MythA.00184.a

2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (A0QR00 homolog)

CENTER ID:	MythA.00184.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.00184.a.A1.GE28711	Full length( MythA.00184.a )		1	363

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1078020.3.peg.3027
RefSeq:	WP_003926560.1
UniProt:	G7CHI6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAAGVWRVI RMCRGGGDRP DVAPWAHVRR DHRTFTAAIA RSPQPSHTHR SDRTADMRPG PHEMISAMGN GATLILLRHG ESDWNAKNLF TGWVDVDLTD KGRAEAVRAG ELMAELDRLP DVVYTSLLRR AITTANLALD RADRHWIPVH RSWRLNERHY GALQGLNKAE TKEKYGEEQF MAWRRSYDTP PPPIERGSTY SQDGDPRYAD IDGGPLTECL ADVVARFVPY YEQTIVPDLR AGKTVLIAAH GNSLRALVKY LDGMSDEEVV GLNIPTGIPL RYDLDENLKP KVVGGQYLXX XXXXXXXXXX XXXXXXXXXX XXGPGLGEQR VNSGRISQLT GVTCPVSAAR SWDDVHPLRT IFA

NT Sequence

GTGGCCGCGG CGGGCGTGTG GCGGGTGATT CGAATGTGCC GTGGTGGTGG CGATCGGCCC GATGTCGCAC CGTGGGCGCA CGTTCGCCGT GACCATCGCA CGTTCACCGC AGCCATCGCA CGTTCACCGC AGCCATCGCA CACTCACCGC AGTGACCGCA CGGCGGACAT GCGACCCGGG CCACATGAGA TGATTTCGGC CATGGGAAAC GGTGCCACGC TGATTCTGCT CCGCCACGGT GAGAGTGACT GGAACGCCAA GAACCTGTTC ACCGGCTGGG TCGACGTCGA CCTCACCGAC AAGGGCCGCG CCGAAGCGGT GCGTGCCGGG GAGTTGATGG CCGAGCTCGA CCGGCTGCCC GACGTGGTCT ACACCTCGCT GCTGCGCCGC GCCATCACCA CCGCCAACCT GGCGCTGGAC CGGGCGGACC GGCACTGGAT CCCCGTCCAC CGCAGCTGGC GGCTCAACGA ACGCCACTAC GGCGCGCTGC AGGGGCTGAA CAAGGCCGAG ACCAAGGAGA AATACGGCGA AGAGCAGTTC ATGGCCTGGC GGCGCAGCTA CGACACCCCG CCCCCACCCA TCGAGCGGGG CAGCACGTAC AGCCAGGACG GCGACCCGCG CTACGCCGAC ATCGACGGCG GTCCGCTGAC CGAATGCCTG GCCGACGTGG TCGCCCGGTT CGTGCCCTAC TACGAGCAGA CCATCGTGCC GGATCTGCGG GCCGGCAAGA CGGTGCTGAT CGCCGCGCAC GGCAACTCGC TGCGGGCGCT GGTGAAGTAC CTCGACGGCA TGTCCGACGA GGAGGTCGTC GGCCTCAACA TCCCGACCGG GATCCCGCTT CGCTACGACC TGGACGAGAA CCTCAAACCG AAGGTCGTCG GCGGGCAGTA TCTGNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGGGC CCGGCCTGGG CGAACAGCGG GTGAACAGCG GCCGAATTTC TCAGCTCACG GGTGTGACGT GTCCCGTTTC GGCCGCGCGC AGCTGGGATG ACGTTCACCC GTTGCGTACG ATTTTCGCG

Details for MythA.00184.a.A1.GE28711

HARVESTED ON:	3/1/2010
SEQUENCED ON:	5/19/2010
EXPECTED MW:	42kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	94
PERCENT COVERAGE:	87

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVAAAGVWR VIRMCRGGGD RPDVAPWAHV RRDHRTFTAA IARSPQPSHT HRSDRTADMR PGPHEMISAM GNGATLILLR HGESDWNAKN LFTGWVDVDL TDKGRAEAVR AGELMAELDR LPDVVYTSLL RRAITTANLA LDRADRHWIP VHRSWRLNER HYGALQGLNK AETKEKYGEE QFMAWRRSYD TPPPPIERGS TYSQDGDPRY ADIDGGPLTE CLADVVARFV PYYEQTIVPD LRAGKTVLIA AHGNSLRALV KYLDGMSDEE VVGLNIPTGI PLRYDLDENL KPKVVGGQYL DPEAAAAGAA AVAAQGAK

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg ccgcggcggg cgtgtggcgg gtgattcgaa tgtgccgtgg tggtggcgat cggcccgatg tcgcaccgtg ggcgcacgtt cgccgtgacc atcgcacgtt caccgcagcc atcgcacgtt caccgcagcc atcgcacact caccgcagtg accgcacggc ggacatgcga cccgggccac atgagatgat ttcggccatg ggaaacggtg ccacgctgat tctgctccgc cacggtgaga gtgactggaa cgccaagaac ctgttcaccg gctgggtcga cgtcgacctc accgacaagg gccgcgccga agcggtgcgt gccggggagt tgatggccga gctcgaccgg ctgcccgacg tggtctacac ctcgctgctg cgccgcgcca tcaccaccgc caacctggcg ctggaccggg cggaccggca ctggatcccc gtccaccgca gctggcggct caacgaacgc cactacggcg cgctgcaggg gctgaacaag gccgagacca aggagaaata cggcgaagag cagttcatgg cctggcggcg cagctacgac accccgcccc cacccatcga gcggggcagc acgtacagcc aggacggcga cccgcgctac gccgacatcg acggcggtcc gctgaccgaa tgcctggccg acgtggtcgc ccggttcgtg ccctactacg agcagaccat cgtgccggat ctgcgggccg gcaagacggt gctgatcgcc gcgcacggca actcgctgcg ggcgctggtg aagtacctcg acggcatgtc cgacgaggag gtcgtcggcc tcaacatccc gaccgggatc ccgcttcgct acgacctgga cgagaacctc aaaccgaagg tcgtcggcgg gcagtatctg gacccggagg ccgccgcggc cggagccgcc gccgtcgcgg cccagggcgc gaagtaacac cggcgggccc ggcctgggcg aacagcgggt gaacagcggc cgaatttctc agctcacggg tgtgacgtgt cccgtttcgg ccgcgcgcag ctgggatgac gttcacccgt tgcgtacgat tttcgcgt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccgcg gcgggcgtgt ggcgggtgat tcgaatgtgc cgtggtggtg gcgatcggcc cgatgtcgca ccgtgggcgc acgttcgccg tgaccatcgc acgttcaccg cagccatcgc acgttcaccg cagccatcgc acactcaccg cagtgaccgc acggcggaca tgcgacccgg gccacatgag atgatttcgg ccatgggaaa cggtgccacg ctgattctgc tccgccacgg tgagagtgac tggaacgcca agaacctgtt caccggctgg gtcgacgtcg acctcaccga caagggccgc gccgaagcgg tgcgtgccgg ggagttgatg gccgagctcg accggctgcc cgacgtggtc tacacctcgc tgctgcgccg cgccatcacc accgccaacc tggcgctgga ccgggcggac cggcactgga tccccgtcca ccgcagctgg cggctcaacg aacgccacta cggcgcgctg caggggctga acaaggccga gaccaaggag aaatacggcg aagagcagtt catggcctgg cggcgcagct acgacacccc gcccccaccc atcgagcggg gcagcacgta cagccaggac ggcgacccgc gctacgccga catcgacggc ggtccgctga ccgaatgcct ggccgacgtg gtcgcccggt tcgtgcccta ctacgagcag accatcgtgc cggatctgcg ggccggcaag acggtgctga tcgccgcgca cggcaactcg ctgcgggcgc tggtgaagta cctcgacggc atgtccgacg aggaggtcgt cggcctcaac atcccgaccg ggatcccgct tcgctacgac ctggacgaga acctcaaacc gaaggtcgtc ggcgggcagt atctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggg cccggcctgg gcgaacagcg ggtgaacagc ggccgaattt ctcagctcac gggtgtgacg tgtcccgttt cggccgcgcg cagctgggat gacgttcacc cgttgcgtac gattttcgcg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa