SSGCID: Target Page

MythA.01263.c

Isovaleryl-CoA dehydrogenase (A0QTW7 homolog)

CENTER ID:	MythA.01263.c
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01263.c.A1.GE28776	Full length( MythA.01263.c )		1	386

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01263.c.A1.PS00646	Full length( MythA.01263.c )		1	386

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.1006
RefSeq:	WP_003924491.1
UniProt:	G7CDG1

Orthologues for Rv0215c

Species	% Identity	% Coverage	Clones	Proteins	Structures
thermoresistibile	85	100	1	1	0
smegmatis	72	99	0	2	1
thermoresistibile	32	98	0	0	0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNVVLNDEET MLVETVRAFI DRDVKPTVRE VEHANEYPQA WIEQMKRIGI YGLAIPESYG GSPVSMPCYV EVTQELSRGW MSLAGAMGGH TVVAKLLTLF GTEEQKQRYL PAMATGEIRA TMALTEPGGG SDLQNMTTTA LPGPGGGLVI NGAKTWISNA RRSGLIALLC KTDPDATPRH RGISVVLVEQ GTTGLSVSRD LPKLGYKGVE SCELVFDDCR VPASAILGGE PGKGFTQMMK GLETGRIQVA ARALGVATAA LEDXXXYAQQ RESFGQPIWK HQAIGHYLAD MATKLTAARQ LTRYAAERYD SGQRCDMEAG MAKLFASEVA MEIALNAVRI HGGYGYSTEF DVERYFRDAP LMIVGEGTNE IQRNVIVSQL VARNTV

NT Sequence

GTGAATGTCG TCCTCAACGA TGAAGAGACC ATGCTGGTCG AGACGGTGCG GGCGTTCATC GACCGCGACG TCAAGCCCAC CGTGCGCGAG GTCGAACACG CCAACGAATA TCCGCAGGCG TGGATCGAGC AGATGAAACG CATCGGCATC TACGGGCTGG CGATCCCGGA GTCCTACGGC GGCTCACCGG TGTCGATGCC GTGTTATGTC GAGGTGACCC AGGAGCTGTC GCGCGGCTGG ATGAGCCTGG CCGGGGCGAT GGGCGGGCAC ACCGTGGTGG CCAAACTGTT GACGCTGTTC GGCACCGAGG AACAGAAACA GCGCTACCTG CCGGCGATGG CCACCGGCGA GATCCGCGCG ACCATGGCGC TCACCGAGCC CGGCGGCGGA TCGGATCTGC AGAACATGAC CACCACCGCG CTGCCCGGCC CCGGCGGCGG CCTGGTGATC AACGGCGCCA AGACCTGGAT CAGCAACGCC CGGCGCTCCG GGCTGATCGC GTTGTTGTGC AAGACCGATC CCGACGCCAC CCCGCGGCAC CGGGGCATCT CGGTGGTGCT GGTGGAACAG GGCACCACCG GGCTGTCGGT GTCACGCGAT CTGCCCAAAC TCGGCTACAA GGGGGTGGAG TCCTGCGAGC TGGTCTTCGA CGACTGCCGG GTGCCGGCCT CGGCGATCCT CGGCGGTGAG CCCGGCAAGG GCTTCACGCA GATGATGAAG GGCCTGGAGA CCGGGCGCAT CCAGGTCGCC GCCCGCGCGC TGGGAGTGGC CACCGCCGCC CTGGAGGACN NNNNNNNCTA CGCCCAGCAG CGGGAGAGCT TCGGCCAACC GATCTGGAAG CATCAGGCCA TCGGCCACTA CCTGGCCGAC ATGGCCACCA AACTCACCGC CGCCCGCCAG CTCACCCGCT ACGCCGCCGA ACGCTACGAC AGCGGCCAGC GCTGCGACAT GGAGGCCGGC ATGGCCAAAC TGTTCGCCTC CGAGGTCGCC ATGGAGATCG CGCTCAACGC CGTGCGCATC CACGGCGGCT ACGGCTACTC CACCGAATTC GACGTGGAAC GCTACTTCCG CGACGCCCCG CTGATGATCG TCGGCGAAGG CACCAACGAG ATCCAGCGCA ACGTCATCGT CAGCCAACTC GTGGCCCGCA ATACTGTT

Details for MythA.01263.c.A1.GE28776

HARVESTED ON:	3/1/2010
SEQUENCED ON:	5/19/2010
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Validated AA Sequence

MVNVVLNDEE TMLVETVRAF IDRDVKPTVR EVEHANEYPQ AWIEQMKRIG IYGLAIPESY GGSPVSMPCY VEVTQELSRG WMSLAGAMGG HTVVAKLLTL FGTEEQKQRY LPAMATGEIR ATMALTEPGG GSDLQNMTTT ALPGPGGGLV INGAKTWISN ARRSGLIALL CKTDPDATPR HRGISVVLVE QGTTGLSVSR DLPKLGYKGV ESCELVFDDC RVPASAILGG EPGKGFTQMM KGLETGRIQV AARALGVATA ALEDALAYAQ QRESFGQPIW KHQAIGHYLA DMATKLTAAR QLTRYAAERY DSGQRCDMEA GMAKLFASEV AMEIALNAVR IHGGYGYSTE FDVERYFRDA PLMIVGEGTN EIQRNVIVSQ LVARNTV

Validated NT Sequence

ttattaaaca gtattgcggg ccacgagttg gctgacgatg acgttgcgct ggatctcgtt ggtgccttcg ccgacgatca tcagcggggc gtcgcggaag tagcgttcca cgtcgaattc ggtggagtag ccgtagccgc cgtggatgcg cacggcgttg agcgcgatct ccatggcgac ctcggaggcg aacagtttgg ccatgccggc ctccatgtcg cagcgctggc cgctgtcgta gcgttcggcg gcgtagcggg tgagctggcg ggcggcggtg agtttggtgg ccatgtcggc caggtagtgg ccgatggcct gatgcttcca gatcggttgg ccgaagctct cccgctgctg ggcgtaggcc agggcgtcct ccagggcggc ggtggccact cccagcgcgc gggcggcgac ctggatgcgc ccggtctcca ggcccttcat catctgcgtg aagcccttgc cgggctcacc gccgaggatc gccgaggccg gcacccggca gtcgtcgaag accagctcgc aggactccac ccccttgtag ccgagtttgg gcagatcgcg tgacaccgac agcccggtgg tgccctgttc caccagcacc accgagatgc cccggtgccg cggggtggcg tcgggatcgg tcttgcacaa caacgcgatc agcccggagc gccgggcgtt gctgatccag gtcttggcgc cgttgatcac caggccgccg ccggggccgg gcagcgcggt ggtggtcatg ttctgcagat ccgatccgcc gccgggctcg gtgagcgcca tggtcgcgcg gatctcgccg gtggccatcg ccggcaggta gcgctgtttc tgttcctcgg tgccgaacag cgtcaacagt ttggccacca cggtgtgccc gcccatcgcc ccggccaggc tcatccagcc gcgcgacagc tcctgggtca cctcgacgta acacggcatc gacaccggtg agccgccgta ggactccggg atcgccagcc cgtagatgcc gatgcgtttc atctgctcga tccacgcctg cggatattcg ttggcgtgtt cgacctcgcg cacggtgggc ttgacgtcgc ggtcgatgaa cgcccgcacc gtctcgacca gcatggtctc ttcatcgttg aggacgacat tcaccatcga accaggaccc tgggtctgag cttccaggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVNVVLNDE ETMLVETVRA FIDRDVKPTV REVEHANEYP QAWIEQMKRI GIYGLAIPES YGGSPVSMPC YVEVTQELSR GWMSLAGAMG GHTVVAKLLT LFGTEEQKQR YLPAMATGEI RATMALTEPG GGSDLQNMTT TALPGPGGGL VINGAKTWIS NARRSGLIAL LCKTDPDATP RHRGISVVLV EQGTTGLSVS RDLPKLGYKG VESCELVFDD CRVPASAILG GEPGKGFTQM MKGLETGRIQ VAARALGVAT AALEDXXXYA QQRESFGQPI WKHQAIGHYL ADMATKLTAA RQLTRYAAER YDSGQRCDME AGMAKLFASE VAMEIALNAV RIHGGYGYST EFDVERYFRD APLMIVGEGT NEIQRNVIVS QLVARNTV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaatgtc gtcctcaacg atgaagagac catgctggtc gagacggtgc gggcgttcat cgaccgcgac gtcaagccca ccgtgcgcga ggtcgaacac gccaacgaat atccgcaggc gtggatcgag cagatgaaac gcatcggcat ctacgggctg gcgatcccgg agtcctacgg cggctcaccg gtgtcgatgc cgtgttatgt cgaggtgacc caggagctgt cgcgcggctg gatgagcctg gccggggcga tgggcgggca caccgtggtg gccaaactgt tgacgctgtt cggcaccgag gaacagaaac agcgctacct gccggcgatg gccaccggcg agatccgcgc gaccatggcg ctcaccgagc ccggcggcgg atcggatctg cagaacatga ccaccaccgc gctgcccggc cccggcggcg gcctggtgat caacggcgcc aagacctgga tcagcaacgc ccggcgctcc gggctgatcg cgttgttgtg caagaccgat cccgacgcca ccccgcggca ccggggcatc tcggtggtgc tggtggaaca gggcaccacc gggctgtcgg tgtcacgcga tctgcccaaa ctcggctaca agggggtgga gtcctgcgag ctggtcttcg acgactgccg ggtgccggcc tcggcgatcc tcggcggtga gcccggcaag ggcttcacgc agatgatgaa gggcctggag accgggcgca tccaggtcgc cgcccgcgcg ctgggagtgg ccaccgccgc cctggaggac nnnnnnnnct acgcccagca gcgggagagc ttcggccaac cgatctggaa gcatcaggcc atcggccact acctggccga catggccacc aaactcaccg ccgcccgcca gctcacccgc tacgccgccg aacgctacga cagcggccag cgctgcgaca tggaggccgg catggccaaa ctgttcgcct ccgaggtcgc catggagatc gcgctcaacg ccgtgcgcat ccacggcggc tacggctact ccaccgaatt cgacgtggaa cgctacttcc gcgacgcccc gctgatgatc gtcggcgaag gcaccaacga gatccagcgc aacgtcatcg tcagccaact cgtggcccgc aatactgtta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MythA.01263.c.A1.PS00646

PURIFICATION DATe:	6/25/2010
CONCENTRATION:	154mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAATGTC GTCCTCAACG ATGAAGAGAC CATGCTGGTC GAGACGGTGC GGGCGTTCAT CGACCGCGAC GTCAAGCCCA CCGTGCGCGA GGTCGAACAC GCCAACGAAT ATCCGCAGGC GTGGATCGAG CAGATGAAAC GCATCGGCAT CTACGGGCTG GCGATCCCGG AGTCCTACGG CGGCTCACCG GTGTCGATGC CGTGTTATGT CGAGGTGACC CAGGAGCTGT CGCGCGGCTG GATGAGCCTG GCCGGGGCGA TGGGCGGGCA CACCGTGGTG GCCAAACTGT TGACGCTGTT CGGCACCGAG GAACAGAAAC AGCGCTACCT GCCGGCGATG GCCACCGGCG AGATCCGCGC GACCATGGCG CTCACCGAGC CCGGCGGCGG ATCGGATCTG CAGAACATGA CCACCACCGC GCTGCCCGGC CCCGGCGGCG GCCTGGTGAT CAACGGCGCC AAGACCTGGA TCAGCAACGC CCGGCGCTCC GGGCTGATCG CGTTGTTGTG CAAGACCGAT CCCGACGCCA CCCCGCGGCA CCGGGGCATC TCGGTGGTGC TGGTGGAACA GGGCACCACC GGGCTGTCGG TGTCACGCGA TCTGCCCAAA CTCGGCTACA AGGGGGTGGA GTCCTGCGAG CTGGTCTTCG ACGACTGCCG GGTGCCGGCC TCGGCGATCC TCGGCGGTGA GCCCGGCAAG GGCTTCACGC AGATGATGAA GGGCCTGGAG ACCGGGCGCA TCCAGGTCGC CGCCCGCGCG CTGGGAGTGG CCACCGCCGC CCTGGAGGAC NNNNNNNNCT ACGCCCAGCA GCGGGAGAGC TTCGGCCAAC CGATCTGGAA GCATCAGGCC ATCGGCCACT ACCTGGCCGA CATGGCCACC AAACTCACCG CCGCCCGCCA GCTCACCCGC TACGCCGCCG AACGCTACGA CAGCGGCCAG CGCTGCGACA TGGAGGCCGG CATGGCCAAA CTGTTCGCCT CCGAGGTCGC CATGGAGATC GCGCTCAACG CCGTGCGCAT CCACGGCGGC TACGGCTACT CCACCGAATT CGACGTGGAA CGCTACTTCC GCGACGCCCC GCTGATGATC GTCGGCGAAG GCACCAACGA GATCCAGCGC AACGTCATCG TCAGCCAACT CGTGGCCCGC AATACTGTTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A