MymaA.00391.a

Short chain membrane-associated dehydrogenase

CENTER ID: MymaA.00391.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00391.a.A1.GE30017 Full length( MymaA.00391.a ) 1 261
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00391.a.A1.PS00861 Full length( MymaA.00391.a ) 1 261

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3714
RefSeq: YP_001851744.1
UniProt: B2HIY2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDHRASRTF VVTGASSGIG LATVGRLLEQ GCAVVGADVV TPPHDLGPRF TFVTADVTDE DAVAGVFDAV PDRLDGVVHS AGVAGGGPVH LLARAEWDRV IGVNLTGTFL VAKAALARMI EQPRVDGERG SIVTLASIEG LEGTAGGSSY NAAKGGVVLL TKNIALDYGP SGIRANAICP GFIATPLAEG VFGMPGMEGP RASITNEHAL QRLGKPEEIA AMAAFLLSPD ASFVTGQAIA VDGGYTAGRD HGVVRLFGLP Q
NT Sequence
GTGCTCGATC ATCGGGCGTC CCGCACGTTT GTGGTAACCG GTGCGTCCTC GGGCATTGGC TTGGCCACCG TAGGCCGCTT GCTCGAACAA GGCTGCGCAG TTGTCGGTGC CGACGTCGTA ACCCCGCCCC ACGACCTCGG TCCGCGATTC ACGTTCGTGA CCGCCGACGT CACCGACGAG GACGCGGTCG CCGGCGTGTT CGACGCCGTG CCCGACCGCC TCGATGGGGT GGTGCATTCG GCTGGGGTGG CCGGTGGCGG CCCGGTGCAT CTGCTGGCGC GCGCTGAATG GGACCGAGTC ATTGGGGTCA ATCTCACCGG CACTTTTCTG GTAGCCAAGG CGGCGTTGGC CAGGATGATC GAGCAACCCC GGGTGGACGG CGAACGCGGC TCAATCGTGA CGCTGGCCAG CATCGAAGGT CTGGAGGGCA CCGCCGGCGG TAGTTCTTAC AACGCCGCCA AGGGCGGTGT TGTGTTGCTG ACCAAGAACA TTGCGCTCGA CTATGGGCCC AGTGGTATCC GGGCGAACGC GATCTGCCCG GGCTTCATCG CCACCCCCCT GGCCGAAGGG GTCTTCGGCA TGCCGGGAAT GGAGGGCCCG CGTGCGTCGA TCACCAACGA GCATGCGCTG CAACGATTGG GAAAGCCGGA GGAGATCGCG GCCATGGCTG CCTTCTTGCT CTCACCCGAC GCTTCCTTTG TGACAGGCCA GGCCATTGCC GTCGACGGCG GCTACACCGC CGGGCGCGAT CACGGGGTGG TTCGGCTCTT CGGTCTCCCA CAG
Details for MymaA.00391.a.A1.GE30017
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 28kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLDHRASR TFVVTGASSG IGLATVGRLL EQGCAVVGAD VVTPPHDLGP RFTFVTADVT DEDAVAGVFD AVPDRLDGVV HSAGVAGGGP VHLLARAEWD RVIGVNLTGT FLVAKAALAR MIEQPRVDGE RGSIVTLASI EGLEGTAGGS SYNAAKGGVV LLTKNIALDY GPSGIRANAI CPGFIATPLA EGVFGMPGME GPRASITNEH ALQRLGKPEE IAAMAAFLLS PDASFVTGQA IAVDGGYTAG RDHGVVRLFG LPL
Validated NT Sequence
ttataatggg agaccgaaga gccgaaccac cccgtgatcg cgcccggcgg tgtagccgcc gtcgacggca atggcctggc ctgtcacaaa ggaagcgtcg ggtgagagca agaaggcagc catggccgcg atctcctccg gctttcccaa tcgttgcagc gcatgctcgt tggtgatcga cgcacgcggg ccctccattc ccggcatgcc gaagacccct tcggccaggg gggtggcgat gaagcccggg cagatcgcgt tcgcccggat accactgggc ccatagtcga gcgcaatgtt cttggtcagc aacacaacac cgcccttggc ggcgttgtaa gaactaccgc cggcggtgcc ctccagacct tcgatgctgg ccagcgtcac gattgagccg cgttcgccgt ccacccgggg ttgctcgatc atcctggcca acgccgcctt ggctaccaga aaagtgccgg tgagattgac cccaatgact cggtcccatt cagcgcgcgc cagcagatgc accgggccgc caccggccac cccagccgaa tgcaccaccc catcgaggcg gtcgggcacg gcgtcgaaca cgccggcgac cgcgtcctcg tcggtgacgt cggcggtcac gaacgtgaat cgcggaccga ggtcgtgggg cggggttacg acgtcggcac cgacaactgc gcagccttgt tcgagcaagc ggcctacggt ggccaagcca atgcccgagg acgcaccggt taccacaaac gtgcgggacg cccgatgatc gagcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLDHRASR TFVVTGASSG IGLATVGRLL EQGCAVVGAD VVTPPHDLGP RFTFVTADVT DEDAVAGVFD AVPDRLDGVV HSAGVAGGGP VHLLARAEWD RVIGVNLTGT FLVAKAALAR MIEQPRVDGE RGSIVTLASI EGLEGTAGGS SYNAAKGGVV LLTKNIALDY GPSGIRANAI CPGFIATPLA EGVFGMPGME GPRASITNEH ALQRLGKPEE IAAMAAFLLS PDASFVTGQA IAVDGGYTAG RDHGVVRLFG LP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctcgat catcgggcgt cccgcacgtt tgtggtaacc ggtgcgtcct cgggcattgg cttggccacc gtaggccgct tgctcgaaca aggctgcgca gttgtcggtg ccgacgtcgt aaccccgccc cacgacctcg gtccgcgatt cacgttcgtg accgccgacg tcaccgacga ggacgcggtc gccggcgtgt tcgacgccgt gcccgaccgc ctcgatgggg tggtgcattc ggctggggtg gccggtggcg gcccggtgca tctgctggcg cgcgctgaat gggaccgagt cattggggtc aatctcaccg gcacttttct ggtagccaag gcggcgttgg ccaggatgat cgagcaaccc cgggtggacg gcgaacgcgg ctcaatcgtg acgctggcca gcatcgaagg tctggagggc accgccggcg gtagttctta caacgccgcc aagggcggtg ttgtgttgct gaccaagaac attgcgctcg actatgggcc cagtggtatc cgggcgaacg cgatctgccc gggcttcatc gccacccccc tggccgaagg ggtcttcggc atgccgggaa tggagggccc gcgtgcgtcg atcaccaacg agcatgcgct gcaacgattg ggaaagccgg aggagatcgc ggccatggct gccttcttgc tctcacccga cgcttccttt gtgacaggcc aggccattgc cgtcgacggc ggctacaccg ccgggcgcga tcacggggtg gttcggctct tcggtctccc aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MymaA.00391.a.A1.PS00861
PURIFICATION DATe: 11/16/2010
CONCENTRATION: 33.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLDHRASR TFVVTGASSG IGLATVGRLL EQGCAVVGAD VVTPPHDLGP RFTFVTADVT DEDAVAGVFD AVPDRLDGVV HSAGVAGGGP VHLLARAEWD RVIGVNLTGT FLVAKAALAR MIEQPRVDGE RGSIVTLASI EGLEGTAGGS SYNAAKGGVV LLTKNIALDY GPSGIRANAI CPGFIATPLA EGVFGMPGME GPRASITNEH ALQRLGKPEE IAAMAAFLLS PDASFVTGQA IAVDGGYTAG RDHGVVRLFG LPL
Validated NT Sequence
ttataatggg agaccgaaga gccgaaccac cccgtgatcg cgcccggcgg tgtagccgcc gtcgacggca atggcctggc ctgtcacaaa ggaagcgtcg ggtgagagca agaaggcagc catggccgcg atctcctccg gctttcccaa tcgttgcagc gcatgctcgt tggtgatcga cgcacgcggg ccctccattc ccggcatgcc gaagacccct tcggccaggg gggtggcgat gaagcccggg cagatcgcgt tcgcccggat accactgggc ccatagtcga gcgcaatgtt cttggtcagc aacacaacac cgcccttggc ggcgttgtaa gaactaccgc cggcggtgcc ctccagacct tcgatgctgg ccagcgtcac gattgagccg cgttcgccgt ccacccgggg ttgctcgatc atcctggcca acgccgcctt ggctaccaga aaagtgccgg tgagattgac cccaatgact cggtcccatt cagcgcgcgc cagcagatgc accgggccgc caccggccac cccagccgaa tgcaccaccc catcgaggcg gtcgggcacg gcgtcgaaca cgccggcgac cgcgtcctcg tcggtgacgt cggcggtcac gaacgtgaat cgcggaccga ggtcgtgggg cggggttacg acgtcggcac cgacaactgc gcagccttgt tcgagcaagc ggcctacggt ggccaagcca atgcccgagg acgcaccggt taccacaaac gtgcgggacg cccgatgatc gagcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLDHRASR TFVVTGASSG IGLATVGRLL EQGCAVVGAD VVTPPHDLGP RFTFVTADVT DEDAVAGVFD AVPDRLDGVV HSAGVAGGGP VHLLARAEWD RVIGVNLTGT FLVAKAALAR MIEQPRVDGE RGSIVTLASI EGLEGTAGGS SYNAAKGGVV LLTKNIALDY GPSGIRANAI CPGFIATPLA EGVFGMPGME GPRASITNEH ALQRLGKPEE IAAMAAFLLS PDASFVTGQA IAVDGGYTAG RDHGVVRLFG LP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCTCGAT CATCGGGCGT CCCGCACGTT TGTGGTAACC GGTGCGTCCT CGGGCATTGG CTTGGCCACC GTAGGCCGCT TGCTCGAACA AGGCTGCGCA GTTGTCGGTG CCGACGTCGT AACCCCGCCC CACGACCTCG GTCCGCGATT CACGTTCGTG ACCGCCGACG TCACCGACGA GGACGCGGTC GCCGGCGTGT TCGACGCCGT GCCCGACCGC CTCGATGGGG TGGTGCATTC GGCTGGGGTG GCCGGTGGCG GCCCGGTGCA TCTGCTGGCG CGCGCTGAAT GGGACCGAGT CATTGGGGTC AATCTCACCG GCACTTTTCT GGTAGCCAAG GCGGCGTTGG CCAGGATGAT CGAGCAACCC CGGGTGGACG GCGAACGCGG CTCAATCGTG ACGCTGGCCA GCATCGAAGG TCTGGAGGGC ACCGCCGGCG GTAGTTCTTA CAACGCCGCC AAGGGCGGTG TTGTGTTGCT GACCAAGAAC ATTGCGCTCG ACTATGGGCC CAGTGGTATC CGGGCGAACG CGATCTGCCC GGGCTTCATC GCCACCCCCC TGGCCGAAGG GGTCTTCGGC ATGCCGGGAA TGGAGGGCCC GCGTGCGTCG ATCACCAACG AGCATGCGCT GCAACGATTG GGAAAGCCGG AGGAGATCGC GGCCATGGCT GCCTTCTTGC TCTCACCCGA CGCTTCCTTT GTGACAGGCC AGGCCATTGC CGTCGACGGC GGCTACACCG CCGGGCGCGA TCACGGGGTG GTTCGGCTCT TCGGTCTCCC AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA