MymaA.00277.c
Esterase LipW
| CENTER ID: | MymaA.00277.c |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00277.c.A1.GE29983 | Full length( MymaA.00277.c ) | 1 | 313 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00277.c.A1.PS00815 | Full length( MymaA.00277.c ) | 1 | 313 |
Structures
3QH4
DEPOSITED: 1/25/2011
DETERMINATION: XRay
CLONE: MymaA.00277.c.A1.GE29983
PROTEIN: MymaA.00277.c.A1.PS00815
Publications by SSGCID
Structural basis for the strict substrate selectivity of the mycobacterial hydrolase LipW
Abendroth J, Edwards TE, McKary MG, Johnson RJ
Biochemistry - 2016
volume 55, issue 51, pages 7099-7111
PMID: 27936614; PMCID: PMC5482207
PDB:
3QH4
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126961 |
| PATRIC ID: | fig|216594.6.peg.445 |
| RefSeq: | YP_001848726.1 |
| UniProt: | B2HLX2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQPEAVDRL DPLLRAVATA RIDFTAESIL TIRESMNQRR REAAATETAA AGVAVADDVV TGEAGRPVPV RIYRAAPTPA PVVVYCHAGG FALGNLDTDH RQCLELARRA RCAVVSVDYR LAPEHPYPAA LHDAIEVLTW VVGNATRLGF DARRLAVAGS SAGATLAAGL AHGAADGSLP PVIFQLLHQP VLDDRPTASR SEFRATPAFD GEAASLMWRH YLAGQTPSPE SVPGRRGQLA GLPATLITCG EIDPFRDEVL DYAQRLLGAG VSTELHIFPR ACHGFDSLLP EWTTSQRLFA MQGHALADAF YPD
NT Sequence
GTGACCCAAC CCGAGGCCGT CGATCGGCTG GACCCCTTGC TGCGCGCCGT CGCGACGGCG CGAATCGACT TCACCGCGGA GTCGATCCTG ACGATCCGCG AGTCGATGAA CCAGCGCCGC CGGGAGGCGG CCGCGACCGA GACGGCCGCC GCCGGGGTAG CGGTGGCCGA CGATGTTGTC ACCGGTGAGG CCGGCAGGCC GGTGCCGGTC CGCATCTACC GGGCTGCCCC GACGCCGGCG CCGGTAGTCG TCTACTGCCA CGCGGGTGGC TTCGCCTTGG GAAATCTCGA TACCGACCAC CGGCAGTGCC TGGAGCTGGC TCGTCGTGCC CGCTGTGCGG TGGTTTCGGT CGACTACCGG CTGGCGCCCG AACACCCCTA CCCGGCGGCA TTGCACGACG CGATCGAGGT GCTGACGTGG GTGGTTGGCA ACGCGACACG GTTGGGATTC GACGCGCGGC GGCTGGCGGT GGCCGGTAGC AGCGCCGGCG CCACCCTGGC CGCCGGCCTG GCGCACGGCG CCGCGGACGG GTCGCTGCCA CCGGTCATCT TCCAGCTGCT GCACCAGCCG GTGCTCGACG ATCGCCCCAC CGCATCCCGG TCGGAGTTCC GCGCCACCCC GGCCTTCGAT GGTGAGGCGG CGAGTCTCAT GTGGCGTCAT TACCTGGCCG GGCAGACACC TTCACCGGAG TCGGTCCCGG GCCGGCGCGG TCAACTGGCA GGCCTGCCAG CGACGTTGAT CACCTGCGGC GAGATCGATC CGTTTCGCGA CGAAGTGCTC GATTACGCGC AGCGGCTGCT GGGAGCCGGG GTATCCACCG AACTGCACAT CTTCCCGCGC GCCTGCCACG GGTTCGATTC GCTACTACCG GAATGGACGA CGTCGCAGCG GCTGTTCGCG ATGCAGGGTC ATGCCCTGGC GGACGCGTTC TACCCGGAT
Details for MymaA.00277.c.A1.GE29983
| HARVESTED ON: | 7/13/2010 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMVTQPEA VDRLDPLLRA VATARIDFTA ESILTIRESM NQRRREAAAT ETAAAGVAVA DDVVTGEAGR PVPVRIYRAA PTPAPVVVYC HAGGFALGNL DTDHRQCLEL ARRARCAVVS VDYRLAPEHP YPAALHDAIE VLTWVVGNAT RLGFDARRLA VAGSSAGATL AAGLAHGAAD GSLPPVIFQL LHQPVLDDRP TASRSEFRAT PAFDGEAASL MWRHYLAGQT PSPESVPGRR GQLAGLPATL ITCGEIDPFR DEVLDYAQRL LGAGVSTELH IFPRACHGFD SLLPEWTTSQ RLFAMQGHAL ADAF
Validated NT Sequence
