLGVDL WVEEVNPPGY
Validated NT Sequence
cgtgtacccc ggcgggttga cctcttccac ccacaggtcc acgcccagct tggccccggg cacgcagttg tagatcgaaa ggtcgctcac tcccgactcg agcagcacgt cctcgcacaa cgccgagttg ccggtgtact cgcgggagtt cttgttgagg atgacgtagg ccgcatcggc atagacatcg ggtttgcggg cccgtcccat cgcctcctcg ccgccgagca ggttctgcac cgcggcggtt gccaccaggg tgcggggcca cagcgtgttc gaggcgatgc cctcgtcgcg catctcctcg gcgattccca acgcgcacag cgtcataccg aacttggcca tcatgtatgc ggtcggcttc agccacttct tgtccagctg cactggcgga gaaagcgtca agatgtgcgg gttttcccgg cccttcaggt gcgggatgca cgcctgcgac accgcatagg tgccgcgcac ctggatgccg ttcatcaggt cgaaccgctt catcggcact tcggtgatgg acccgaggtt gatggcggag gcattgttga cacagatgtc gatgccgccg aactgttcca ccgtcttggc aacggcggcg ctgacggagt ccgggtcgcg gacgtccccg acgatcggca gtgcctgacc gccggcttcc tcgagctctt tggctgccgt gtatacggtg cccggaagct tcgggtgggg ttcggcggtc ttggcgatca gtgcgatgtt cgcaccgtct tgtgcggccc gcttggcgat cgccaggccg ataccgcggc tggcgccgga gatgaacatg gtcttgccgt taagggacat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLNGKTMF ISGASRGIGL AIAKRAAQDG ANIALIAKTA EPHPKLPGTV YTAAKELEEA GGQALPIVGD VRDPDSVSAA VAKTVEQFGG IDICVNNASA INLGSITEVP MKRFDLMNGI QVRGTYAVSQ ACIPHLKGRE NPHILTLSPP VQLDKKWLKP TAYMMAKFGM TLCALGIAEE MRDEGIASNT LWPRTLVATA AVQNLLGGEE AMGRARKPDV YADAAYVILN KNSREYTGNS ALCEDVLLES GVSDLSIYNC VPGAKLGVDL WVEEVNPPGY TQP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcccttaac ggcaagacca tgttcatctc cggcgccagc cgcggtatcg gcctggcgat cgccaagcgg gccgcacaag acggtgcgaa catcgcactg atcgccaaga ccgccgaacc ccacccgaag cttccgggca ccgtatacac ggcagccaaa gagctcgagg aagccggcgg tcaggcactg ccgatcgtcg gggacgtccg cgacccggac tccgtcagcg ccgccgttgc caagacggtg gaacagttcg gcggcatcga catctgtgtc aacaatgcct ccgccatcaa cctcgggtcc atcaccgaag tgccgatgaa gcggttcgac ctgatgaacg gcatccaggt gcgcggcacc tatgcggtgt cgcaggcgtg catcccgcac ctgaagggcc gggaaaaccc gcacatcttg acgctttctc cgccagtgca gctggacaag aagtggctga agccgaccgc atacatgatg gccaagttcg gtatgacgct gtgcgcgttg ggaatcgccg aggagatgcg cgacgagggc atcgcctcga acacgctgtg gccccgcacc ctggtggcaa ccgccgcggt gcagaacctg ctcggcggcg aggaggcgat gggacgggcc cgcaaacccg atgtctatgc cgatgcggcc tacgtcatcc tcaacaagaa ctcccgcgag tacaccggca actcggcgtt gtgcgaggac gtgctgctcg agtcgggagt gagcgacctt tcgatctaca actgcgtgcc cggggccaag ctgggcgtgg acctgtgggt ggaagaggtc aacccgccgg ggtacacgca gcccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MyulA.00472.a.A1.PS00780a
PURIFICATION DATe: 10/7/2010
CONCENTRATION: 15.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLNGKTMF ISGASRGIGL AIAKRAAQDG ANIALIAKTA EPHPKLPGTV YTAAKELEEA GGQALPIVGD VRDPDSVSAA VAKTVEQFGG IDICVNNASA INLGSITEVP MKRFDLMNGI QVRGTYAVSQ ACIPHLKGRE NPHILTLSPP VQLDKKWLKP TAYMMAKFGM TLCALGIAEE MRDEGIASNT LWPRTLVATA AVQNLLGGEE AMGRARKPDV YADAAYVILN KNSREYTGNS ALCEDVLLES GVSDLSIYNC VPGAKLGVDL WVEEVNPPGY
Validated NT Sequence
cgtgtacccc ggcgggttga cctcttccac ccacaggtcc acgcccagct tggccccggg cacgcagttg tagatcgaaa ggtcgctcac tcccgactcg agcagcacgt cctcgcacaa cgccgagttg ccggtgtact cgcgggagtt cttgttgagg atgacgtagg ccgcatcggc atagacatcg ggtttgcggg cccgtcccat cgcctcctcg ccgccgagca ggttctgcac cgcggcggtt gccaccaggg tgcggggcca cagcgtgttc gaggcgatgc cctcgtcgcg catctcctcg gcgattccca acgcgcacag cgtcataccg aacttggcca tcatgtatgc ggtcggcttc agccacttct tgtccagctg cactggcgga gaaagcgtca agatgtgcgg gttttcccgg cccttcaggt gcgggatgca cgcctgcgac accgcatagg tgccgcgcac ctggatgccg ttcatcaggt cgaaccgctt catcggcact tcggtgatgg acccgaggtt gatggcggag gcattgttga cacagatgtc gatgccgccg aactgttcca ccgtcttggc aacggcggcg ctgacggagt ccgggtcgcg gacgtccccg acgatcggca gtgcctgacc gccggcttcc tcgagctctt tggctgccgt gtatacggtg cccggaagct tcgggtgggg ttcggcggtc ttggcgatca gtgcgatgtt cgcaccgtct tgtgcggccc gcttggcgat cgccaggccg ataccgcggc tggcgccgga gatgaacatg gtcttgccgt taagggacat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLNGKTMF ISGASRGIGL AIAKRAAQDG ANIALIAKTA EPHPKLPGTV YTAAKELEEA GGQALPIVGD VRDPDSVSAA VAKTVEQFGG IDICVNNASA INLGSITEVP MKRFDLMNGI QVRGTYAVSQ ACIPHLKGRE NPHILTLSPP VQLDKKWLKP TAYMMAKFGM TLCALGIAEE MRDEGIASNT LWPRTLVATA AVQNLLGGEE AMGRARKPDV YADAAYVILN KNSREYTGNS ALCEDVLLES GVSDLSIYNC VPGAKLGVDL WVEEVNPPGY TQP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCCTTAAC GGCAAGACCA TGTTCATCTC CGGCGCCAGC CGCGGTATCG GCCTGGCGAT CGCCAAGCGG GCCGCACAAG ACGGTGCGAA CATCGCACTG ATCGCCAAGA CCGCCGAACC CCACCCGAAG CTTCCGGGCA CCGTATACAC GGCAGCCAAA GAGCTCGAGG AAGCCGGCGG TCAGGCACTG CCGATCGTCG GGGACGTCCG CGACCCGGAC TCCGTCAGCG CCGCCGTTGC CAAGACGGTG GAACAGTTCG GCGGCATCGA CATCTGTGTC AACAATGCCT CCGCCATCAA CCTCGGGTCC ATCACCGAAG TGCCGATGAA GCGGTTCGAC CTGATGAACG GCATCCAGGT GCGCGGCACC TATGCGGTGT CGCAGGCGTG CATCCCGCAC CTGAAGGGCC GGGAAAACCC GCACATCTTG ACGCTTTCTC CGCCAGTGCA GCTGGACAAG AAGTGGCTGA AGCCGACCGC ATACATGATG GCCAAGTTCG GTATGACGCT GTGCGCGTTG GGAATCGCCG AGGAGATGCG CGACGAGGGC ATCGCCTCGA ACACGCTGTG GCCCCGCACC CTGGTGGCAA CCGCCGCGGT GCAGAACCTG CTCGGCGGCG AGGAGGCGAT GGGACGGGCC CGCAAACCCG ATGTCTATGC CGATGCGGCC TACGTCATCC TCAACAAGAA CTCCCGCGAG TACACCGGCA ACTCGGCGTT GTGCGAGGAC GTGCTGCTCG AGTCGGGAGT GAGCGACCTT TCGATCTACA ACTGCGTGCC CGGGGCCAAG CTGGGCGTGG ACCTGTGGGT GGAAGAGGTC AACCCGCCGG GGTACACGCA GCCCAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA