SSGCID: Target Page

MysmA.00391.b

Short chain dehydrogenase

CENTER ID:	MysmA.00391.b
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00391.b.A1.GE28630	Full length( MysmA.00391.b )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

4WEC

DEPOSITED: 9/9/2014

DETERMINATION: XRay

Diffraction Image

CLONE: MysmA.00391.b.A1.GE28630

PROTEIN: MysmA.00391.b.A1.PS00660

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.2564
RefSeq:	YP_886935.1
UniProt:	A0QVJ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLTQRLAGK VAVITGGASG IGLATGRRLR AEGATVVVGD IDPTTGKAAA DELEGLFVPV DVSEQEAVDN LFDTAASTFG RVDIAFNNAG ISPPEDDLIE NTDLPAWQRV QDINLKSVYL SCRAALRHMV PAGKGSIINT ASFVAVMGSA TSQISYTASK GGVLAMSREL GVQYARQGIR VNALCPGPVN TPLLQELFAK DPERAARRLV HIPLGRFAEP EELAAAVAFL ASDDASFITG STFLVDGGIS SAYVTPL

NT Sequence

ATGGACCTGA CCCAACGCCT CGCCGGCAAG GTCGCCGTGA TCACCGGCGG CGCAAGCGGT ATCGGCCTGG CCACCGGCCG GCGGCTGCGC GCCGAGGGCG CCACCGTCGT GGTGGGTGAC ATCGACCCCA CGACCGGCAA GGCCGCCGCC GACGAACTCG AAGGCCTGTT CGTGCCCGTG GACGTCTCCG AGCAGGAGGC CGTCGACAAC CTGTTCGACA CCGCCGCTTC GACTTTCGGG CGGGTCGACA TCGCGTTCAA CAACGCGGGC ATCTCGCCAC CCGAGGACGA CCTGATCGAG AACACCGACC TGCCCGCCTG GCAGCGGGTC CAGGACATCA ACCTCAAGTC GGTGTACCTG TCGTGTCGCG CCGCCCTGCG GCACATGGTG CCCGCGGGCA AGGGGTCGAT CATCAACACC GCGTCGTTCG TGGCCGTGAT GGGTTCGGCG ACATCGCAGA TCTCCTACAC CGCATCCAAG GGCGGTGTGC TCGCGATGTC GCGTGAGCTC GGCGTGCAGT ACGCGCGCCA GGGCATCCGG GTCAACGCGT TGTGCCCGGG ACCGGTCAAC ACCCCGCTGC TGCAGGAGTT GTTCGCCAAG GATCCCGAGC GCGCCGCGCG CAGGCTCGTG CACATCCCGC TGGGCCGGTT CGCCGAACCC GAGGAACTCG CCGCCGCGGT CGCGTTCCTG GCCAGTGACG ACGCGTCGTT CATCACCGGG TCGACGTTCC TGGTCGACGG CGGGATCAGC TCCGCGTACG TCACGCCGCT G

Details for MysmA.00391.b.A1.GE28630

HARVESTED ON:	2/17/2010
SEQUENCED ON:	2/24/2010
EXPECTED MW:	29kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDLTQRLAG KVAVITGGAS GIGLATGRRL RAEGATVVVG DIDPTTGKAA ADELEGLFVP VDVSEQEAVD NLFDTAASTF GRVDIAFNNA GISPPEDDLI ENTDLPAWQR VQDINLKSVY LSCRAALRHM VPAGKGSIIN TASFVAVMGS ATSQISYTAS KGGVLAMSRE LGVQYARQGI RVNALCPGPV NTPLLQELFA KDPERAARRL VHIPLGRFAE PEELAAAVAF LASDDASFIT GSTFLVDGGI SSAYVTPL

Validated NT Sequence

tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggacct gacccaacgc ctcgccggca aggtcgccgt gatcaccggc ggcgcaagcg gtatcggcct ggccaccggc cggcggctgc gcgccgaggg cgccaccgtc gtggtgggtg acatcgaccc cacgaccggc aaggccgccg ccgacgaact cgaaggcctg ttcgtgcccg tggacgtctc cgagcaggag gccgtcgaca acctgttcga caccgccgct tcgactttcg ggcgggtcga catcgcgttc aacaacgcgg gcatctcgcc acccgaggac gacctgatcg agaacaccga cctgcccgcc tggcagcggg tccaggacat caacctcaag tcggtgtacc tgtcgtgtcg cgccgccctg cggcacatgg tgcccgcggg caaggggtcg atcatcaaca ccgcgtcgtt cgtggccgtg atgggttcgg cgacatcgca gatctcctac accgcatcca agggcggtgt gctcgcgatg tcgcgtgagc tcggcgtgca gtacgcgcgc cagggcatcc gggtcaacgc gttgtgcccg ggaccggtca acaccccgct gctgcaggag ttgttcgcca aggatcccga gcgcgccgcg cgcaggctcg tgcacatccc gctgggccgg ttcgccgaac ccgaggaact cgccgccgcg gtcgcgttcc tggccagtga cgacgcgtcg ttcatcaccg ggtcgacgtt cctggtcgac ggcgggatca gctccgcgta cgtcacgccg ctgtaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctgacc caacgcctcg ccggcaaggt cgccgtgatc accggcggcg caagcggtat cggcctggcc accggccggc ggctgcgcgc cgagggcgcc accgtcgtgg tgggtgacat cgaccccacg accggcaagg ccgccgccga cgaactcgaa ggcctgttcg tgcccgtgga cgtctccgag caggaggccg tcgacaacct gttcgacacc gccgcttcga ctttcgggcg ggtcgacatc gcgttcaaca acgcgggcat ctcgccaccc gaggacgacc tgatcgagaa caccgacctg cccgcctggc agcgggtcca ggacatcaac ctcaagtcgg tgtacctgtc gtgtcgcgcc gccctgcggc acatggtgcc cgcgggcaag gggtcgatca tcaacaccgc gtcgttcgtg gccgtgatgg gttcggcgac atcgcagatc tcctacaccg catccaaggg cggtgtgctc gcgatgtcgc gtgagctcgg cgtgcagtac gcgcgccagg gcatccgggt caacgcgttg tgcccgggac cggtcaacac cccgctgctg caggagttgt tcgccaagga tcccgagcgc gccgcgcgca ggctcgtgca catcccgctg ggccggttcg ccgaacccga ggaactcgcc gccgcggtcg cgttcctggc cagtgacgac gcgtcgttca tcaccgggtc gacgttcctg gtcgacggcg ggatcagctc cgcgtacgtc acgccgctga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a