agaacgcgtc cgccagggca tgaccctgca tcgcgaacag ccgctgcgac gtcgtccatt ccggtagtag cgaatcgaac ccgtggcagg cgcgcgggaa gatgtgcagt tcggtggata ccccggctcc cagcagccgc tgcgcgtaat cgagcacttc gtcgcgaaac ggatcgatct cgccgcaggt gatcaacgtc gctggcaggc ctgccagttg accgcgccgg cccgggaccg actccggtga aggtgtctgc ccggccaggt aatgacgcca catgagactc gccgcctcac catcgaaggc cggggtggcg cggaactccg accgggatgc ggtggggcga tcgtcgagca ccggctggtg cagcagctgg aagatgaccg gtggcagcga cccgtccgcg gcgccgtgcg ccaggccggc ggccagggtg gcgccggcgc tgctaccggc caccgccagc cgccgcgcgt cgaatcccaa ccgtgtcgcg ttgccaacca cccacgtcag cacctcgatc gcgtcgtgca atgccgccgg gtaggggtgt tcgggcgcca gccggtagtc gaccgaaacc accgcacagc gggcacgacg agccagctcc aggcactgcc ggtggtcggt atcgagattt cccaaggcga agccacccgc gtggcagtag acgactaccg gcgccggcgt cggggcagcc cggtagatgc ggaccggcac cggcctgccg gcctcaccgg tgacaacatc gtcggccacc gctaccccgg cggcggccgt ctcggtcgcg gccgcctccc ggcggcgctg gttcatcgac tcgcggatcg tcaggatcga ctccgcggtg aagtcgattc gcgccgtcgc gacggcgcgc agcaaggggt ccagccgatc gacggcctcg ggttgggtca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTQPEAVD RLDPLLRAVA TARIDFTAES ILTIRESMNQ RRREAAATET AAAGVAVADD VVTGEAGRPV PVRIYRAAPT PAPVVVYCHA GGFALGNLDT DHRQCLELAR RARCAVVSVD YRLAPEHPYP AALHDAIEVL TWVVGNATRL GFDARRLAVA GSSAGATLAA GLAHGAADGS LPPVIFQLLH QPVLDDRPTA SRSEFRATPA FDGEAASLMW RHYLAGQTPS PESVPGRRGQ LAGLPATLIT CGEIDPFRDE VLDYAQRLLG AGVSTELHIF PRACHGFDSL LPEWTTSQRL FAMQGHALAD AFYP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacccaa cccgaggccg tcgatcggct ggaccccttg ctgcgcgccg tcgcgacggc gcgaatcgac ttcaccgcgg agtcgatcct gacgatccgc gagtcgatga accagcgccg ccgggaggcg gccgcgaccg agacggccgc cgccggggta gcggtggccg acgatgttgt caccggtgag gccggcaggc cggtgccggt ccgcatctac cgggctgccc cgacgccggc gccggtagtc gtctactgcc acgcgggtgg cttcgccttg ggaaatctcg ataccgacca ccggcagtgc ctggagctgg ctcgtcgtgc ccgctgtgcg gtggtttcgg tcgactaccg gctggcgccc gaacacccct acccggcggc attgcacgac gcgatcgagg tgctgacgtg ggtggttggc aacgcgacac ggttgggatt cgacgcgcgg cggctggcgg tggccggtag cagcgccggc gccaccctgg ccgccggcct ggcgcacggc gccgcggacg ggtcgctgcc accggtcatc ttccagctgc tgcaccagcc ggtgctcgac gatcgcccca ccgcatcccg gtcggagttc cgcgccaccc cggccttcga tggtgaggcg gcgagtctca tgtggcgtca ttacctggcc gggcagacac cttcaccgga gtcggtcccg ggccggcgcg gtcaactggc aggcctgcca gcgacgttga tcacctgcgg cgagatcgat ccgtttcgcg acgaagtgct cgattacgcg cagcggctgc tgggagccgg ggtatccacc gaactgcaca tcttcccgcg cgcctgccac gggttcgatt cgctactacc ggaatggacg acgtcgcagc ggctgttcgc gatgcagggt catgccctgg cggacgcgtt ctacccgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MymaA.00277.c.A1.PS00815
| PURIFICATION DATe: | 10/18/2010 |
| CONCENTRATION: | 55.55mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVTQPEA VDRLDPLLRA VATARIDFTA ESILTIRESM NQRRREAAAT ETAAAGVAVA DDVVTGEAGR PVPVRIYRAA PTPAPVVVYC HAGGFALGNL DTDHRQCLEL ARRARCAVVS VDYRLAPEHP YPAALHDAIE VLTWVVGNAT RLGFDARRLA VAGSSAGATL AAGLAHGAAD GSLPPVIFQL LHQPVLDDRP TASRSEFRAT PAFDGEAASL MWRHYLAGQT PSPESVPGRR GQLAGLPATL ITCGEIDPFR DEVLDYAQRL LGAGVSTELH IFPRACHGFD SLLPEWTTSQ RLFAMQGHAL ADAF
Validated NT Sequence
agaacgcgtc cgccagggca tgaccctgca tcgcgaacag ccgctgcgac gtcgtccatt ccggtagtag cgaatcgaac ccgtggcagg cgcgcgggaa gatgtgcagt tcggtggata ccccggctcc cagcagccgc tgcgcgtaat cgagcacttc gtcgcgaaac ggatcgatct cgccgcaggt gatcaacgtc gctggcaggc ctgccagttg accgcgccgg cccgggaccg actccggtga aggtgtctgc ccggccaggt aatgacgcca catgagactc gccgcctcac catcgaaggc cggggtggcg cggaactccg accgggatgc ggtggggcga tcgtcgagca ccggctggtg cagcagctgg aagatgaccg gtggcagcga cccgtccgcg gcgccgtgcg ccaggccggc ggccagggtg gcgccggcgc tgctaccggc caccgccagc cgccgcgcgt cgaatcccaa ccgtgtcgcg ttgccaacca cccacgtcag cacctcgatc gcgtcgtgca atgccgccgg gtaggggtgt tcgggcgcca gccggtagtc gaccgaaacc accgcacagc gggcacgacg agccagctcc aggcactgcc ggtggtcggt atcgagattt cccaaggcga agccacccgc gtggcagtag acgactaccg gcgccggcgt cggggcagcc cggtagatgc ggaccggcac cggcctgccg gcctcaccgg tgacaacatc gtcggccacc gctaccccgg cggcggccgt ctcggtcgcg gccgcctccc ggcggcgctg gttcatcgac tcgcggatcg tcaggatcga ctccgcggtg aagtcgattc gcgccgtcgc gacggcgcgc agcaaggggt ccagccgatc gacggcctcg ggttgggtca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTQPEAVD RLDPLLRAVA TARIDFTAES ILTIRESMNQ RRREAAATET AAAGVAVADD VVTGEAGRPV PVRIYRAAPT PAPVVVYCHA GGFALGNLDT DHRQCLELAR RARCAVVSVD YRLAPEHPYP AALHDAIEVL TWVVGNATRL GFDARRLAVA GSSAGATLAA GLAHGAADGS LPPVIFQLLH QPVLDDRPTA SRSEFRATPA FDGEAASLMW RHYLAGQTPS PESVPGRRGQ LAGLPATLIT CGEIDPFRDE VLDYAQRLLG AGVSTELHIF PRACHGFDSL LPEWTTSQRL FAMQGHALAD AFYP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCCAA CCCGAGGCCG TCGATCGGCT GGACCCCTTG CTGCGCGCCG TCGCGACGGC GCGAATCGAC TTCACCGCGG AGTCGATCCT GACGATCCGC GAGTCGATGA ACCAGCGCCG CCGGGAGGCG GCCGCGACCG AGACGGCCGC CGCCGGGGTA GCGGTGGCCG ACGATGTTGT CACCGGTGAG GCCGGCAGGC CGGTGCCGGT CCGCATCTAC CGGGCTGCCC CGACGCCGGC GCCGGTAGTC GTCTACTGCC ACGCGGGTGG CTTCGCCTTG GGAAATCTCG ATACCGACCA CCGGCAGTGC CTGGAGCTGG CTCGTCGTGC CCGCTGTGCG GTGGTTTCGG TCGACTACCG GCTGGCGCCC GAACACCCCT ACCCGGCGGC ATTGCACGAC GCGATCGAGG TGCTGACGTG GGTGGTTGGC AACGCGACAC GGTTGGGATT CGACGCGCGG CGGCTGGCGG TGGCCGGTAG CAGCGCCGGC GCCACCCTGG CCGCCGGCCT GGCGCACGGC GCCGCGGACG GGTCGCTGCC ACCGGTCATC TTCCAGCTGC TGCACCAGCC GGTGCTCGAC GATCGCCCCA CCGCATCCCG GTCGGAGTTC CGCGCCACCC CGGCCTTCGA TGGTGAGGCG GCGAGTCTCA TGTGGCGTCA TTACCTGGCC GGGCAGACAC CTTCACCGGA GTCGGTCCCG GGCCGGCGCG GTCAACTGGC AGGCCTGCCA GCGACGTTGA TCACCTGCGG CGAGATCGAT CCGTTTCGCG ACGAAGTGCT CGATTACGCG CAGCGGCTGC TGGGAGCCGG GGTATCCACC GAACTGCACA TCTTCCCGCG CGCCTGCCAC GGGTTCGATT CGCTACTACC GGAATGGACG ACGTCGCAGC GGCTGTTCGC GATGCAGGGT CATGCCCTGG CGGACGCGTT CTACCCGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA