MyleA.00184.a
PROBABLE PHOSPHOGLYCERATE MUTASE 1 GPM1 (PHOSPHOGLYCEROMUTASE) (PGAM) (BPG-DEPENDENT PGAM)
| CENTER ID: | MyleA.00184.a |
| ORGANISM: | Mycobacterium leprae Br4923 |
| ASSOCIATED DISEASE: | Hansen's disease, leprosy |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyleA.00184.a.A1.GE34159 | full length | 1 | 247 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyleA.00184.a.A1.PS01437 | full length | 1 | 247 |
Structures
4EO9
DEPOSITED: 4/13/2012
DETERMINATION: XRay
CLONE: MyleA.00184.a.A1.GE34159
PROTEIN: MyleA.00184.a.A1.PS01437
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|561304.4.peg.4708 |
| RefSeq: | YP_002504212.1 |
| UniProt: | B8ZT86 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQQGNTATLI LLRHGESDWN ARNLFTGWVD VGLTDKGRAE AVRSGELLAE HNLLPDVLYT SLLRRAITTA HLALDTADWL WIPVRRSWRL NERHYGALQG LDKAVTKARY GEERFMAWRR SYDTPPPPIE KGSEFSQDAD PRYTDIGGGP LTECLADVVT RFLPYFTDVI VPDLRTGRTV LIVAHGNSLR ALVKHLDEMS DDEVVGLNVP TGIPLRYDLD ADLRPVVPGG TYLDPEAAAA VISQARP
NT Sequence
atgcaacagg gtaacactgc tacgctgatt ctactccgtc atggcgagag tgactggaat gcccggaatc tgttcaccgg ttgggtggac gtcgggctaa ccgacaaggg tcgagcggag gcggtccgca gcggtgagtt gcttgccgag cacaatctgt tgcccgacgt gttgtacacg tcgctgttgc gccgcgctat caccactgcg catctggcgt tagacaccgc cgactggcta tggattccgg tccgtcgcag ctggcggctc aatgaacgtc actacggcgc gcttcagggt ctggacaagg ccgtgaccaa ggcccgctac ggcgaagaac gattcatggc ttggcgccgc agctacgaca ctccgccacc accgatcgag aagggtagcg agttcagcca ggatgctgac cctcgctaca ccgacattgg cggcgggccg ctgaccgaat gcctggctga tgtggtgacc cgcttcctgc cgtatttcac cgacgtgata gtccccgatc tgcggaccgg cagaacggtg ctcattgtgg cgcacggcaa ctcgctgcgc gcgctggtca aacacctaga cgagatgtcc gacgacgagg tcgtcgggct taacgttccg actggcattc cgctgcgcta tgatctagat gccgatctgc gtccggtggt acccggtggt acctacctcg accccgaggc ggccgctgcg gtaatcagtc aagcgcgccc gtaa
Details for MyleA.00184.a.A1.GE34159
| HARVESTED ON: | 12/6/2011 |
| SEQUENCED ON: | 12/13/2011 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQQGNTATL ILLRHGESDW NARNLFTGWV DVGLTDKGRA EAVRSGELLA EHNLLPDVLY TSLLRRAITT AHLALDTADW LWIPVRRSWR LNERHYGALQ GLDKAVTKAR YGEERFMAWR RSYDTPPPPI EKGSEFSQDA DPRYTDIGGG PLTECLADVV TRFLPYFTDV IVPDLRTGRT VLIVAHGNSL RALVKHLDEM SDDEVVGLNV PTGIPLRYDL DADLRPVVPG GTYLDPEAAA AVISQARP
Validated NT Sequence
ttattacggg cgcgcttgac tgattaccgc agcggccgcc tcggggtcga ggtaggtacc accgggtacc accggacgca gatcggcatc tagatcatag cgcagcggaa tgccagtcgg aacgttaagc ccgacgacct cgtcgtcgga catctcgtct aggtgtttga ccagcgcgcg cagcgagttg ccgtgcgcca caatgagcac cgttctgccg gtccgcagat cggggactat cacgtcggtg aaatacggca ggaagcgggt caccacatca gccaggcatt cggtcagcgg cccgccgcca atgtcggtgt agcgagggtc agcatcctgg ctgaactcgc tacccttctc gatcggtggt ggcggagtgt cgtagctgcg gcgccaagcc atgaatcgtt cttcgccgta gcgggccttg gtcacggcct tgtccagacc ctgaagcgcg ccgtagtgac gttcattgag ccgccagctg cgacggaccg gaatccatag ccagtcggcg gtgtctaacg ccagatgcgc agtggtgata gcgcggcgca acagcgacgt gtacaacacg tcgggcaaca gattgtgctc ggcaagcaac tcaccgctgc ggaccgcctc cgctcgaccc ttgtcggtta gcccgacgtc cacccaaccg gtgaacagat tccgggcatt ccagtcactc tcgccatgac ggagtagaat cagcgtagca gtgttaccct gttgcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQQGNTATL ILLRHGESDW NARNLFTGWV DVGLTDKGRA EAVRSGELLA EHNLLPDVLY TSLLRRAITT AHLALDTADW LWIPVRRSWR LNERHYGALQ GLDKAVTKAR YGEERFMAWR RSYDTPPPPI EKGSEFSQDA DPRYTDIGGG PLTECLADVV TRFLPYFTDV IVPDLRTGRT VLIVAHGNSL RALVKHLDEM SDDEVVGLNV PTGIPLRYDL DADLRPVVPG GTYLDPEAAA AVISQARP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaacagggt aacactgcta cgctgattct actccgtcat ggcgagagtg actggaatgc ccggaatctg ttcaccggtt gggtggacgt cgggctaacc gacaagggtc gagcggaggc ggtccgcagc ggtgagttgc ttgccgagca caatctgttg cccgacgtgt tgtacacgtc gctgttgcgc cgcgctatca ccactgcgca tctggcgtta gacaccgccg actggctatg gattccggtc cgtcgcagct ggcggctcaa tgaacgtcac tacggcgcgc ttcagggtct ggacaaggcc gtgaccaagg cccgctacgg cgaagaacga ttcatggctt ggcgccgcag ctacgacact ccgccaccac cgatcgagaa gggtagcgag ttcagccagg atgctgaccc tcgctacacc gacattggcg gcgggccgct gaccgaatgc ctggctgatg tggtgacccg cttcctgccg tatttcaccg acgtgatagt ccccgatctg cggaccggca gaacggtgct cattgtggcg cacggcaact cgctgcgcgc gctggtcaaa cacctagacg agatgtccga cgacgaggtc gtcgggctta acgttccgac tggcattccg ctgcgctatg atctagatgc cgatctgcgt ccggtggtac ccggtggtac ctacctcgac cccgaggcgg ccgctgcggt aatcagtcaa gcgcgcccgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyleA.00184.a.A1.PS01437
| PURIFICATION DATe: | 3/27/2012 |
| CONCENTRATION: | 18.1mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQQGNTATL ILLRHGESDW NARNLFTGWV DVGLTDKGRA EAVRSGELLA EHNLLPDVLY TSLLRRAITT AHLALDTADW LWIPVRRSWR LNERHYGALQ GLDKAVTKAR YGEERFMAWR RSYDTPPPPI EKGSEFSQDA DPRYTDIGGG PLTECLADVV TRFLPYFTDV IVPDLRTGRT VLIVAHGNSL RALVKHLDEM SDDEVVGLNV PTGIPLRYDL DADLRPVVPG GTYLDPEAAA AVISQARP
Validated NT Sequence
ttattacggg cgcgcttgac tgattaccgc agcggccgcc tcggggtcga ggtaggtacc accgggtacc accggacgca gatcggcatc tagatcatag cgcagcggaa tgccagtcgg aacgttaagc ccgacgacct cgtcgtcgga catctcgtct aggtgtttga ccagcgcgcg cagcgagttg ccgtgcgcca caatgagcac cgttctgccg gtccgcagat cggggactat cacgtcggtg aaatacggca ggaagcgggt caccacatca gccaggcatt cggtcagcgg cccgccgcca atgtcggtgt agcgagggtc agcatcctgg ctgaactcgc tacccttctc gatcggtggt ggcggagtgt cgtagctgcg gcgccaagcc atgaatcgtt cttcgccgta gcgggccttg gtcacggcct tgtccagacc ctgaagcgcg ccgtagtgac gttcattgag ccgccagctg cgacggaccg gaatccatag ccagtcggcg gtgtctaacg ccagatgcgc agtggtgata gcgcggcgca acagcgacgt gtacaacacg tcgggcaaca gattgtgctc ggcaagcaac tcaccgctgc ggaccgcctc cgctcgaccc ttgtcggtta gcccgacgtc cacccaaccg gtgaacagat tccgggcatt ccagtcactc tcgccatgac ggagtagaat cagcgtagca gtgttaccct gttgcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQQGNTATL ILLRHGESDW NARNLFTGWV DVGLTDKGRA EAVRSGELLA EHNLLPDVLY TSLLRRAITT AHLALDTADW LWIPVRRSWR LNERHYGALQ GLDKAVTKAR YGEERFMAWR RSYDTPPPPI EKGSEFSQDA DPRYTDIGGG PLTECLADVV TRFLPYFTDV IVPDLRTGRT VLIVAHGNSL RALVKHLDEM SDDEVVGLNV PTGIPLRYDL DADLRPVVPG GTYLDPEAAA AVISQARP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAACAGGGT AACACTGCTA CGCTGATTCT ACTCCGTCAT GGCGAGAGTG ACTGGAATGC CCGGAATCTG TTCACCGGTT GGGTGGACGT CGGGCTAACC GACAAGGGTC GAGCGGAGGC GGTCCGCAGC GGTGAGTTGC TTGCCGAGCA CAATCTGTTG CCCGACGTGT TGTACACGTC GCTGTTGCGC CGCGCTATCA CCACTGCGCA TCTGGCGTTA GACACCGCCG ACTGGCTATG GATTCCGGTC CGTCGCAGCT GGCGGCTCAA TGAACGTCAC TACGGCGCGC TTCAGGGTCT GGACAAGGCC GTGACCAAGG CCCGCTACGG CGAAGAACGA TTCATGGCTT GGCGCCGCAG CTACGACACT CCGCCACCAC CGATCGAGAA GGGTAGCGAG TTCAGCCAGG ATGCTGACCC TCGCTACACC GACATTGGCG GCGGGCCGCT GACCGAATGC CTGGCTGATG TGGTGACCCG CTTCCTGCCG TATTTCACCG ACGTGATAGT CCCCGATCTG CGGACCGGCA GAACGGTGCT CATTGTGGCG CACGGCAACT CGCTGCGCGC GCTGGTCAAA CACCTAGACG AGATGTCCGA CGACGAGGTC GTCGGGCTTA ACGTTCCGAC TGGCATTCCG CTGCGCTATG ATCTAGATGC CGATCTGCGT CCGGTGGTAC CCGGTGGTAC CTACCTCGAC CCCGAGGCGG CCGCTGCGGT AATCAGTCAA GCGCGCCCGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA