SSGCID: Target Page

MythA.18523.a

HAD family hydrolase

CENTER ID:	MythA.18523.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.18523.a.B1.GE36449	full length		1	241

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.1177
UniProt:	G7CDX7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTETRDDTAA VPAGAVDAAR RPVDPALRPE LVIFDLDGTL TDSAEGIVSS FRHALASVGA AVPDGDLAAR IIGPPMHHTL RSLGLGDQTD AAIDAYRQDY RARGWAMNRV YDGIPELLAD LRAAEVRLAV ATSKAEPTAQ RILAHFGLAD HFEFIAGASV DGSRAAKADV LARALQALQP LPERVLMVGD RCHDVEGAAA HGIDTVVVGW GYGRDDFAHE DAIPAYAHVA TVAELREVLS V

NT Sequence

gtgactgaga cgcgcgacga cacggccgcc gtgccggcgg gtgccgtgga cgcggcccgc cggcccgtgg atccggcgct gcggccggag ttggtgatct tcgatctgga cggcacgctg accgactcgg cggagggcat cgtgtccagc tttcgccatg ccctggcctc ggtgggcgcg gcggtgcccg acggcgacct ggccgcccgg atcatcggcc cgccgatgca ccacacgctg cgctcgctgg ggctgggcga tcagaccgac gccgcgatcg acgcgtaccg gcaggattac cgcgcccgcg gatgggccat gaaccgggtc tacgacggca ttccggagct gttggccgac ctgcgcgccg ccgaggtgcg gctcgcggtg gccacctcga aggccgaacc gaccgcgcag cgcatcctgg cccatttcgg gttggccgat cacttcgagt tcatcgcggg ggccagtgtg gacggttccc gcgccgccaa ggccgatgtg ctggcccgtg cgctgcaggc gctgcagccg ctgcccgagc gggtgctgat ggtgggtgac cgctgccacg acgtggaggg cgccgcggcg cacggcatcg acaccgtcgt cgtcggatgg ggctacggcc gggacgattt cgcccatgag gacgccatcc cggcctatgc gcacgtggcc accgtcgcgg agcttcgtga ggtgttgagt gtctga

Details for MythA.18523.a.B1.GE36449

HARVESTED ON:	11/21/2012
SEQUENCED ON:	11/27/2012
EXPECTED MW:	26kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV TETRDDTAAV PAGAVDAARR PVDPALRPEL VIFDLDGTLT DSAEGIVSSF RHALASVGAA VPDGDLAARI IGPPMHHTLR SLGLGDQTDA AIDAYRQDYR ARGWAMNRVY DGIPELLADL RAAEVRLAVA TSKAEPTAQR ILAHFGLADH FEFIAGASVD GSRAAKADVL ARALQALQPL PERVLMVGDR CHDVEGAAAH GIDTVVVGWG YGRDDFAHED AIPAYAHVAT VAELREVLSV

Validated NT Sequence

atcttactca cttagacact caacacctca cgaagctccg cgacggtggc cacgtgcgca taggccggga tggcgtcctc atgggcgaaa tcgtcccggc cgtagcccca tccgacgacg acggtgtcga tgccgtgcgc cgcggcgccc tccacgtcgt ggcagcggtc acccaccatc agcacccgct cgggcagcgg ctgcagcgcc tgcagcgcac gggccagcac atcggccttg gcggcgcggg aaccgtccac actggccccc gcgatgaact cgaagtgatc ggccaacccg aaatgggcca ggatgcgctg cgcggtcggt tcggccttcg aggtggccac cgcgagccgc acctcggcgg cgcgcaggtc ggccaacagc tccggaatgc cgtcgtagac ccggttcatg gcccatccgc gggcgcggta atcctgccgg tacgcgtcga tcgcggcgtc ggtctgatcg cccagcccca gcgagcgcag cgtgtggtgc atcggcgggc cgatgatccg ggcggccagg tcgccgtcgg gcaccgccgc gcccaccgag gccagggcat ggcgaaagct ggacacgatg ccctccgccg agtcggtcag cgtgccgtcc agatcgaaga tcaccaactc cggccgcagc gccggatcca cgggccggcg ggccgcgtcc acggcacccg ccggcacggc ggccgtgtcg tcgcgcgtct cagtcaccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga ctgagacgcg cgacgacacg gccgccgtgc cggcgggtgc cgtggacgcg gcccgccggc ccgtggatcc ggcgctgcgg ccggagttgg tgatcttcga tctggacggc acgctgaccg actcggcgga gggcatcgtg tccagctttc gccatgccct ggcctcggtg ggcgcggcgg tgcccgacgg cgacctggcc gcccggatca tcggcccgcc gatgcaccac acgctgcgct cgctggggct gggcgatcag accgacgccg cgatcgacgc gtaccggcag gattaccgcg cccgcggatg ggccatgaac cgggtctacg acggcattcc ggagctgttg gccgacctgc gcgccgccga ggtgcggctc gcggtggcca cctcgaaggc cgaaccgacc gcgcagcgca tcctggccca tttcgggttg gccgatcact tcgagttcat cgcgggggcc agtgtggacg gttcccgcgc cgccaaggcc gatgtgctgg cccgtgcgct gcaggcgctg cagccgctgc ccgagcgggt gctgatggtg ggtgaccgct gccacgacgt ggagggcgcc gcggcgcacg gcatcgacac cgtcgtcgtc ggatggggct acggccggga cgatttcgcc catgaggacg ccatcccggc ctatgcgcac gtggccaccg tcgcggagct tcgtgaggtg ttgagtgtct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat