MysmA.00277.c

LipW protein

CENTER ID: MysmA.00277.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00277.c.A1.PS00565 Full length( MysmA.00277.c ) 1 310

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2534
RefSeq: YP_886905.1
UniProt: A0QVG7

Orthologues for Rv1400c

Species % Identity % Coverage Clones Proteins Structures
ulcerans 43 74 1 0 0
smegmatis 40 78 0 1 0
marinum 39 88 1 1 1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAHRSGWTER LDPALREFAG ARTDLSPETL AVVRASIDRR RRESAQTLDT FGVAIAGGRA ALGRRSVPVR IYRGGPSPAP VVVYCHSGAF VLGNLDVDQR QCVELARRGQ CTVVAMDYRL APEHPYPAAL DDAAMVLAWV AAQAGELDVD PDRIAVAGSS AGGALAALLA QRSATGAAPP IVFQLLHQPV LDDRPTRSKR EFVDTPGFDG PATVAMWRHW LAGRAAPDGA VPARATEISD MPAAFISCSE LDPLRDEALD HARRLIDGGV STELHVFAGT CHGFDSLLPE WEVSQELFTL QGAALRRALR
NT Sequence
ATGGCTCACA GGTCCGGATG GACCGAGCGC CTGGATCCCG CGCTGCGCGA ATTCGCCGGT GCCCGCACCG ATCTGTCGCC CGAAACCCTT GCGGTGGTGC GGGCCTCGAT CGACCGGCGT CGCCGTGAAT CGGCGCAGAC GCTCGACACG TTCGGCGTGG CCATCGCAGG CGGCCGGGCC GCGCTGGGCC GGCGCAGTGT GCCGGTGCGC ATCTACCGTG GCGGCCCGTC GCCTGCACCC GTGGTCGTGT ACTGCCACTC GGGCGCGTTC GTGCTGGGCA ACCTCGACGT CGACCAGCGG CAATGCGTCG AACTGGCGCG GCGTGGGCAG TGCACGGTCG TGGCCATGGA CTACCGCCTC GCCCCCGAAC ATCCGTACCC CGCAGCGCTC GACGACGCCG CGATGGTGCT GGCCTGGGTG GCCGCGCAGG CCGGTGAACT CGACGTCGAC CCGGATCGGA TCGCGGTCGC GGGCAGCAGT GCCGGTGGTG CGCTGGCCGC GTTGCTGGCA CAGCGGTCCG CGACGGGCGC CGCGCCTCCA ATCGTGTTCC AGTTGCTGCA CCAGCCGGTG CTCGACGACC GCCCGACCCG CTCCAAGCGC GAATTCGTCG ACACACCCGG TTTCGACGGC CCCGCGACGG TCGCGATGTG GCGTCACTGG CTGGCCGGCC GCGCGGCGCC CGACGGCGCC GTACCGGCCC GCGCCACCGA GATCTCGGAC ATGCCTGCGG CTTTCATCAG CTGCTCGGAA CTGGACCCGC TGCGCGACGA AGCACTCGAC CATGCGCGCA GGCTGATCGA CGGCGGCGTG TCCACCGAGC TGCACGTGTT CGCGGGTACC TGCCACGGAT TCGACTCACT GCTGCCCGAG TGGGAGGTCA GTCAGGAACT GTTCACCCTG CAGGGGGCGG CACTGCGCCG GGCGCTGCGC
Details for MysmA.00277.c.A1.PS00565
PURIFICATION DATe: 4/23/2010
CONCENTRATION: 16.41mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 91
PERCENT COVERAGE: 86
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAHRSGWTE RLDPALREFA GARTDLSPET LAVVRASIDR RRRESAQTLD TFGVAIAGGR AALGRRSVPV RIYRGGPSPA PVVVYCHSGA FVLGNLDVDQ RQCVELARRG QCTVVAMDYR LAPEHPYPAA LDDAAMVLAW VAAQAGELDV DPDRIAVAGS SAGGALAALL AQRSATGAAP PIVFQLLHQP VLDDRPTRSK REFVDTPGFD GPATVAMWRH WLAGRAAPDA PYRPAPPRSR TXLRLSSAAX NWTRCATXHS TMXXX
Validated NT Sequence
tnnactttaa ganggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gctcacaggt ccggatggac cgagcgcctg gatcccgcgc tgcgcgaatt cgccggtgcc cgcaccgatc tgtcgcccga aacccttgcg gtggtgcggg cctcgatcga ccggcgtcgc cgtgaatcgg cgcagacgct cgacacgttc ggcgtggcca tcgcaggcgg ccgggccgcg ctgggccggc gcagtgtgcc ggtgcgcatc taccgtggcg gcccgtcgcc tgcacccgtg gtcgtgtact gccactcggg cgcgttcgtg ctgggcaacc tcgacgtcga ccagcggcaa tgcgtcgaac tggcgcggcg tgggcagtgc acggtcgtgg ccatggacta ccgcctcgcc cccgaacatc cgtaccccgc agcgctcgac gacgccgcga tggtgctggc ctgggtggcc gcgcaggccg gtgaactcga cgtcgacccg gatcggatcg cggtcgcggg cagcagtgcc ggtggtgcgc tggccgcgtt gctggcacag cggtccgcga cgggcgccgc gcctccnatc gtgttccagt tgctgcacca gccggtgctc gacgaccgcc cgacccgctc caagcgcgaa ttcgtcgaca cacccggttt cgacggcccc gcgacggtcg cgatgtggcg tcactggctg gccggccgcg cggcgcccga cgcgccgtac cggcccgcgc caccgagatc tcggacatgn ctgcggcttt catcagctgc tcngaactgg acccgntgcg cgacnaanca ctcgaccatg cnnnnnngct gatcnacngc ggcgtgtccn ncgagctgca nntgtnnnnc ntacctgnca cggattcnac tcactgctgn cnantggnan gtcantcang nantnnnnnc ccngnngggg nngcactgnn ncgggnnnct gcnnnnnaat aancagcacn annnnnttct gcanccaagc ttctcnnngn nnnnncnnct ancnaanncc cnaannnnnn ntnnnntngn cnnnnnnc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAHRSGWTE RLDPALREFA GARTDLSPET LAVVRASIDR RRRESAQTLD TFGVAIAGGR AALGRRSVPV RIYRGGPSPA PVVVYCHSGA FVLGNLDVDQ RQCVELARRG QCTVVAMDYR LAPEHPYPAA LDDAAMVLAW VAAQAGELDV DPDRIAVAGS SAGGALAALL AQRSATGAAP PIVFQLLHQP VLDDRPTRSK REFVDTPGFD GPATVAMWRH WLAGRAAPDG AVPARATEIS DMPAAFISCS ELDPLRDEAL DHARRLIDGG VSTELHVFAG TCHGFDSLLP EWEVSQELFT LQGAALRRAL R
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTCACAGG TCCGGATGGA CCGAGCGCCT GGATCCCGCG CTGCGCGAAT TCGCCGGTGC CCGCACCGAT CTGTCGCCCG AAACCCTTGC GGTGGTGCGG GCCTCGATCG ACCGGCGTCG CCGTGAATCG GCGCAGACGC TCGACACGTT CGGCGTGGCC ATCGCAGGCG GCCGGGCCGC GCTGGGCCGG CGCAGTGTGC CGGTGCGCAT CTACCGTGGC GGCCCGTCGC CTGCACCCGT GGTCGTGTAC TGCCACTCGG GCGCGTTCGT GCTGGGCAAC CTCGACGTCG ACCAGCGGCA ATGCGTCGAA CTGGCGCGGC GTGGGCAGTG CACGGTCGTG GCCATGGACT ACCGCCTCGC CCCCGAACAT CCGTACCCCG CAGCGCTCGA CGACGCCGCG ATGGTGCTGG CCTGGGTGGC CGCGCAGGCC GGTGAACTCG ACGTCGACCC GGATCGGATC GCGGTCGCGG GCAGCAGTGC CGGTGGTGCG CTGGCCGCGT TGCTGGCACA GCGGTCCGCG ACGGGCGCCG CGCCTCCAAT CGTGTTCCAG TTGCTGCACC AGCCGGTGCT CGACGACCGC CCGACCCGCT CCAAGCGCGA ATTCGTCGAC ACACCCGGTT TCGACGGCCC CGCGACGGTC GCGATGTGGC GTCACTGGCT GGCCGGCCGC GCGGCGCCCG ACGGCGCCGT ACCGGCCCGC GCCACCGAGA TCTCGGACAT GCCTGCGGCT TTCATCAGCT GCTCGGAACT GGACCCGCTG CGCGACGAAG CACTCGACCA TGCGCGCAGG CTGATCGACG GCGGCGTGTC CACCGAGCTG CACGTGTTCG CGGGTACCTG CCACGGATTC GACTCACTGC TGCCCGAGTG GGAGGTCAGT CAGGAACTGT TCACCCTGCA GGGGGCGGCA CTGCGCCGGG CGCTGCGCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA