MytuD.18523.a

Protein tyrosine kinase (PtkA)

CENTER ID: MytuD.18523.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18523.a.B1.GE36549 full length 1 291
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2490
RefSeq: NP_216748.2
RefSeq: NP_336762.1
TubercuList: Rv2232
MTB Network Portal: Rv2232
UniProt: P9WPI9

Orthologues for Rv2232

Species % Identity % Coverage Clones Proteins Structures
smegmatis 71 73 1 2 0
thermoresistibile 71 73 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSPRERRPA SQAPRLSRRP PAHQTSRSSP DTTAPTGSGL SNRFVNDNGI VTDTTASGTN CPPPPRAAAR RASSPGESPQ LVIFDLDGTL TDSARGIVSS FRHALNHIGA PVPEGDLATH IVGPPMHETL RAMGLGESAE EAIVAYRADY SARGWAMNSL FDGIGPLLAD LRTAGVRLAV ATSKAEPTAR RILRHFGIEQ HFEVIAGAST DGSRGSKVDV LAHALAQLRP LPERLVMVGD RSHDVDGAAA HGIDTVVVGW GYGRADFIDK TSTTVVTHAA TIDELREALG V
NT Sequence
gtgtcttcgc ctcgtgaacg ccgacccgct tcgcaggcgc ccagactttc gcgtcgacca cctgctcacc aaacttcgcg atcatcgcct gataccacag cgccaacggg tagcggtttg tccaaccgct tcgtcaacga caatgggatc gtgaccgaca cgaccgcgag cgggaccaat tgcccgcctc ctccacgcgc cgccgcacgg cgcgcatcgt cgccgggtga atcgccgcag ctggtgatct tcgatctgga cggcacgctg accgactcgg cgcgcggaat cgtatccagc ttccgacacg cgctcaacca catcggtgcc ccagtacccg aaggcgacct ggccactcac atcgtcggcc cgcccatgca tgagacgctg cgcgccatgg ggctcggcga atccgccgag gaggcgatcg tagcctaccg ggccgactac agcgcccgcg gttgggcgat gaacagcttg ttcgacggga tcgggccgct gctggccgac ctgcgcaccg ccggtgtccg gctggccgtc gccacctcca aggcagagcc gaccgcacgg cgaatcctgc gccacttcgg aattgagcag cacttcgagg tcatcgcggg cgcgagcacc gatggctcgc gaggcagcaa ggtcgacgtg ctggcccacg cgctcgcgca gctgcggccg ctacccgagc ggttggtgat ggtcggcgac cgcagccacg acgtcgacgg ggcggccgcg cacggcatcg acacggtggt ggtcggctgg ggctacgggc gcgccgactt tatcgacaag acctccacca ccgtcgtgac gcatgccgcc acgattgacg agctgaggga ggcgctaggt gtctga
Details for MytuD.18523.a.B1.GE36549
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 32kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 91
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMV SSPRERRPAS QAPRLSRRPP AHQTSRSSPD TTAPTGSGLS NRFVNDNGIV TDTTASGTNC PPPPRAAARR ASSPGESPQL VIFDLDGTLT DSARGIVSSF RHALNHIGAP VPEGDLATHI VGPPMHETLR AMGLGESAEE AIVAYRADYS ARGWAMNSLF DGIGPLLADL RTAGVRLAVA TSKAEPTARR ILRHFGIEQH FEVIAGASTD GSRGSKVDVL AHALAQLRPL PERLVMVGDR SHDVDXXPRT ASTRWWSAGA TGAPTLSTRP PPPS
Validated NT Sequence
tttgtnnnnn ttaagaagaa ganataccat ggctcaccac caccaccacc atatggtgtc ttcgcctcgt gaacgccgac ccgcttcgca ggcgcccaga ctttcgcgtc gaccacctgc tcaccaaact tcgcgatcat cgcctgatac cacagcgcca acgggtagcg gtttgtccaa ccgcttcgtc aacgacaatg ggatcgtgac cgacacgacc gcgagcggga ccaattgccc gcctcctcca cgcgccgccg cacggcgcgc atcgtcgccg ggtgaatcgc cgcagctggt gatcttcgat ctggacggca cgctgaccga ctcggcgcgc ggaatcgtat ccagcttccg acacgcgctc aaccacatcg gtgccccagt acccgaaggc gacctggcca ctcacatcgt cggcccgccc atgcatgaga cgctgcgcgc catggggctc ggcgaatccg ccgaggaggc gatcgtagcc taccgggccg actacagcgc ccgcggttgg gcgatgaaca gcttgttcga cgggatcggg ccgctgctgg ccgacctgcg caccgccggt gtccggctgg ccgtcgccac ctccaaggca gagccgaccg cacggcgaat cctgcgccac ttcggaattg agcagcactt cgaggtcatc gcgggcgcga gcaccgatgg ctcgcgaggc agcaaggtcg acgtgctggc ccacgcgctc gcgcagctgc ggccgctacc cgagcggttg gtgatggtcg gcgaccgcag ccacgacgtc gacnnnnggc cgcgcacggc atcgacacgg tggtggtcgg ctggggctac nggcgcgccg actttatcga caagacctcc accaccgtcg tgacgcatgc cgccacgatt gacnagctga gggaggcnct agnngtctaa gtgagtaana nagganccnn nnnnnnnnnn cnnnnnnnan nnnnnnnnnn nnnnnnntnt nnnntnnnnn nnnnnnnccc ncnnacnncn nnnnnnnc
Expected Protein Sequence
MAHHHHHHMV SSPRERRPAS QAPRLSRRPP AHQTSRSSPD TTAPTGSGLS NRFVNDNGIV TDTTASGTNC PPPPRAAARR ASSPGESPQL VIFDLDGTLT DSARGIVSSF RHALNHIGAP VPEGDLATHI VGPPMHETLR AMGLGESAEE AIVAYRADYS ARGWAMNSLF DGIGPLLADL RTAGVRLAVA TSKAEPTARR ILRHFGIEQH FEVIAGASTD GSRGSKVDVL AHALAQLRPL PERLVMVGDR SHDVDGAAAH GIDTVVVGWG YGRADFIDKT STTVVTHAAT IDELREALGV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgt cttcgcctcg tgaacgccga cccgcttcgc aggcgcccag actttcgcgt cgaccacctg ctcaccaaac ttcgcgatca tcgcctgata ccacagcgcc aacgggtagc ggtttgtcca accgcttcgt caacgacaat gggatcgtga ccgacacgac cgcgagcggg accaattgcc cgcctcctcc acgcgccgcc gcacggcgcg catcgtcgcc gggtgaatcg ccgcagctgg tgatcttcga tctggacggc acgctgaccg actcggcgcg cggaatcgta tccagcttcc gacacgcgct caaccacatc ggtgccccag tacccgaagg cgacctggcc actcacatcg tcggcccgcc catgcatgag acgctgcgcg ccatggggct cggcgaatcc gccgaggagg cgatcgtagc ctaccgggcc gactacagcg cccgcggttg ggcgatgaac agcttgttcg acgggatcgg gccgctgctg gccgacctgc gcaccgccgg tgtccggctg gccgtcgcca cctccaaggc agagccgacc gcacggcgaa tcctgcgcca cttcggaatt gagcagcact tcgaggtcat cgcgggcgcg agcaccgatg gctcgcgagg cagcaaggtc gacgtgctgg cccacgcgct cgcgcagctg cggccgctac ccgagcggtt ggtgatggtc ggcgaccgca gccacgacgt cgacggggcg gccgcgcacg gcatcgacac ggtggtggtc ggctggggct acgggcgcgc cgactttatc gacaagacct ccaccaccgt cgtgacgcat gccgccacga ttgacgagct gagggaggcg ctaggtgtct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat